Effects of Salt Stress on Germination Percentage and Seedling Growth in Sweet Sorghum Cultivars

2007 ◽  
Vol 7 (8) ◽  
pp. 1492-1495 ◽  
Author(s):  
A. Almodares ◽  
M.R. Hadi . ◽  
B. Dosti .
2018 ◽  
Vol 51 (3) ◽  
pp. 51-68 ◽  
Author(s):  
M.K. Hasan ◽  
M.S. Islam ◽  
M.R. Islam ◽  
H.N. Ismaan ◽  
A. El Sabagh

Abstract A laboratory experiment regarding germination and seedling growth test was conducted with three black gram genotypes tested under three salinity levels (0, 75 and 150 mM), for 10 days, in sand culture within small plastic pot, to investigate the germination and seedling growth characteristics. Different germination traits of all black gram genotypes, like germination percentage (GP), germination rate (GR), coefficient of velocity of germination (CVG) greatly reduced, as well as mean germination time (MGT) increased with increasing salt stress. At high salt stress, BARI Mash-3 provided the highest GP reduction (28.58%), while the lowest was recorded (15.79% to control) in BARI Mash-1. Salinity have the negative impact on shoot and root lengths, fresh and dry weights. The highest (50.32% to control) and lowest reduction (36.39%) of shoot length were recorded in BARI Mash-2 and BARI Mash-1, respectively, under 150 mM NaCl saline conditions. There were significant reduction of root lengths, root fresh and dry weight, shoot length, shoot fresh and dry weight in all genotypes under saline condition. The genotypes were arranged as BARI Mash-1 > BARI Mash-3 > BARI Mash-2, with respect to salinity tolerance.


2021 ◽  
Vol 50 (4) ◽  
pp. 1165-1171
Author(s):  
Kürşat ÇAvuşoğlu ◽  
Di̇lek ÇAvuşoğlu

Effects of L-ornithine (150 mg/l) on the germination, seedling growth, mitotic index, chromosome aberrations and micronucleus frequency of Allium cepa L. bulbs germinated at 0.125 M salinity were studied. The radicle number of the group III bulbs germinated in the medium with ornithine alone as compared to ones of the group I (control) bulbs which germinated in distilled water medium. But, their germination percentage, radicle length and fresh weight were statistically the same as ones of the group I bulbs. Besides, the micronucleus frequency and chromosomal abnormalities in the root-tip meristematic cells of the group III bulbs showed increased germination compared to ones of the group I bulbs. However, their mitotic index statistically showed the same value as the group I bulbs. Salt stress significantly inhibited the germination and seedling growth of A. cepa bulbs. Moreover, it reduced the mitotic index in the root-meristem cells of the bulbs and fairly increased the number of chromosome aberrations and micronucleus frequency. On the other hand, the inhibitive effect of salt on the germination, seedling growth, mitotic index and micronucleus frequency was dramatically alleviated in varying degrees by ornithine application. But, it was ineffective in reducing the detrimental effect of salinity on the chromosome aberrations. The germination percentage, radicle lenght, radicle number, fresh weight, mitotic index, micronucleus frequency and chromosomal aberrations of the group II seedlings grown in 0.125 M salinity were 27%, 13.5 mm, 18.4, 7.1 g, 5.5, 18.3 and 60.8%, respectively while these values became 68%, 16.4 mm, 16.4, 10.5 g, 15.6, 7.6 and 74.8% in the group IV seedlings treated with L-ornithine. Bangladesh J. Bot. 50(4): 1165-1171, 2021 (December)


2017 ◽  
Vol 5 (2) ◽  
pp. 169-176 ◽  
Author(s):  
SUMAN PANTOLA ◽  
KIRAN BARGALI ◽  
S.S. BARGALI ◽  
VIBHUTI VIBHUTI

Among abiotic stresses, drought and salinity are two major determinants due to high magnitude of their impact and wide occurrence. Salinity considerably limits the productivity of crops and thus, considered as the most destructive abiotic factor. In the present study, response of Macrotyloma uniflorum and Vigna mungo to salt (NaCl) stress imposed at germination and seedling growth stages was investigated. The aim of the study was identify the physiological and morphological responses of selected leguminous crop. Seeds were obtained from the healthy plants, surface sterilized and placed under six salt stress levels (0, 4, 8, 12, 16, 20 dsm-1). Complete randomized design with three replicates was used for this experiment and the experiments were conducted during the year 2015 in the glasshouse of Department of Botany, DSB, Campus, Kumaun University, Nainital. In comparison to Macrotyloma uniflorum (77%), higher germination percentage was observed in Vigna mungo (99%) at all salinity levels. In both the species, germination percentage and seedling growth decreased with the increase in salinity stress. Though Vigna mungo showed higher values for root (0.17 g) and shoot dry mass (0.27 g) the dry weight percentage reduction was higher in this species as compared to Macrotyloma uniflorum. Decrease in biomass of seedling with increasing salt stress indicated that the stress not only affected germination but also the growth of seedlings, which indicates that the synthetic ability of seed and biomass of the seedlings was also affected. Seed vigor index declined with the increase in salt concentrations. Outcomes from the study could be helpful in understanding the plant’s nature against different levels of salt stress and that could be economically exploited by various able agencies. At each salinity level, M. uniflorum showed higher salt tolerance index as compared to V. mungo so it can be cultivated in fields with salty soil.


2018 ◽  
Vol 47 (4) ◽  
pp. 831-837
Author(s):  
D. Udhaya Nandhini ◽  
E. Somasundaram

Effects of nod factors (lipo chitooligosaccharide) on seedling growth of maize under salt stress have been studied. The study was framed with seed priming using lipo chitooligosaccharide (LCO) a nod factor @ 4 ml/kg of seeds and without priming on maize seedling growth which was exposed to different levels of salinity (0, 2, 4, 6, 8 and 10 dS/m). Salinity negatively influenced the seedling growth of maize. However nod factor treated maize seedlings had relatively higher germination percentage, root length, shoot length, reducing sugars and amylase activity. Correlation analysis revealed positive relationships between seedling growth parameters. Thus, seed priming with nod factor (4 ml/kg) improved the resistance to salinity at seedling stage. NaCl concentrations restricted amylase enzyme activity and reducing sugar content in the germinating seeds. Priming of nod factor/LCO mitigated the restricted effects of salinity on amylase activity and reducing sugars.


Author(s):  
Shaila Shermin Tania ◽  
Md. Moklasur Rahaman ◽  
Farjana Rauf ◽  
Mehera Afroj Suborna ◽  
Muhammad Humayun Kabir ◽  
...  

Aim: Salinity is a major barrier to successful crop production. Seed priming and exogenous application of different signaling molecules can efficiently confer salinity tolerance. Wheat is a major cereal crop in the world and salinity drastically reduces the wheat seedling growth and yield. Therefore, the present study was conducted to explore the potentiality of different signaling molecules such as salicylic acid (SA) and H2O2 to alleviate the salinity-induced growth inhibition of wheat. Place and Duration of the Study: The study was conducted in the Department of Seed Science and Technology, Bangladesh Agricultural University, from September-October, 2021. Methodology: The wheat (cv. BARI-Gom 24) seeds were soaked in normal tap water (hydro-priming), 1 mM SA, 2 mM SA, 0.1 mM H2O2, and 0.15 mM H2O2 solutions for 30 minutes. The untreated seeds were used as control. Eventually, primed seeds were exposed to 150 mM NaCl in Petri dishes during germination. Primed and non-primed seedlings were grown for 15 days under 150 mM NaCl stress condition. Results: The result revealed that salt stress significantly reduced germination percentage (GP), germination index (GI), seed vigor index (SVI), shoot and root length. The results also exhibited that photosynthetic pigments, total chlorophyll, carotenoids, lycopene, and beta-carotene contents were significantly reduced by salt stress. Seed priming with SA and H2O2 and hydro-priming promoted the germination percentage, seedling growth (including shoot and root length), SVI, and photosynthetic pigments. Conclusion: Pretreatment with 1 mM SA and 0.1 mM H2O2 was observed to be relatively more efficient in conferring salinity tolerance of wheat compared with other treating conditions. Overall, this study suggests that wheat seed priming with SA and H2O2 and hydro-priming can improve salinity tolerance. Aim: Salinity is a major barrier to successful crop production. Seed priming and exogenous application of different signaling molecules can efficiently confer salinity tolerance. Wheat is a major cereal crop in the world and salinity drastically reduces the wheat seedling growth and yield. Therefore, the present study was conducted to explore the potentiality of different signaling molecules such as salicylic acid (SA) and H2O2 to alleviate the salinity-induced growth inhibition of wheat. Place and Duration of the Study: The study was conducted in the Department of Seed Science and Technology, Bangladesh Agricultural University, from September-October, 2021. Methodology: The wheat (cv. BARI-Gom 24) seeds were soaked in normal tap water (hydro-priming), 1 mM SA, 2 mM SA, 0.1 mM H2O2, and 0.15 mM H2O2 solutions for 30 minutes. The untreated seeds were used as control. Eventually, primed seeds were exposed to 150 mM NaCl in Petri dishes during germination. Primed and non-primed seedlings were grown for 15 days under 150 mM NaCl stress condition. Results: The result revealed that salt stress significantly reduced germination percentage (GP), germination index (GI), seed vigor index (SVI), shoot and root length. The results also exhibited that photosynthetic pigments, total chlorophyll, carotenoids, lycopene, and beta-carotene contents were significantly reduced by salt stress. Seed priming with SA and H2O2 and hydro-priming promoted the germination percentage, seedling growth (including shoot and root length), SVI, and photosynthetic pigments. Conclusion: Pretreatment with 1 mM SA and 0.1 mM H2O2 was observed to be relatively more efficient in conferring salinity tolerance of wheat compared with other treating conditions. Overall, this study suggests that wheat seed priming with SA and H2O2 and hydro-priming can improve salinity tolerance.


1970 ◽  
Vol 40 (1) ◽  
pp. 67-73 ◽  
Author(s):  
E Shahbazi ◽  
A Arzani ◽  
G Saeidi

Effects of salt stress on germination, seedling growth and activity of antioxidant enzymes in leaves of six cultivars of canola (Brassica napus L.) were investigated on two F1 hybrids (Hyola401, Hyola330) and four open pollinated cultivars (Zarfam, Okapi, RGs003 and Sarigol). Seeds were germinated under various levels of salinity (0, 50, 100, 150 and 200 mM NaCl solutions). An increase in NaCl concentrations progressively inhibited seed germination. Hyola401 showed the highest germination percentage at all salinity levels. Seedling growth parameters were affected by salt stress particularly at 150 and 200 mM. Leaf antioxidant activities of superoxide dismutase (SOD), ascorbate peroxidase (APX) and glutathione reductase (GR) were increased by salinity increase up to 150 mM while decreased at 200 mM NaCl concentration. Although constitutive levels of activity of antioxidative enzymes were almost the same among the canola cultivars, Hyola401 induced antioxidant enzyme activities were more efficient when subjected to NaCl treatment. Among the tested cultivars, F1 hybrid ‘Hyola401’ could be considered as salt tolerant as possessing higher germination percentage, better seedling growth and antioxidant activities under salinity stress. On the other hand, F1 hybrid ‘Hyola330’ performed inferior to said aspects and was the most susceptible cultivar to salinity stress. Key words: Canola; Brassica napus; Salinity; Antioxidant enzyme; NaCl; Germination DOI: http://dx.doi.org/10.3329/bjb.v40i1.8000 Bangladesh J. Bot. 40(1): 67-73, 2011 (June)


2014 ◽  
Vol 12 ◽  
pp. 5-15 ◽  
Author(s):  
R. Sozharajan ◽  
S. Natarajan

Salt stress is one of the most severe environmental factors that reduces and limits growth and development of plants. Abiotic stresses such as heavy metals, salinity, drought, temperature, UV-radiation, ozone causes drastic yield reduction in most of the crops. Especially salt stress affects around 20 of NaCl on germination and seedling growth of Zea mays L. Seeds of Zea mays were germinated in glass Petri- 100 × 15 mm diameter lined with blotting paper. Ten seeds were placed in each petri-dish. Petridishes were irrigated with 25, 50, 75, 100, 125, 150, 175 and 200 mM concentrations of NaCl. A control was moistened with ten milliliters of distilled water. The germination percentage, water absorption of the seeds, water uptake percentage and the growth parameters were observed. The results obtained showed that the inhibition of the germination percentage, germination rate, water uptake, growth and biomass accumulation of the seedlings were observed to decrease with increasing NaCl concentrations. At the highest level of stress both plumule and radical decreased significantly. The salt stress decreased seed germination, biomass and growth of maize seedlings due to ion toxicity, decrease osmotic potential and oxidative stress


2007 ◽  
Vol 58 (8) ◽  
pp. 811 ◽  
Author(s):  
S. Zhang ◽  
J. Hu ◽  
Y. Zhang ◽  
X. J. Xie ◽  
Allen Knapp

Salt stress is an important constraint to lucerne (Medicago sativa L.) production in many parts of the world. Seeds of 3 lucerne varieties, cvv. Victoria, Golden Empress, and Victor, were used to investigate the effects of seed priming with 5 µm/L brassinolide on germination and seedling growth under a high level of salt stress (13.6 dS/m NaCl solution). The results showed that germination percentage, germination index, and vigour index of lucerne seeds primed with brassinolide were significantly higher than those of the non-primed seeds under salinity stress in each variety. Seed priming with brassinolide significantly increased the shoot fresh weight, shoot dry weight, and root dry weight in 2 varieties, and significantly increased the root length and root vigour in each variety. It also significantly increased the activities of antioxidant enzymes, peroxidase (POD), superoxide dismutase (SOD), and catalase (CAT), in Victoria and Victor seedlings. During seedling growth, the primed seeds significantly reduced the malondialdehyde (MDA) accumulation. This suggests that priming lucerne seed with brassinolide at a suitable concentration can improve germination and seedling growth under high-saline soils.


HortScience ◽  
2020 ◽  
Vol 55 (5) ◽  
pp. 647-650
Author(s):  
Xu-Wen Jiang ◽  
Cheng-Ran Zhang ◽  
Wei-Hua Wang ◽  
Guang-Hai Xu ◽  
Hai-Yan Zhang

The effects of CaCl2, GA3, and H2O2 priming on Isatis indigotica Fort. seed germination characteristics, seedling growth parameters, and antioxidant enzyme activities under salt stress were investigated. NaCl had an adverse effect on the germination and seedling performance of I. indigotica. However, these three priming agents alleviated salt stress by increasing the germination percentage, improving seed vigor, accelerating germination velocity, and establishing strong seedlings. The optimal concentrations were 15 g/L for CaCl2, 0.2 g/L for GA3, and 40 mm for H2O2. Seed priming treatments enhanced the activities of antioxidant enzymes in seedlings, such as superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT), under a salt environment, which reduced the oxidative injury caused by salt. Seed priming is a promising technique that can enhance the ability of I. indigotica seed germination when salt is present.


Author(s):  
Muhammad Abdus Sobahan

Seed priming is a pre-sowing treatment that enhances germination performance and stress tolerance of germinating seeds. The effect of seed priming with proline on germination and seedling growth of mungbean (Vigna radiata L.) under salt stress was investigated. The experiment carried out in completely randomized design with three replications in May 2018 at the Research Laboratory of the School of Agriculture and Rural Development, Bangladesh Open University, Gazipur, Bangladesh. Salt stress at 5 dSm-1 decreased seed germination percentage, plumule length, radicle length, plumule fresh weight, radicle fresh weight and seed vigour index compared to control. Seed priming with proline increased germination percentage (53.84%), plumule length, radicle length, plumule fresh weight, radicle fresh weight and seed vigour index under salt stress. The results suggested that seed priming with proline could effectively alleviate the inhibitory effects of salt stress on seed germination and seedling growth of mungbean. South Asian J. Agric., 7(1&2): 15-18


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