scholarly journals Metabarcoding of soil nematodes: the importance of taxonomic coverage and availability of reference sequences in choosing suitable marker(s)

2019 ◽  
Vol 3 ◽  
Author(s):  
Mohammed Ahmed ◽  
Matthew Alan Back ◽  
Thomas Prior ◽  
Gerrit Karssen ◽  
Rebecca Lawson ◽  
...  
Keyword(s):  
Poor Performance ◽  
Genomic Region ◽  
The Other ◽  
Reference Sequence ◽  
Soil Nematodes ◽  
Good Resolution ◽  
Sequence Collection ◽  
Reference Sequences ◽  
Suitable Marker ◽  
Specific Genomic Region

For many organisms, there is agreement on the specific genomic region used for developing barcode markers. With nematodes, however, it has been found that the COI region designated for most animals lacks the taxonomic coverage (ability to amplify a diverse group of taxa) required of a metabarcoding marker. For that reason, studies on metabarcoding of nematodes thus far have utilized primarily regions within the highly conserved 18S ribosomal DNA. Two popular markers within this region are the ones flanked by the primer pairs NF1-18Sr2b and SSUF04-SSUR22. The NF1-18Sr2b primer pair, especially, has been critiqued as not being specific enough for nematodes leading to suggestions for other candidate markers while the SSUF04-SSUR22 region has hardly been tested on soil nematodes. The current study aimed to evaluate these two markers against other alternative ones within the 28S rDNA and the COI region for their suitability for nematode metabarcoding. The results showed that the NF1-18Sr2b marker could offer wide coverage and good resolution for characterizing soil nematodes. Sufficient availability of reference sequences for this region was found to be a significant factor that resulted in this marker outperforming the other markers, particularly the 18S-based SSUFO4-SSUR22 marker. None of the other tested regions compared with this marker in terms of the proportion of the taxa recovered. The COI-based marker had the lowest number of taxa recovered, and this was due to the poor performance of its primers and the insufficient number of reference sequences in public databases. In summary, this study highlights how dependent the success of metabarcoding is on the availability of a good reference sequence collection for the marker of choice as well as its taxonomic coverage.

Orosensory Perception, Speech Production, and Deafness

1973 ◽  
Vol 16 (2) ◽  
pp. 257-266 ◽  
Author(s):  
Milo E. Bishop ◽  
Robert L. Ringel ◽  
Arthur S. House
Keyword(s):  
High School ◽  
Speech Production ◽  
Poor Performance ◽  
Normal Hearing ◽  
The Other ◽  
Functional Disorders ◽  
Form Discrimination ◽  
School Subjects ◽  
Oral Form ◽  
Similarities And Differences

The oral form-discrimination abilities of 18 orally educated and oriented deaf high school subjects were determined and compared to those of manually educated and oriented deaf subjects and normal-hearing subjects. The similarities and differences among the responses of the three groups were discussed and then compared to responses elicited from subjects with functional disorders of articulation. In general, the discrimination scores separated the manual deaf from the other two groups, particularly when differences in form shapes were involved in the test. The implications of the results for theories relating orosensory-discrimination abilities are discussed. It is postulated that, while a failure in oroperceptual functioning may lead to disorders of articulation, a failure to use the oral mechanism for speech activities, even in persons with normal orosensory capabilities, may result in poor performance on oroperceptual tasks.

scholarly journals Study on Temperament of Scholastically Backward Children

2017 ◽  
Vol 4 (2) ◽  
Author(s):  
Dr. Jitendra Mugali ◽  
Dr. Nitin Pattanashetty ◽  
Dr. S S Chate ◽  
Dr. N M Patil ◽  
Dr. Sandeep Patil ◽  
...  
Keyword(s):  
Statistical Analysis ◽  
Early Detection ◽  
Poor Performance ◽  
Final Diagnosis ◽  
School Refusal ◽  
The Other ◽  
Scholastic Performance ◽  
Major Factors ◽  
Drop Outs ◽  
Psychiatric Problems

Objectives: 1.To studies the categorical prevalence of temperament of scholastically backward children. 2. To study the associated Psychiatric problems with type of temperament in scholastically backward children. Material and Methods: Total of 1480 children studying in 3 CBSE schools, aged between 6 and 12 years were screened. 312 children were found to scholastically backward. After simple randomization and further screening procedure 115 children were included in the study. Each child was assessed by interviewing with MINI-KID Questionnaire, temperament assessment scale, I Q assessment by using Reven’s coloured progressive matrices, CBCL and final diagnosis done by ICD DCR criteria.  Statistical analysis was done using Epi Info 7 software. Results: The prevalence of scholastically backwardness was found to be 21.08%. The distribution of scholastically backward children belongs to falling types of temperament found to be easy temperament-41.74 %, difficult temperament 13.91 % and slow to warm temperament 44.35% respectively. Majority of scholastically backward children belongs to slow to warm type of temperament. All the difficult temperament children were associated with one or the other psychiatric problems. 18.75% of children with easy temperament and 74.51% of children with slow to warm are associated with psychiatric problems. Conclusion: Temperament of a child is one of the major factors for scholastic performance. Some children do poor performance in academics, school refusal and school drop outs. Prevalence of difficult temperament in children had associated with 100% psychiatric disorder. Difficult temperament children handling in home and in school is very difficult, so early detection of associated psychiatric problems and early intervention will surely help to handle the further consequences.

scholarly journals Sequence Analysis of Bacterial Artificial Chromosome Clones from the Apospory-Specific Genomic Region of Pennisetum and Cenchrus

2008 ◽  
Vol 147 (3) ◽  
pp. 1396-1411 ◽  
Author(s):  
Joann A. Conner ◽  
Shailendra Goel ◽  
Gunawati Gunawan ◽  
Marie-Michele Cordonnier-Pratt ◽  
Virgil Ed Johnson ◽  
...  
Keyword(s):  
Sequence Analysis ◽  
Bacterial Artificial Chromosome ◽  
Artificial Chromosome ◽  
Genomic Region ◽  
Specific Genomic Region

Credibility in Sustainable Aquaculture Eco-Labeling

2019 ◽  
pp. 81-116
Author(s):  
Hamish van der Ven
Keyword(s):  
Best Practice ◽  
Geographic Location ◽  
Poor Performance ◽  
The Other ◽  
Process Tracing ◽  
Direct Result ◽  
Sustainable Aquaculture ◽  
Origin And Evolution ◽  
Domestic Markets ◽  
Comparative Case Studies

This chapter tests the aiming big hypothesis by tracing the origin and evolution of four eco-labeling organizations in sustainable aquaculture: the Aquaculture Stewardship Council (ASC), Best Aquaculture Practices (BAP), Friend of the Sea (FOS), and Naturland. The chapter argues that stronger adherence to best practices in the ASC and BAP programs is a direct result of their decision to target large multinational retailers for certification. Conversely, the comparatively poor performance of the other two eco-labeling organizations can be traced to their decisions to target smaller firms in domestic markets. The chapter uses comparative case studies and within-case process tracing to reveal how decisions about who to target for certification impacted best practice adherence through the causal mechanisms outlined in chapter 3. The four cases analyzed in this chapter cast further doubt on alternate theories of credibility, such as the relevance of ownership or geographic location to credibility.

scholarly journals Comparative analyses of stevioside between fresh leaves and in-vitro derived callus tissue from Stevia rebaudiana Bert. using HPLC

2015 ◽  
Vol 49 (4) ◽  
pp. 199-204 ◽  
Author(s):  
S Mahmud ◽  
S Akter ◽  
IA Jahan ◽  
S Khan ◽  
A Khaleque ◽  
...  
Keyword(s):  
Retention Time ◽  
Callus Tissue ◽  
Callus Formation ◽  
Leaf Explants ◽  
Stevia Rebaudiana ◽  
Poor Performance ◽  
The Other ◽  
Ms Medium ◽  
Green Callus

A protocol was developed to produce large amount of callus in short a period of time from leaf explants of Stevia rebaudiana Bert. The highest amount of white callus was obtained on MS medium supplemented with 2.5 mg/l 2, 4-D and 0.5 mg/l BAP after 3 weeks of inoculating leaf segments. On the other hand, 0.5 mg/l BAP and 1.0 mg/l Kn exhibits poor performance towards callus formation while after using 1.0 mg/l Kn alone did not develop any callus. In this experiment, highest amount of green callus was obtained when MS medium supplemented with 2.5 mg/l NAA and 10% coconut water was used. An improved analytical method HPLC was applied to analyze stevioside extracted from the leaf and callus of Stevia rebaudiana. The stevioside in each sample were analyzed by comparing their retention times with those of the standards. The retention time (RT) of stevioside for leaves were found 14.96 and for callus 13.81 mins. The percentage of stevioside content from leaves and callus was 12.19% and 12.62% respectively DOI: http://dx.doi.org/10.3329/bjsir.v49i4.22621 Bangladesh J. Sci. Ind. Res. 49(4), 199-204, 2014

Relocation does not have a significant effect on the growth rate of Bos indicus cross steers

2008 ◽  
Vol 48 (5) ◽  
pp. 608 ◽  
Author(s):  
R. G. Holroyd ◽  
V. J. Doogan ◽  
M. R. Jeffery ◽  
J. A. Lindsay ◽  
B. K. Venus ◽  
...  
Keyword(s):  
Growth Rate ◽  
Phase 1 ◽  
Bos Indicus ◽  
Poor Performance ◽  
The Other ◽  
Control Group ◽  
Phase 2 ◽  
Treatment Groups ◽  
Normal Ranges ◽  
Native Pastures

This experiment tested the hypothesis that relocating cattle is detrimental to their growth. The study examined the effect of having relocated cattle mixed with, or segregated from, the local acclimatised cattle at the destination property. Bos indicus cross steers (120) were allocated to three groups and were relocated, in two separate cohorts, 980 km from northern Queensland to improved pastures in central Queensland. At the start of Phase 1, the control group (C) was moved 3 months before the other two groups. The remaining two groups grazed native pastures; one group was supplemented (SR) to increase growth rate similar to that expected from improved pasture in central Queensland and the other was not supplemented (R). At the end of Phase 1, C was significantly (P < 0.05) heavier than SR, which was significantly (P < 0.05) heavier than R. At the start of Phase 2, the SR and R groups were relocated and after transportation the R and SR groups lost 12 kg or 4.4% of liveweight and 18 kg or 5.7% of liveweight, respectively; this weight loss was recovered after 5 days. All steers were reallocated to segregated (SEG) or mixed (MIX) treatment groups forming six treatments (SEG.C, SEG.R and SEG.SR and MIX.C, MIX.R and MIX.SR). There were no significant differences in liveweights within the SEG treatments by 57 days or within the MIX treatments by 106 days after relocation. There were few if any significant differences in the plasma constituents and differential leucocyte counts of the steers and most results were within physiologically normal ranges. We conclude on the basis of these results and of other experiments that the anecdotal poor performance of cattle after relocation appears to be unfounded.

scholarly journals Quantifying the Errors Involved in Transferring Suspended Sediment Rating Equations Across Ablation Seasons

1989 ◽  
Vol 13 ◽  
pp. 64-68 ◽  
Author(s):  
C.R. Fenn
Keyword(s):  
Transfer Function ◽  
Regression Model ◽  
Suspended Sediment ◽  
Simple Structure ◽  
Transfer Functions ◽  
Poor Performance ◽  
Generalized Least Squares ◽  
The Other ◽  
The Poor ◽  
Suspended Loads

Bias-corrected suspended sediment rating equations are developed for the data from six separate ablation seasons in the Glacier de Tsidjiore Nouve basin. These equations differ significantly from one another. Suspended loads as predicted by equations used beyond their year of origin fall in the range of 34-278% of the true load. Mean absolute-percentage errors (MAPE) based on forecasts computed from each year’s model applied to the other 5 years of data range from 35 to 81%, with a mean of 52%. The equivalent MAPE for a lumped multi-year model is 38%, for an autocorrelation-correcting generalized least-squares (GLS) regression model, is 15%, and for a transfer function (TF) developed from a single 25 d period, only 5%. Simple structure-transfer functions appear to be more robust to temporal transfer than the usual type of rating equation, and offer the possibility of greatly improving upon the poor performance of OLS rating equations in providing estimates of suspended loads for periods beyond frames of origin.

scholarly journals B-74 Using Rates of Low Scores to Assess Agreement between ANAM4 TBI-MIL and a Traditional Battery of Neuropsychological Tests

2019 ◽  
Vol 34 (6) ◽  
pp. 1022-1022
Author(s):  
B Ivins ◽  
J Arrieux ◽  
W Cole ◽  
K Schwab
Keyword(s):  
Traumatic Brain Injury ◽  
Neuropsychological Tests ◽  
Poor Performance ◽  
The Other ◽  
Neurological Assessment ◽  
Us Military ◽  
The Us ◽  
Valid Data ◽  
Overall Performance ◽  
Assessment Metrics

Abstract Objective The Automated Neurological Assessment Metrics (Version 4) Traumatic Brain Injury Military (ANAM4 TBI-MIL) battery is widely used by the US military to assess cognitive function. We compare intra-individual agreement between ANAM4 TBI-MIL and a battery of traditional neuropsychological tests using rates of low scores. Methods Complete and valid data from 246 healthy soldiers and 177 soldiers ≤7 days from sustaining mild TBI (mTBI) were used in this analysis. All soldiers were consecutively administered ANAM4 TBI-MIL and a traditional test battery consisting of: TOPF, WAIS-IV, CVLT-II, RCFT, DKEFS, and CPT-II. We performed base rate analyses of both batteries to determine the proportions of soldiers who had various numbers of scores that were 1.0+, 1.5+, and 2.0+ standard deviations below the normative mean. We used those rates to place Soldiers into a “low score hierarchy” ranging from the least poor (i.e. ~≥10th %ile) to the worst overall performance (i.e. ~≤10th %ile). We then compared agreement between the batteries at each of those levels. Results More soldiers with mTBI had low scores than healthy soldiers on both batteries. Of the soldiers who performed at the worst level on one battery, 88.1% from ANAM and 100% from traditional had some level of poor performance on the other battery. However, of the soldiers who performed at the worst level on either battery, only 58.3% from ANAM and 50.0% from traditional also performed at the worst level on the other battery. Conclusion These batteries similarly identify poor performance to a degree, though with some potentially meaningful differences still present.

QuanTest2: benchmarking multiple sequence alignments using secondary structure prediction

2019 ◽  
Author(s):  
Fabian Sievers ◽  
Desmond G Higgins
Keyword(s):  
Secondary Structure ◽  
Structure Prediction ◽  
Secondary Structure Prediction ◽  
Reference Sequence ◽  
Supplementary Information ◽  
Sequence Alignments ◽  
Multiple Sequence ◽  
Multiple Sequence Alignments ◽  
Reference Sequences ◽  
Selection Of

Abstract Motivation Secondary structure prediction accuracy (SSPA) in the QuanTest benchmark can be used to measure accuracy of a multiple sequence alignment. SSPA correlates well with the sum-of-pairs score, if the results are averaged over many alignments but not on an alignment-by-alignment basis. This is due to a sub-optimal selection of reference and non-reference sequences in QuanTest. Results We develop an improved strategy for selecting reference and non-reference sequences for a new benchmark, QuanTest2. In QuanTest2, SSPA and SP correlate better on an alignment-by-alignment basis than in QuanTest. Guide-trees for QuanTest2 are more balanced with respect to reference sequences than in QuanTest. QuanTest2 scores correlate well with other well-established benchmarks. Availability and implementation QuanTest2 is available at http://bioinf.ucd.ie/quantest2.tar, comprises of reference and non-reference sequence sets and a scoring script. Supplementary information Supplementary data are available at Bioinformatics online

Congruence of Causal Attributions for School Performance Given by Children and Mothers

1989 ◽  
Vol 64 (2) ◽  
pp. 359-363 ◽  
Author(s):  
Hirotsugu Yamauchi
Keyword(s):  
School Performance ◽  
Task Difficulty ◽  
Causal Attributions ◽  
Causal Attribution ◽  
Poor Performance ◽  
The Other ◽  
Seventh Grade ◽  
Response Options ◽  
Good School ◽  
Poor School

The purpose of this study was to compare the attributions of children with mothers' attributions and children's predictions of their mothers' attributions for children's school performances. Subjects were 76 boys and 94 girls in seventh grade and their mothers. The questionnaire was composed of 8 items and each item had 4 response options to evaluate factors of ability, effort, task difficulty, or luck. Four items were related to good school performance and the other four to poor performance. Children and mothers attributed both good and poor school performances to effort. Several tendencies of children's causal attribution were congruent with the tendencies in children's predictions of their mothers' attributions but were not related to mothers' actual attributions.

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