Tanshinone IIA enhances the inhibitory effect of imatinib on proliferation and motility of acute leukemia cell line TIB‑152 in�vivo and in�vitro by inhibiting the PI3K/AKT/mTOR signaling pathway

Background & Purpose: In evaluating new drugs for the treatment of various types of cancer, investigations have been made to discover a variety of anti-tumor compounds with less side effects on normal cells. Investigations have shown that the heterodimers S100A8 and S100A9 inhibit the enzyme casein kinase 2 and then prevent the activation of the E7 oncoprotein. Therefore, the aim of this study was to evaluate the effect of calprotectin as an antitumor compound on the Nalm6 (B cell precursor leukemia cell line). Material & Methods: Transformation of genes encoding S100A8 and S100A9 human, designed in the pQE32 plasmid, was performed by the thermal shock method into E. coli M15 bacteria. After bacterial growth in LB medium, the expression of two S100A8 and S100A9 subunits, the solubility of the protein by SDS-PAGE method was determined. Finally, the S100A8 / A9 complex was equally placed in the microtube. In the next step, the cytotoxic effects of calprotectin produced on the Nalm6 cell line were evaluated using the wst1 test. Then, the apoptosis in these cells was measured using flow cytometry methods with Annexin-V coloration. Results: In the current study, the results showed that the cytotoxic effects of Calprotectin are time and concentration- dependent. Therefore, it can reduce the tumor expression and had a beneficial effect by induced apoptosis in Nalm6 cell line. Conclusion: Calprotectin has an anti-tumor effect on the Nalm6 cell line by increasing apoptosis.

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Modulation of Human Basophil Growth In Vitro by Xiao-Qing-Long-Tang (Syo-seiryu-to), Chai-Pu-Tang (Saiboku-to), Qing-Fei-Tang (Seihai-to), Baicalein and Ketotifen

The American Journal of Chinese Medicine ◽

10.1142/s0192415x89000085 ◽

1989 ◽

Vol 17 (01n02) ◽

pp. 45-50 ◽

Cited By ~ 10

Author(s):

Yasuo Tanno ◽

Yuriko Shindoh ◽

Tamotsu Takishima

Keyword(s):

Bronchial Asthma ◽

Mononuclear Cells ◽

Leukemia Cell ◽

Allergic Diseases ◽

Inhibitory Effect ◽

Human Basophil ◽

Leukemia Cell Line ◽

Dependent Manner

Our previous study showed the inhibitory effect of Qing-Fei-Tang (Q.T.F.) and baicalein on the leukotriene (LT)B4 synthesis of human alveolar macrophages. It has recently been demonstrated that LTs support various cell growth, and basophil and its precursor numbers increase in atopic patients. Therefore, we examined the effect of anti-allergic drugs, including Q.F.T., Xiao-Qing-Long-Tang (X.Q.L.T.), Chai-Pu-Tang (C.P.T.), baicalein and ketotifen which have been used for treatment of bronchial asthma, on human basophil growth in vitro using cord blood mononuclear cells as a basophil precursor source and conditioned medium of T cell leukemia cell line Mo as a growth factor. Two-week cultured basophil numbers identified by alcian blue-safranin staining and those histamine contents assayed fluorometrically were inhibited by Q.F.T. (1.0 mg/ml), X.Q.L.T. (0.01–1.0 mg/ml), C.P.T. (0.01–1.0 mg/ml), baicalein (1–100 μM) or ketotifen (1–100 μM) in a dose-dependent manner while low dose (0.01–0.1 mg/ml) of Q.F.T. showed an enhancing effect on the basophil growth and the histamine content. However, LTB4 or LTC4 failed in restoring the basophil growth reduced by 1 mg/ml of C.P.T. or 100 μM of ketotifen. These results suggest that anti-allergic drugs may modulate basophil growth and differentiation in vitro and/or in vivo and therefore by useful and reasonable for controlling allergic diseases including bronchial asthma.

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MUTZ-3, a Human Cell Line Modell for Osteoclast Differenziation.

Blood ◽

10.1182/blood.v104.11.4133.4133 ◽

2004 ◽

Vol 104 (11) ◽

pp. 4133-4133

Author(s):

Anna C. Berardi ◽

Antonina Parafioriti ◽

Ginfranco Bottazzo ◽

Elisa Ciraci

Keyword(s):

Cell Line ◽

Myeloid Leukemia ◽

Osteoclast Differentiation ◽

Leukemia Cell ◽

Human Cell Line ◽

Leukemia Cell Line ◽

Cell Phenotype ◽

Human Cd34

Abstract No human myeloid leukemia-derived cell lines possess the ability to acquire a osteoclstic cell phenotype. In contrast, the CD34+ human acute myeloid leukemia cell line MUTZ-3 responds to macrophage-colony-stimulating-factor (M-CSF), receptor activator NF-kB ligand (RANK-L) and tumor necrosis factor-a (TNF-a), cytokines known to be pivotal both in vivo and in vitro for osteoclasts generation from monocytes and CD34+ stem cells. In all respects, MUTZ-3 cells behave as the immortalized equivalent of CD34+ osteoclasts precursors. Upon stimulation with specific cytokine cocktails, they formed numerous multinucleated osteoclasts expressing tartrate resistent acid phosphatase (TRAP) and calcitonin receptors (CTR) within 12 days of culture. MUTZ-3-osteoclasts formed extensive lacunae mineral resorption when cultured on mineralized surface after 21 day of cultures. These findings demonstrate the unique suitability of MUTZ-3 cells as an unlimited source of human CD34+ osteoclasts progenitors for the study of cytokine-induced osteoclast differentiation.

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L-securinine induces apoptosis in the human promyelocytic leukemia cell line HL-60 and influences the expression of genes involved in the PI3K/AKT/mTOR signaling pathway

Oncology Reports ◽

10.3892/or.2014.3101 ◽

2014 ◽

Vol 31 (5) ◽

pp. 2245-2251 ◽

Cited By ~ 12

Author(s):

SHUWEN HAN ◽

GANG ZHANG ◽

MAIDONG LI ◽

DONGYUN CHEN ◽

YING WANG ◽

...

Keyword(s):

Cell Line ◽

Signaling Pathway ◽

Leukemia Cell ◽

Promyelocytic Leukemia ◽

Mtor Signaling ◽

Leukemia Cell Line ◽

Mtor Signaling Pathway ◽

Human Promyelocytic Leukemia Cell ◽

Expression Of Genes ◽

Promyelocytic Leukemia Cell Line

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Upregulation of Long Noncoding RNA_GAS5 Suppresses Cell Proliferation and Metastasis in Laryngeal Cancer via Regulating PI3K/AKT/mTOR Signaling Pathway

Technology in Cancer Research & Treatment ◽

10.1177/1533033821990074 ◽

2021 ◽

Vol 20 ◽

pp. 153303382199007

Author(s):

Wenlin Liu ◽

Jiandong Zhan ◽

Rong Zhong ◽

Rui Li ◽

Xiaoli Sheng ◽

...

Keyword(s):

Cell Proliferation ◽

Signaling Pathway ◽

Laryngeal Cancer ◽

Mtor Signaling ◽

Mtor Signaling Pathway ◽

Proliferation And Metastasis ◽

Cancer Tissues ◽

Lncrna Gas5

Background: Laryngeal cancer is one of the most common malignant tumors among head and neck cancers. Accumulating studies have indicated that long noncoding RNAs (lncRNAs) play an important role in laryngeal cancer occurrence and progression, however, the functional roles and relative regulatory mechanisms of lncRNA growth arrest-specific transcript 5 (GAS5) in laryngeal cancer progression remain unclear. Methods: The expression of lncRNA GAS5 in both laryngeal cancer tissues and cell lines was evaluated using quantitative reverse transcription-polymerase chain reaction (RT-qPCR) assay. The relationships between lncRNA GAS5 expression and clinical parameters were also analyzed. To determine the biological function of lncRNA GAS5, a lncRNA GAS5-specific plasmid was first transfected into laryngeal cancer cells using lentiviral technology. Cell counting kit-8 assay, flow cytometry, and Transwell assays were used to detect in vitro cell proliferation, apoptosis, cycle distribution, and metastasis abilities, respectively. Furthermore, in vivo cell growth experiments were also performed using nude mice. Additionally, western blotting was performed to identify the underlying regulatory mechanism. Results: In the current study, lncRNA GAS5 was downregulated in laryngeal cancer tissues and its low expression was closely associated with poor tumor differentiation, advanced TNM stage, lymph node metastasis, and shorter overall survival time. In addition, lncRNA GAS5 upregulation significantly inhibited laryngeal cancer cell proliferation both in vitro and in vivo. Moreover, in response to lncRNA GAS5 overexpression, more laryngeal cancer cells were arrested at the G2/M stage, accompanied by increased cell apoptosis rates and suppressed migration and invasion capacities. Mechanistically, our data showed that the overexpression of lncRNA GAS5 significantly regulated the PI3K/AKT/mTOR signaling pathway. Conclusion: LncRNA GAS5 might act as a suppressor gene during laryngeal cancer development, as it suppressed cell proliferation and metastasis by regulating the PI3K/AKT/mTOR signaling pathway; thus, lncRNA GAS5 is a promising therapeutic biomarker for the treatment of laryngeal cancer.

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The Human Leukemic HL-60 Cell Line: An in Vitro Model for Cell Death Endpoint Identification

Alternatives to Laboratory Animals ◽

10.1177/026119299602400419 ◽

1996 ◽

Vol 24 (4) ◽

pp. 581-587

Author(s):

Cristiana Zanetti ◽

Arrnalaura Stammati ◽

Orazio Sapora ◽

Flavia Zucco

Keyword(s):

Cell Death ◽

Cell Line ◽

In Vitro Model ◽

Leukemia Cell ◽

Promyelocytic Leukemia ◽

Leukemia Cell Line ◽

Cell Type ◽

Vitro Model ◽

Promyelocytic Leukemia Cell Line

The aim of this study was to investigate the endpoints related to cell death, either necrosis or apoptosis, induced by four chemicals in the promyelocytic leukemia cell line, HL-60. Cell morphology, DNA fragmentation, cytofluorimetric analysis and oxygen consumption were used to classify the type of cell death observed. In our analysis, we found that not all the selected parameters reproduced the differences observed in the cell death caused by the four chemicals tested. As cell death is a very complex phenomenon, several factors should be taken into account (cell type, exposure time and chemical concentration), if chemicals are to be classified according to differences in the mechanisms more directly involved in cell death.

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Human acute leukemia cell line with the t(4;11) chromosomal rearrangement exhibits B lineage and monocytic characteristics

Blood ◽

10.1182/blood.v65.1.21.21 ◽

1985 ◽

Vol 65 (1) ◽

pp. 21-31 ◽

Cited By ~ 194

Author(s):

RC Stong ◽

SJ Korsmeyer ◽

JL Parkin ◽

DC Arthur ◽

JH Kersey

Keyword(s):

Acute Leukemia ◽

Cell Line ◽

Lymphoblastic Leukemia ◽

Leukemia Cell ◽

Leukemic Cells ◽

Leukemia Cell Line ◽

Terminal Deoxynucleotidyl Transferase ◽

Immunoglobulin Gene ◽

A Cell ◽

B Lineage

Abstract A cell line, designated RS4;11, was established from the bone marrow of a patient in relapse with an acute leukemia that was characterized by the t(4;11) chromosomal abnormality. The cell line and the patient's fresh leukemic cells both had the t(4;11)(q21;q23) and an isochromosome for the long arm of No. 7. Morphologically, all cells were lymphoid in appearance. Ultrastructurally and cytochemically, approximately 30% of the cells possessed myeloid features. The cells were strongly positive for terminal deoxynucleotidyl transferase. They were HLA-DR positive and expressed surface antigens characteristic for B lineage cells, including those detected by anti-B4, BA-1, BA-2, and PI153/3. Immunoglobulin gene analysis revealed rearrangements of the heavy chain and kappa chain genes. The cells lacked the common acute lymphoblastic leukemia antigen and antigenic markers characteristic of T lineage cells. The cells reacted with the myeloid antibody 1G10 but not with other myeloid monoclonal antibodies. Treatment with 12-O-tetradecanoyl- phorbol-13-acetate induced a monocyte-like phenotype demonstrated by cytochemical, functional, immunologic, and electron microscopic studies. The expression of markers of both early lymphoid and early myeloid cells represents an unusual phenotype and suggests that RS4;11 represents a cell with dual lineage capabilities. To our knowledge, RS4;11 is the first cell line established from t(4;11)-associated acute leukemia.

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Human Platelets Effectively Kill K-562 Cells, a Chronic Myelogenic Leukemia Cell Line,in vitro

Japanese Journal of Cancer Research ◽

10.1111/j.1349-7006.1990.tb02590.x ◽

1990 ◽

Vol 81 (5) ◽

pp. 449-453 ◽

Cited By ~ 8

Author(s):

Terutaka Sagawa ◽

Takeshi Kodama ◽

Akio Tominaga ◽

Mariko Okada

Keyword(s):

Cell Line ◽

Leukemia Cell ◽

Human Platelets ◽

Leukemia Cell Line ◽

K 562 Cells

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MO243STUDY ON THE EFFECT AND MECHANISM OF NOVEL REGULATORY T CELL (CD4+CD126LOWFOXP3+ TREG) IMMUNOTHERAPY IN LUPUS NEPHRITIS*

Nephrology Dialysis Transplantation ◽

10.1093/ndt/gfab104.001 ◽

2021 ◽

Vol 36 (Supplement_1) ◽

Author(s):

Zhenjian Xu ◽

Junzhe Chen ◽

Anping Xu

Keyword(s):

T Cell ◽

Lupus Nephritis ◽

Signaling Pathway ◽

B Lymphocytes ◽

Mtor Signaling ◽

Mtor Signaling Pathway ◽

Proliferation And Differentiation ◽

Inflammatory State

Abstract Background and Aims Our previous study found a new regulatory T cell subpopulation, CD4+CD126lowFoxp3+ regulatory T cells (CD4+CD126lowFoxp3+ Treg). This cell can maintain a stable immune regulatory function in the inflammatory state. Through in vivo and in vitro experiments, we have confirmed that CD4+CD126lowFoxp3+ Treg has an immunotherapeutic effect on T cell-mediated mouse models of autoimmune diseases such as colitis and collagen-induced arthritis (CIA). Further experimental studies showed that CD4+CD126lowFoxp3+ Treg could reduce the kidney injury caused by autoantibodies and prolong the survival time of lupus mice. However, the mechanism of CD4+CD126lowFoxp3+ Treg immunotherapy in lupus nephritis is not clear. The purpose of this study was to explore the mechanism of CD4+CD126lowFoxp3+ Treg immunotherapy in mice with lupus nephritis. Method In vitro experiments CD4+CD126lowFoxp3+ Treg or CD4+CD126lowFoxp3+ Treg pretreated with PD-1 inhibitor were co-cultured with T or B lymphocytes of lupus mice under different in vitro culture condition. The expression levels of Akt and mTOR of Treg in each group were measured under immunoinflammatory conditions. To observe the effects and differences of Treg groups on the activation, proliferation and differentiation of T or B cells and other immunomodulatory effects. In vivo experiments CD4+CD126lowFoxp3+ Treg (2 × 106/mouse) and CD4+CD126lowFoxp3+ Treg (2 × 106/mouse) pretreated with PD-1 inhibitor and PBS were injected into NZM2328 lupus mice, respectively. After cell injection, urine protein was measured weekly. Autoantibody expression in lupus mice was measured every two weeks. The effects of Treg on the proliferation and differentiation of T/B cells in lupus mice were observed. The therapeutic effects of Treg on lupus mice were observed. Results Compared with CD4+CD126lowFoxp3+ Treg, the expression of Akt and mTOR increases in PD-1 inhibitors pretreatment cells. The activation, proliferation and differentiation functions of T or B lymphocytes of lupus mice were significantly weakened by immunosuppression of PD-1 inhibitors pretreated Treg in vitro, indicating that CD4+CD126lowFoxp3+ Treg may inhibit Akt-mTOR signaling pathway through PD-1 in in vitro. Compared with CD4+CD126lowFoxp3+ Treg, the activation, proliferation and differentiation functions of T or B lymphocytes of lupus mice were significantly weakened by immunosuppression of PD-1 inhibitors pretreated Treg in vivo. And its therapeutic effect on lupus mice was ineffective, indicating that CD4+CD126lowFoxp3+ Treg may inhibit Akt-MTOR signaling pathway through PD-1 in vivo. Conclusion CD4+CD126lowFoxp3+ Treg may inhibit the Akt-mTOR signaling pathway by expressing PD-1, and maintain stable immunomodulatory function in the inflammatory state, thus producing immunotherapeutic effect on lupus nephritis mice.

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