scholarly journals Evaluation of oxidative stress biomarkers in acute mercury intoxication

Folia Medica ◽  
2021 ◽  
Vol 63 (5) ◽  
pp. 704-709
Author(s):  
Tahir Dalkiran ◽  
Kursat Bora Carman ◽  
Velid Unsal ◽  
Ergul Belge Kurutas ◽  
Yasar Kandur ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Elemental Mercury ◽  
Mercury Exposure ◽  
Control Group ◽  
Oxidative Stress Biomarkers ◽  
Mercury Intoxication ◽  
Oxidative Stress Status ◽  
History Of ◽  
And Oxidative Stress ◽  
Gpx Activity

Objective:&nbsp;Very few studies have evaluated the association between mercury exposure and oxidative stress in humans, particularly in children. This is the first report where we aimed to determine the oxidative stress status of children who were accidently exposed to elemental mercury. Methods: In the present study, the study group was composed of 86 randomly selected children poisoned by mercury; the control group was composed of 78 children who had no history of mercury exposure. At admission, blood samples were collected. Blood superoxide dismutase activity, catalase enzyme activity, and glutathione peroxidase activity were measured by Fridovich, Beutler, and Lawrence Burk methods respectively, and the results were given as U/g Hb . Malondialdehyde level was measured by Ohkawa methods , and the results were given as mmol/ml. Results:&nbsp;Catalase levels were significantly lower in the patient group compared to the control group (1.28&plusmn;0.62 vs 3.90 &plusmn; 0.86 U/g Hb, p<0.01). In exposed children, SOD levels were significantly higher than the controls (5936 &plusmn; 810 vs 2226 &plusmn; 464 U/g Hb, p<0,05), while the GPx activity was significantly lower (13.01 &plusmn; 3.21&nbsp; vs 34.97 &plusmn; 7.32 U/g Hb, p<0.01). The MDA levels of the mercury group were significantly higher than the MDA levels of the control group (2.85&plusmn;0.84&nbsp; vs 2.05&plusmn;0.79 mmol/ml, p<0.05) . Conclusion: The results of the present study showed that acute mercury poisoning causes alteration of oxidative stress status in children exposed to elemental mercury.

scholarly journals Influence of supplemented coated-cysteamine on morphology, apoptosis and oxidative stress status of gastrointestinal tract

2019 ◽  
Vol 15 (1) ◽  
Author(s):  
Hongnan Liu ◽  
Miaomiao Bai ◽  
Bie Tan ◽  
Kang Xu ◽  
Rong Yu ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Caspase 3 ◽  
Control Diet ◽  
Control Group ◽  
Lesion Score ◽  
Positive Effects ◽  
Oxidative Stress Status ◽  
Mucosal Morphology ◽  
The Stability ◽  
And Oxidative Stress

Abstract Background Cysteamine was coated to cover its odor and maintain the stability. However, coated cysteamine (CC) has not been clearly evaluated for its effects on the gastrointestinal mucosa status. We hypothesize that the appropriate CC supplementation in diet impacts the stomach and intestinal mucosa variously through regulating the morphology, apoptosis, and oxidative stress status in model of pigs. Results The results showed that villus height increased (P < 0.05), and crypt depth decreased (P < 0.05) in the ileum when pigs were fed the diet with low cysteamine (LCS) compared with the control diet. The ileal lesion score in the LCS group was significantly (P < 0.01) lower than that in the control group, while the gastric lesion score in the CC group was significantly (P < 0.01) higher compared with that of the control group. It also showed that the activities of total superoxide dismutase (T-SOD) and diamine oxidase (DAO) were upregulated (P < 0.05) in the LCS group. In addition, Bax and caspase 3 immunore-activity increased (P < 0.01), and Bcl-2 immunoreactivity decreased (P < 0.01) in the gastric mucosa of pigs fed the diet with high cysteamine (HCS). The Bax and caspase 3 immunoreactivity decreased (P < 0.01), and Bcl-2 immunoreactivity increased (P < 0.01) in ileum mucosa of pigs fed the HCS diet. Conclusions Although moderate dietary coated cysteamine showed positive effects on GI mucosal morphology, apoptosis, and oxidative stress status, the excess coated cysteamine may cause apoptosis leading to GI damage in pigs.

Insulin-Leptin Axis, Cardiometabolic Risk and Oxidative Stress in Elderly with Metabolic Syndrome

2018 ◽  
Vol 126 (07) ◽  
pp. 445-452 ◽  
Author(s):  
Daniela Gradinaru ◽  
Husseina Khaddour ◽  
Denisa Margina ◽  
Anca Ungurianu ◽  
Claudia Borsa ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Metabolic Syndrome ◽  
Uric Acid ◽  
Resistance Index ◽  
Hdl Cholesterol ◽  
Elderly Subjects ◽  
Control Group ◽  
Oxidative Stress Biomarkers ◽  
The Metabolic Syndrome ◽  
And Oxidative Stress

AbstractInsulin and leptin have an overlapping anorexigenic action as well as opposite effects on glucose and lipid metabolism. The study focuses on the biochemical and clinical relevance of new indices of insulin-leptin axis utilized in the study of the relationships between leptinemia, insulin sensitivity and oxidative stress, in elderly subjects with metabolic syndrome. We conducted clinical studies on elderly people with metabolic syndrome versus control subjects by creating new insulin-adipogenic indices, namely Insulin-to-Leptin Ratio (ILR) and Insulin-Adipogenic Resistance index (IAR-index). Inflammation and oxidative stress biomarkers evaluated were the high-sensitivity C-reactive protein (hsCRP), the advanced oxidation protein products (AOPP), and the serum antioxidant capacity measured as ferric reducing antioxidant potential (FRAP). The metabolic syndrome group showed significantly (p<0.01) lower levels of ILR and not significant (p=0.09) higher values of IAR-index, as compared to the control group. In metabolic syndrome subjects, the IAR-index was significantly positively correlated with uric acid (r=0.313, p<0.05), FRAP (r=0.347, p<0.05) and AOPP (r=0.677, p<0.01), and negatively correlated with HDL-cholesterol (r=− 0.340, p<0.05) as well as with the ratio FRAP/uric acid (r=− 0.315, p<0.05). ILR and IAR-index reflected the biological state of adipose and pancreatic β-cells and seem to depict the adipo-insular axis status related to metabolic and oxidative stress better than individual markers. Therefore, ILR and IAR-index could represent integrated high-potential biomarkers for disease and patient stratification.

scholarly journals Proinflammatory and Oxidative Stress Markers in Patients with Periodontal Disease

2007 ◽  
Vol 2007 ◽  
pp. 1-5 ◽  
Author(s):  
Ivan Borges Jr. ◽  
Emília Addison Machado Moreira ◽  
Danilo Wilhem Filho ◽  
Tiago Bittencourt de Oliveira ◽  
Marcelo Barreto Spirelle da Silva ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Periodontal Disease ◽  
Gingival Tissue ◽  
Control Group ◽  
Myeloperoxidase Activity ◽  
Enzymatic Antioxidants ◽  
Oxidative Stress Markers ◽  
Oxidative Stress Biomarkers ◽  
Stress Markers ◽  
And Oxidative Stress

Objective. To evaluate the involvement of proinflammatory and oxidative stress markers in gingival tissue in individuals with chronic periodontitis.Subject and methods. Eighteen subjects were divided in two groups: experimental (age52.9±5.0) and control (age51.1±9.6). The activities of enzymatic antioxidants such as catalase, glutathione peroxidase (GPx), glutathione S-transferase (GST), glutathione reductase, nonenzymatic antioxidants: total glutathione and reduced glutathione, oxidized glutathione (GSSG), thiobarbituric acid reactive substances (TBARS), and myeloperoxidase activity (MPO) were evaluated in gingival tissues from interproximal sites. Statistical differences between groups were determined by independent Studentttest andP<.05.Results. Individuals with periodontal disease exhibited a significant increase in the activities of MPO, GPx, GST, and also in TBARS and GSSG levels in gingival tissue compared to the control group (P<.05).Conclusion. The results of the present work showed an important correlation between oxidative stress biomarkers and periodontal disease.

Serum lipoprotein profile and oxidative stress biomarkers in Wistar rats fed drinking water containing iron and copper

Biologia ◽  
2013 ◽  
Vol 68 (4) ◽  
Author(s):  
Alexey Tinkov ◽  
Alexandr Nikonorov
Keyword(s):  
Oxidative Stress ◽  
Drinking Water ◽  
Protein Oxidation ◽  
Wistar Rats ◽  
Free Radical Oxidation ◽  
Serum Lipoprotein ◽  
Control Group ◽  
Oxidative Stress Biomarkers ◽  
Stress Biomarkers ◽  
And Oxidative Stress

AbstractThe aim of the research was to estimate the effect of different doses and combinations of iron and copper consumption with drinking water on lipid profile and oxidative stress biomarkers in albino Wistar rats serum. Rats were given drinking water containing 3 mg L−1 and 6 mg L−1 iron; copper 4.88 and 9.76 mg L−1; a mixture of 3 mg L−1 iron and 4.88 mg L−1 copper. Control group obtained pure drinking water. Total cholesterol, lipoprotein spectrum and markers of lipid and protein oxidation were analyzed. It has been seen that administration of iron in concentration of 6 mg L−1 induces lipid peroxidation and protein oxidation, while copper given in the maximal doses leads only to protein oxidation. Free radical oxidation in rats obtaining combination of iron and copper with drinking water was more expressed than in case of administration of single metals in the same doses. Consumption of maximal doses of isolated metals leads to more expressed atherogenic changes, while combination of both metals in lower doses did not affect serum lipoprotein significantly. The data obtained show that chemical interaction of iron and copper in the organism has an additive effect on some vital parameters in comparison to isolated metal administration.

scholarly journals Salvia miltiorrhiza hydroalcoholic extract inhibits postoperative peritoneal adhesions in rats

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Abbas Raisi ◽  
Omid Dezfoulian ◽  
Farshid Davoodi ◽  
Shayan Taheri ◽  
Soroush Afshar Ghahremani
Keyword(s):  
Oxidative Stress ◽  
Salvia Miltiorrhiza ◽  
Control Group ◽  
Distilled Water ◽  
Oxidative Stress Biomarkers ◽  
Peritoneal Adhesions ◽  
Hydroalcoholic Extract ◽  
Treatment Groups ◽  
Stress Biomarkers ◽  
And Oxidative Stress

Abstract Background One of the most prevalent postoperative complications is believed to be intra-abdominal peritoneal adhesions, which is followed by several complications. Several adhesion prevention products have been examined, yet none of them were found to be completely effective. The current research is conducted to evaluate the beneficial effects of Salvia miltiorrhiza hydroalcoholic extract in inhibiting postoperative peritoneal adhesions in rats. Methods Forty rats were randomly classified into five equal groups (n = 8): 1) the normal group did not undergo surgical operations, 2) the control group in which the adhesion was induced, and which did not receive any treatment, 3) distilled water group that received distilled water, and 4,5) treatment groups treated with 1 and 5% of Salvia miltiorrhiza hydroalcoholic extract. The rats were euthanized 14 days following the surgery and the macroscopic score, the microscopic score of granulomatous inflammation and granulation tissue formation, IHC markers (vimentin, CD31, IL-1β, COX-2, and iNOS), and oxidative stress biomarkers (MDA, GPx, CAT, and TAC) were assessed in the experimental groups of the study. Results The difference between the control group and other groups for the adhesions macroscopic score, microscopic score, IHC markers, and oxidative stress biomarkers was significant (p < 0.05). Distilled water had no protective effect on the formation of peritoneal adhesions. Salvia miltiorrhiza treatment in two different doses significantly reduced macroscopic and microscopic scores, MDA concentration, Vimentin, IL-1β, COX-2, and iNOS compared to the control group (p < 0.05). The levels of GPx, CAT, and TAC in the treatment groups increased significantly compared with the control group (p < 0.05). Our findings revealed that a higher dose of Salvia miltiorrhiza was more effective in reducing peritoneal adhesions, proinflammatory and mesenchymal cell markers, and oxidative stress. Conclusions Salvia miltiorrhiza extract, owing to its strong antioxidant and anti-inflammatory properties, could effectively reduce peritoneal adhesions. Therefore, Salvia miltiorrhiza is recommended to be used as an effective anti-peritoneal post-operative adhesive agent.

scholarly journals Occupational exposure in lead and zinc mines induces oxidative stress in miners lymphocytes: Role of mitochondrial/lysosomal damage

2020 ◽  
Vol 43 (1) ◽  
pp. 154-163
Author(s):  
Enayatollah Seydi ◽  
Mahshid Soltani ◽  
Maral Ramazani ◽  
Mohammad Hadi Zarei ◽  
Jalal Pourahmad
Keyword(s):  
Oxidative Stress ◽  
Control Group ◽  
Lead And Zinc ◽  
Toxic Agents ◽  
Oxidative Stress Status ◽  
Lead Zinc ◽  
Zinc Mine ◽  
And Oxidative Stress ◽  
Mine Workers

AbstractThe purpose of this research was to determine mitochondrial and lysosomal damage and oxidative stress status in blood lymphocytes of lead-zinc miners. This research was performed in 10 mine workers who have been in contact with lead and zinc in comparison to a control group containing 10 healthy volunteers. Lymphocytes were isolated from peripheral blood using the Ficoll standard method and then mitochondrial and lysosomal damage and oxidative stress were evaluated. The level of reactive oxygen species (ROS), collapse in the mitochondrial membrane potential (MMP) collapse, and glutathione disulfide (GSSG) content, and lysosomal damage in miners were higher than the control group. Also, viability and glutathione (GSH) content were decreased. The lymphocytes of workers of a lead-zinc mine are more susceptible to oxidative stress, mitochondrial and lysosomal damage. The proper use of safety equipment can reduce the risk of toxic agents and their subsequent hazards for mine workers.

scholarly journals To Study The Serum Vitamin –D And Oxidative Stress Status In Rheumatoid Arthritis

2017 ◽  
Vol 1 (2) ◽  
Author(s):  
T Uma
Keyword(s):  
Rheumatoid Arthritis ◽  
Oxidative Stress ◽  
Vitamin D ◽  
Vitamin C ◽  
Joint Destruction ◽  
Serum Vitamin ◽  
Control Group ◽  
Healthy Controls ◽  
Oxidative Stress Status ◽  
And Oxidative Stress

Aim and objectives: Rheumatoid arthritis (RA) is a chronic autoimmune systemic disease characterized predominantly by synovial inflammation, leading to joint destruction. The study was undertaken to analyze the vitamin –D and oxidative stress status in Rheumatoid arthritis   Materials and methods: The Study includes Patients diagnosed as RA by using RA factor within the age group of30 to 60 years and comprised of 20 age matched control group. To assess oxidative stress, serum Malonyldialdehyde (MDA) was by thiobarbituric acid method and antioxidant level was assessed by estimation of serum vitamin C by dinitrophenyl hydrazine method. And vitamin D total is estimated by chemiluminescent immunoassay. Results: There is a significant decreased level of vitamin -D in the RA patients (37.58±5.7ng/ml) as compared to healthy controls (58.5±5.6ngl/ml). There is a  a significant increased levels of  MDA in the RA patients (7.6±1.09) as compared to healthy controls (8.4±2.8). There is a significant decreased level of vitamin -c in the RA patients (0.6±0.3mg/dl) as compared to healthy controls (1.0±0.31mg/dl).   Conclusion: Low levels of Vitamin D deficiency in RA patients is associated  with inflammation which leads to Increased oxidative stress status , which may lead to connective tissue degradation leading to joint and periarticular deformities in rheumatoid arthritis.

Oxidative Stress Status in Patients with Choledocholithiasis: Before and After Endoscopic Sphincterotomy and Biliary Clearance

2018 ◽  
Vol 69 (8) ◽  
pp. 2172-2176
Author(s):  
Catalin Victor Sfarti ◽  
Alin Ciobica ◽  
Carol Stanciu ◽  
Gheorghe G. Balan ◽  
Irina Garleanu ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Endoscopic Sphincterotomy ◽  
Specific Activity ◽  
Oxidative Stress Marker ◽  
Control Group ◽  
Oxidative Stress Parameter ◽  
Extrahepatic Cholestasis ◽  
Biliary Clearance ◽  
Oxidative Stress Status ◽  
Before And After

Choledocholithiasis may cause biliary obstruction which leads to hepatocellular injury. Oxidative stress has been proposed as a possible mechanism involved in this disorder. This study evaluates the oxidative stress burden in patients with choledocholithiasis and secondary cholestasis, before and after endoscopic sphincterotomy. Experimental part: Patients diagnosed with choledocholithiasis and secondary extrahepatic cholestasis were included in the study between January 1st 2016 and October 31st 2016. In all patients oxidative stress markers were collected within 2 hours before and 48 hours after therapeutic ERCP. Selected markers were superoxide dismutase (SOD), glutathione peroxidase (GPX) and malondialdehyde (MDA). The results were compared to those from a group of 40 healthy subjects. Significantly lower concentrations of SOD (p = 0.03) and GPX (p [ 0.0001) activities, associated with an increased level of MDA level (p [ 0.0001) were shown in patients before biliary clearance compared with the healthy control group. After ERCP the only oxidative stress parameter which showed improvement was the SOD specific activity (p = 0.037). This study shows that extrahepatic cholestasis secondary to choledocholithiasis is associated with increased oxidative stress status. After biliary clearance one oxidative stress marker was significantly improved (SOD), suggesting a possible antioxidant effect of such procedure.

Evaluation of Salivary Antioxidants and Oxidative Stress Markers in Type 2 Diabetes Mellitus: A Retrospective Cohort Study

2020 ◽  
Vol 20 (4) ◽  
pp. 584-590 ◽  
Author(s):  
Shima Fathi ◽  
Shiva Borzouei ◽  
Mohammad Taghi Goodarzi ◽  
Jalal Poorolajal ◽  
Fatemeh Ahmadi-Motamayel
Keyword(s):  
Oxidative Stress ◽  
Diabetes Mellitus ◽  
Control Group ◽  
Oxidative Stress Markers ◽  
Stress Markers ◽  
Type 2 Dm ◽  
Patients With Diabetes ◽  
The Difference ◽  
And Oxidative Stress

Background: Diabetes Mellitus (DM) is a progressive metabolic disorder. Objective: The aim of this study was to investigate the relationship between antioxidant and oxidative stress markers in the saliva of patients with type 2 DM and a healthy control group. Methods: In this study, 20 patients with diabetes and 20 healthy individuals were evaluated. Salivary antioxidants markers consisted of total antioxidant capacity (TAC), uric acid (UA), peroxidase and catalase. Oxidative stress markers included total oxidant status (TOS), malondealdehyde (MDA) and total thiol (SH). Sialochemical analysis was performed with spectrophotometric assay. All the statistical analyses were conducted using STATA software. Results: TAC decreased significantly in patients with diabetes. Although salivary UA and peroxidase were lower in patients with diabetes compared to the control group, the difference was not significant. Salivary catalase in patients with diabetes was significantly lower than that in the control group. MDA and TOS exhibited significantly higher levels in type 2 DM. SH levels were slightly higher in DM. Conclusions: According to the results of the present study, there were some changes in the salivary levels of some antioxidants and oxidative stress markers in patients with type 2 DM and could be measured as an indicator of serum changes..

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