Advances in Rapid Detection Methods for Foodborne Pathogens

ABSTRACTinvA is a common molecular target for Salmonella-specific detection methods and is recommended by the U.S. Food and Drug Administration Bacteriological Analytical Manual as a target for PCR confirmation of putative Salmonella isolates. Novel assays designed for the rapid detection of foodborne pathogens are often validated according to guidelines provided by validation schemes, such as the AOAC International or the International Organization for Standardization. However, these validation guidelines allow for flexibility in the validation study experimental design, which may inflate the assay's ability to detect foodborne pathogens, especially for foodborne pathogens such as Salmonella, exhibiting tremendous species diversity with >2,600 confirmed serovars. This study was conducted to (i) describe the sequence diversity of invA, across a diverse set of Salmonella serovars and (ii) evaluate the ability of two commercially available, AOAC International–validated rapid detection assays to detect a diverse collection of Salmonella spp. strains. In silico analyses identified 362 of 2,058 nucleotide sites that were variable among invA sequences from a diverse collection, representing 86 unique serovars spanning all species and subspecies. Not surprisingly, the majority of variable sites (308 of 2,058) occurred in non–Salmonella enterica subsp. enterica strains, including Salmonella bongori and the other S. enterica subspecies. In vitro testing showed that both rapid detection assays, examined here, failed to detect all Salmonella strains at 1 log above the limit of detection, with assay A failing to detect S. enterica subsp. salamae, and assay B failing to detect S. bongori. Both strains were eventually detected at 100,000 times the limit of detection. Taken together, our study highlights the need to include non–subsp. S. enterica strains in the development and validation of rapid detection methods to limit false-negative test results.HIGHLIGHTS

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Assessment Criteria and Approaches for Rapid Detection Methods To Be Used in the Food Industry

Journal of Food Protection ◽

10.4315/0362-028x.jfp-13-138 ◽

2014 ◽

Vol 77 (4) ◽

pp. 670-690 ◽

Cited By ~ 11

Author(s):

MARTIN WIEDMANN ◽

SIYUN WANG ◽

LAURIE POST ◽

KENDRA NIGHTINGALE

Keyword(s):

Nucleic Acid ◽

Foodborne Pathogens ◽

Rapid Detection ◽

Food Industry ◽

Pathogen Detection ◽

Assessment Criteria ◽

Detection Methods ◽

Advantages And Disadvantages ◽

Specific Focus

The number of commercially available kits and methods for rapid detection of foodborne pathogens continues to increase at a considerable pace, and the diversity of methods and assay formats is reaching a point where it is very difficult even for experts to weigh the advantages and disadvantages of different methods and to decide which methods to choose for a certain testing need. Although a number of documents outline quantitative criteria that can be used to evaluate different detection methods (e.g., exclusivity and inclusivity), a diversity of criteria is typically used by industry to select specific methods that are used for pathogen detection. This article is intended to provide an overall outline of criteria that the food industry can use to evaluate new rapid detection methods, with a specific focus on nucleic acid–based detection methods.

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Development of an antigen-ELISA and a colloidal gold–based immunochromatographic strip based on monoclonal antibodies for detection of avian influenza A(H5) viruses

Journal of Veterinary Diagnostic Investigation ◽

10.1177/10406387211027538 ◽

2021 ◽

pp. 104063872110275

Author(s):

Yixin Xiao ◽

Fan Yang ◽

Fumin Liu ◽

Linfang Cheng ◽

Hangping Yao ◽

...

Keyword(s):

Avian Influenza ◽

Disease Virus ◽

Colloidal Gold ◽

Rapid Detection ◽

Influenza A ◽

Field Investigation ◽

Cross Reactivity ◽

Detection Methods ◽

Immunochromatographic Strip ◽

Antigen Elisa

Avian influenza A(H5) viruses (avian IAVs) pose a major threat to the economy and public health. We developed an antigen-ELISA (ag-ELISA) and a colloidal gold–based immunochromatographic strip for the rapid detection of avian A(H5) viruses. Both detection methods displayed no cross-reactivity with other viruses (e.g., other avian IAVs, infectious bursal disease virus, Newcastle disease virus, infectious bronchitis virus, avian paramyxovirus). The ag-ELISA was sensitive down to 0.5 hemagglutinin (HA) units/100 µL of avian A(H5) viruses and 7.5 ng/mL of purified H5 HA proteins. The immunochromatographic strip was sensitive down to 1 HA unit/100 µL of avian A(H5) viruses. Both detection methods exhibited good reproducibility with CVs < 10%. For 200 random poultry samples, the sensitivity and specificity of the ag-ELISA were 92.6% and 98.8%, respectively, and for test strips were 88.9% and 98.3%, respectively. Both detection methods displayed high specificity, sensitivity, and stability, making them suitable for rapid detection and field investigation of avian A(H5) viruses.

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Biosensor-based Rapid Detection for Harmful Foodborne Pathogens

2020 7th International Conference on Biomedical and Bioinformatics Engineering ◽

10.1145/3444884.3444906 ◽

2020 ◽

Author(s):

Peitong Xu

Keyword(s):

Foodborne Pathogens ◽

Rapid Detection

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Gold Immunochromatography Assay - A Newly Rapid Detection Technique for Aquatic Products

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.690-693.1449 ◽

2013 ◽

Vol 690-693 ◽

pp. 1449-1454

Author(s):

Yuan Wang ◽

Hui Juan Yu ◽

Bei Lei Qian ◽

You Qiong Cai ◽

Dong Mei Huang ◽

...

Keyword(s):

Rapid Detection ◽

High Sensitivity ◽

Detection Technique ◽

Detection Methods ◽

Future Perspectives ◽

Auxiliary Equipment ◽

Basic Principles ◽

Aquatic Products ◽

Immunological Detection

Gold immunochromatography assay (GICA) technique has the following characteristics: rapid and simple, high sensitivity, good specificity, no auxiliary equipment, ease of interpreting results, and satisfactory stability. The technique has become one of the most rapid and sensitive immunological detection methods, which is widely used in medical, biological and other fields. The article focuses on the basic principles and technical characteristics of GICA, and briefly describes the applications and future perspectives in the rapid detection of aquatic products.

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Understanding the competitive interactions in aptamer–gold nanoparticle based colorimetric assays using surface enhanced Raman spectroscopy (SERS)

Analytical Methods ◽

10.1039/c5ay03158c ◽

2016 ◽

Vol 8 (7) ◽

pp. 1602-1608 ◽

Cited By ~ 12

Author(s):

Shintaro Pang ◽

Lili He

Keyword(s):

Raman Spectroscopy ◽

Gold Nanoparticle ◽

Rapid Detection ◽

Surface Enhanced Raman Spectroscopy ◽

Competitive Interactions ◽

Detection Methods ◽

Color Changes ◽

Surface Enhanced ◽

Surface Enhanced Raman ◽

Colorimetric Assays

Aptamer–gold nanoparticle (AuNP) based colorimetric assays have become increasingly popular as viable rapid detection methods, but the molecular interactions governing the mechanism and successful interpretation of color changes have not been explored well.

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Rapid Detection Methods for Online Monitoring of Vinegar Fermentations

Advances in Vinegar Production ◽

10.1201/9781351208475-20 ◽

2019 ◽

pp. 421-440

Author(s):

Despina Kalogianni

Keyword(s):

Rapid Detection ◽

Online Monitoring ◽

Detection Methods

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Outbreaks, Germination, and Inactivation of Bacillus cereus in Food Products: A Review

Journal of Food Protection ◽

10.4315/0362-028x.jfp-19-429 ◽

2020 ◽

Vol 83 (9) ◽

pp. 1480-1487

Author(s):

WON CHOI ◽

SANG-SOON KIM

Keyword(s):

Food Safety ◽

Bacillus Cereus ◽

Food Processing ◽

Rapid Detection ◽

Foodborne Pathogen ◽

Present Review ◽

Detection Methods ◽

Processing Technologies ◽

Foodborne Outbreaks ◽

Food Commodity

ABSTRACT Bacillus cereus has been reported as a foodborne pathogen worldwide. Although food processing technologies to inactivate the pathogen have been developed for decades, foodborne outbreaks related to B. cereus have occurred. In the present review, foodborne outbreaks, germination, inactivation, and detection of B. cereus are discussed, along with inactivation mechanisms. B. cereus outbreaks from 2003 to 2016 are reported based on food commodity, number of cases, and consequent illnesses. Germination before sporicidal treatments is highlighted as an effective way to inactivate B. cereus, because the resistance of the pathogen increases significantly following sporulation. Several germinants used for B. cereus are listed, and their efficacies are compared. Finally, recently used interventions with sporicidal mechanisms are identified, and rapid detection methods that have been developed are discussed. Combining two or more interventions, known as the hurdle technology concept, is suggested to maximize the sporicidal effect. Further study is needed to ensure food safety and to understand germination mechanisms and sporicidal resistance of B. cereus. HIGHLIGHTS

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A Case Series of Parechovirus Encephalopathy: Apnea and Autonomic Dysregulation in Critically Ill Infants

Journal of Child Neurology ◽

10.1177/0883073818789317 ◽

2018 ◽

Vol 33 (12) ◽

pp. 788-793

Author(s):

Elizabeth H. Ristagno ◽

Sonam C. Bhalla ◽

Lindsey K. Rasmussen

Keyword(s):

Intracranial Hypertension ◽

Critically Ill ◽

Rapid Detection ◽

Early Recognition ◽

Case Series ◽

Clinical Entity ◽

Detection Methods ◽

Autonomic Dysregulation

This article aims to describe a rare cause of severe encephalitis in 2 cases of infants with signs of intracranial hypertension and severe autonomic dysregulation. The authors conclude that human parechoviruses are becoming a more recognized cause of encephalitis because of the increasing use of rapid detection methods. With early recognition of this clinical entity, improved care can be administered.

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