scholarly journals Protease supplementation in maize-based diet influenced net energy and nutrient digestibility in broilers

Author(s):  
K.W. McCafferty ◽  
A.F. Moss ◽  
N.K. Morgan ◽  
A.J. Cowieson ◽  
M. Choct

This experiment evaluated the effects of supplemental protease in maize-based diets formulated with reduced density of digestible amino acids (dAA) on net energy (NE) utilisation and nutrient digestibility in broilers. A total of 312, one-day-old, male broilers (Ross 308) were randomly distributed into 24 floor pens and fed one of three treatments, with eight pen replicates per treatment throughout the starter (1 to 14 d of age) and grower (15 to 28 d of age) phases. Dietary treatments consisted of a positive control (PC) reference diet, a negative control diet (NC; dAA density 60 g/kg less than PC diet), and a NC diet with supplemental protease (200 mg/kg). All diets contained supplemental phytase (200 mg/kg) and xylanase (200 mg/kg). The reduction in dAA density between the PC and NC did not affect (P>0.05) NE, but protease supplementation in the NC diet increased (P<0.05) NE by 0.27 MJ/kg, compared with those receiving the NC diet without protease. The reduction in dAA for broilers fed the NC and PC diets did not (P>0.05) affect nitrogen, starch, or DE in the jejunum or ileum. Likewise, no differences (P>0.05) in jejunal (nitrogen, starch, and DE) and ileal (starch and DE) digestibility values were observed between those offered the NC diets without or with protease, but a small difference (P<0.05) in ileal nitrogen digestibility was observed.

1990 ◽  
Vol 38 (3B) ◽  
pp. 609-622
Author(s):  
N.P. Lenis ◽  
J.T.M. van Diepen

Individual and group housed crossbred pigs 45 to 105 kg and 65 to 95 kg in experiments 1 and 2, respectively, were given basal diets with L-threonine 0.6, 1.2 and 1.8 g/kg. Positive and negative control diets contained total threonine 5.7 and 4.5 g/kg, respectively. To prevent other amino acids being limiting, the negative control diet was supplemented with lysine, methionine, tryptophan, isoleucine, histidine and valine. The positive control diet was supplemented with lysine and methionine. The requirement for total threonine of growing-finishing pigs for maximum growth performance was about 5.6 g/kg in a diet containing net energy 9.4 MJ/kg. This figure corresponds with about 4.7 g/kg apparent faecal digestible threonine and 4.3 apparent ileal digestible threonine. There was no difference between the growing and the finishing pigs. The requirement for ileal digestible threonine, relative to ileal digestible lysine requirement, was about 64%. It is concluded that dietary protein can be reduced by 2 percentage units without any adverse effect on growth performance, if limiting amino acids are sufficiently supplemented. (Abstract retrieved from CAB Abstracts by CABI’s permission)


2019 ◽  
Vol 4 (1) ◽  
pp. 49-58
Author(s):  
Madie R Wensley ◽  
Jason C Woodworth ◽  
Joel M Derouchey ◽  
Steve S Dritz ◽  
Mike D Tokach ◽  
...  

Abstract Three experiments were conducted to determine the effect of three fermented amino acids (AA) with their respective biomass compared to crystalline AA on the growth performance of swine and poultry. In experiment 1, 315 barrows (DNA 200 × 400, initially 11.3 ± 0.69 kg) were allotted to 1 of 4 dietary treatments with 5 pigs per pen and 15 or 16 pens per treatment. Dietary treatments included a negative control (16% standardized ileal digestible [SID] Tryptophan:lysine [Trp:Lys] ratio), positive control (21% SID Trp:Lys ratio from crystalline Trp), or diets containing Trp with biomass to provide 21 or 23.5% SID Trp:Lys ratios, respectively. Pigs fed the positive control or low Trp with biomass diet had increased (P &lt; 0.05) ADG compared to pigs fed the negative control diet, with pigs fed the high Trp with biomass diet intermediate. Pigs fed the low Trp with biomass diet had increased (P &lt; 0.05) G:F compared to the negative control diet, with others intermediate. In experiment 2, 1,320 1-d-old male broilers (Cobb 500, initially 45.2 g) were allotted to one of four dietary treatments with 33 birds per pen and 10 pens per treatment. Dietary treatments included a negative control (58/58% Threonine:lysine [Thr:Lys] ratio), positive control (65/66% Thr:Lys ratio from crystalline Thr), or diets containing Thr with biomass to provide 65/66 or 69/70% Thr:Lys ratios in starter and grower diets, respectively. Broilers fed the positive control or Thr with biomass diets had increased (P &lt; 0.05) ADG compared to broilers fed the negative control diet. Broilers fed the positive control or the low Thr with biomass diet had increased (P &lt; 0.05) G:F compared to the negative control and high Thr with biomass treatments. In experiment 3, 2,100 one-day-old male broilers (Cobb 500, initially 39.4 g) were allotted to one of four dietary treatments with 35 birds per pen and 15 pens per treatment. Dietary treatments included a negative control (59/63% Valine:lysine [Val:Lys] ratio), positive control (75/76% Val:Lys ratio from crystalline Val), or diets containing Val with biomass to provide 75/76 or 84/83% Val:Lys ratios in starter and grower diets, respectively. Broilers fed the positive control or Val with biomass diets had increased (P &lt; 0.05) ADG, ADFI, and G:F compared to those fed the negative control diet. In conclusion, Trp, Thr, or Val with their respective biomass appear to be equally bioavailable and a suitable alternative to crystalline AA in swine and poultry diets.


2021 ◽  
pp. 1-12
Author(s):  
K.W. McCafferty ◽  
A.F. Moss ◽  
N.K. Morgan ◽  
A.J. Cowieson ◽  
M. Choct

A study was conducted to evaluate the effects of supplemental protease and cereal grain type on nutrient digestibility (jejunum and ileum) and performance of broilers offered diets with reduced amino acid concentrations and supplemental xylanase and phytase. A total of 624 male broiler chicks (Ross 308) were randomly distributed into 48 floor pens (13 chicks/pen; 0.07 m2/bird) and offered one of six dietary treatments with eight replicates per treatment. Dietary treatments were either maize- or wheat-based with a positive control (PC) reference diet, a negative control diet without protease (NC; 60 g/kg lower amino acid density than PC), and an NC diet with protease. The reduction in amino acid density affected (P<0.05) nutrient digestibility by varying degrees depending on cereal grain source. At 14 d of age, cereal grain and protease showed a significant interaction (P<0.05) which affected jejunal and ileal starch digestibility, whereby protease increased digestibility in birds fed wheat-based diets but not in those fed maize-based diets. Cereal grain source affected (P<0.05) nitrogen (jejunum and ileum) and digestible energy (DE; ileum), where birds fed wheat-based diets had higher digestibility than those fed maize-based diets. At 28 d of age, birds fed wheat-based diets had a higher (P<0.01) jejunal and ileal nitrogen digestibility, whereas protease reduced ileal nitrogen digestion. Protease affected ileal starch digestion in birds fed wheat, but not maize-based diets, resulting in a significant cereal grain × protease interaction (P<0.05). Wheat-based diets had a higher DE than maize-based diets in both the jejunum and ileum. From 15 to 35 d of age, cereal grain source (P<0.05) affected performance, whereby broilers offered maize-based diets had better performance than those fed wheat-based diets.


2019 ◽  
Vol 97 (8) ◽  
pp. 3390-3398 ◽  
Author(s):  
Laia Blavi ◽  
Cristhiam J Muñoz ◽  
Jonathan N Broomhead ◽  
Hans H Stein

AbstractTwo experiments were conducted to test the hypothesis that a corn-expressed phytase increases growth performance, bone measurements, and nutrient digestibility by young growing pigs, if added to diets that are deficient in Ca and P. In Exp. 1, 60 pigs (initial BW: 10.78 ± 0.67 kg) were randomly allotted to 6 dietary treatments that included a positive control diet (PC; 0.70% total Ca and 0.60% total P) and a negative control diet (NC; 0.50% total Ca and 0.42% total P). Four additional diets were formulated by supplementing the NC diet with 250, 500, 1,000, or 1,500 phytase units (FTU)/kg. Diets were fed for 28 d and the individual BW of pigs on days 1 and 28 were recorded. Fecal samples were collected from days 25 to 27 to calculate apparent total tract digestibility (ATTD) of Ca and P. On the last day of the experiment, all pigs were euthanized, and the left femur was removed and analyzed for ash, Ca, and P. Results indicated that growth performance, ATTD of Ca and P, and bone ash measurements were reduced (P < 0.05) in NC fed pigs compared with PC fed pigs. However, growth performance, ATTD of Ca and P, and bone ash measurements were improved (linear and quadratic, P < 0.05) by including increasing concentrations of phytase to the NC diet. In Exp. 2, experimental procedures were similar to those used in Exp. 1. Forty-eight pigs (initial BW: 11.15 ± 0.85 kg) were randomly allotted to 6 dietary treatments in a 28-d experiment. Treatments included a PC diet, an NC diet, and 4 diets in which 500 or 1,000 FTU/kg of either the corn-expressed phytase or a commercial microbial phytase were added to the NC diet. Pigs fed the NC diet had reduced (P < 0.01) final BW, ADG, G:F, and bone ash concentrations compared with pigs fed the PC diet. When 500 FTU/kg phytase was fed, no differences were observed in growth performance or bone ash measurements between phytase sources, and there were no differences in growth performance among pigs fed 1,000 FTU/kg of either phytase source or the PC diet. However, regardless of concentration or source of phytase, pigs fed the PC diet had greater (P < 0.001) amount of bone ash, bone Ca, and bone P compared with pigs fed phytase diets. In conclusion, the corn-expressed phytase is effective in improving growth performance, Ca and P digestibility, and bone measurements in pigs fed diets that are deficient in Ca and P.


1990 ◽  
Vol 38 (3B) ◽  
pp. 597-607
Author(s):  
J.B. Schutte ◽  
M.W. Bosch ◽  
N.P. Lenis ◽  
J. de Jong ◽  
J.T.M. van Diepen

In 6 trials with 460 pigs, initially about 19 kg the threonine requirement for growth between about 20 to 40 kg was investigated. To a negative control diet, containing crude protein 160 g and threonine 5.6 g/kg. L-threonine 0.0, 0.6, 1.2 and 1.8 g/kg, was added and compared with a positive control diet with crude protein 185 g/kg and threonine 6.8 g/kg. The negative control diet was supplemented with lysine, methionine, tryptophan, isoleucine, histidine and valine so that no other amino acids than threonine were limiting. The positive control diet was supplemented with lysine and methionine. The experimental diets were fed ad libitum as pellets. In 2 separate trials, the apparent faecal and ileal digestibility of the amino acids of the 2 control diets was estimated. The requirement for total threonine was 7.1 g/kg for maximum weight gain, and 7.4 g/kg for maximum efficiency of feed utilization in a diet containing net energy 2290 kcal/kg. These results correspond with 6.2 and 6.5 g/kg faecal digestible and 5.7 and 6.0 g/kg ileal digestible threonine, respectively. Pig performance on the negative control diet supplemented with the first limiting amino acids, was similar to that of the positive control diet. (Abstract retrieved from CAB Abstracts by CABI’s permission)


Author(s):  
D. Wu ◽  
M. Choct ◽  
S. B. Wu ◽  
Y. G. Liu ◽  
R. A. Swick

SummaryA study was conducted to examine the effects of a multi-carbohydrase enzyme complex on the nutritive value of wheat in diets differing in nutrient density. It was hypothesised that response to enzyme inclusion would be greater in diets with lower nutrient density. The study was conducted using 1008 Ross 308 male broiler chicks (four treatments with seven replicate pens of 36 chicks). A 2 × 2 factorial arrangement of treatments was employed. Factors were adequate or low nutrient density with or without enzyme supplementation. The wheat-soybean meal based positive control (PC) diet was formulated to be nutritionally adequate in energy and digestible amino acids according to local industry recommendations. A negative control (NC) was formulated to have 80 kcal/kg less ME and 1.5% less digestible amino acids as compared to the PC. A multi-carbohydrase complex containing 19 carbohydrase activities derived from Penicillium funiculosum was added in both the PC and NC diets (Rovabio® Excel LC, Adisseo Asia Pacific Pte Ltd., Singapore). Birds fed the NC had 3.7 points (P < 0.05) poorer FCR than the PC. Across the diet type, enzyme supplementation increased body weight by 3.2% (P < 0.05) and improved FCR by 5.2 points (P < 0.01). There was no nutrient density x enzyme interaction (P > 0.05), indicating that performance improvement was independent of nutrient density. Apparent ileal digestibility of crude protein followed a similar trend, showing a 4.9% enhancement (P < 0.01) with the inclusion of the enzyme product in either diet. Enzyme supplementation reduced ileal viscosity by 39.0% (P < 0.05). It was concluded that multi-carbohydrase could overcome the negative effect in broiler performance brought by nutrient reduction, however, there was no indication that nutrient density affected bird response to supplementation of multi-carbohydrase.


2019 ◽  
Vol 40 (2) ◽  
pp. 767
Author(s):  
Gabriel Villela Dessimoni ◽  
Nilva Kazue Sakomura ◽  
Daniella Carolina Zanardo Donato ◽  
Fábio Goldflus ◽  
Nayara Tavares Ferreira ◽  
...  

Although phytase has been researched, new enzymes have been produced, leading to different animal responses. In this scenario, the present study proposes to evaluate the inclusion of a bacterial phytase produced by Escherichia coli in broiler diets based on corn and soybean meal, with or without nutrient reductions, on the performance, nutrient digestibility, phosphorus bioavailability, and bone minerals of those animals and on the economic viability of this practice. A total of 896 male broiler chickens were distributed into 32 experimental units, each housing 28 broilers. The experiment was set up as a completely randomized design with four treatments (Positive Control (PC)- diet meeting the nutritional requirements of the broiler chickens; Negative Control (NC) with reductions of 100kcal/kg of ME, 0.14% avP and 0.11% tCa; NC + phytase (500 FTU/kg); PC + phytase (500FTU/kg)) and 8 replicates. Phytase increased (p < 0.05) feed intake and body weight gain and improved feed conversion ratio in starter (1 to 21 days) and total (1 to 42 days) phases, respectively, compared with Negative Control diet without supplementation. The Negative Control + phytase diet also led to a feed intake similar to Positive Control in the starter and total phases. The inclusion of phytase without nutrient reductions improved (p < 0.05) feed conversion ratio in the starter phase, compared to Positive Control diet. There was an increase (p < 0.05) in the digestibility of dry matter, crude protein and phosphorus and in apparent digestible energy in the Negative Control + phytase diet compared to the Negative Control diet. Phytase supplementation increased (p < 0.05) the digestibility of crude protein, calcium and phosphorus, and apparent digestible energy compared to Positive Control diet. Ash, phosphorus, and calcium contents were higher in the Negative Control + phytase diet compared with those observed in the Negative Control diet without enzyme (p < 0.05). The Positive Control + phytase diet provided higher ash contents (p < 0.05), but calcium and phosphorus deposition was similar to those obtained with Positive Control diet. Phytase inclusion allows for a reduction in the diet cost per ton of produced feed. In conclusion, dietary supplementation with bacterial phytase produced from Escherichia coli for broiler chickens is recommended, as it provided increases in production performance, nutrient digestibility, and energy metabolizability and a reduction in the diet cost.


2020 ◽  
Vol 4 (4) ◽  
Author(s):  
Zhong-Xing Rao ◽  
Mike D Tokach ◽  
Steve S Dritz ◽  
Jason C Woodworth ◽  
Joel M DeRouchey ◽  
...  

Abstract Two experiments were conducted to determine the efficacy of various commercial products on growth performance of nursery pigs fed diets high in fumonisin. In experiment 1, 350 pigs (241 × 600; DNA, Columbus, NE; initially 9.9 kg) were used with five pigs per pen and 14 replicates per treatment. After weaning, pigs were fed common diets for 21 d before the experiment started. The five dietary treatments consisted of a positive control (low fumonisin), a negative control (60 mg/kg of fumonisin B1 + B2 in complete diet), and the negative control with one of three products (0.3% of Kallsil Dry, Kemin Industries Inc., Des Moines, IA; 0.3% of Feed Aid Wide Spectrum, NutriQuest, Mason City, IA; 0.17% of Biofix Select Pro, Biomin America Inc., Overland Park, KS). Diets were fed in mash form for 14 d and followed with a low fumonisin diet for 13 d. For the 14-d treatment period, pigs fed the positive control diet and Biofix Select Pro had greater (P &lt; 0.05) average daily gain (ADG), average daily feed intake (ADFI), and gain:feed (G:F) compared to those fed the high fumonisin negative control, or high fumonisin diets with Kallsil Dry or Feed Aid Wide Spectrum. Serum sphinganine to sphingosine ratios (SA:SO) were greater (P &lt; 0.05) in all pigs fed high fumonisin diets compared to the positive control. In experiment 2, 300 pigs (241 × 600; DNA; initially 10.4 kg) were used. Procedures were similar to experiment 1 except there were 12 replicate pens per treatment, high fumonisin diets contained 30 mg/kg fumonisin, and experimental diets were fed for 28 d. Similar to experiment 1, pigs fed the positive control diet and treatment with Biofix Select Pro had greater (P &lt; 0.05) ADG and G:F, and lower (P &lt; 0.05) serum SA:SO compared to pigs fed the high fumonisin negative control, or high fumonisin diets with Kallsil Dry or Feed Aid Wide Spectrum. In summary, pigs fed diets containing 60 mg/kg of fumonisin for 14 d or 30 mg/kg of fumonisin for 28 d had poorer ADG and G:F and greater serum SA:SO compared to pigs fed a diet with less than 5 mg/kg of fumonisin. Adding Biofix Select Pro to diets appeared to mitigate the negative effects of high fumonisin concentrations, while Kallsil Dry and Feed Aid Wide Spectrum did not.


2021 ◽  
Vol 99 (Supplement_1) ◽  
pp. 48-49
Author(s):  
Jung Wook Lee ◽  
Tyler Chevalier ◽  
Chris Sparks ◽  
Tom D Crenshaw ◽  
Merlin D Lindemann

Abstract A 36-d experiment evaluated effects of supplemental OptiPhos Plus phytase (Huvepharma, Peachtree City, GA) on growth, bone mineralization, Ca and P digestibility of nursery-grower pigs. Individually housed crossbred pigs (n = 42; initial body weight [BW], 16.1 ± 0.4 kg) were randomly allotted to 7 dietary treatments based on BW and sex. A positive control (PC) diet was formulated to contain 0.601% Ca and 0.296% standardized total tract digestible (STTD) P, which were marginally lower than NRC (2012) requirement estimates for 25–50 kg pigs. A negative control (NC) diet (0.431% Ca and 0.164% STTD P) was developed by the removal of dicalcium phosphate from the PC diet and replacement with limestone and sand. Diets were: 1) PC, 2) NC, and 3–7) NC + 250, 500, 750, 1,000 and 1,500 FTU phytase/kg diet. On d 36, all pigs were euthanized for femur and metacarpal measures. Compared with NC, pigs fed the PC diet provided greater (P &lt; 0.05) response for ADG (966 vs. 730 g/d), ADFI (1,660 vs. 1,329 g/d); bone measures (37.1 vs. 17.4 g ash/femur and 71.9 vs. 29.3 kg for metacarpal strength), and apparent total tract digestibility (ATTD) of Ca (69.5 vs. 63.5%) and P (62.5 vs. 49.3%). As supplemental phytase increased from 0 to 1,500 FTU/kg of diet, linear and quadratic increases (P &lt; 0.002) occurred in overall ADG and ADFI, femur mineral content, metacarpal breaking strength, and ATTD of Ca and P. Responses statistically similar to PC were achieved at 250 or 500 FTU/kg and peak responses occurred at 750 to 1,500 FTU/kg that were numerically greater than PC for all but metacarpal strength. In conclusion, supplemental OptiPhos Plus improved growth, bone mineralization, Ca and P digestibility of pigs fed Ca and P-deficient diets, demonstrating enhanced Ca and P utilization of corn-soybean meal-based diets by nursery-grower pigs.


2019 ◽  
Vol 19 (1) ◽  
pp. 159-172 ◽  
Author(s):  
Tércia Cesária Reis de Souza ◽  
Araceli Aguilera Barreyro ◽  
Sara Rubio Rubio ◽  
Yanier Machado González ◽  
Konisgmar Escobar García ◽  
...  

AbstractTwo experiments were conducted to test if dehydrated porcine plasma (DPP) and potato protein concentrate (PPC) could be used as an alternative to antibiotics in starter diets for piglets. Experiment one was conducted to test if DPP and PPC in an antibiotic-free diet affected pig performance, and faecal consistency. Eighty-four piglets weaned at 22 days and weighing 6.9 kg were used. Piglets were fed for two weeks with one of four diets: a positive control diet with antibiotics (C+); and three other diets without antibiotics added with DPP, PPC, or DPP and PPC (DPP+PPC) to measure the average daily feed intake (ADFI), average daily gain (ADG), feed efficiency (FE), and incidence and severity of diarrhoea (ID and ISD respectively). In experiment two, twenty-four piglets weaned at 17 days and weighing 5.7 kg, were implanted at 21 days of age with a T-cannula at the terminal ileum to measure the apparent ileal digestibility (AID) and the apparent total tract digestibility (ATTD) of nutrients. Piglets were fed one of four diets: a positive control diet with antibiotics (C+); a negative control diet without antibiotics (C–), and two diets without antibiotics added with DPP, or PPC. The results of experiment one showed that the DPP diet was the most consumed diet during the first week, and the ADG and FE were similar among treatments. During the second week and the total experimental period the ADFI, ADG, and FE were similar among diets. The ID was lower in the C+ diet than other diets. The ISD was lower in the C+ diet than DPP and DPP+PPC diets; piglets fed PPC diet were similar to piglets fed C+ and DPP and DPP+PPC diets. The results of digestibility showed that crude protein AID was higher in piglets fed C+ and PPC diets than C– and DPP diets. Dry matter ATTD and energy ATTD were higher for piglets fed PPC than other diets. Further, crude protein ATTD of DPP and PPC diets tended to have a similar digestibility to that of C+ diet. The results suggest that PPC is a potential controller of post-weaning diarrhoea.


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