FERRITIN-RED FLUORESCENT PROTEIN FUSION REPORTER FOR MAGNETIC RESONANCE AND OPTICAL IMAGING

2012 ◽  
Vol 24 (04) ◽  
pp. 333-341 ◽  
Author(s):  
Shan-Wen Liu ◽  
Kuan-Hung Cho ◽  
Mei-Ru Chen ◽  
Hsiao-Chi Yu ◽  
Yu-Ying Kao ◽  
...  
Keyword(s):  
Magnetic Resonance ◽  
Optical Imaging ◽  
Fluorescent Protein ◽  
Red Fluorescent Protein ◽  
Protein Fusion ◽  
Red Fluorescence ◽  
Infected Cells ◽  
Magnetic Resonance Imaging Mri ◽  
High Level ◽  
In Cells

In this report, we generated a ferritin and red fluorescent protein fusion reporter gene that enables the visualization of transgene expression in living animals by magnetic resonance imaging (MRI) and optical imaging. Ferritin heavy chain (FTH) or light chain (FTL) was linked to the N terminus of the monomeric DsRed red fluorescent protein to create FTH-DsRed and FTL-DsRed, MRI-fluorescence dual reporters. Transfection of these dual reporters into cells resulted in increased iron loading and strong red fluorescence in cells. Adenoviral vectors to express FTH-DsRed or FTL-DsRed fusion reporter in infected cells were created, but only the adenovirus expressing FTH-DsRed resulted in a high level of red fluorescence in cells. Delivery and expression of FTH-DsRed in the mouse brain using adenovirus was detected by MRI and fluorescence imaging, revealing a T2shortening effect and an increase of contrast in T2-weighted images at the sites co-localized with strong red fluorescence. While the details of the structure of ferritin-DsRed fusion reporter remains to be solved, this dual reporter is useful for visualizing dynamic processes such as the migration of reporter-transfected stem cells or metastasis of tumors using MRI with the added flexibility of combining optical tools such as fluorescence activated cell sorting and fluorescence microscopy.

Top-Ten Tips for Imaging the Brachial Plexus with Ultrasound and MRI

2019 ◽  
Vol 23 (04) ◽  
pp. 405-418 ◽  
Author(s):  
James F. Griffith ◽  
Radhesh Krishna Lalam
Keyword(s):  
Magnetic Resonance Imaging ◽  
Magnetic Resonance ◽  
Brachial Plexus ◽  
Muscle Denervation ◽  
Resonance Imaging ◽  
Neural Injury ◽  
Clinical Indications ◽  
Magnetic Resonance Imaging Mri ◽  
High Level ◽  
Extensive Involvement

AbstractWhen it comes to examining the brachial plexus, ultrasound (US) and magnetic resonance imaging (MRI) are complementary investigations. US is well placed for screening most extraforaminal pathologies, whereas MRI is more sensitive and accurate for specific clinical indications. For example, MRI is probably the preferred technique for assessment of trauma because it enables a thorough evaluation of both the intraspinal and extraspinal elements, although US can depict extraforaminal neural injury with a high level of accuracy. Conversely, US is probably the preferred technique for examination of neurologic amyotrophy because a more extensive involvement beyond the brachial plexus is the norm, although MRI is more sensitive than US for evaluating muscle denervation associated with this entity. With this synergy in mind, this review highlights the tips for examining the brachial plexus with US and MRI.

scholarly journals Design and Synthesis of Luminescent Lanthanide-Based Bimodal Nanoprobes for Dual Magnetic Resonance (MR) and Optical Imaging

Nanomaterials ◽  
2021 ◽  
Vol 11 (2) ◽  
pp. 354
Author(s):  
Walid Mnasri ◽  
Mahsa Parvizian ◽  
Souad Ammar-Merah
Keyword(s):  
Magnetic Resonance ◽  
Optical Imaging ◽  
Imaging Technique ◽  
Imaging Techniques ◽  
Design And Synthesis ◽  
Bimodal Imaging ◽  
Successive Image ◽  
Complete Set ◽  
Magnetic Resonance Imaging Mri ◽  
Physical And Chemical

Current biomedical imaging techniques are crucial for the diagnosis of various diseases. Each imaging technique uses specific probes that, although each one has its own merits, do not encompass all the functionalities required for comprehensive imaging (sensitivity, non-invasiveness, etc.). Bimodal imaging methods are therefore rapidly becoming an important topic in advanced healthcare. This bimodality can be achieved by successive image acquisitions involving different and independent probes, one for each mode, with the risk of artifacts. It can be also achieved simultaneously by using a single probe combining a complete set of physical and chemical characteristics, in order to record complementary views of the same biological object at the same time. In this scenario, and focusing on bimodal magnetic resonance imaging (MRI) and optical imaging (OI), probes can be engineered by the attachment, more or less covalently, of a contrast agent (CA) to an organic or inorganic dye, or by designing single objects containing both the optical emitter and MRI-active dipole. If in the first type of system, there is frequent concern that at some point the dye may dissociate from the magnetic dipole, it may not in the second type. This review aims to present a summary of current activity relating to this kind of dual probes, with a special emphasis on lanthanide-based luminescent nano-objects.

scholarly journals Tula hantavirus L protein is a 250 kDa perinuclear membrane-associated protein

2004 ◽  
Vol 85 (5) ◽  
pp. 1181-1189 ◽  
Author(s):  
Sami K. J. Kukkonen ◽  
Antti Vaheri ◽  
Alexander Plyusnin
Keyword(s):  
Matrix Protein ◽  
Fluorescent Protein ◽  
Protein Localization ◽  
Expression System ◽  
Peak Level ◽  
Cell Fractionation ◽  
L Protein ◽  
System L ◽  
Infected Cells ◽  
In Cells

The complete open reading frame of Tula hantavirus (TULV) L RNA was cloned in three parts. The middle third (nt 2191–4344) could be expressed in E. coli and was used to immunize rabbits. The resultant antiserum was then used to immunoblot concentrated TULV and infected Vero E6 cells. The L protein of a hantavirus was detected, for the first time, in infected cells and was found to be expressed as a single protein with an apparent molecular mass of 250 kDa in both virions and infected cells. Using the antiserum, the expression level of the L protein was followed and image analysis of immunoblots indicated that there were 104 copies per cell at the peak level of expression. The antiserum was also used to detect the L protein in cell fractionation studies. In cells infected with TULV and cells expressing recombinant L, the protein pelleted with the microsomal membrane fraction. The membrane association was confirmed with membrane flotation assays. To visualize L protein localization in cells, a fusion protein of L and enhanced green fluorescent protein, L–EGFP, was expressed in Vero E6 cells with a plasmid-driven T7 expression system. L–EGFP localized in the perinuclear region where it had partial co-localization with the Golgi matrix protein GM130 and the TULV nucleocapsid protein.

scholarly journals Fluorinated PLGA-PEG-Mannose Nanoparticles for Tumor-Associated Macrophage Detection by Optical Imaging and MRI

2021 ◽  
Vol 8 ◽  
Author(s):  
Giorgia Zambito ◽  
Siyuan Deng ◽  
Joost Haeck ◽  
Natasa Gaspar ◽  
Uwe Himmelreich ◽  
...  
Keyword(s):  
Magnetic Resonance ◽  
Optical Imaging ◽  
Tumor Development ◽  
Mannose Receptor ◽  
The Body ◽  
Mri Contrast Agent ◽  
Therapeutic Interventions ◽  
Magnetic Resonance Imaging Mri

Tumor-associated macrophages (TAMs) promote cancer growth and metastasis, but their role in tumor development needs to be fully understood due to the dynamic changes of tumor microenvironment (TME). Here, we report an approach to visualize TAMs by optical imaging and by Fluorine-19 (19F) magnetic resonance imaging (MRI) that is largely applied to track immune cells in vivo. TAMs are targeted with PLGA-PEG-mannose nanoparticles (NPs) encapsulating perfluoro-15-crown-5-ether (PFCE) as MRI contrast agent. These particles are preferentially recognized and phagocytized by TAMs that overexpress the mannose receptor (MRC1/CD206). The PLGA-PEG-mannose NPs are not toxic and they were up-taken by macrophages as confirmed by in vitro confocal microscopy. At 48 h after intravenous injection of PLGA-PEG-mannose NPs, 4T1 xenograft mice were imaged and fluorine-19 nuclear magnetic resonance confirmed nanoparticle retention at the tumor site. Because of the lack of 19F background in the body, observed 19F signals are robust and exhibit an excellent degree of specificity. In vivo imaging of TAMs in the TME by 19F MRI opens the possibility for detection of cancer at earlier stage and for prompt therapeutic interventions in solid tumors.

scholarly journals Leader of the Capsid Protein in Feline Calicivirus Promotes Replication of Norwalk Virus in Cell Culture

2008 ◽  
Vol 82 (19) ◽  
pp. 9306-9317 ◽  
Author(s):  
Kyeong-Ok Chang ◽  
David W. George ◽  
John B. Patton ◽  
Kim Y. Green ◽  
Stanislav V. Sosnovtsev
Keyword(s):  
Cell Culture ◽  
Capsid Protein ◽  
Recombinant Plasmid ◽  
Fluorescent Protein ◽  
Feline Calicivirus ◽  
Norwalk Virus ◽  
Human Noroviruses ◽  
Infected Cells ◽  
Green Fluorescent ◽  
In Cells

ABSTRACT The inability to grow human noroviruses in cell culture has greatly impeded the studies of their pathogenesis and immunity. Vesiviruses, in the family Caliciviridae, grow efficiently in cell culture and encode a unique protein in the subgenomic region designated as leader of the capsid protein (LC). We hypothesized that LC might be associated with the efficient replication of vesiviruses in cell culture and promote the replication of human norovirus in cells. To test this hypothesis, a recombinant plasmid was engineered in which the LC region of feline calicivirus (FCV) was placed under the control of the cytomegalovirus promoter (pCI-LC) so that the LC protein could be provided in trans to replicating calicivirus genomes bearing a reporter gene. We constructed pNV-GFP, a recombinant plasmid containing a full-length NV genome with a green fluorescent protein (GFP) in the place of VP1. The transfection of pNV-GFP in MVA-T7-infected cells produced few GFP-positive cells detected by fluorescence microscopy and flow cytometry analysis. When pNV-GFP was cotransfected with pCI-LC in MVA-T7-infected cells, we observed an increase in the number of GFP-positive cells (ca. 3% of the whole-cell population). Using this cotransfection method with mutagenesis study, we identified potential cis-acting elements at the start of subgenomic RNA and the 3′ end of NV genome for the virus replication. We conclude that LC may be a viral factor which promotes the replication of NV in cells, which could provide a clue to growing the fastidious human noroviruses in cell culture.

scholarly journals High Correlation of Whole-Body Red Fluorescent Protein Imaging and Magnetic Resonance Imaging on an Orthotopic Model of Pancreatic Cancer

2005 ◽  
Vol 65 (21) ◽  
pp. 9829-9833 ◽  
Author(s):  
Michael Bouvet ◽  
Joseph Spernyak ◽  
Matthew H. Katz ◽  
Richard V. Mazurchuk ◽  
Shinako Takimoto ◽  
...  
Keyword(s):  
Magnetic Resonance Imaging ◽  
Pancreatic Cancer ◽  
Magnetic Resonance ◽  
Fluorescent Protein ◽  
Whole Body ◽  
Red Fluorescent Protein ◽  
Resonance Imaging ◽  
Orthotopic Model ◽  
Protein Imaging

PEG–MWCNT/Fe hybrids as multi-modal contrast agents for MRI and optical imaging

RSC Advances ◽  
2016 ◽  
Vol 6 (55) ◽  
pp. 49891-49902 ◽  
Author(s):  
Anna Baranowska-Korczyc ◽  
Małgorzata Jasiurkowska-Delaporte ◽  
Barbara M. Maciejewska ◽  
Alicja Warowicka ◽  
L. Emerson Coy ◽  
...  
Keyword(s):  
Magnetic Resonance Imaging ◽  
Polyethylene Glycol ◽  
Carbon Nanotube ◽  
Magnetic Resonance ◽  
Fluorescence Microscopy ◽  
Contrast Agents ◽  
Optical Imaging ◽  
Resonance Imaging ◽  
Multi Walled Carbon Nanotube ◽  
Magnetic Resonance Imaging Mri

This study examines the use of oxidized multi-walled carbon nanotube/iron (O-MWCNT/Fe) nanohybrids modified with polyethylene glycol (PEG) as multifunctional cellular imaging agents for magnetic resonance imaging (MRI) and fluorescence microscopy.

scholarly journals Highly Visible Expression of an Oxytocin-Monomeric Red Fluorescent Protein 1 Fusion Gene in the Hypothalamus and Posterior Pituitary of Transgenic Rats

Endocrinology ◽  
2011 ◽  
Vol 152 (7) ◽  
pp. 2768-2774 ◽  
Author(s):  
Akiko Katoh ◽  
Hiroaki Fujihara ◽  
Toyoaki Ohbuchi ◽  
Tatsushi Onaka ◽  
Takashi Hashimoto ◽  
...  
Keyword(s):  
Median Eminence ◽  
Fluorescent Protein ◽  
Fusion Gene ◽  
Female Rats ◽  
Posterior Pituitary ◽  
Red Fluorescent Protein ◽  
Protein Fusion ◽  
Transgenic Rats ◽  
Salt Loading ◽  
Male And Female

We have generated rats bearing an oxytocin (OXT)-monomeric red fluorescent protein 1 (mRFP1) fusion transgene. The mRFP1 fluorescence was highly visible in ventral part of the supraoptic nucleus (SON) and the posterior pituitary in a whole mount. mRFP1 fluorescence in hypothalamic sections was also observed in the SON, the paraventricular nucleus (PVN), and the internal layer of the median eminence. Salt loading for 5 d caused a marked increase in mRFP1 fluorescence in the SON, the PVN, the median eminence, and the posterior pituitary. In situ hybridization histochemistry revealed that the expression of the mRNA encoding the OXT-mRFP1 fusion gene was observed in the SON and the PVN of euhydrated rats and increased dramatically after chronic salt loading. The expression of the endogenous OXT and the arginine vasopressin (AVP) genes were significantly increased in the SON and the PVN after chronic salt loading in both nontransgenic and transgenic rats. These responses were not different between male and female rats. Compared with nontransgenic rats, euhydrated and salt-loaded male and female transgenic rats showed no significant differences in plasma osmolality, sodium concentration, OXT, and AVP levels. Finally, we succeeded in generating a double-transgenic rat that expresses both the OXT-mRFP1 fusion gene and the AVP-enhanced green fluorescent protein fusion gene. Our new transgenic rats are valuable new tools to study the physiology of the hypothalamo-neurohypophysial system.

Photoluminescence and magnetism integrated multifunctional black phosphorus probes through controllable P=O bonds orbital hybridization

2021 ◽  
Author(s):  
Shuyi Wu ◽  
R. L. Qi ◽  
Chunlan Ma ◽  
Yun Shan ◽  
Y.J. Wu ◽  
...  
Keyword(s):  
Magnetic Resonance Imaging ◽  
Magnetic Resonance ◽  
Optical Imaging ◽  
Clinical Diagnosis ◽  
Critical Role ◽  
Black Phosphorus ◽  
Resonance Imaging ◽  
Fluorescence Optical Imaging ◽  
Orbital Hybridization ◽  
Magnetic Resonance Imaging Mri

Biological probes with integrating photoluminescence and magnetism characteristic play a critical role in modern clinical diagnosis and surgical protocols combining fluorescence optical imaging (FOI) with magnetic resonance imaging (MRI) technology....

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