scholarly journals Angiogenesis-Interfering Potential of Wound Healing Plants in Subintestinal Blood Vessels of Tg(fli1a:EGFP)y1/+Zebrafish Embryos

2021 ◽  
Vol 15 (1) ◽  
pp. 7-17
Author(s):  
Dennis R.A. Mans ◽  
Priya Magali ◽  
Awinash Sardjoeper
Keyword(s):  
Wound Healing ◽  
Blood Vessels ◽  
Zebrafish Embryos

scholarly journals Sustainable Development of Chitosan/Calotropis procera-Based Hydrogels to Stimulate Formation of Granulation Tissue and Angiogenesis in Wound Healing Applications

Molecules ◽  
2021 ◽  
Vol 26 (11) ◽  
pp. 3284
Author(s):  
Muhammad Zahid ◽  
Maria Lodhi ◽  
Zulfiqar Ahmad Rehan ◽  
Hamna Tayyab ◽  
Talha Javed ◽  
...  
Keyword(s):  
Wound Healing ◽  
Blood Vessels ◽  
Granulation Tissue ◽  
Chorioallantoic Membrane ◽  
Calotropis Procera ◽  
Connective Tissues ◽  
Blood Capillaries ◽  
Chick Chorioallantoic Membrane ◽  
Thaw Cycle ◽  
Freeze Thaw Cycle

The formation of new scaffolds to enhance healing magnitude is necessarily required in biomedical applications. Granulation tissue formation is a crucial stage of wound healing in which granulation tissue grows on the surface of a wound by the formation of connective tissue and blood vessels. In the present study, porous hydrogels were synthesized using chitosan incorporating latex of the Calotropis procera plant by using a freeze–thaw cycle to stimulate the formation of granulation tissue and angiogenesis in wound healing applications. Structural analysis through Fourier transform infrared (FTIR) spectroscopy confirmed the interaction between chitosan and Calotropis procera. Latex extract containing hydrogel showed slightly higher absorption than the control during water absorption analysis. Thermogravimetric analysis showed high thermal stability of the 60:40 combination of chitosan (CS) and Calotropis procera as compared to all other treatments and controls. A fabricated scaffold application on a chick chorioallantoic membrane (CAM) showed that all hydrogels containing latex extract resulted in a significant formation of blood vessels and regeneration of cells. Overall, the formation of connective tissues and blood capillaries and healing magnitude decreased in ascending order of concentration of extract.

Role of Leukocytes and Endothelial Cells in the Development of Angiogenesis in Inflammation and Wound Healing

2001 ◽  
Vol 125 (1) ◽  
pp. 67-71 ◽  
Author(s):  
Mark W. Lingen
Keyword(s):  
Wound Healing ◽  
Endothelial Cells ◽  
Blood Vessels ◽  
Peripheral Blood ◽  
Inflammatory Process ◽  
Signs And Symptoms ◽  
Critical Component ◽  
Complex Environment ◽  
Seminal Article

Abstract The basic signs and symptoms of inflammation and wound healing have been appreciated for thousands of years. However, the specific cells involved and their roles in this complex environment are still being elucidated today. In 1926, the origin of the phagocytic mononuclear ameboid wandering cell (macrophage) had not been determined. One popular theory was that the cells were differentiated from the endothelial cells of the nearby blood vessels, whereas others believed that the cells came from the peripheral blood or resting wandering cells. The purpose of this article is to review the seminal article published by Lang regarding this topic nearly 75 years ago. In addition, this article will review what is now known with regard to the role of the macrophage and endothelial cells in the development of angiogenesis, which is arguably the most critical component of successful inflammatory process or wound healing.

Opioid Receptors Stimulate Angiogenesis, Lymphangiogenesis and Neurogenesis in Ischemic Wounds in Sickle Mice.

Blood ◽  
2006 ◽  
Vol 108 (11) ◽  
pp. 1199-1199
Author(s):  
Kalpna Gupta ◽  
Yunfang Li ◽  
Nicholas A. Mason ◽  
Tou S. Vang ◽  
Brett K. Levay-Young ◽  
...  
Keyword(s):  
Wound Healing ◽  
Blood Vessels ◽  
Opioid Receptor ◽  
Opioid Receptors ◽  
Culture Medium ◽  
Leg Ulcers ◽  
Cell Disease ◽  
Morphine Treatment ◽  
Post Injury ◽  
Endothelial Proliferation

Abstract Painful leg ulcers are a serious manifestation of sickle cell disease. We showed that topically applied opioids stimulate angiogenesis and promote wound healing in rats, and mice expressing sickle hemoglobin. We hypothesized that opioids orchestrate the normal healing process by stimulating angiogenesis, lymphangiogenesis and neurogenesis. Therefore, we examined mechanism(s) associated with opioid receptor-mediated wound repair using (a) ischemic wounds on transgenic sickle mice with medium severity (hBERK) and control mice expressing normal human hemoglobin (HbABERK), and (b) an in vitro model of skin repair using human epidermal keratinocytes (HEK) and dermal microvascular endothelial cells (HDMEC). We observed HEK secreted β-endorphin into the culture medium ‘immediately’ after injury (9.6±1.3 ng/ml and 2.87±0.37 ng/ml after 30 min of injury), declining to undetectable levels 60 min post-injury. Culture medium from ‘immediately’ injured HEK stimulated about 30% increase in HDMEC proliferation (p < 0.03 vs medium from intact HEK or medium from HEK 24h post-injury), which was completely antagonized by naloxone (1 μM), suggesting an opioid receptor-mediated effect. Furthermore, wounded monolayers of HDMEC incubated with ‘immediately’ injured HEK medium completely healed after 3 d as compared to 4 d with uninjured HEK medium. These data indicate endorphins secreted from skin keratinocytes upon injury stimulate endothelial proliferation, migration and angiogenesis via opioid receptors on endothelium. Therefore, we then examined wound scars from hBERK and HbABERK treated topically with either PBS/cream or morphine (3 mg/g cream) for 13 d. Z-series images were acquired using laser scanning confocal microscopy after immunostaining 100 micron thick wound cryosections for blood vessels, nerves and lymphatics, with anti-CD31, anti-PGP9.5 and anti-lymphatic vessel endothelium marker, respectively. PBS-treated hBERK wounds showed disorganized and stringy blood vessels, nerves and lymphatics, confined to the epidermis vs morphine-treated wounds showing normal architecture and their dermal as well as sub-epidermal localization, similar to that observed in HbA BERK wounds. Stereological quantitation revealed a significantly higher number of blood vessels and nerves in morphine vs PBS treated hBERK wounds (p<0.05). Blood flow estimated by measuring 86Rb uptake by wound scars after tail vein injection showed a 2-fold increase in blood flow in morphine vs PBS treated hBERK wounds (p<0.03), suggesting that opioids stimulate functionally normal vessels in the wounds. Opioid receptors (mu, delta and kappa) co-localized with blood vessels in both HbA and hBERK wounds but, the protein expression of only mu opioid receptor (MOR) was appreciably upregulated by morphine treatment of hBERK wounds as early as 3 d and also after 13 d as observed by Western immunoblotting. In HbABERK morphine-induced MOR upregulation occurred on 3 d but not 13 d. Wounds completely healed 13 d after morphine treatment in HbABERK but not in hBERK. Thus, MOR may be downregulated once the healing has occurred. These data suggest opioids, via their opioid receptors, stimulate endothelial proliferation and normal angiogenesis, lymphangiogenesis and neurogenesis. We speculate that in sickle cell disease where vasculopathy underlies the pathogenesis of painful leg ulcers, topically applied opioids may accelerate wound healing and may even provide pain relief.

scholarly journals Effect and Repair Mechanism of Nano Ag Sponge Dressing Combined with Gelatin-Bletilla Striata Gum/Salvia Miltiorrhiza on Refractory Orthopedic Wounds

2021 ◽  
Vol 2021 ◽  
pp. 1-8
Author(s):  
Chuanjie Jiao ◽  
Ming Deng ◽  
Yonggang Ma ◽  
Geliang Hu
Keyword(s):  
Wound Healing ◽  
Blood Vessels ◽  
Healing Rate ◽  
Salvia Miltiorrhiza ◽  
Collagen Fibers ◽  
Hair Follicles ◽  
Elastic Fibers ◽  
Bletilla Striata ◽  
Ctrl Group ◽  
Tgf Β1

Objective. To explore the effect and mechanism of the sponge dressing on the healing of refractory orthopedic wound, and the gelatin-Bletilla striata gum/Salvia miltiorrhiza nano Ag (GBS-Ag) sponge dressing was prepared. Methods. GBS-Ag sponge dressing was prepared by the freeze-drying method. Twenty male SD rats were randomly divided into the control group (Ctrl group) and GBS-Ag group, with 10 rats in each group, and the rats in the two groups were established a model of back wound infection. The Ctrl group was treated with gauze, while the GBS-Ag group was treated with GBS-Ag sponge dressing. Wound healing rate, blood immune indexes, Ag content in each organ, morphological changes of wound, and expression of transforming growth factor-β1 (TGF-β1) in wound transformation were detected in the two groups of rats. Results. The mechanical properties of GBS-Ag sponge dressing were all in line with the standard, and it had good killing effect on the conventional strain after being incubated for 24 hours. Compared with the Ctrl group, the healing rate and lymphocyte percentage in the GBS-Ag group were significantly increased on day 4 and day 10 ( P < 0.05 ), while the total number of white blood cells and the percentage of neutrophils were significantly decreased ( P < 0.05 ). Compared with Ctrl group, the Ag content in liver, spleen, and kidney of rats in the GBS-Ag group was significantly increased ( P < 0.05 ). The histological results showed that the Ctrl group lacked collagen fibers in the dermis, and the angiogenesis was not rich, accompanied by a large number of inflammatory cell infiltration. The epidermal repair of rats in the GBS-Ag group was complete and partially keratinized, the dermis was rich in collagen fibers, with elastic fibers and new blood vessels, inflammatory cells were rare, and new hair follicles and thick-walled blood vessels were also observed. The expression of TGF-β1 protein in the wounds of rats in the GBS-Ag group was higher than that of the Ctrl group. Conclusion. GBS-Ag sponge dressing had multiple effects of sterilization and promoting wound healing, and its mechanism may be related to promoting the TGF-β1 protein expression.

scholarly journals The Effects of Hydrogen-Rich Water on Skin Wound Healing in Dogs

2021 ◽  
Author(s):  
Dong-Dong Qi ◽  
Ting Wang ◽  
Muhammad Abid Hayat ◽  
Tao Liu ◽  
JianTao Zhang
Keyword(s):  
Wound Healing ◽  
Blood Vessels ◽  
Healing Time ◽  
Skin Wound ◽  
Control Group ◽  
Sod Activity ◽  
Skin Wound Healing ◽  
Histopathological Results ◽  
Wound Healing Time ◽  
Skin Homogenate

Abstract Background: Hydrogen-rich water (HRW) has been shown to be one of underlying therapeutic strategies regarding wound treatments. This study explored the effects of drinking HRW on skin wound healing in dogs. Eight circular wounds were analyzed in each dog. The experimental group was treated with HRW twice daily, while the control group was provided with distilled water (DW). The wound tissues of dogs examined histopathologically. The fibroblasts, inflammatory cell infiltration, the average number of new blood vessels, and the level of malondialdehyde (MDA) and superoxide dismutase (SOD) activity in the skin homogenate of the wound was measured using the corresponding kits. The expression of Nrf-2, HO-1, NQO-1, VEGF, and PDGF were measured by real-time fluorescence quantitative method. Results: We observed that HRW wound had significant average healing rate and faster average healing time. Histopathological results showed that the average number of blood vessels and the average thickness of epidermis were significantly different from the DW group. The MDA levels were higher in the DW group than in the HRW group but the SOD levels were higher in the HRW group than in the DW group. The results of qRT-PCR showed that the expression of each gene was significantly different between the two groups. Conclusions: The results showed that HRW treatment could promote skin wound healing in dogs, accelerate wound epithelization, reduce inflammatory reaction, stimulate the expression of cytokines related to wound healing, and shorten wound healing time.

scholarly journals Targeting Angiogenesis

2014 ◽  
Vol 5 (2) ◽  
pp. 78-86
Author(s):  
Mandeep Kaur
Keyword(s):  
Wound Healing ◽  
Blood Vessels ◽  
Growth And Development ◽  
Angiogenesis Inhibitors ◽  
Capillary Blood ◽  
The Body ◽  
Blood Vessel Formation ◽  
Identification And Characterization ◽  
Head Neck

ABSTRACT Blood vessels constitute the first organ in the embryo and form the largest network in the body, but sadly are often deadly. Angiogenesis is the process of generating new capillary blood vessels. Vasculogenesis is the term used for spontaneous bloodvessel formation, and intussusception is the term for new blood vessel formation by splitting off existing ones. Angiogenesis is a normal and vital process in growth and development, as well as in wound healing and in granulation tissue. It is also a fundamental step in the transition of tumors from a dormant state to a malignant one, leading to the use of angiogenesis inhibitors. Angiogenesis may be a target for combating diseases characterized by either poor vascularization or abnormal vasculature. Application of specific compounds that may inhibit or induce the creation of new blood vessels in the body may help combat such diseases. In this review, we will present an overview of the knowledge gained in studies related to the identification and characterization of different inhibitors and regulators of angiogenesis and also to highlight briefly the pathological and physiological angiogenesis. How to cite this article Kaur M. Targeting Angiogenesis. Int J Head Neck Surg 2014;5(2):78-86.

Laser-induced thermal injury to dermal blood vessels: Analysis of wavelength (585 nm vs. 595 nm), cryogen spray cooling, and wound healing effects

2005 ◽  
Vol 37 (3) ◽  
pp. 210-218 ◽  
Author(s):  
Tianhong Dai ◽  
Parmeswaran Diagaradjane ◽  
Mohammad A. Yaseen ◽  
Brian M. Pikkula ◽  
Sharon Thomsen ◽  
...  
Keyword(s):  
Wound Healing ◽  
Blood Vessels ◽  
Thermal Injury ◽  
Spray Cooling ◽  
Cryogen Spray Cooling

scholarly journals Notch signaling and taxis mechanims regulate early stage angiogenesis: A mathematical and computational model

2019 ◽  
Author(s):  
Rocío Vega ◽  
Manuel Carretero ◽  
Rui D.M. Travasso ◽  
Luis L. Bonilla
Keyword(s):  
Mathematical Model ◽  
Wound Healing ◽  
Numerical Simulations ◽  
Notch Signaling ◽  
Blood Vessels ◽  
Crucial Role ◽  
Early Stage ◽  
Tissue Mechanics ◽  
Organ Development

AbstractDuring angiogenesis, new blood vessels sprout and grow from existing ones. This process plays a crucial role in organ development and repair, in wound healing and in numerous pathological processes such as cancer progression or diabetes. Here, we present a mathematical model of early stage angiogenesis that permits exploration of the relative importance of mechanical, chemical and cellular cues. Endothelial cells proliferate and move over an extracellular matrix by following external gradients of Vessel Endothelial Growth Factor, adhesion and stiffness, which are incorporated to a Cellular Potts model with a finite element description of elasticity. The dynamics of Notch signaling involving Delta-4 and Jagged-1 ligands determines tip cell selection and vessel branching. Through their production rates, competing Jagged-Notch and Delta-Notch dynamics determine the influence of lateral inhibition and lateral induction on the selection of cellular phenotypes, branching of blood vessels, anastomosis (fusion of blood vessels) and angiogenesis velocity. Anastomosis may be favored or impeded depending on the mechanical configuration of strain vectors in the ECM near tip cells. Numerical simulations demonstrate that increasing Jagged production results in pathological vasculatures with thinner and more abundant vessels, which can be compensated by augmenting the production of Delta ligands.Author SummaryAngiogenesis is the process by which new blood vessels grow from existing ones. This process plays a crucial role in organ development, in wound healing and in numerous pathological processes such as cancer growth or in diabetes. Angiogenesis is a complex, multi-step and well regulated process where biochemistry and physics are intertwined. The process entails signaling in vessel cells being driven by both chemical and mechanical mechanisms that result in vascular cell movement, deformation and proliferation. Mathematical models have the ability to bring together these mechanisms in order to explore their relative relevance in vessel growth. Here, we present a mathematical model of early stage angiogenesis that is able to explore the role of biochemical signaling and tissue mechanics. We use this model to unravel the regulating role of Jagged, Notch and Delta dynamics in vascular cells. These membrane proteins have an important part in determining the leading cell in each neo-vascular sprout. Numerical simulations demonstrate that increasing Jagged production results in pathological vasculatures with thinner and more abundant vessels, which can be compensated by augmenting the production of Delta ligands.

Platelet-associated angiogenesis regulating factors: a pharmacological perspective

2012 ◽  
Vol 90 (6) ◽  
pp. 679-688 ◽  
Author(s):  
Aneta Radziwon-Balicka ◽  
Cesar Moncada de la Rosa ◽  
Paul Jurasz
Keyword(s):  
Wound Healing ◽  
Tumor Growth ◽  
Blood Vessels ◽  
Research Group ◽  
Membrane Lipids ◽  
Current Knowledge ◽  
Angiogenesis Inhibitor

Platelets, in addition to maintaining hemostasis, also stimulate angiogenesis by generating and releasing, upon activation, factors that promote the growth of new blood vessels. To date, at least 20 angiogenesis-regulating factors have been identified in platelets, including both promoters and inhibitors. Platelet-derived angiogenesis regulators promote angiogenesis during wound healing, tumor growth, and in response to ischemia. Within platelets, angiogenesis regulators are primarily stored in α-granules, but are also found in the cytosol or derived from membrane lipids. Their release can be inhibited pharmacologically by anti-platelet agents, which consequently suppress platelet-stimulated angiogenesis. Several years ago, our research group discovered that platelets generate the angiogenesis inhibitor angiostatin independent of the activation state of platelets, and that platelet-derived angiostatin serves to limit the angiogenesis-stimulating effects of platelets. In this review, we summarize the current knowledge of platelet-associated angiogenesis regulators, how they impact angiogenesis, and how they are controlled pharmacologically.

Sign in / Sign up

Export Citation Format

Share Document