scholarly journals Wound-healing effect of Platycodon grandiflorus (Jacq.) extract in rats

2021 ◽  
Vol 18 (9) ◽  
pp. 1909-1912
Author(s):  
Liang Wang ◽  
Li Yang ◽  
Gang Yuan ◽  
Long-Jiao Hu
Keyword(s):  
Wound Healing ◽  
Treated Group ◽  
Control Group ◽  
Type Iii Collagen ◽  
Matrix Metalloprotease ◽  
Wound Area ◽  
Burn Wounds ◽  
Type Iii ◽  
Healing Effect ◽  
Wound Tissue

Purpose: To evaluate the healing effect of Platycodon grandiflorus (Jacq.). extract (PGE) on experimental burn wounds in rats. Methods: Rats were randomly divided into four groups of eight rats each: control group, silver sulfadiazine (SSD)-treated group, moist exposed burn ointment (MEBO)-treated group and PGE-treated group. PGE, SSD and MEBO were applied topically twice daily for 7 days. SSD and MEBO were used as reference control. External observation of wound area contraction and histological analysis of wound tissues was performed respectively. The effect of PGE on matrix metalloproteases (MMPs), vascular endothelial growth factor (VEGF) and Type-III Collagen proteins of wound tissue in rats were analysed by Western blot. Results: After 10 days of topical treatment with PGE, PGE-treated group showed faster reduction in wound area when compared with control groups (p < 0.01). Matrix metalloprotease-2 (MMP-2), matrix metalloprotease-9 (MMP-9), VEGF and type-III collagen expressions in the wound tissue increased significantly (p < 0.05) when compared with the control burn wounds. Histological results showed an overall early recovery and regeneration in PGE-treated group when compared with control group. Conclusion: PGE possesses a significant wound-healing activity in full-thickness burn wounds in rats. Therefore, it can potentially be developed for the management of burns.

scholarly journals Effect of Zaoxiu ointment on wound-healing in experimental second degree burns rats

2021 ◽  
Vol 18 (9) ◽  
pp. 1913-1917
Author(s):  
Xiong Wang ◽  
Li Ma ◽  
Meng-ling Huang ◽  
Yong-gang Chen ◽  
Jin-hu Wu
Keyword(s):  
Wound Healing ◽  
Treated Group ◽  
Control Group ◽  
Type Iii Collagen ◽  
Early Recovery ◽  
Wound Area ◽  
Burn Wounds ◽  
Type Iii ◽  
Histological Results ◽  
Wound Tissue

Purpose: To evaluate the healing efficacy of a Traditional Chinese Medicine (TCM) preparation Zaoxiu Burn Ointment (ZBO) on experimental burns in rats. Methods: The rats were randomly divided into four groups of eight rats each: control group, silver sulfadiazine (SSD)-treated group, moist exposed burn ointment (MEBO)-treated group and ZBO-treated group. ZBO, SSD and MEBO were applied topically twice daily for 7 days. SSD and MEBO were used as reference control. The observation of wound area contraction and histological analysis of wound tissues were performed. The effect of ZBO on MMPs, VEGF and Type-III collagen proteins of wound tissue in rats were determined by Western blot. Results: After 10 days of topical treatment with ZBO, ZBO-treated group showed faster reduction in wound area in comparison with control groups (p < 0.01). MMP-2, MMP-9, VEGF and type-III collagen expression of the wound tissue increased significantly (p < 0.05) in comparison with the control burn wounds in rats. The ZBO had no cytotoxic effect on BHK-21 cell line. Histological results showed an overall early recovery and regeneration in ZBO-treated group when compared with control group. Conclusion: ZBO possesses significant wound-healing activity in full-thickness burn wounds in rats, and can potentially be developed for the treatment of burns patient in future.

scholarly journals Investigation of the bischofite gel reparative effect on the linear wound model

2018 ◽  
Vol 4 (3) ◽  
pp. 87-93 ◽  
Author(s):  
Alena Mayorova ◽  
Boris Sysuev ◽  
Isita Kchanaliyeva ◽  
Ekaterina Tretyakova ◽  
Snezhana Evseeva ◽  
...  
Keyword(s):  
Wound Healing ◽  
Male Rats ◽  
Control Group ◽  
Calorimetric Analysis ◽  
Wound Area ◽  
Healing Effect ◽  
Wound Model ◽  
Significant Difference ◽  
Morphological Picture ◽  
Reparative Effect

Introduction. The currently available information on the bischofite mineral pharmacodynamics makes it possible to assume that bischofite-based gels have wound-healing properties. Materials and methods. Experiments were performed on 36 male rats. Using a blade, a linear wound of 50 mm long was modeled. Animals were divided into 4 groups: 1) Control; 2) Bishofit (500 mg of gel with bischofite on the wound area for 7 days); 3) Actovegine (500 mg of Actovegine gel on the wound area for 7 days); 4) Contractubex (500 mg of contractubex gel on the wound area for 7 days). The wound healing effect of the drugs was evaluated through studying the physicomechanical properties, assessing the morphological picture, determining the concentration of hydroxyproline (HP) and calculating the ratio of types I and III collagen. Results. The strongest healing effect on the wound defect, significantly greater than that in the control (p&lt;0.01), was obtained in the group that received bischofite gel (13.70±0.76 N). Actovegine also demonstrated a positive reparative effect (12.60±0.63 N, p&lt;0.05). Significantly lower (p&lt;0.01) healing effect than in the control group was obtained in the group of animals that had received contractubex (9.65±0.59 N). In the calorimetric analysis, it was found that the highest concentration of HP was in the tissues of wound defects in animals treated with contractubex; however, there was no statistically significant difference with the control group. Significantly lower in comparison with the control (p&lt;0.05) concentration of HP, was found in tissues of simulated wounds in animals treated with bischofite gel (79.7% of the control). When assessing the ratio of types I and III collagen in the tissues of the wound defect, when stained with picrosirius red, it was found that by the number of mature collagen fibers, the studied groups can be arranged in the following descending order: Bishofite&gt; Actovegine&gt; Control&gt; Contractubex. A similar trend is demonstrated by the morphological picture of tissues in the area of the wound defect. Conclusion. The study showed that the best results were obtained with external use of bischofite gel. Actovegine has a less significant, but quite pronounced reparative, effect on this model. The least satisfactory results were obtained when applying contractubex.

scholarly journals Wound Healing Effect of Nonthermal Atmospheric Pressure Plasma Jet on a Rat Burn Wound Model: A Preliminary Study

2019 ◽  
Vol 40 (6) ◽  
pp. 923-929 ◽  
Author(s):  
Yoonje Lee ◽  
Sanjaya Ricky ◽  
Tae Ho Lim ◽  
Ki-Seok Jang ◽  
Hongjung Kim ◽  
...  
Keyword(s):  
Wound Healing ◽  
Atmospheric Pressure ◽  
Atmospheric Pressure Plasma ◽  
Plasma Jet ◽  
Treated Group ◽  
Control Group ◽  
Atmospheric Pressure Plasma Jet ◽  
Plasma Medicine ◽  
Burn Wound ◽  
Burn Wounds

Abstract Worldwide, an estimated 6 million patients seek medical attention for burns annually. Various treatment methods and materials have been investigated and developed to enhance burn wound healing. Recently, a new technology, plasma medicine, has emerged to offer new solutions in wound care. As the development of plasma medicine has shown benefit in wound healing, we aimed to assess the effects of plasma medicine on burn wounds. To investigate the effectiveness of a nonthermal atmospheric pressure plasma jet (NAPPJ) for burn wound treatment on a brass comb burn wound rat model. Burn wounds were made by applying a preheated brass comb (100°C) for 2 minutes, which resulted in four full-thickness burn wounds separated by three interspaces. Interspaces were exposed to NAPPJ treatment for 2 minutes and morphological changes and neutrophil infiltration were monitored at 0, 4, and 7 days post-wounding. The percentage of necrotic interspace was higher in the control group than in the plasma-treated group (51.8 ± 20.5% vs 31.5 ± 19.0%, P &lt; .001). Moreover, the exposure of interspace to NAPPJ greatly reduced the number of infiltrating neutrophils. In addition, the percentage of interspace that underwent full-thickness necrosis in the plasma-treated group was smaller than that in the control group (28% vs 67%). NAPPJ exposure on interspaces has a positive effect on burn wounds leading to wound healing by reducing burn injury progression.

scholarly journals Wound Healing Study of Panchavalkal Ointment in Wistar Albino Rats

2021 ◽  
pp. 110-116
Author(s):  
Vaikhari Dhurve ◽  
Pravin Jawanjal ◽  
Mukesh Naria ◽  
Tukaram Dudhamal ◽  
Minal Kalambe ◽  
...  
Keyword(s):  
Wound Healing ◽  
Treated Group ◽  
Control Group ◽  
Albino Rats ◽  
Wound Area ◽  
Mean Values ◽  
Wistar Strain ◽  
The Mean ◽  
Wistar Albino Rats ◽  
Wound Healing Activity

Introduction: Panchavalkal is a well-known Ayurvedic poly-herbal formulation that has been reported to be used against inflammation, to clean ulcer, wound. Aims and Objectives: To investigate the wound healing activity of Panchavalkal ointment. Materials and Methods: Wistar strain albino rats of either sex weighing 200±20 g were used for the experiments divided in four groups each consisted of six rats.  Statistical Analysis: One-way analysis of variance (ANOVA) was used to compare the mean values of quantitative variables among the groups followed by Dunnett’s multiple ‘t’ test Observations & Results: Sesame oil and Panchvalkal ointment showed almost similar wound healing effects in comparison to control group. Discussion: Panchavalkal ointment showed statistically highly significant percentage of contraction of excision wound area compared to the normal control. Epithelization period was significantly decreased in oil and Panchavalkal ointment treated group. Conclusion: Panchavalkal ointment decreased the pain, tenderness, redness and swelling that helps to control infection and enhanced the rate of wound healing in albino rats.

scholarly journals Effectiveness of maggot extractions and secretion (E/S) of Lucilia sericata in reducing wound surface in experimental scalding burn injury

2020 ◽  
Vol 13 (1) ◽  
Author(s):  
Zahra Sadat Amiri ◽  
Kamran Akbarzadeh ◽  
Masoumeh Douraghi ◽  
Khosrou M Abdi ◽  
Abbas Aghaei Afshar ◽  
...  
Keyword(s):  
Wound Healing ◽  
Burn Injury ◽  
Intervention Group ◽  
Recovery Process ◽  
Treated Group ◽  
Lucilia Sericata ◽  
Control Group ◽  
Therapeutic Effects ◽  
Wound Surface ◽  
Burn Wounds

Abstract. Amiri ZS, Akbarzadeh K, Douraghi M, Abdi KM, Afshar AA, Ghaffari J, Kordshouli RS, Akbari M, Rafinejad J. 2021. Effectiveness of maggot extractions and secretion (E/S) of Lucilia sericata in reducing wound surface in an experimental scalding burn injury. Nusantara Bioscience 13: 11-15. Lucilia sericata larvae have been successfully used as medicinal larvae for wound healing. This study was performed to evaluate the therapeutic effects of the primary ointment made from excretory-secretory substances of L.sericata larvae on experimental burn wounds on rabbits under laboratory conditions. Extraction of ES from the third stage of L.sericata larvae and antibiogram testing was done. Four rabbits were randomly divided into four groups; three standard third-grade burn wounds were created on the back of each rabbit for intervention groups (B, D) of wounds were used respectively from the original ointment made from ES and ES without accompanying material. Nitrofurazone ointment was used for the positive (C) and placebo ointment was used for the negative (A). On the 21st day, all scars of wounds in groups B and D were separated and the wound was free of infectious tissue, whereas in groups A and C that the scars were clinging to the base. The level of the ulcer was significantly lower in the E/S intervention group and the infectious tissue was not observed during the treatment of group B and D wounds. Methicillin-resistant Staphylococcus aureus (MRSA) did not show resistance to excretory-secretory substances of L. sericata larvae. The debridement and recovery process was faster in the ES-treated group and the initial ointment than in the control group. The results showed that maggot extractions and secretion (E/S) of L. sericata in reducing wound healing is appropriate for treatment.

scholarly journals Wound Healing Potential of Crocin and Safranal, Main Saffron (Crocus sativus L.), the Active Constituents in Excision Wound Model in Rats

2021 ◽  
Vol 10 ◽  
Author(s):  
Nafise Deldar ◽  
Malihezaman Monsefi ◽  
Mohsen Salmanpour ◽  
Mohadeseh Ostovar ◽  
Mojtaba Heydari
Keyword(s):  
Wound Healing ◽  
Crocus Sativus ◽  
Control Group ◽  
Topical Formulation ◽  
Adult Rats ◽  
Wound Area ◽  
Active Constituents ◽  
Healing Effect ◽  
Wound Model ◽  
Wound Healing Effect

Background: Saffron is traditionally suggested for wound healing in Persian medicine. It is investigated for wound healing effect in multiple studies with promising results. It is not examined that which ingredient of saffron contributes more to this effect. This study was aimed to evaluate and compare the wound healing potential of saffron and its active constituents, crocin, and safranal, in rats. Materials and Methods: Forty female adult rats with induced excision wounds were randomly divided into four groups to receive topical formulation of saffron, crocin, safranal, and placebo for seven days. The Wound area and histopathologic stage of wound healing were evaluated as outcome measures. Results: The wound area was significantly lower in treatment groups (saffron, crocin, and safranal) compared to the control group on day 7 of the intervention. Compared to the control group, the wound in all treated groups showed a decreased inflammatory response and more progression to the proliferation phase. The saffron group showed more advanced healing phase with complete epithelialization of the wound on day 7 of study when partial and no epithelialization was observed in safranal and crocin groups. Conclusion: The study showed the wound healing properties of both safranal and crocin with the slight superiority of safranal. However, saffron seems to be more potent in wound healing effect compared to safranal and crocin as its main active constituents. [GMJ.2021;10:e1900]

scholarly journals Healing Effects of Iranian Dwarf Elder on Full-thickness Epidermal Thermal Injury in Wistar Rats

2022 ◽  
Vol 19 (4) ◽  
Author(s):  
Hosein Mohamadi haftador ◽  
Parisa Ramhormozi ◽  
Mitra Yousefpour ◽  
Ali Sobhanizadeh ◽  
Laya Ghahari
Keyword(s):  
Wound Healing ◽  
Treatment Group ◽  
Wistar Rats ◽  
Thermal Injury ◽  
Herbal Medicines ◽  
Vehicle Group ◽  
Control Group ◽  
Full Thickness ◽  
Wound Area ◽  
Burn Wounds

Background: Wound healing is a physiologic process that cells and tissues react when the skin is exposed to thermal injury. The inflammatory process has ‎essential effects on wound healing that includes coordination between immunological and biological responses. Chemical and herbal medicines are locally or systemically administrated to help wound healing. Herbal medicines have become more prevalent in recent years due to fewer side effects. Dwarf elder is used to treating inflammation and has been reported to possess antibacterial and antioxidant activities. This plant is also effective against burns, wounds, eczema, and infection. Objectives: In this study, we evaluated the topical application of dwarf elder on full-thickness epidermal thermal wounds in Wistar rats. Methods: Thirty adult male Wistar rats of about 300 g were used. The rats were randomly divided into three groups: Control group, vehicle group treated with an ointment base, and treatment group treated with elder ointment 10%. The animals were anesthetized and were created burn wounds with an area of 1 cm2 using the Meyer method. After 21 days, the wounds were photographed with a Canon camera. The wound area and healing degree were calculated. Slides were stained with H&E and examined by light microscopy and GraphPad Prism software. Data were analyzed by SPSS software using ANOVA and Tukey’s test. The significance level was considered at P < 0.05. Results: The wound area on the 21st day was significantly lower in the treatment group than in the control and vehicle groups. The epithelium thickness (μm) was significantly higher in the treatment group than in the control and vehicle groups. The blood vessels were significantly higher in the treatment group than in the other groups. Conclusions: The study showed that elder extract could be an effective remedy to repair and heal thermal injury and burn wounds.

Ethanol Extract of Achillea fragrantissima Enhances Angiogenesis through Stimulation of VEGF Production

2020 ◽  
Vol 21 ◽  
Author(s):  
Hana M. Hammad ◽  
Amer Imraish ◽  
Maysa Al-Hussaini ◽  
Malek Zihlif ◽  
Amani A. Harb ◽  
...  
Keyword(s):  
Wound Healing ◽  
Rural Communities ◽  
Ex Vivo ◽  
Ethanol Extract ◽  
Aortic Ring ◽  
Treated Group ◽  
Control Group ◽  
Dependent Manner ◽  
Achillea Fragrantissima

Objective: Achillea fragrantissima L. (Asteraceae) is a traditionally used medicinal herb in the rural communities of Jordan. Methods: The present study evaluated the efficacy of the ethanol extract of this species on angiogenesis in both, ex vivo using rat aortic ring assay and in vivo using rat excision wound model. Results: In concentrations of 50 and 100 µg/ml, the ethanol extract showed angiogenic stimulatory effect and significantly increased length of capillary protrusions around aorta rings of about 60% in comparison to those of untreated aorta rings. In MCF-7 cells, the ethanol extract of A. fragrantissima stimulates the production of VEGF in a dose-dependent manner. 1% and 5% of ethanol extract of A. fragrantissima containing vaseline based ointment was applied on rat excision wounds for six days and was found to be effective in wound healing and maturation of the scar. Both preparations resulted in better wound healing when compared to the untreated control group and vaseline-treated group. This effect was comparable to that induced by MEBO, the positive control. Conclusion: The results indicate that A. fragrantissima has a pro-angiogenic effect, which may act through the VEGF signaling pathway.

Cold Plasma Treatment Promotes Full-thickness Healing of Skin Wounds in Murine Models

2021 ◽  
pp. 153473462110021
Author(s):  
Joon M. Jung ◽  
Hae K. Yoon ◽  
Chang J. Jung ◽  
Soo Y. Jo ◽  
Sang G. Hwang ◽  
...  
Keyword(s):  
Wound Healing ◽  
Plasma Treatment ◽  
Cold Plasma ◽  
Smooth Muscle Actin ◽  
Treated Group ◽  
Control Group ◽  
Alpha Smooth Muscle Actin ◽  
Skin Wound Healing

Cold plasma can be beneficial for promoting skin wound healing and has a high potential of being effectively used in treating various wounds. Our aim was to verify the effect of cold plasma in accelerating wound healing and investigate its underlying mechanism in vitro and in vivo. For the in vivo experiments, 2 full-thickness dermal wounds were created in each mouse (n = 30). While one wound was exposed to 2 daily plasma treatments for 3 min, the other wound served as a control. The wounds were evaluated by imaging and histological analyses at 4, 7, and 11 days post the wound infliction process. Immunohistochemical studies were also performed at the same time points. In vitro proliferation and scratch assay using HaCaT keratinocytes and fibroblasts were performed. The expression levels of wound healing–related genes were analyzed by real-time polymerase chain reaction and western blot analysis. On day 7, the wound healing rates were 53.94% and 63.58% for the control group and the plasma-treated group, respectively. On day 11, these rates were 76.05% and 93.44% for the control and plasma-treated groups, respectively, and the difference between them was significant ( P = .039). Histological analysis demonstrated that plasma treatment promotes the formation of epidermal keratin and granular layers. Immunohistochemical studies also revealed that collagen 1, collagen 3, and alpha-smooth muscle actin appeared more abundantly in the plasma-treated group than in the control group. In vitro, the proliferation of keratinocytes was promoted by plasma exposure. Scratch assay showed that fibroblast exposure to plasma increased their migration. The expression levels of collagen 1, collagen 3, and alpha-smooth muscle actin were elevated upon plasma treatment. In conclusion, cold plasma can accelerate skin wound healing and is well tolerated.

673 Effect of Subcutaneous Topical Ozone Therapy on Second Degree Burn Wounds in Rats: An Experimental Study

2021 ◽  
Vol 42 (Supplement_1) ◽  
pp. S193-S193
Author(s):  
Emre Karakaya ◽  
Aydincan Akdur ◽  
H Ebru ◽  
Ayvazoglu Soy ◽  
Alev Ok Atilgan ◽  
...  
Keyword(s):  
Wound Healing ◽  
Healing Process ◽  
Silver Sulfadiazine ◽  
Male Rats ◽  
Control Group ◽  
Ozone Therapy ◽  
Burn Wounds ◽  
Degree Burn ◽  
Significant Difference ◽  
Second Degree

Abstract Introduction Burn is one of the most severe traumas that causes coagulative destruction of the skin. The use of various products that accelerate wound healing in patients with burn may affect the patient’s survival and reduce the complications that may be seen. In the present study we aimed effects of subcutaneous ozone injection on second degree burn wound. Methods A total of 72 Sprague-Dawley male rats included in the study were divided randomly into three groups (control group (CG), silver sulfadiazine group (SG), ozone group (OG)) and each group was divided randomly two subgroups (as sacrificed on d7 and on d14).A deep second degree scald burns were created on the lower back. In CG subcutaneous 0.9% serum saline was injected daily into the burn area. In SG, burns were dressed with silver sulfadiazine daily and in OG subcutaneous ozone was injected daily into the burn area. Tissue hydroxyproline level measurement and histopathological evaluation were done. Results When the groups were compared in terms of weight change, no significant difference was found on the 7th and 14th days. In the evaluation made in terms of tissue hydroxyproline, tissue hydroxyproline level in OG was found to be significantly higher on both the 7th and 14th days (p &lt; 0.001). In histopathological evaluations, it was determined that wound healing in OG was significantly higher than in the other groups. Conclusions According to the results, subcutaneous ozone therapy is more effective than silver sulphadiazine in the healing process of second-degree burn wounds and it can be safely used in the treatment of burn wounds.

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