scholarly journals Immunohistochemical Study of IMP3 Expression in Laryngeal Squamous Cell Carcinoma

2021 ◽  
Vol 9 (A) ◽  
pp. 1168-1173
Author(s):  
Wala’a Ahmad Al-Sayed Ashmawy ◽  
Ahmed Mahmoud Abd-Elaziz ◽  
Amira Mohamed Bassam ◽  
Heba Abdelmonem Ibrahim
Keyword(s):  
Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Tumor Tissue ◽  
Rna Binding ◽  
Laryngeal Squamous Cell Carcinoma ◽  
Malignant Tumors ◽  
Hematoxylin And Eosin ◽  
Department Faculty

BACKGROUND: IMP3 is an RNA binding protein, which is strongly expressed in malignant tumors, promoting tumor cell proliferation. AIM: The aim of the study was to evaluate the expression of IMP3 in laryngeal squamous cell carcinoma (SCC) and to correlate the expression of IMP3 with available clinicopathological data. METHODS: Sixty one total laryngectomy and laryngoscopic biopsies; collected from the Pathology Department, Faculty of Medicine, Cairo University. Two slides were prepared from each paraffin embedded tumor block, one slide for Hematoxylin and Eosin staining, and the other for immunohistochemical staining by IMP3 polyclonal antibody. RESULTS: Thirty-seven cases (60.7%) showed positive IMP3 expression, and a statistically significant correlation was found between IMP3 expressions in normal, dysplastic epithelium/in situ component, and the invasive malignant tumor tissue. Correlations between IMP3 expression and other available clinicopathological data were all non-significant. CONCLUSION: This study suggests that IMP3 might play a role in laryngeal SCC carcinogenesis and progression process from normal to dysplastic to malignant epithelium, and thus IMP3 might be targeted by gene therapy.

scholarly journals Metastatic Squamous Cell Carcinoma of the Anus to the Lung Confirmed with Allelotyping

2014 ◽  
Vol 2014 ◽  
pp. 1-5 ◽  
Author(s):  
Rachel Roth ◽  
Susan Moffatt-Bruce ◽  
Marino E. Leon
Keyword(s):  
Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Loss Of Heterozygosity ◽  
Squamous Cell ◽  
Palliative Chemotherapy ◽  
Primary Tumors ◽  
Appropriate Treatment ◽  
Metastatic Squamous Cell Carcinoma ◽  
Hematoxylin And Eosin

Histopathologic techniques are insufficient for distinguishing primary squamous cell carcinoma (SCC) from metastatic SCC, which is clinically important. A patient with SCC of the anus was found to also have SCC of the lung, and the question of metastatic versus synchronous primary diseases was raised. Immunohistochemical and hematoxylin and eosin (H&E) staining on sections of tissue could not discriminate between the two entities. Immunostain for p16 and chromogenicin situhybridization for human papillomavirus (HPV) type 16 were positive in both tumors. Additionally, allelotyping for loss of heterozygosity displayed similar findings and confirmed the histopathological impression of anal SCC metastasis to the lung. The patient was treated with palliative chemotherapy instead of additional surgical treatment. When multiple tumors are present, determining metastatic versus synchronous primary tumors is necessary for appropriate treatment. Identification can be achieved using allelotyping for loss of heterozygosity.

scholarly journals NFIB-Mediated lncRNA PVT1 Aggravates Laryngeal Squamous Cell Carcinoma Progression via the miR-1301-3p/MBNL1 Axis

2021 ◽  
Vol 2021 ◽  
pp. 1-17
Author(s):  
Tian Tang ◽  
Feng Zeng
Keyword(s):  
Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Molecular Mechanisms ◽  
Laryngeal Squamous Cell Carcinoma ◽  
Malignant Tumors ◽  
Basic Research ◽  
World Health ◽  
The Past ◽  
Therapy Strategies

Laryngeal squamous cell carcinoma (LSCC) is one of the most common malignant tumors of head and neck cancers. In the past decades, although the therapy strategies of LSCC have made considerable improvement, the terrible outcomes of LSCC still bring an enormous burden to the world health care system. Novel therapeutic targets for LSCC are urgently needed. lncRNAs exert important roles in various biological progressions, including LSCC. Here, we aimed to investigate the function of lncRNA PVT1 in LSCC progression and its underlying molecular mechanisms. By conducting multiple experiments, our results showed that lncRNA PVT1 was upregulated in LSCC cell lines and regulated LSCC cell proliferation, apoptosis, and its cell susceptibility to natural killer (NK) cells. Moreover, it was found that lncRNA PVT1 promotes MBNL1 expression to regulate LSCC cellular progression through sponging miR-1301-3p. Our study might provide novel targets for LSCC basic research or clinical management.

scholarly journals The Nuclear Localization of NFκB and p53 Is Positively Correlated with HPV16 E7 Level in Laryngeal Squamous Cell Carcinoma

2003 ◽  
Vol 51 (4) ◽  
pp. 533-539 ◽  
Author(s):  
Jing Du ◽  
George G. Chen ◽  
Alexander C. Vlantis ◽  
Hu Xu ◽  
Raymond K.Y. Tsang ◽  
...  
Keyword(s):  
Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Nuclear Localization ◽  
Laryngeal Cancer ◽  
Squamous Cell ◽  
Tumor Tissue ◽  
Laryngeal Squamous Cell Carcinoma ◽  
Tumor Tissues ◽  
A Subunit ◽  
Human Papilloma Virus 16

The interaction between the HPV (human papilloma virus) 16 E7 and other cell growth factors, such as p53 and NFκB in laryngeal cancer is not clearly understood. The aim of this study was to examine the expression of these three proteins in tumor and non-tumor laryngeal tissues from patients with laryngeal squamous cell carcinoma. These three proteins were dominantly expressed in the nucleus and their levels were higher in the tumor tissue than in the non-tumor tissue, although the comparison between the tumor and non-tumor tissues of p53 staining did not reach significance. The intensity of the nuclear stain of E7 and p53 was stronger than that of p65, a subunit of NFκB. Correlation analysis revealed that there was a positive relationship between the level of HPV16 E7 and the expression of p65. The correlation between E7 and p53 was also significant, although to a lesser degree. The finding of nuclear localization of p65 suggests that NFκB is constantly activated in the laryngeal cancer cells, whereas the sequestration of p53 in the nucleus may represent a mutated form of p53, which is probably inactivated by HPV16 oncoproteins. In conclusion, this study suggests that the nuclear localization of NFκB and p53 may play a role in the development of human laryngeal squamous cell carcinoma infected with HPV16.

scholarly journals CTCF-silenced miR-137 contributes to EMT and radioresistance in esophageal squamous cell carcinoma

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Shuwen Xu ◽  
Xiaofeng Li ◽  
Longfei Li ◽  
Yufeng Wang ◽  
Chong Geng ◽  
...  
Keyword(s):  
Squamous Cell Carcinoma ◽  
Esophageal Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Rna Binding ◽  
Epithelial Mesenchymal Transition ◽  
Malignant Tumors ◽  
Luciferase Reporter ◽  
Mesenchymal Transition ◽  
Polycomb Repressive Complex 2

Abstract Background Esophageal squamous cell carcinoma (ESCC) is one of the most malignant tumors in gastrointestinal system. MicroRNAs (miRNAs) have been reported to be implicated in cancer development. However, the role of miR-137 has not been fully revealed in ESCC. Methods Quantitative real-time polymerase chain reaction (qRT-PCR) and western blot analyses were separately used to examine RNA level and protein level. 5-ethynyl-2′-deoxyuridine (EdU) assay, transwell assays and flow cytometry analyses were conducted to assess biological behaviors of ESCC cells. Additionally, the interaction between genes were analyzed via Chromatin Immunoprecipitation (ChIP) assay, RNA Binding Protein Immunoprecipitation (RIP) assay, RNA pull down assay and luciferase reporter assay. Results MiR-137 was down-regulated in ESCC cells. Upregulation of miR-137 hindered ESCC cell proliferation, migration, invasion and epithelial mesenchymal transition (EMT). Besides, miR-137 enhanced the sensitivity of ESCC cells to irradiation. Moreover, CCCTC-binding factor (CTCF) inactivated miR-137 transcription in ESCC cells. Furthermore, we revealed enhancer of zeste 2 polycomb repressive complex 2 subunit (EZH2) and paxillin (PXN) as the downstream targets of miR-137. In turn, EZH2 was recruited by CTCF and induced methylation in miR-137 promoter. Conclusion CTCF/Suz12/EZH2 complex-silenced miR-137 facilitates ESCC progression and radioresistance by targeting EZH2 and PXN.

Heterogeneity of 11q13 region rearrangements in laryngeal squamous cell carcinoma analyzed by microarray platforms and fluorescence in situ hybridization

2013 ◽  
Vol 40 (7) ◽  
pp. 4161-4171 ◽  
Author(s):  
Małgorzata Jarmuz-Szymczak ◽  
Kinga Pelinska ◽  
Magdalena Kostrzewska-Poczekaj ◽  
Ewa Bembnista ◽  
Maciej Giefing ◽  
...  
Keyword(s):  
Squamous Cell Carcinoma ◽  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Laryngeal Squamous Cell Carcinoma
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