Apoptotic with Double-Staining Test, P53, and Cyclooxygenase-2 to Proliferation Colon Cancer Cell (WiDr) of Dolichol in Three Mangrove Leaves

BACKGROUND: Mangroves secondary metabolites are mostly consisting of sterols, ubiquinones, isoprenoids, and polyisoprenoids. Polyisoprenoid is divided into two types, namely, polyprenol and dolichol, which has been reported to have biological and pharmacological activities. AIM: This research was aimed to analyze apoptosis 48 h with double staining and immunocytochemistry (ICC) 48 h of P53 and cyclooxygenase-2 (COX-2) gene expression from chemical constituents of dolichol in three mangrove leaves of Ceriops tagal, Nypa fruticans, and Rhizophora mucronata. METHODS: Apoptosis with the double-staining method was employed to analyze the genes expression in growth and development of cancer cells, P53, and COX-2 with ICC and flow cytometry method. The data were statistically analyzed using one-way ANOVA parametric statistical analysis followed by Duncan’s test. RESULTS: The result revealed that the increased apoptosis of samples C. tagal was 70% fluorescence orange, while N. fruticans and R. mucronata were 35% and 30% fluorescence orange, respectively. However, it was compared with the positive control; it produced orange fluorescent as much as 75%, suggesting that C. tagal have a position similar to 5-FU. Predominance dolichol in N. fruticans and C. tagal leaves led the expression gene of p53 to have 1.57% M1 phase, indicating the domination in G0-G1 phase (70–80%). Inhibit the expression for 48 h in p53 and COX-2 showing that n-hexane extract of C. tagal had the most percentage (80.733 ± 0.11%) to upregulate the p53 and less percentage (20.16 ± 1.19%) to downregulate the COX-2, indicating positive extract belong to N. fruticans and R. mucronata leaves. CONCLUSION: The present study confirmed the pharmacological properties of dolichol from three mangrove leaves as an anticancer of tumor suppressor genes and significantly proliferated of cancer cell growth from mangrove leaves.

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Toll-Like Receptor 4 (TLR4)/Cyclooxygenase-2 (COX-2) Regulates Prostate Cancer Cell Proliferation, Migration, and Invasion by NF-κB Activation

Medical Science Monitor ◽

10.12659/msm.906857 ◽

2018 ◽

Vol 24 ◽

pp. 5588-5597 ◽

Cited By ~ 7

Author(s):

Wei Wang ◽

Jiye Wang

Keyword(s):

Prostate Cancer ◽

Cell Proliferation ◽

Cancer Cell ◽

Prostate Cancer Cell ◽

Cyclooxygenase 2 ◽

Migration And Invasion ◽

Cancer Cell Proliferation ◽

Toll Like Receptor ◽

Toll Like Receptor 4 ◽

Cox 2

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Colon cancer cell growth inhibition by treatment with a selective cox-2 inhibitor

Gastroenterology ◽

10.1016/s0016-5085(98)82453-0 ◽

1998 ◽

Vol 114 ◽

pp. A601

Author(s):

AP Goldman ◽

CS Williams ◽

VP Williams ◽

RN DuBois

Keyword(s):

Colon Cancer ◽

Cell Growth ◽

Growth Inhibition ◽

Cancer Cell ◽

Colon Cancer Cell ◽

Cell Growth Inhibition ◽

Cancer Cell Growth ◽

Cox 2

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Somatostatin inhibits colon cancer cell growth through cyclooxygenase-2 downregulation

British Journal of Pharmacology ◽

10.1038/bjp.2008.268 ◽

2008 ◽

Vol 155 (2) ◽

pp. 198-209 ◽

Cited By ~ 17

Author(s):

R Colucci ◽

C Blandizzi ◽

N Ghisu ◽

T Florio ◽

M Del Tacca

Keyword(s):

Colon Cancer ◽

Cell Growth ◽

Cancer Cell ◽

Colon Cancer Cell ◽

Cyclooxygenase 2 ◽

Cancer Cell Growth

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THE INHIBITION OF POLYISOPRENOIDS FROM NYPA FRUTICANS LEAVES ON CYCLOOXYGENASE 2 EXPRESSION OF WIDR COLON CANCER CELLS

Asian Journal of Pharmaceutical and Clinical Research ◽

10.22159/ajpcr.2018.v11i8.26098 ◽

2018 ◽

Vol 11 (8) ◽

pp. 154 ◽

Cited By ~ 1

Author(s):

Dini Permata Sari ◽

Mohammad Basyuni ◽

Poppy Anjelisa Zaitun Hasibuan ◽

Ridha Wati

Keyword(s):

Colon Cancer ◽

Anticancer Activity ◽

Cancer Cells ◽

Colon Cancer Cell ◽

Cyclooxygenase 2 ◽

Colon Cancer Cells ◽

Chemopreventive Agents ◽

Nypa Fruticans ◽

Diphenyltetrazolium Bromide ◽

Cox 2

Objective: The objective of the study was to investigate the inhibitory activity of polyisoprenoids from Nypa fruticans leaves on the expression of cyclooxygenase 2 (COX-2) against colon cancer cells.Methods: Anticancer activity performed was tested by dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide method on colon cancer cell WiDr. The expression of COX-2 was observed by the immunocytochemistry method.Result: Polyisoprenoids from N. fruticans leaves exhibit anticancer activity on WiDr cells through inhibition of COX-2 expression with IC50 180.186±7.16 μg/ml.Conclusions: This study showed that polyisoprenoids from N. fruticans leaves promise chemopreventive agents for colon cancer through COX-2 inhibition.

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Gastroprotective activity of Loranthus acaciae flower extract in a rodent model of ethanol-induced ulcer

Applied Physiology Nutrition and Metabolism ◽

10.1139/apnm-2019-0166 ◽

2019 ◽

Vol 44 (12) ◽

pp. 1283-1288 ◽

Cited By ~ 2

Author(s):

Manal A. Abbas ◽

Yasser I. Kandil ◽

Ahmad M. Disi ◽

Sahar M. Jaffal

Keyword(s):

Chemical Constituents ◽

Cyclooxygenase 2 ◽

Surface Epithelium ◽

Control Group ◽

Spectrometric Analysis ◽

Glutathione Level ◽

Ulcer Index ◽

Total Glutathione ◽

Flower Extract ◽

Cox 2

Loranthus acaciae (Loranthaceae) is a perennial green semi-parasitic plant used in ethnopharmacological medicine for healing wounds. The protective effect of L. acaciae on gastric ulcer induced by ethanol was investigated in a rat model. Ulcer index and total glutathione level were measured and histological and immunohistochemical studies for the expression of cyclooxygenase-2 were performed. Furthermore, chemical constituents of the flower extract were analyzed. Ulcer index was significantly lowered in L. acaciae-treated groups. Protection ratios were 75.9%, 98.9%, and 70.7% for 250 mg/kg and 500 mg/kg of L. acaciae and 40 mg/kg of esomeprazole, respectively. Histological examination revealed fewer hemorrhage in mucosa and less edema in submucosa of L. acaciae-treated groups compared with control. In the esomeprazole-treated group, there was mild disruption in the surface epithelium and mild hemorrhage. However, edema and leucocytes infiltration in the submucosa layer were present. Immunohistochemical staining of stomach sections for cyclooxygenase-2 (COX-2) was negative in the control group as well as in the L. acaciae-treated groups. Total glutathione level in mucosa layer of the stomach was higher in L. acaciae-treated groups compared with control. Liquid chromatography-mass spectrometric analysis revealed the presence of loranthin and rutin as the major constituents. It can be concluded that L. acaciae imparted a gastroprotective action against ethanol-induced ulcer in rats. Novelty 500 mg/kg L. acaciae protected the stomach by 98.9% from ulcerogenic effect of ethanol. L. acaciae increased total glutathione level but not COX-2 expression in gastric mucosa. Loranthin and rutin were the major constituents in L. acaciae flower extract.

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