Differentially expressed protein markers in human submandibular and sublingual secretions

2004 ◽  
Author(s):  
Shen Hu ◽  
Patricia Denny ◽  
Paul Denny ◽  
Yongming Xie ◽  
Joseph Loo ◽  
...  
Keyword(s):  
Differentially Expressed ◽  
Protein Markers ◽  
Differentially Expressed Protein

Application of proteomics for the identification of differentially expressed protein markers for Down syndrome in maternal plasma

2008 ◽  
Vol 28 (8) ◽  
pp. 691-698 ◽  
Author(s):  
Aggeliki Kolialexi ◽  
George Th. Tsangaris ◽  
Nikos Papantoniou ◽  
Athanasios K. Anagnostopoulos ◽  
Kostantinos Vougas ◽  
...  
Keyword(s):  
Down Syndrome ◽  
Maternal Plasma ◽  
Differentially Expressed ◽  
Protein Markers ◽  
Differentially Expressed Protein

scholarly journals The Alterations in Methylene Blue/Light-Treated Frozen Plasma Proteins Revealed by Proteomics

2021 ◽  
pp. 1-8
Author(s):  
Tiange Wu ◽  
Xiaoning Wang ◽  
Kai Ren ◽  
Xiaochen Huang ◽  
Jiankai Liu
Keyword(s):  
Mass Spectrometry ◽  
Methylene Blue ◽  
Western Blot ◽  
Blue Light ◽  
Differentially Expressed ◽  
Enzyme Linked Immunosorbent Assay ◽  
Differentially Expressed Protein ◽  
Significant Difference ◽  
Modified Proteins ◽  
Frozen Plasma

Introduction: The aim of this study was to investigate the modified proteins in methylene blue/light-treated frozen plasma (MB-FP) compared with fresh frozen plasma (FFP) in order to gain a better application of MB/light-treated plasma in clinic transfusion. Methods: MB-FP and FFP were collected from Changchun central blood station, and a trichloroacetic acid/acetone precipitation method was used to remove albumin for the enrichment of lower abundance proteins. The plasma protein in MB-FP and FFP were separated using two-dimensional gel electrophoresis (2-DE) and the differentially expressed protein spots were analyzed using mass spectrometry. Finally, the differentially expressed proteins were tested using Western blot and enzyme-linked immunosorbent assay (ELISA). Results: Approximately 14 differentially expressed protein spots were detected in the MB-FP, and FFP was chosen as the control. After 2-DE comparison analysis and mass spectrometry, 8 significantly differentially expressed protein spots were identified, corresponding to 6 different proteins, including complement C1r subcomponent (C1R), inter-alpha-trypsin inhibitor heavy chain H4 (ITI-H4), keratin, type II cytoskeletal 1 (KRT1), hemopexin (HPX), fibrinogen gamma chain (FGG), and transthyretin (TTR). Western blot showed no significant difference in the expression level of KRT1 between MB-FP and FFP (p > 0.05). Both Western blot and ELISA indicated that the level of HPX was significantly higher in FFP than in MB-FP (p < 0.05). Conclusion: This comparative proteomics study revealed that some significantly modified proteins occur in MB-FP, such as C1R, ITI-H4, KRT1, HPX, FGG, and TTR. Our findings provide more theoretical data for using MB-FP in transfusion medicine. However, the relevance of the data for the transfusion of methylene blue/light-treated plasma remains unclear. The exact modification of these proteins and the effects of these modified proteins on their functions and their effects in clinical plasma infusion need to be further studied.

Proteomic analysis in clear cell renal cell carcinoma: identification of differentially expressed protein by 2-D DIGE

2012 ◽  
Vol 8 (4) ◽  
pp. 1040 ◽  
Author(s):  
Francesca Raimondo ◽  
Claudia Salemi ◽  
Clizia Chinello ◽  
Daniela Fumagalli ◽  
Lavinia Morosi ◽  
...  
Keyword(s):  
Renal Cell Carcinoma ◽  
Cell Carcinoma ◽  
Proteomic Analysis ◽  
Renal Cell ◽  
Clear Cell ◽  
Differentially Expressed ◽  
Cell Renal Cell Carcinoma ◽  
Differentially Expressed Protein

Identification of One Polysaccharide Gene and Analysis of Differentially Expressed Proteins from Bioflocculant Producing Strain of F2

2010 ◽  
Vol 113-116 ◽  
pp. 305-310
Author(s):  
Bing Yu ◽  
Fang Ma ◽  
Pei Shi Qi ◽  
Ang Li ◽  
Li Li Wang
Keyword(s):  
Protein Complexes ◽  
Relative Content ◽  
Differentially Expressed ◽  
Chain Reaction ◽  
The Core ◽  
Differentially Expressed Protein ◽  
Sds Page ◽  
Flocculating Activity ◽  
Polymerase Chain ◽  
Minimal Media

F2 is one of two compound bioflocculant producing strains with good genetic stability and high flocculating activity. The core sequence of exoL was amplified from the F2 genome by the method of polymerase chain reaction, identifying that the effective polysaccharide component of bioflocculant produced by F2 was succinoglycan. The changes of protein complexes of F2 grown in minimal media versus bioflocculant media were researched by SDS-PAGE, indicating that in bioflocculant media compared with minimal media, the relative content of two protein complexes were obviously increased and the relative content of one protein complexes was obviously decreased in gels. It was three differentially expressed protein complexes that can probably induce the production of large amount of the effective polysaccharide component of bioflocculant produced by F2. The research can provide a step to reveal the molecular flocculating mechanisms of compound bioflocculant producing strains.

scholarly journals Comprehensive Analysis Reveals the Potential Regulatory Mechanism Between Ub–Proteasome System and Cell Cycle in Colorectal Cancer

2021 ◽  
Vol 9 ◽  
Author(s):  
Zhiyuan Zhang ◽  
Jingwen Chen ◽  
Wentao Tang ◽  
Qingyang Feng ◽  
Jianmin Xu ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Cell Cycle ◽  
Regulatory Mechanism ◽  
Differentially Expressed ◽  
Potential Mechanism ◽  
Ppi Network ◽  
Protein Coding ◽  
Protein Protein Interaction ◽  
Differentially Expressed Protein ◽  
Cycle System

The ubiquitin (Ub)–proteasome system (UPS) is an important regulatory component in colorectal cancer (CRC), and the cell cycle is also characterized to play a significant role in CRC. In this present study, we firstly identified UPS-associated differentially expressed genes and all the differentially expressed protein-coding genes in CRC through three differential analyses. UPS-associated genes were also further analyzed via survival analysis. A weighted gene co-expression network analysis (WGCNA) was used to identify the cell cycle-associated genes. We used protein–protein interaction (PPI) network to comprehensively mine the potential mechanism of the UPS–cell cycle regulatory axis. Moreover, we constructed a signature based on UPS-associated genes to predict the overall survival of CRC patients. Our research provides a novel insight view of the UPS and cell cycle system in CRC.

scholarly journals Comparative proteome analysis of the spinal dural arteriovenous fistula arterial draining vein with label-free quantitative proteomics

2020 ◽  
Author(s):  
Peixi Liu ◽  
Yuan Shi ◽  
Sichen Li ◽  
Yingjun Liu ◽  
Yingjie Zhou ◽  
...  
Keyword(s):  
Quantitative Proteomics ◽  
Dural Arteriovenous Fistula ◽  
Kegg Pathway ◽  
Differentially Expressed ◽  
Differentially Expressed Proteins ◽  
Label Free ◽  
Spinal Dural Arteriovenous Fistula ◽  
Differentially Expressed Protein ◽  
Kegg Pathway Analysis ◽  
Go Terms

Abstract Background: Spinal dural arteriovenous fistula (SDAVF) is the most common spinal vascular shunt lesion. Although pathological changes in the SDAVF draining vein (SDAVF-DV) have been elucidated, protein changes remain enigmatic. We investigated protein changes in the SDAVF-DV.Methods: Three SDAVF-DV samples were collected, and superficial temporal artery (STA) and superficial temporal vein (STV) samples were used as controls. After quantification and enzymolysis of the proteins, label-free quantitative proteomics was performed, and the peptide mixture was fractionated and analysed by liquid chromatography tandem mass spectrometry (LC-MS/MS) to identify the differentially expressed proteins. Bioinformatics analysis of the differentially expressed proteins was also performed using Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG) and protein-protein interaction (PPI) network analyses.Results: Compared with the STA, the SDAVF-DV had 195 upregulated proteins and 303 downregulated proteins. GO analysis showed that the most differential GO terms in each category were the adenylate cyclase-modulating G protein-coupled receptor signalling pathway, U6 snRNP and SH3 domain binding. KEGG pathway analysis showed that the most differentially expressed protein pathway was focal adhesion. Compared with the STV, the SDAVF-DV had 158 upregulated proteins and 362 downregulated proteins. GO analysis showed that the most differential GO terms in each category were lamellipodium assembly, U6 snRNP, and SH3 domain binding. KEGG pathway analysis showed that the most differentially expressed protein pathway was dilated cardiomyopathy. The PPI analysis revealed PPIs among the top 300 proteins.Conclusions: We demonstrated that the SDAVF-DV showed specific protein expression changes under long-period venous hypertension. The results of the present study will provide insights into the pathogenesis of SDAVF formation at the protein level. The proteomic results provide a scientific foundation for further study to explore the pathophysiological mechanism of SDAVF.

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