scholarly journals Association between KLOTHO Gene G395A Polymorphism and Carotid Artery Calcification in regular Hemodialysis Patients

2020 ◽  
Vol 8 (A) ◽  
pp. 208-213
Author(s):  
Herwindo Ahmad ◽  
Riri Andri Muzasti ◽  
Syafrizal Nasution
Keyword(s):  
Carotid Artery ◽  
Vascular Calcification ◽  
Vascular Dysfunction ◽  
Venous Blood ◽  
Hemodialysis Patients ◽  
Cross Sectional ◽  
Klotho Gene ◽  
Asian Populations ◽  
Carotid Artery Calcification ◽  
Regular Hemodialysis

BACKGROUND: The vascular calcification process in chronic kidney disease (CKD) is a complication caused by mineral and bone abnormalities and becomes the risk of cardiovascular disease and mortality in CKD patients. KLOTHO is an inhibitor of calcification and its expression has been observed to decrease in CKD patients. The KLOTHO gene G395A polymorphism is a genetic variation that is common in Asian populations and is associated with vascular dysfunction in hemodialysis patients. OBJECTIVE: The objective of the study was to determine the association between the KLOTHO gene G395A polymorphism and carotid artery calcification in regular hemodialysis patients. METHODS: This study was an analytical study with a cross-sectional design and was carried out at Rasyida Kidney Hospital Medan. Venous blood sample was taken from the patients who met the inclusion criteria for examination of the KLOTHO gene -395 polymorphism and carotid ultrasonography was assessed to evaluate the thickness of the tunica media-intima as a marker of vascular calcification. RESULTS: The majority of the study subjects were men, as many as 35 patients (50.7%). From the results of the KLOTHO gene -395 polymorphism, it was found that the majority of subjects had GG genotypes as many as 36 people (52.2%) followed by GA genotypes as many as 30 people (43,5%) and AA genotypes as many as 3 (4,3%). There was a statistically significant of the association between KLOTHO gene -395 polymorphism and the incidence of carotid artery calcification (p = 0,015). CONCLUSION: There was an association between the KLOTHO gene G395A polymorphism and carotid artery calcification in regular hemodialysis patients.

scholarly journals The Association Between Uric Acid and Symmetric Dimethylarginine Levels in the Patients Undergoing Twice-weekly Hemodialysis

2021 ◽  
Vol In Press (In Press) ◽  
Author(s):  
Aida Lydia ◽  
Yassir Yassir ◽  
Rudy Hidayat ◽  
Suhendro Suwarto
Keyword(s):  
Uric Acid ◽  
Venous Blood ◽  
Tertiary Hospital ◽  
Cross Sectional Study ◽  
Hemodialysis Patients ◽  
Bivariate Analysis ◽  
Symmetric Dimethylarginine ◽  
Cross Sectional ◽  
Increased Risk ◽  
History Of

Background: Uric acid (UA) levels are associated with increased risk of cardiovascular events and mortality in hemodialysis patients. However, there are still conflicting data on the mechanism of increased risks related to uric acid levels. Objectives: This study assessed the association between uric acid levels and symmetric dimethylarginine (SDMA), as a marker of cardiovascular disease, in the subjects undergoing hemodialysis twice weekly. Methods: This was a cross-sectional study conducted in a tertiary hospital in Jakarta, Indonesia. We included all the adults who underwent hemodialysis twice weekly for at least three months in our hospital. Subjects already on uric acid lowering therapy, pregnant or lactating women and those with a history of malignancy were excluded. Uric acid and SDMA levels were measured at the same time in pre-dialysis venous blood samples. Bivariate analysis was performed using the Mann-Whitney U test or one-way ANOVA. Results: A total of 126 subjects were included. The median level of UA was 8.4 mg/dL (IQR: 2.6, min: 4.1, max: 13.6), and 72 subjects (57.14%) had UA levels of 8 mg/dL or higher. The median SDMA level was 535.5 (312.7) mmol/dL (min: 119.7, max: 1895.5). Subjects with UA levels > 8 mg/dL had significantly higher SDMA levels compared to subjects with UA levels < 8 mg/dL (550.1 (IQR: 357.25) vs 491.35 (IQR: 181.1), P: 0.0475). Conclusions: In twice-weekly hemodialysis patients, UA levels above 8 mg/dL were associated with increased SDMA levels.

scholarly journals High Fetuin-A Level as a Protective Factor to Abdominal Aortic Calcification in Indonesian Regular Hemodialysis Patients

2019 ◽  
Vol 7 (5) ◽  
pp. 721-725 ◽  
Author(s):  
Riri Andri Muzasti ◽  
Ricke Loesnihari
Keyword(s):  
Vascular Calcification ◽  
Protective Factor ◽  
Hemodialysis Patients ◽  
Aortic Calcification ◽  
Enzyme Linked Immunosorbent Assay ◽  
Abdominal Aortic Calcification ◽  
Fetuin A ◽  
Inhibitory Factors ◽  
Abdominal Aortic ◽  
Regular Hemodialysis

BACKGROUND: Although the prevalence of cardiovascular disease decreases in the general population, this pattern is not followed in hemodialysis patients. Hence cardiovascular events still occur in 50% of cases resulting in hemodialysis patients. One of the risk factors is vascular calcification. The pathogenesis is not yet fully understood, but recent years studies have shown that vascular calcification in chronic kidney disease (CKD) occurs as a result of the interaction of stimulatory and inhibitory factors. One of the inhibitory factors is Fetuin-A. Until now there has been no data on levels of Fetuin-A as a risk factor for abdominal aortic calcification in Indonesia. AIM: To determine the effect of Fetuin-A levels on abdominal aortic calcification in regular hemodialysis patients. METHODS: This study was a cross-sectional study on 76 regular hemodialysis patients at Rasyida Renal Hospital Medan. Fetuin-A level was examined by enzyme-linked immunosorbent assay (ELISA). Assessment of abdominal aortic calcification was done by lateral lumbar X-ray. RESULTS: Most patients (68.4%) had abdominal aortic calcification, in both layers; intima and media (44.7%). Abdominal aortic calcification was associated with Fetuin-A level and age. Multivariate analysis showed that high Fetuin-A levels were significantly associated with abdominal aortic calcification. CONCLUSION: High Fetuin-A level appeared to be a protective factor against abdominal aortic calcification in regular hemodialysis patients in Indonesia.

Role of vascular calcification inhibitors in preventing vascular dysfunction and mortality in hemodialysis patients

2017 ◽  
Vol 31 (1) ◽  
pp. 72-81 ◽  
Author(s):  
Ozkan Gungor ◽  
Ismail Kocyigit ◽  
Mahmut Ilker Yilmaz ◽  
Siren Sezer
Keyword(s):  
Vascular Calcification ◽  
Vascular Dysfunction ◽  
Hemodialysis Patients ◽  
Calcification Inhibitors

Fetuin A and abdominal aorta calcification in hemodialysis patients

QJM ◽  
2021 ◽  
Vol 114 (Supplement_1) ◽  
Author(s):  
Osama Mahmoud Kamal ◽  
Tamer Waheed Elsaeed ◽  
Ashraf Hassan Abdel mobdy ◽  
Mohammed Abdel Halim Askar
Keyword(s):  
Kidney Disease ◽  
Vascular Calcification ◽  
Protective Factor ◽  
Calcium Score ◽  
Significant Negative Correlation ◽  
Hemodialysis Patients ◽  
High Serum ◽  
University Hospitals ◽  
Fetuin A ◽  
Regular Hemodialysis

Abstract Background Patients with chronic kidney disease (CKD), especially those on regular hemodialysis, have unique Mineral and endocrine disturbances. Vascular calcification is a dynamic process influenced by bone kidney disease, as well as an imbalance between promoters and inhibitors of extra-osseous bone formation. Calcification is defined as the accumulation of calcium salts in tissues. This is a specific type of mineralization, which in contrast to calcification involves the deposition of any mineral. Aim of the work To determine abdominal blood vessels calcification in hemodialysis patients and its relation to fetuin A plasma level. Patients and Methods This study included 60 patients (30 case and 30 control) admitted to Mansoura university hospitals. The patients were included in this study, all are End-stage renal disease (ESRD) patients on regular hemodialysis (HD) (4 Hours- 3 times weekly) for at least 3 month after obtaining informed conset from all patients). Results Our study showed non significant negative correlation between fetuin A and aortic calcium score . Conclusion High serum fetuin-A level was shown to be protective factor against vascular calcification and is associated with lower severity of abdominal aortic calcifications in regular hemodialysis patients.

scholarly journals LABORATORY FINDING AND RESTLESS LEGS SYNDROME IN REGULAR HEMODIALYSIS PATIENTS

2020 ◽  
Vol 7 (11) ◽  
pp. 68-71
Keyword(s):  
Binding Capacity ◽  
Laboratory Finding ◽  
Hemodialysis Patients ◽  
Baseline Characteristic ◽  
Restless Leg Syndrome ◽  
Laboratory Examination ◽  
Cross Sectional ◽  
Baseline Characteristics ◽  
Almost All ◽  
Regular Hemodialysis

Introduction: restless leg syndrome, a complication that increases morbidity and mortality in hemodialysis patients, is a concern. To explore RLS we can start by looking at the baseline characteristics of regular hemodialysis patients. Aim: To perform baseline characteristic and prevalence of RLS in regular hemodialysis patients. Metode: This study involved 71 regular hemodialysis patients at the Haji Adam Malik Medan General Hospital using a cross sectional design. Through interviews in accordance with the criteria of the International Restless Leg Syndrome Study Group (IRLSSG) RLS status is enforce. Data of laboratory finding were obtained by laboratory examination. Using the SPSS-21 application, the data has been analyzed. Result: Various baseline characteristics based on laboratory examination results (Hemoglobin; Calcium; Phosphate; Fibroblast Growth Factors-23; ferritin serum; Serum Iron; Tranferin Saturation; and Total Iron Binding Capacity) and prevalence of RLS in regular hemodialysis patients reach 26 respondents (36.6%) Conclusion: In regular hemodialysis patients, there is an increase in levels of phosphate, calcium and FGF-23. Almost all patients were anemic. Of 71 respondents, 26 respondents (36.6%) with RLS.

scholarly journals CROT (Carnitine O-Octanoyltransferase) Is a Novel Contributing Factor in Vascular Calcification via Promoting Fatty Acid Metabolism and Mitochondrial Dysfunction

2020 ◽  
Author(s):  
Takehito Okui ◽  
Masaya Iwashita ◽  
Maximillian A. Rogers ◽  
Arda Halu ◽  
Samantha K. Atkins ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Carotid Artery ◽  
Mitochondrial Dysfunction ◽  
Vascular Calcification ◽  
Fatty Acid Metabolism ◽  
Acid Metabolism ◽  
Plasma Cholesterol ◽  
Human Coronary Artery ◽  
Carotid Artery Calcification ◽  
Collagen Secretion

Objective: Vascular calcification is a critical pathology associated with increased cardiovascular event risk, but there are no Food and Drug Administration-approved anticalcific therapies. We hypothesized and validated that an unbiased screening approach would identify novel mediators of human vascular calcification. Approach and Results: We performed an unbiased quantitative proteomics and pathway network analysis that identified increased CROT (carnitine O-octanoyltransferase) in calcifying primary human coronary artery smooth muscle cells (SMCs). Additionally, human carotid artery atherosclerotic plaques contained increased immunoreactive CROT near calcified regions. CROT siRNA reduced fibrocalcific response in calcifying SMCs. In agreement, histidine 327 to alanine point mutation inactivated human CROT fatty acid metabolism enzymatic activity and suppressed SMC calcification. CROT siRNA suppressed type 1 collagen secretion, and restored mitochondrial proteome alterations, and suppressed mitochondrial fragmentation in calcifying SMCs. Lipidomics analysis of SMCs incubated with CROT siRNA revealed increased eicosapentaenoic acid, a vascular calcification inhibitor. CRISPR/Cas9-mediated Crot deficiency in LDL (low-density lipoprotein) receptor-deficient mice reduced aortic and carotid artery calcification without altering bone density or liver and plasma cholesterol and triglyceride concentrations. Conclusions: CROT is a novel contributing factor in vascular calcification via promoting fatty acid metabolism and mitochondrial dysfunction, as such CROT inhibition has strong potential as an antifibrocalcific therapy.

scholarly journals Correlation of Plasma Klotho Levels and Carotid Intima- Media Thickness in CKD Patients on Regular Hemodialysis

2019 ◽  
Vol 2 (3) ◽  
Keyword(s):  
Risk Factors ◽  
Chronic Kidney Disease ◽  
Logistic Regression ◽  
Kidney Disease ◽  
Carotid Artery ◽  
Vascular Calcification ◽  
Plasma Levels ◽  
Pearson Correlation ◽  
Intima Media Thickness ◽  
Regular Hemodialysis

Background: The incidence of heart death is 5-10 times more in patients with chronic kidney disease (CKD) with regular hemodialysis compared to the general population. Vascular calcification is a risk factor. In CKD patients there is a decrease in the distal renal cell cells which are the main producer of Klotho in the body. The functions of Klotho are maintaining integrity, endothelial permeability and endogenous inhibitors of vascular calcification. Previous studies reported an association between vascular calcification and Klotho plasma levels. Vascular calcification for heart disease can be determined by the thickness Intima Media (TIM) Carotid Artery. Objective: To determine the correlation of plasma Klotho levels with the Intima Media thickness of the Carotid Artery in CKD patients with regular hemodialysis. Methode: This cross-sectional study was conducted at the Rasyida Kidney General Hospital in Medan from April to July 2018. Patients with CKD who have undergone routine hemodialysis are examined for plasma levels of Klotho and real time ultrasound examinations to determine the thickness of arterial intima media carotid. Data are analyzed with Mann Whitney test, binominal logistic regression and Pearson correlation. Results: Seventy patients with CKD showed an average plasma Klotho level of 281.43 + 298.63 pg / ml with an average TIM of the carotid artery of 0.22 + 0.09 cm. Patients were divided into 2 groups, namely the calcification group (n = 39) and the noncalcified group (n = 31). Mann-Whitney test was performed with the results that there were differences in plasma Klotho levels between the calcification group and the noncalcified carotid artery group which were statistically significant (p = 0.001). The binominal logistic regression analysis test was performed on risk factors associated with the incidence of vascular calcification and the results of Klotho serum levels, Diabetes Mellitus and Hypertension were found to be significant risk factors for calcification (p <0.05). With the Pearson correlation test, the negative correlation of plasma Klotho levels with the thickening of Intima Carotid Arterial Media was statistically significant with moderate correlation strength (p = 0.002; r = -0.368). Conclusion: Patients with chronic kidney disease with low plasma Klotho levels have thickening of the intima media of carotid artery.

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