scholarly journals Oxidative stress parameters and inflammatory and immune mediators as markers of the severity of sepsis

2016 ◽  
Vol 10 (10) ◽  
pp. 1045-1052 ◽  
Author(s):  
Isil Bavunoglu ◽  
Habibe Genc ◽  
Dildar Konukoglu ◽  
Hayriye Cicekci ◽  
Volkan Sozer ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Severe Sepsis ◽  
Adhesion Molecule ◽  
Inflammatory Mediators ◽  
Paraoxonase 1 ◽  
Pon1 Activity ◽  
Enzyme Linked Immunosorbent Assay ◽  
Oxidative Stress Parameters ◽  
Tnf Α ◽  
Stress Parameters

Introduction: Sepsis is a complex inflammatory syndrome with diverse etiology and wide spectrum of severity. The aim of this study was to investigate whether inflammatory mediators, in comparison with oxidative parameters, are associated with severity of sepsis. Methodology: Plasma neopterin, adenosine deaminase (ADA), vascular cell adhesion molecule (VCAM), intracellular adhesion molecule (ICAM), interleukin (IL)-1, IL-6, and tumor necrosis factor alpha (TNF-α), as inflammatory mediators, and serum nitric oxide (NOx), nitrotyrosine (NT), oxidized LDL (oxLDL) levels, serum paraoxonase 1 (PON1) activity, and erythrocyte glutathione (GSH) levels as oxidative stress parameters of 12 patients with mild sepsis, 25 patients with severe sepsis, and 20 healthy control subjects were evaluated. NOx, GSH levels and PON1 activity were determined by colorimetric methods, whereas neopterin, VCAM, ICAM, IL-1, IL-6, TNF-α, NT, and oxLDL levels were measured by enzyme-linked immunosorbent assay (ELISA). Results: All parameters in mild and severe sepsis were significantly different from those of healthy subjects, except ADA activities. Patients with severe sepsis exhibited higher IL-6, TNF-α, NT, and oxLDL levels than patients with mild sepsis. GSH (98%, 98%), oxLDL (98%, 98%), VCAM-1 (99%, 99%), and ICAM-1 (99%, 99%) have much more sensitivitiy and specificity in sepsis. Conclusions: Our results suggest that the oxidative stress and inflammatory response in patients with sepsis were increased and that serum IL-6, TNF-α, NT, and oxLDL levels were correlated with the severity of sepsis. Therefore, increases in these parameters may contribute to the dysfunction or failure of one or more organs, or even death, in sepsis.

Decreased paraoxonase 1 activity and increased oxidative stress in low lead−exposed workers

2011 ◽  
Vol 30 (9) ◽  
pp. 1196-1203 ◽  
Author(s):  
Thinnakorn Permpongpaiboon ◽  
Amar Nagila ◽  
Phannee Pidetcha ◽  
Kulwadee Tuangmungsakulchai ◽  
Soontharee Tantrarongroj ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Lead Exposure ◽  
Linear Regression Analysis ◽  
Exposed Group ◽  
Paraoxonase 1 ◽  
Pon1 Activity ◽  
Control Group ◽  
Oxidative Stress Parameters ◽  
Exposed Workers ◽  
Stress Parameters

Paraoxonase 1 (PON1) has been proposed as an antioxidant enzyme. Although lead-inhibited PON1 activity has been demonstrated mostly based on in vitro experiments, it is uncertain whether this phenomenon is relevant in pathogenesis of lead-induced oxidative stress in the lead exposure. We examined associations of blood lead levels (BLL) and PON1 activity along with oxidative stress parameters in lead exposure workers. We determined malondialdehyde (MDA), conjugated diene (CD), total peroxides (TP), total antioxidant status (TAS), the oxidative stress index (OSI), and PON1 activity in earthenware factory workers ( n = 60) and control subjects ( n = 65). The lead-exposed group significantly increased lipid peroxidation parameters and OSI compared to the control group ( p < 0.001). The lead-exposed group had significantly decreased PON1 activity and TAS levels compared to the control group ( p < 0.001). Multiple linear regression analysis revealed that BLL were significantly correlated with decreased TAS ( r = −0.496) and PON1 activity ( r = −0.434), but with increased CD ( r = 0.694), TP ( r = 0.614), MDA ( r = 0.788), and OSI ( r = 0.722). Interestingly, BLL at 10 µg/dL significantly decreased PON1 activity and increased oxidative stress parameters with insignificant changes in other biochemical and hematological parameters. Altogether, the reduction of PON1 activity may associate in an imbalance in pro-oxidants and antioxidants, leading to oxidative damage in lead-exposed workers even at low BLL.

Oxydative stress markers and cytokine levels in rosuvastatin-medicated hypercholesterolemia patients

2018 ◽  
Vol 44 (4) ◽  
pp. 530-538
Author(s):  
Aysun Çetin ◽  
İhsan Çetin ◽  
Semih Yılmaz ◽  
Ahmet Şen ◽  
Göktuğ Savaş ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Interleukin 1 ◽  
Paraoxonase 1 ◽  
Enzyme Linked Immunosorbent Assay ◽  
Control Group ◽  
Phenotypic Effect ◽  
Necrosis Factor Alpha ◽  
Stress Markers ◽  
Tnf Α ◽  
Before And After

Abstract Background Limited research is available concerning the relationship between oxidative stress and inflammation parameters, and simultaneously the effects of rosuvastatin on these markers in patients with hypercholesterolemia. We aimed to investigate the connection between cytokines and oxidative stress markers in patients with hypercholesterolemia before and after rosuvastatin treatment. Methods The study consisted of 30 hypercholesterolemic patients diagnosed with routine laboratory tests and 30 healthy participants. The lipid parameters, interleukin-1 beta (IL-1β), interleukin-6 (IL-6), tumor necrosis factor-alpha (TNF-α), paraoxonase-1 (PON1) and malondialdehyde (MDA) levels in controls and patients with hypercholesterolemia before and after 12-week treatment with rosuvastatin (10 mg/kg/day), were analyzed by means of enzyme-linked immunosorbent assay. Results It was found that a 12-week cure with rosuvastatin resulted in substantial reductions in IL-1β, IL-6 and TNF-α and MDA levels as in rising activities of PON1 in patients with hypercholesterolemia. Before treatment, the PON1 levels were significantly negatively correlated with TNF-α and IL-6 in control group, while it was positively correlated with TNF-α in patients. Conclusion Our outcomes provide evidence of protected effect of rosuvastatin for inflammation and oxidative damage. It will be of great interest to determine whether the correlation between PON1 and cytokines has any phenotypic effect on PON1.

scholarly journals Oxidative Stress and Neuronal Injury After Cannabis and Ketamine Administration

2021 ◽  
Vol 18 ◽  
pp. 126-135
Author(s):  
Omar M. E. Abdel-Salam ◽  
Eman R. Youness ◽  
Amany Ameen Sleem ◽  
Enayat A. Omara
Keyword(s):  
Oxidative Stress ◽  
Inflammatory Mediators ◽  
Cannabis Sativa ◽  
Paraoxonase 1 ◽  
Saline Control ◽  
Control Group ◽  
Neuronal Necrosis ◽  
Tnf Α ◽  
Markers Of Oxidative Stress ◽  
Factor Α

Cannabis sativa and ketamine are common substances of abuse causing psychotic events and neurodegeneration. In this study, the effect of pretreatment with Cannabis sativa extract on oxidative stress, inflammatory mediators and brain damage induced by ketamine was investigated. Rats were treated with subcutaneous injections of cannabis extract (10, 20, 30 or 40 mg/kg; expressed as Δ9-THC content) daily for three weeks and then in combination with ketamine (15 mg/kg, intraperitoneally) for another 5 days. Rats were tested for biochemical markers of oxidative stress including malondialdehyde (MDA) reduced glutathione (GSH), and nitric oxide (NO) concentrations in brain. Paraoxonase-1 (PON-1) activity, and levels of the proinflammatory cytokines, interleukin-1β (IL-1β), and tumour necrosis factor-α (TNF-α) in brain were also determined at the end of treatment period. Results indicated that compared with the saline control group, ketamine induced significant elevation in brain MDA and NO, which was accompanied by depletion of GSH and inhibition of PON-1 activity. Ketamine also significantly increased brain IL-1β and TNF-α and induced neuronal necrosis, apoptosis and vacuolation. Cannabis sativa (20-40 mg/kg) pretreated rats showed lower levels of oxidative stress and inflammation and doses of 30 or 40 mg/kg slightly reduced neuronal apoptosis and necrosis. These findings suggest that cannabis constituents do not enhance the neurotoxic effects of ketamine and might partly counteract the effects of ketamine-induced NMDA antagonism by reducing the release of free radicals and inflammatory mediators in brain

Alterations in Inflammatory Mediators, Oxidative Stress Parameters and Energetic Metabolism in the Brain of Sepsis Survivor Rats

2010 ◽  
Vol 36 (2) ◽  
pp. 304-311 ◽  
Author(s):  
Clarissa M. Comim ◽  
Omar J. Cassol-Jr ◽  
Larissa S. Constantino ◽  
Francine Felisberto ◽  
Fabricia Petronilho ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Inflammatory Mediators ◽  
Energetic Metabolism ◽  
Oxidative Stress Parameters ◽  
Sepsis Survivor ◽  
The Brain ◽  
Stress Parameters

Serum oxidative stress parameters and paraoxonase-1 in children and adolescents exposed to passive smoking

2016 ◽  
Vol 59 (1) ◽  
pp. 68-73 ◽  
Author(s):  
Feyza Ustabaş Kahraman ◽  
Emel Torun ◽  
Nurcan Keskin Osmanoğlu ◽  
Suera Oruçlu ◽  
Ömer Faruk Özer
Keyword(s):  
Oxidative Stress ◽  
Children And Adolescents ◽  
Passive Smoking ◽  
Paraoxonase 1 ◽  
Oxidative Stress Parameters ◽  
Stress Parameters

The effect of tobacco smoke on oxytocin concentrations and selected oxidative stress parameters in plasma during pregnancy and post-partum – an experimental model

2016 ◽  
Vol 36 (2) ◽  
pp. 135-145 ◽  
Author(s):  
M Napierala ◽  
TA Merritt ◽  
J Mazela ◽  
K Jablecka ◽  
I Miechowicz ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Experimental Model ◽  
Tobacco Smoke ◽  
Smoke Exposure ◽  
Tobacco Smoke Exposure ◽  
Trolox Equivalent Antioxidant Capacity ◽  
Enzyme Linked Immunosorbent Assay ◽  
Lactation Period ◽  
Oxidative Stress Parameters ◽  
Stress Parameters

Background: Tobacco smoking is a serious threat to life and health of society. Among the most vulnerable to the toxic effects of tobacco smoke are foetuses and newborns. The objective of the research was to assess the impact of tobacco smoke exposure on oxytocin levels and biochemical oxidative stress parameters during pregnancy and after birth in an experimental model. Methods: In the experiment, exposure to tobacco smoke of gravid and non-gravid rats was monitored. A reliable biomarker of exposure – cotinine – was used in the process and it was determined by means of high-performance liquid chromatography with diode array detection, which ensured high analytical accuracy and precision. Determination of oxytocin was performed by means of enzyme-linked immunosorbent assay. The levels of selected oxidative stress parameters: total protein concentration, uric acid, trolox equivalent antioxidant capacity, protein S-nitrosylation and lipid peroxidation (thiobarbituric acid reactive substances) were measured by spectrophotometric methods. Results and conclusions: The effect of prenatal and postnatal exposure to tobacco smoke was a lower medium body mass of rat foetuses and pups. Oxidative stress during pregnancy, additionally intensified by tobacco smoke exposure, led to adaptive changes in properties of plasmatic antioxidant barriers. Moreover, the disturbance of oxidoreductive balance by tobacco smoke affects oxytocin fluctuations, what was observed in this study during lactation period. Therefore, women who smoke may breastfeed their children less frequently and for a shorter period.

scholarly journals The efficacy of alpha-lipoic acid and/or gabapentin on the oxidant-antioxidant system in patients with diabetic polyneuropathy

2020 ◽  
Vol 8 (8) ◽  
pp. 2777
Author(s):  
Hatice Ferhan Komurcu ◽  
Pervin Baran ◽  
Ozcan Erel ◽  
Omer Anlar
Keyword(s):  
Oxidative Stress ◽  
Lipoic Acid ◽  
Diabetic Polyneuropathy ◽  
Serum Levels ◽  
Paraoxonase 1 ◽  
Stress Index ◽  
Alpha Lipoic Acid ◽  
Oxidative Stress Parameters ◽  
Significant Difference ◽  
Stress Parameters

Background: Diabetic peripheral sensorimotor polyneuropathy is the most common complication seen in patients with diabetes mellitus (DM). Oxidant system plays a crucial role in its physiopathology. We investigated the changes in the serum levels of total antioxidant status (TAS), total oxidant status (TOS), paraoxonase-1 (PON1) and oxidative stress index (OSI) to evaluate the antioxidant efficacy of alpha lipoic acid (ALA) and/or gabapentin in patients with diabetic polyneuropathy (DPN).Methods: Sixty-three type 2 DM patients with diabetic polyneuropathy (DPN) were enrolled in the study. Patients with DPN were divided into four groups in terms of their treatment: Group 1 consisted of treatment-naive patients; patients treated with ALA, gabapentin or combination of ALA and gabapentin comprised groups 2, 3, and 4, respectively. The patients received the medications for at least six weeks.  Serum levels of TAS, TOS, PON1 and OSI were analyzed.Results: No significant difference was observed between the groups according to the oxidative stress parameters studied.Conclusions: The use of ALA and/or gabapentin in patients with DPN did not significantly affect the oxidative stress parameters, including TAS, TOS, PON1, and OSI.

scholarly journals Presentation of cytokine profile in relation to oxidative stress parameters in patients with severe COVID-19: an observational pilot study

F1000Research ◽  
2021 ◽  
Vol 10 ◽  
pp. 719
Author(s):  
Marija Petrushevska ◽  
Dragica Zendelovska ◽  
Emilija Atanasovska ◽  
Aleksandar Eftimov ◽  
Katerina Spasovska
Keyword(s):  
Oxidative Stress ◽  
Care Setting ◽  
High Sensitivity ◽  
Photometric System ◽  
Oxidative Stress Parameters ◽  
Stress Markers ◽  
Tnf Α ◽  
Array Technology ◽  
Ifn Γ ◽  
Stress Parameters

Introduction: COVID-19 can be worsened by hyper-production of cytokines accompanied by increased level of oxidative stress. The aim of this study was to investigate the correlation between a set of cytokines and the markers of the oxidative stress. Methods: The levels of cytokines IL-2, IL-4, IL-6, IL8, IL-10, VEGF, IFN-γ, TNF-α, IL-1α, MCP-1 and EGF were determined by using High Sensitivity Evidence Investigator™ Biochip Array technology. The oxidative stress parameters (d-ROM, PAT, OS index) were measured in serum on FRAS5 analytical photometric system. Results: IL-6, IL-8, IL-10, VEGF, MCP-1 and EGF were significantly higher (p<0.05) in the patients with severe COVID-19 with increased levels of IL-2, IFN-g, TNF-a and IL-1α. The d-ROM, OS index, and PAT were significantly higher (p<0.05) in severe COVID-19 patients. IL-6 demonstrated the strongest correlation with all of the markers of the oxidative stress, d-ROM (r=0.9725, p=0.0001), PAT (r=0.5000, p=0.0001) and OS index (r=0.9593, p=0.012). Similar behavior was evidenced between IFN-g and d-ROM (r=0.4006, p=0.0001), PAT (r=0.6030, p=0.0001) and OS index (r=0.4298, p=0.012). Conclusion: The oxidative stress markers show good correlation with the tested cytokines which can be measured at the beginning of the disease in a primary care setting to predict the course of COVID-19.

Association of Dyslipidemia, Oxidative Stress, and Inflammation With Redox Status in VLDL, LDL, and HDL Lipoproteins in Patients With Renal Disease

Angiology ◽  
2018 ◽  
Vol 69 (10) ◽  
pp. 861-870 ◽  
Author(s):  
Milica Miljkovic ◽  
Aleksandra Stefanovic ◽  
Sanja Simic-Ogrizovic ◽  
Jelena Vekic ◽  
Natasa Bogavac-Stanojevic ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Renal Disease ◽  
Low Density Lipoprotein ◽  
Density Lipoprotein ◽  
Redox Status ◽  
Cardiovascular Complications ◽  
Paraoxonase 1 ◽  
Pon1 Activity ◽  
Enzyme Linked Immunosorbent Assay ◽  
Low Density

Some cardiovascular complications in patients with chronic kidney disease and end-stage renal disease may be caused by structurally and functionally modified lipoproteins. Redox status (advanced oxidation protein products [AOPPs]), prooxidant–antioxidant balance, total protein sulfhydryl (SH-groups), and paraoxonase 1 (PON1) activity were assessed in 77 renal patients and 20 controls. Lipoproteins were isolated using ultracentrifugation. PON1, PON3, and pentraxin-3 concentration were determined by enzyme-linked immunosorbent assay (ELISA). Dyslipidemia-Oxy-Inflammation (DOI) score was calculated as a sum of dyslipidemia, oxidative stress, and inflammation scores. The dyslipidemia score ( P < .001), oxy score ( P < .01), inflammation score (P < .001), and the DOI score ( P < .001) were higher in patient groups compared with controls. The very-low-density lipoprotein (VLDL) fraction contained the highest amount of AOPP ( P < .001) compared with other lipoprotein fractions in all groups. The low-density lipoprotein (LDL) fraction contained elevated AOPP in all groups compared with the high-density lipoprotein (HDL) fraction ( P < .001). Significant positive correlation was observed between AOPP in LDL fraction and DOI score (ρ = 0.510, P < .01). Dyslipidemia, oxidative stress, and inflammation play an interactive role in renal disease and are mutually associated with redox status in VLDL, LDL, and HDL lipoproteins in plasma of renal patients.

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