Investigation of the bischofite gel reparative effect on the linear wound model

Introduction. The currently available information on the bischofite mineral pharmacodynamics makes it possible to assume that bischofite-based gels have wound-healing properties. Materials and methods. Experiments were performed on 36 male rats. Using a blade, a linear wound of 50 mm long was modeled. Animals were divided into 4 groups: 1) Control; 2) Bishofit (500 mg of gel with bischofite on the wound area for 7 days); 3) Actovegine (500 mg of Actovegine gel on the wound area for 7 days); 4) Contractubex (500 mg of contractubex gel on the wound area for 7 days). The wound healing effect of the drugs was evaluated through studying the physicomechanical properties, assessing the morphological picture, determining the concentration of hydroxyproline (HP) and calculating the ratio of types I and III collagen. Results. The strongest healing effect on the wound defect, significantly greater than that in the control (p<0.01), was obtained in the group that received bischofite gel (13.70±0.76 N). Actovegine also demonstrated a positive reparative effect (12.60±0.63 N, p<0.05). Significantly lower (p<0.01) healing effect than in the control group was obtained in the group of animals that had received contractubex (9.65±0.59 N). In the calorimetric analysis, it was found that the highest concentration of HP was in the tissues of wound defects in animals treated with contractubex; however, there was no statistically significant difference with the control group. Significantly lower in comparison with the control (p<0.05) concentration of HP, was found in tissues of simulated wounds in animals treated with bischofite gel (79.7% of the control). When assessing the ratio of types I and III collagen in the tissues of the wound defect, when stained with picrosirius red, it was found that by the number of mature collagen fibers, the studied groups can be arranged in the following descending order: Bishofite> Actovegine> Control> Contractubex. A similar trend is demonstrated by the morphological picture of tissues in the area of the wound defect. Conclusion. The study showed that the best results were obtained with external use of bischofite gel. Actovegine has a less significant, but quite pronounced reparative, effect on this model. The least satisfactory results were obtained when applying contractubex.

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Wound Healing Potential of Crocin and Safranal, Main Saffron (Crocus sativus L.), the Active Constituents in Excision Wound Model in Rats

Galen Medical Journal ◽

10.31661/gmj.v10i0.1900 ◽

2021 ◽

Vol 10 ◽

Author(s):

Nafise Deldar ◽

Malihezaman Monsefi ◽

Mohsen Salmanpour ◽

Mohadeseh Ostovar ◽

Mojtaba Heydari

Keyword(s):

Wound Healing ◽

Crocus Sativus ◽

Control Group ◽

Topical Formulation ◽

Adult Rats ◽

Wound Area ◽

Active Constituents ◽

Healing Effect ◽

Wound Model ◽

Wound Healing Effect

Background: Saffron is traditionally suggested for wound healing in Persian medicine. It is investigated for wound healing effect in multiple studies with promising results. It is not examined that which ingredient of saffron contributes more to this effect. This study was aimed to evaluate and compare the wound healing potential of saffron and its active constituents, crocin, and safranal, in rats. Materials and Methods: Forty female adult rats with induced excision wounds were randomly divided into four groups to receive topical formulation of saffron, crocin, safranal, and placebo for seven days. The Wound area and histopathologic stage of wound healing were evaluated as outcome measures. Results: The wound area was significantly lower in treatment groups (saffron, crocin, and safranal) compared to the control group on day 7 of the intervention. Compared to the control group, the wound in all treated groups showed a decreased inflammatory response and more progression to the proliferation phase. The saffron group showed more advanced healing phase with complete epithelialization of the wound on day 7 of study when partial and no epithelialization was observed in safranal and crocin groups. Conclusion: The study showed the wound healing properties of both safranal and crocin with the slight superiority of safranal. However, saffron seems to be more potent in wound healing effect compared to safranal and crocin as its main active constituents. [GMJ.2021;10:e1900]

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673 Effect of Subcutaneous Topical Ozone Therapy on Second Degree Burn Wounds in Rats: An Experimental Study

Journal of Burn Care & Research ◽

10.1093/jbcr/irab032.319 ◽

2021 ◽

Vol 42 (Supplement_1) ◽

pp. S193-S193

Author(s):

Emre Karakaya ◽

Aydincan Akdur ◽

H Ebru ◽

Ayvazoglu Soy ◽

Alev Ok Atilgan ◽

...

Keyword(s):

Wound Healing ◽

Healing Process ◽

Silver Sulfadiazine ◽

Male Rats ◽

Control Group ◽

Ozone Therapy ◽

Burn Wounds ◽

Degree Burn ◽

Significant Difference ◽

Second Degree

Abstract Introduction Burn is one of the most severe traumas that causes coagulative destruction of the skin. The use of various products that accelerate wound healing in patients with burn may affect the patient’s survival and reduce the complications that may be seen. In the present study we aimed effects of subcutaneous ozone injection on second degree burn wound. Methods A total of 72 Sprague-Dawley male rats included in the study were divided randomly into three groups (control group (CG), silver sulfadiazine group (SG), ozone group (OG)) and each group was divided randomly two subgroups (as sacrificed on d7 and on d14).A deep second degree scald burns were created on the lower back. In CG subcutaneous 0.9% serum saline was injected daily into the burn area. In SG, burns were dressed with silver sulfadiazine daily and in OG subcutaneous ozone was injected daily into the burn area. Tissue hydroxyproline level measurement and histopathological evaluation were done. Results When the groups were compared in terms of weight change, no significant difference was found on the 7th and 14th days. In the evaluation made in terms of tissue hydroxyproline, tissue hydroxyproline level in OG was found to be significantly higher on both the 7th and 14th days (p < 0.001). In histopathological evaluations, it was determined that wound healing in OG was significantly higher than in the other groups. Conclusions According to the results, subcutaneous ozone therapy is more effective than silver sulphadiazine in the healing process of second-degree burn wounds and it can be safely used in the treatment of burn wounds.

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Pharmacological Investigation of the Wound Healing Activity of Cestrum nocturnum (L.) Ointment in Wistar Albino Rats

Journal of Pharmaceutics ◽

10.1155/2016/9249040 ◽

2016 ◽

Vol 2016 ◽

pp. 1-8 ◽

Cited By ~ 6

Author(s):

Hemant Kumar Nagar ◽

Amit Kumar Srivastava ◽

Rajnish Srivastava ◽

Madan Lal Kurmi ◽

Harinarayan Singh Chandel ◽

...

Keyword(s):

Wound Healing ◽

Ethanolic Extract ◽

Negative Control Group ◽

Negative Control ◽

Control Group ◽

Albino Rats ◽

Healing Effect ◽

Wound Model ◽

Wound Healing Effect ◽

Wistar Albino Rats

Objectives. The present study was aimed at investigating the wound healing effect of ethanolic extract of Cestrum nocturnum (L.) leaves (EECN) using excision and incision wound model. Methods. Wistar albino rats were divided into five groups each consisting of six animals; group I (left untreated) considered as control, group II (ointment base treated) considered as negative control, group III treated with 5% (w/w) povidone iodine ointment (Intadine USP), which served as standard, group IV treated with EECN 2% (w/w) ointment, and group V treated with EECN 5% (w/w) ointment were considered as test groups. All the treatments were given once daily. The wound healing effect was assessed by percentage wound contraction, epithelialization period, and histoarchitecture studies in excision wound model while breaking strength and hydroxyproline content in the incision wound model. Result. Different concentration of EECN (2% and 5% w/w) ointment promoted the wound healing activity significantly in both the models studied. The high rate of wound contraction (P<0.001), decrease in the period for epithelialization (P<0.01), high skin breaking strength (P<0.001), and elevated hydroxyproline content were observed in animal treated with EECN ointments when compared to the control and negative control group of animals. Histopathological studies of the EECN ointments treated groups also revealed the effectiveness in improved wound healing. Conclusions. Ethanolic extract of Cestrum nocturnum (EECN) leaves possesses a concentration dependent wound healing effect.

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Wound-healing effect of Platycodon grandiflorus (Jacq.) extract in rats

Tropical Journal of Pharmaceutical Research ◽

10.4314/tjpr.v18i9.18 ◽

2021 ◽

Vol 18 (9) ◽

pp. 1909-1912

Author(s):

Liang Wang ◽

Li Yang ◽

Gang Yuan ◽

Long-Jiao Hu

Keyword(s):

Wound Healing ◽

Treated Group ◽

Control Group ◽

Type Iii Collagen ◽

Matrix Metalloprotease ◽

Wound Area ◽

Burn Wounds ◽

Type Iii ◽

Healing Effect ◽

Wound Tissue

Purpose: To evaluate the healing effect of Platycodon grandiflorus (Jacq.). extract (PGE) on experimental burn wounds in rats. Methods: Rats were randomly divided into four groups of eight rats each: control group, silver sulfadiazine (SSD)-treated group, moist exposed burn ointment (MEBO)-treated group and PGE-treated group. PGE, SSD and MEBO were applied topically twice daily for 7 days. SSD and MEBO were used as reference control. External observation of wound area contraction and histological analysis of wound tissues was performed respectively. The effect of PGE on matrix metalloproteases (MMPs), vascular endothelial growth factor (VEGF) and Type-III Collagen proteins of wound tissue in rats were analysed by Western blot. Results: After 10 days of topical treatment with PGE, PGE-treated group showed faster reduction in wound area when compared with control groups (p < 0.01). Matrix metalloprotease-2 (MMP-2), matrix metalloprotease-9 (MMP-9), VEGF and type-III collagen expressions in the wound tissue increased significantly (p < 0.05) when compared with the control burn wounds. Histological results showed an overall early recovery and regeneration in PGE-treated group when compared with control group. Conclusion: PGE possesses a significant wound-healing activity in full-thickness burn wounds in rats. Therefore, it can potentially be developed for the management of burns.

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EFEK SALEP EKSTRAK PINANG TERHADAP LEVEL FIBROBLAST DAN KOLAGEN PADA PROSES PENYEMBUHAN LUKA

JAMBI MEDICAL JOURNAL Jurnal Kedokteran dan Kesehatan ◽

10.22437/jmj.v7i1.7121 ◽

2019 ◽

Vol 7 (1) ◽

pp. 19-25

Author(s):

Ave Olivia Rahman ◽

Humaryanto Humaryanto

Keyword(s):

Wound Healing ◽

Male Rats ◽

Control Group ◽

Betel Nut ◽

Full Thickness ◽

Wound Area ◽

Treatment Groups ◽

Areca Catechu ◽

Collagen Level ◽

Full Thickness Wound

ABSTRACT Background: Some plants, such as betel nuts (Areca catechu), is used as traditional antiseptic. Betel nut mash is applied on ulcus wound. Fibroblast and collagen are important factor in wound healing. Research objective : this study is to determine the effect of betel nuts extract on fibroblast and collagen level in full thickness wound healing processs. Methodology: this study used male rats Sprague dawney that randomly divided into 3 groups. Groups I received no treatment, groups II and III received areca catechu extracts with concentration 15% and 30% respectively. There are 12 rats in each groups. The treatment is given every day without wound’s debridement. Incision were made full thickness with diameter size 1,5 cm, on right back skin was made by lidocain anesthesia subcutaneously. Wound area were measured every days. Termination of rats were done in day 7 and day 16 to histopathology assessment with Haematoxylin-Eosin stain for fibroblast and collagen level by semiqualitative score. Results: level of collagen were higher in group that received extract but level of fibroblast were lower than control group in histopathology of day 7th. Conclusion: Extract of betel nut increased level of collagen. Keyword : areca catechu, betel nuts, wound, fibroblast, collagen, histopathology ABSTRAK Latar belakang : Beberapa tanaman digunakan sebagai antiseptik luka, salah satunya biji pinang untuk penyembuhan luka ulkus. Fibroblast dan kolagen merupakan salah satu faktor penting dalam penyembuhan luka. Tujuan : Penelitian ini bertujuan mengetahui efek ekstrak biji pinang terhadap level fibroblast dan kolagen pada proses penyembuhan luka full thickness Metode : Penelitian ini menggunakan tikus galur Sprague dawney jantan berusia 2-3 bulan dan telah mendapatkan persetujuan etik. Setelah aklimatisasi, tikus dibagi secara acak masing-masing 12 ekor dalam 3 kelompok, yaitu kelompok I tanpa perlakuan, kelompok II diberikan salep ekstrak biji pinang 15%, kelompok III diberikan salep ekstrak biji pinang 30%. Luka full thickness dibuat dengan diameter 1,5 cm di daerah punggung belakang bagian kanan dengan anestesi lidokain subkutan. Perlakuan dilakukan setiap hari tanpa debridemen luka. Luas luka diukur setiap hari. Terminasi dilakukan pada hari ke 7 dan 16 untuk pemeriksaan histopatologi jaringan luka dengan pengecatan Haematoxylin-Eosin. Skoring secara semikualitatif untuk menilai fibroblast dan kolagen. Hasil : Pada kelompok ekstrak pinang terdapat peningkatan level kolagen, akan tetapi tidak terjadi peningkatan level fibroblast pada hasil histopatologi luka kulit hari ke-7. Kesimpulan : terjadi peningkatan level kolagen pada pemberian ekstrak biji pinang. Kata kunci : biji pinang, luka, histopatologi, fibroblast, kolagen

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Accelerative Effect of Cinnamon Nanoparticles as well as HAMLET on Healing of Wounds Infected with MRSA in Diabetic Rats

BioMed Research International ◽

10.1155/2021/9984540 ◽

2021 ◽

Vol 2021 ◽

pp. 1-15

Author(s):

Ramezani Ali ◽

Najafpour Alireza ◽

Farahpour Mohammad Reza ◽

Mohammadi Rahim

Keyword(s):

Diabetic Rats ◽

Male Rats ◽

Control Group ◽

Wound Area ◽

Sterile Saline ◽

Area Reduction ◽

Significant Difference ◽

Healing Of Wounds ◽

Polymerase Chain ◽

Diabetic Wounds

Objective. The aim of the present study was to investigate the effect of cinnamon nanoparticles (CNPs) on healing of wounds infected with methicillin-resistant Staphylococcus aurous with human alpha-lactalbumin made lethal to tumor cells sensitization in diabetic rats. Methods. We included fifty diabetic male rats and divided them into 5 groups. There were 10 rats in each group as follows: CONTROL group: we did not infect the CONTROL group. The wound was only covered with sterile saline 0.9% solution (0.1 mL). INFCTD group: in this group, the wounds were infected with MRSA and covered with sterile saline 0.9% solution (0.1 mL). INFCTD-HMLT group: in this group, the wounds were infected with MRSA and HAMLET (100 μg). INFCTD-CNM group: in this group, the wounds were infected with MRSA and 0.1 mL CNPs (1 mg/mL) were applied topically to wounds. INFCTD-HMLT-CNM group: in this group, the wounds were infected with MRSA, HAMLET (100 μg), and 0.1 mL CNPs (1 mg/mL). Results. Bacteriology, wound area reduction measurements, biochemistry, histomorphometrical studies, hydroxyproline levels, and reverse transcription polymerase chain reaction for caspase-3, Bcl-2, and p53 showed significant difference between rats in the INFCTD-HMT-CNM group in comparison with other groups ( P < 0.05 ). Conclusions. Accelerated healing of diabetic wounds infected with MRSA showed that local application of cinnamon nanoparticles along with HAMLET sensitization on S. aureus-infected wound could be taken into consideration.

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PENGARUH LUMATAN DAUN PLETEKAN (Ruellia Tuberosa L) TERHADAP LAMA PENUTUPAN LUKA PADA TIKUS PUTIH (Rattus Novergicus)

Dunia Keperawatan ◽

10.20527/dk.v4i2.2513 ◽

2017 ◽

Vol 4 (2) ◽

pp. 104

Author(s):

Ria Susana ◽

Rismia Agustina ◽

Abdurahman Wahid

Keyword(s):

Wound Healing ◽

Treatment Group ◽

Random Sampling ◽

Wound Closure ◽

Simple Random Sampling ◽

Male Rats ◽

Control Group ◽

P Value ◽

Closure Time ◽

Significant Difference

ABSTRAKLuka didefinisikan sebagai gangguan pada fungsi dan integritas jaringan tubuh. Penyembuhan luka merupakan proses yang kompleks dari perbaikan dan remodeling jaringan sebagai respon terhadap cedera. Pletekan (ruellia tuberosa l) mengandung flavonoid, glikosida, phenol, saponin dan nutrisi yang diharapkan mempercepat penyembuhan luka. Tujuan penelitian ini adalah mengidentifikasi pengaruh lumatan daun pletekan (ruellia tuberosa l) terhadap lama penutupan luka pada tikus putih (rattus novergicus). Penelitian ini merupakan studi eksperimental dengan rancangan posttest only control, menggunakan teknik simple random sampling. Sampel yang digunakan sebanyak 40 ekor tikus jantan dengan luka insisi bersih sepanjang 3 cm yang dibagi menjadi 2 kelompok yaitu kelompok kontrol dan kelompok perlakuan. Data yang diukur adalah rata-rata lama penutupan luka. Data yang diperoleh dianalisis menggunakan uji mann-whitney dengan derajat kepercayaan 95% menunjukkan perbedaan yang signifikan rata-rata lama penutupan luka antara kelompok kontrol (8,45) dan kelompok perlakuan (3,75) dengan p-value 0,000 (p<0,05). Kesimpulan penelitian ini adalah terdapat pengaruh lumatan daun pletekan (ruellia tuberosa l) terhadap lama penutupan luka pada tikus putih (rattus novergicus).Kata-kata kunci: luka insisi bersih, pletekan (ruellia tuberosa l), lama penutupan luka.ABSTRACTWounds is defined as disruption of the function and integrity of body tissues. Wound healing isa complex process of tissue repair and remodelling in response to injury. Pletekan (ruellia tuberosa l) contains flavonoids, glycosides, phenols, saponins and nutritive value that suppose to accelerate wound healing. The purpose of this study was to identify the effect of pletekan leaves (ruellia tuberosa) to wound closure time in rats (rattus novergicus). This research was an experimental study with post- test design using the simple random sampling technique. The samples were 40 male rats with a clean incision wound along 3 cm which were divided into two groups, control group and treatment group. The measured data were the average of wound closure time. Data were analyzed using the mann- whitney test with 95 % confidence level showed a significant difference between the control group (8,45) and the treatment group (3,75) with p-value 0,000 (p<0,005). There was significant effect of ruellia tuberosa l to wound closure time in rattus novergicus.Keywords: wounds, ruellia tuberosa , wound closure time.

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The Effect of Snakehead Fish (Channa striata) Extract on Inflammation Reaction of Skin Wound Tissue in Rattus novergicus Wistar Strain

Berkala Ilmu Kesehatan Kulit dan Kelamin ◽

10.20473/bikk.v33.1.2021.48-54 ◽

2021 ◽

Vol 33 (1) ◽

pp. 48

Author(s):

Nanda Amalia Ramadhanti ◽

Willy Sandhika ◽

Agung Dwi Wahyu Widodo

Keyword(s):

Wound Healing ◽

Treatment Group ◽

Blood Vessels ◽

Inflammatory Reaction ◽

Skin Wound ◽

Male Rats ◽

Control Group ◽

Significant Difference ◽

Group 2 ◽

Wound Tissue

Background: Snakehead fish has been associated with wound healing due to its high albumin content. Albumin can accelerate the inflammatory process so that tissue repair will be faster. Lack of albumin protein causes a person tend to experience prolonged wound healing. Purpose: This study aims to prove the effect of snakehead fish extract on the number of macrophages and blood vessels on the inflammatory reaction of skin wound tissue in rats. Methods: Laboratory experimental design with a total sample size of 27 male rats have undergone incisions, divided into 3 random groups: control group was given aquadest, treatment group 1was given aquadest and 50% extract of snakehead fish, and treatment group 2 was given 100% extract of snakehead fish. The rat’s skin wound tissues were taken on day 5 and observed under the microscope. The average number of macrophages and blood vessels on granulation tissue that formed at the base of the wound were counted and then performed data analysis. Result: In this study, we found a down wards trend in the average number of macrophages and blood vessels in treatment group 1 and treatment group 2. The control group and the treatment group 2 showed a significant difference, however, between the other groups did not show significant difference. Conclusion: There is a significant effect of giving 100% snakehead fish extract on decreased macrophages and blood vessels in the inflammatory reaction of skin wound tissue.

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An improvement in acute wound healing in rats by the synergistic effect of photobiomodulation and arginine

Laboratory Animal Research ◽

10.1186/s42826-019-0025-x ◽

2019 ◽

Vol 35 (1) ◽

Cited By ~ 1

Author(s):

Atarodsadat Mostafavinia ◽

Mohammad Bidram ◽

Amirhossein Gomi Avili ◽

Mohammadamin Mahmanzar ◽

Seyed Ali Karimifard ◽

...

Keyword(s):

Wound Healing ◽

Pilot Study ◽

Main Phase ◽

Area Measurement ◽

Control Group ◽

Wound Area ◽

Treatment Regimens ◽

Wound Model ◽

Excisional Wound ◽

The Impact

AbstractIn this probe, at first we examined the best route and dosage of arginine administration on wound healing in an excisional wound model in rats. Next, we intend to assess the impact of photobiomodulation (PBM) and arginine, individually and together, on the wound healing. In the pilot study, an excisional wound was made in each of 24 rats. There were 4 groups. Group 1 was the control group. In groups 2 and 3, wounds were topically treated with arginine ointments (ARG.) 2% and 5%, respectively. In group 4, arginine was injected (ARG. INJ.,i.p.). In the main phase, in 24 new rats, an excisional wound was made. There were 4 groups: group 5 served as the control. Wounds in group 6 were topically treated with ARG 2%. Wounds in group 7 were subjected to PBM. Wounds in group 8 were treated with PBM+ARG. 2%. On day 15, wound area measurement, wound strength, and stereological examination were performed. In the pilot study, we found that the ARG 2% ointment significantly decreased wound area than ARG. 5%, ARG. INJ. and control groups, and significantly increased wound strength compared to the control and ARG.5% groups. In the main phase, a significant decrease of wound area in all treatment regimens was induced. PBM + ARG. 2% and PBM treatment regimens significantly improved wound strength and almost all stereological parameters, compared to the control and ARG. 2% groups. PBM + ARG. 2% induced anti-inflammatory and angiogenic activities, and hastened the wound healing process in an excisional wound model in rats.

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Effect of Subcutaneous Topical Ozone Therapy on Second-Degree Burn Wounds in Rats: An Experimental Study

Journal of Burn Care & Research ◽

10.1093/jbcr/irab110 ◽

2021 ◽

Author(s):

Emre Karakaya ◽

Aydincan Akdur ◽

Ebru Ayvazoğlu Soy ◽

Coşkun Araz ◽

Alev Ok Atilgan ◽

...

Keyword(s):

Wound Healing ◽

Healing Process ◽

Silver Sulfadiazine ◽

Male Rats ◽

Control Group ◽

Ozone Therapy ◽

Burn Wounds ◽

Degree Burn ◽

Significant Difference ◽

Second Degree

Abstract Burns are one of the most severe traumas, causing coagulative destruction of the skin. The use of various products that accelerate wound healing in patients with burns may affect rates of patient survival and reduce complications. We studied the effects of subcutaneous ozone injection on second-degree burn wounds in animal model. For this study, 72 Sprague-Dawley male rats were divided randomly into the following three groups: control group , silver sulfadiazine group, and ozone group; each group was then divided randomly into two subgroups (day 7 or day 14 examination and sacrifice). Superficial partial thickness burns were created on the lower back. In the control group, subcutaneous 0.9% serum saline was injected daily into the burn area. In the silver sulfadiazine group, burns were dressed daily with silver sulfadiazine. In the ozone group, subcutaneous ozone was injected daily into the burn area. We performed tissue hydroxyproline level measurements and histopathological evaluations. When groups were compared in terms of weight change, no significant difference was found between day 7 and day 14. With regard to tissue hydroxyproline levels the ozone group had significantly higher levels on both day 7 and day 14 (P < .001). In histopathological evaluations, we determined that wound healing in the ozone group was significantly higher than in the other groups. We found that subcutaneous ozone therapy was more effective than silver sulfadiazine in the healing process of second-degree burn wounds and could be safely used in the treatment of burn wounds.

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