scholarly journals Loss and re-establishment of desiccation tolerance in the germinated seeds of Sesbania virgata (Cav.) (Pers.)

2015 ◽  
Vol 37 (3) ◽  
pp. 313 ◽  
Author(s):  
Tathiana Elisa Masetto ◽  
José Marcio Rocha Faria ◽  
Ana Carla Rezende Fraiz
Keyword(s):  
Desiccation Tolerance ◽  
Sesbania Virgata ◽  
Germinated Seeds

scholarly journals Cell changes during the re-induction of desiccation tolerance in germinated seeds of Sesbania virgata (Cav.) Pers.

2016 ◽  
Vol 38 (3) ◽  
pp. 254-258 ◽  
Author(s):  
Tathiana Elisa Masetto ◽  
Ana Carla Resende Fraiz ◽  
José Márcio Rocha Faria
Keyword(s):  
Desiccation Tolerance ◽  
Dna Degradation ◽  
Recalcitrant Seeds ◽  
Cell Nuclei ◽  
Green Fluorescence ◽  
Transmission Electron ◽  
Sesbania Virgata ◽  
Orthodox Seeds ◽  
Cell Changes ◽  
Germinated Seeds

Abstract During germination, orthodox seeds lose their ability to tolerate desiccation resembling recalcitrant seeds. This research aimed to investigate the cell changes during the re-induction of the desiccation tolerance (DT) in Sesbania virgata germinated seeds with 1, 3 and 5 mm long radicles. To re-establish DT, germinated seeds were incubated for 72 h in polyethylene glycol (PEG, -2.04 MPa) before dehydration in silica gel (at 10% moisture content) followed by rehydration. Cell viability was assessed through TUNEL test in dry radicles and transmission electron microscopy in both fresh and dry radicles. The positive-TUNEL confirmed the DNA degradation, through the green fluorescence of the cell nuclei from 5 mm radicle length and the ultra structural evaluations detected loss of cellular content integrity in 3 and 5 mm cell radicles that did not survive dehydration to 10%.

scholarly journals In situ DNA fragmentation during the re-establishment of desiccation tolerance in germinated seeds of Cedrela fissilis Vell.

2019 ◽  
Vol 41 (2) ◽  
pp. 244-249
Author(s):  
Tathiana Elisa Masetto ◽  
José Marcio Rocha Faria
Keyword(s):  
Cell Death ◽  
Moisture Content ◽  
Dna Fragmentation ◽  
Desiccation Tolerance ◽  
Cell Injury ◽  
Tunel Staining ◽  
Long Term Storage ◽  
Germinated Seeds

Abstract: Dehydration is a necessary procedure prior to exposing seeds to long term storage, but this is associated with metabolism-linked injury mediated by cell injury. In order to assess cellular alterations during re-establishment of desiccation tolerance (DT) in C. fissilis germinated seeds and their relation to DNA damage, we verified the occurrence of DNA fragmentation through the TUNEL test and its evidence through the cytological analyses. To re-establish DT, germinated seeds were incubated for 72 h in polyethylene glycol (PEG, -2.04 MPa) before dehydration in silica gel (at 10% moisture content) followed by rehydration. The moisture content changes during the reestablishment of the desiccation tolerance was accomplished. (DT)TdT-dUPT terminal nick-end labeling (TUNEL) was used to assess rates of cell death. TUNEL staining was performed using Click-iT-TUNEL Alexa Flour imaging assay. The TUNEL test showed a consistent DNA fragmentation in the 2 and 5 mm long radicles. Moreover, nuclear and chromosomal alterations were observed in the 5 mm meristematic root cell cycle, contributing to the identification of diagnostic markers of cell death.

Induction of desiccation tolerance in germinated seeds

1995 ◽  
Vol 5 (1) ◽  
pp. 1-4 ◽  
Author(s):  
Tonko Bruggink ◽  
Peter van der Toorn
Keyword(s):  
Desiccation Tolerance ◽  
Model System ◽  
Soluble Proteins ◽  
Sucrose Content ◽  
Agricultural Industry ◽  
Convenient Model ◽  
Germinated Seeds

AbstractThe induction of desiccation tolerance in germinated seeds by incubation in PEG for several days is reported. The induction coincided with an increased sucrose content and the formation of heat-soluble proteins. Germinated seeds can serve as a convenient model system in studies of desiccation tolerance. This finding may have important implications for the agricultural industry.

Induction of longevity in primed seeds

1999 ◽  
Vol 9 (1) ◽  
pp. 49-53 ◽  
Author(s):  
G.T. Bruggink ◽  
J.J.J. Ooms ◽  
P. van der Toorn
Keyword(s):  
Water Stress ◽  
Temperature Stress ◽  
Desiccation Tolerance ◽  
Side Effect ◽  
Temperature Dependent ◽  
Negative Side ◽  
Negative Side Effect ◽  
Optimal Duration ◽  
Reduced Time ◽  
Germinated Seeds

AbstractPriming of seeds is generally intended to reduce time to germination, often leading to improved emergence. However, as a negative side effect, priming reduces longevity of seeds. We studied the possibilities to obtain primed seeds with reduced time to germination but with longevity similar to that of untreated seeds. For several species tested we found that the desired longevity could be obtained by keeping the seeds, after a priming treatment, under a mild water and / or temperature stress for a period of several hours to days. Time to germination did not increase again due to such a treatment. Optimal duration and degree of water stress were strongly temperature dependent. The methods applied to obtain primed seeds without loss of longevity are very similar to those used to induce desiccation tolerance in germinated seeds.

The re-establishment of desiccation tolerance in germinated radicles of Medicago truncatula Gaertn. seeds

2003 ◽  
Vol 13 (4) ◽  
pp. 273-286 ◽  
Author(s):  
Julia Buitink ◽  
Benoit Ly Vu ◽  
Pascale Satour ◽  
Olivier Leprince
Keyword(s):  
Gene Expression ◽  
Polyethylene Glycol ◽  
Medicago Truncatula ◽  
Desiccation Tolerance ◽  
Cold Treatment ◽  
Induced Expression ◽  
Peg Treatment ◽  
Osmotic Treatment ◽  
Dehydrin Gene ◽  
Germinated Seeds

Germinated seeds of Medicago truncatula Gaertn. with a protruded radicle length of 2.7 mm did not survive drying below 0.2 g H2O g–1 dw, as indicated by vital stain assays and the absence of growth resumption after rehydration. The re-establishment of desiccation tolerance was achieved using an osmotic treatment with polyethylene glycol (PEG), combined with a cold treatment. The ability to regain desiccation tolerance after germination was restricted to a period of growth characterized by radicle lengths between 1 and 3 mm. After PEG treatment of germinated seeds with 2.7 mm long radicles at –1.7 MPa at 10°C for 3 d and subsequent drying to 0.04 g H2O g–1 dw, 90% survived and developed into normal seedlings after rehydration. Desiccation tolerance could also be re-established in excised radicles, demonstrating that cotyledons were not essential for this process. Upon PEG incubation, sucrose accumulated rapidly prior to the re-establishment of desiccation tolerance in germinated radicles, regardless of the presence of cotyledons. Induction of MtDHN (a dehydrin) gene expression was correlated with the re-establishment of desiccation tolerance. Furthermore, the PEG-induced expression of MtDHN was repressed when fluridone was added to the PEG solution.

scholarly journals Physiological, cellular and molecular aspects of the desiccation tolerance in Anadenanthera colubrina seeds during germination

2017 ◽  
Vol 77 (4) ◽  
pp. 774-780 ◽  
Author(s):  
L. E. Castro ◽  
C. C. Guimarães ◽  
J. M. R. Faria
Keyword(s):  
Dna Content ◽  
Desiccation Tolerance ◽  
Recalcitrant Seeds ◽  
Heat Stable ◽  
Germination Process ◽  
Orthodox Seeds ◽  
Ultrastructural Characteristics ◽  
Molecular Aspects ◽  
Germinated Seeds ◽  
4C Dna Content

Abstract During germination, orthodox seeds become gradually intolerant to desiccation, and for this reason, they are a good model for recalcitrance studies. In the present work, physiological, biochemical, and ultrastructural aspects of the desiccation tolerance were characterized during the germination process of Anadenanthera colubrina seeds. The seeds were imbibed during zero (control), 2, 8, 12 (no germinated seeds), and 18 hours (germinated seeds with 1 mm protruded radicle); then they were dried for 72 hours, rehydrated and evaluated for survivorship. Along the imbibition, cytometric and ultrastructural analysis were performed, besides the extraction of the heat-stable proteins. Posteriorly to imbibition and drying, the evaluation of ultrastructural damages was performed. Desiccation tolerance was fully lost after root protrusion. There was no increase in 4C DNA content after the loss of desiccation tolerance. Ultrastructural characteristics of cells from 1mm roots resembled those found in the recalcitrant seeds, in both hydrated and dehydrated states. The loss of desiccation tolerance coincided with the reduction of heat-stable proteins.

scholarly journals Desiccation tolerance and longevity of germinated Sesbania virgata (Cav.) Pers.seeds

2016 ◽  
Vol 38 (1) ◽  
pp. 50-56 ◽  
Author(s):  
Maria Cecília Dias Costa ◽  
José Marcio Rocha Faria ◽  
Anderson Cleiton José ◽  
Wilco Ligterink ◽  
Henk W.M. Hilhorst
Keyword(s):  
Desiccation Tolerance ◽  
Molecular Mechanisms ◽  
Soil Seed Bank ◽  
Lateral Roots ◽  
Seed Longevity ◽  
Artificial Ageing ◽  
Seed Desiccation ◽  
Sensitive Organ ◽  
Sesbania Virgata ◽  
Osmotic Treatment

Abstract: Seed desiccation tolerance (DT) and longevity are necessary for better dissemination of plant species and establishment of soil seed bank. They are acquired by orthodox seeds during the maturation phase of development and lost upon germination. DT can be re-induced in germinated seeds by an osmotic and/or abscisic acid treatment. However, there is no information on how these treatments affect seed longevity. Germinated Sesbania virgata seeds were used as a model system to investigate the effects of an osmotic treatment to re-establish DT on seed longevity. Longevity of germinated S. virgata seeds treated and non-treated by an osmoticum was analysed after storage or artificial ageing. The radicle is the most sensitive organ, the cotyledons are the most resistant, and the ability to produce lateral roots is the key for whole seed survival. Germinated S. virgata seeds with 1mm protruded radicle tolerate desiccation and storage for up to three months without significant losses in viability. An osmotic treatment can improve DT in these seeds, but not longevity. Germinated S. virgata seeds are a good model to study DT uncoupled from longevity. Further studies are necessary to unveil the molecular mechanisms involved in both DT and longevity.

scholarly journals Seedling survival of Handroanthus impetiginosus (Mart ex DC) Mattos in a semi-arid environment through modified germination speed and post-germination desiccation tolerance

2015 ◽  
Vol 75 (4) ◽  
pp. 812-820 ◽  
Author(s):  
J. R. Martins ◽  
A. A. S. Edvaldo ◽  
A. A. Alvarenga ◽  
A. C. Rodrigues ◽  
D. E. Ribeiro ◽  
...  
Keyword(s):  
Stress Tolerance ◽  
Desiccation Tolerance ◽  
Rainy Season ◽  
Additional Stress ◽  
Tolerance Mechanism ◽  
Wide Range ◽  
Stress Avoidance ◽  
Semi Arid ◽  
Handroanthus Impetiginosus ◽  
Germinated Seeds

Abstract Uniform rapid seed germination generally forms a great risk for the plant population if subsequent intermittent precipitation causes desiccation and seedling death. Handroanthus impetiginosus can be found commonly in a wide range of biomes within Brazil including those that are semi-arid. Germination and early growth was studied to understand how germinated seeds survive under these stringent conditions. Accessions were sampled from four seasonally dry biomes in Brazil. Precipitation at the start of the rainy season in the Caatinga, a semi-arid biome, is less predictable and the number of successive dry days per dry interval in the first four months of the rainy season was higher than in the other studied biomes. Plants from the Caatinga produced thicker seeds and this trait concurred with slow germination and stronger osmotic inhibition of germination across the accessions, forming a stress avoidance mechanism in the Caatinga. Post-germination desiccation tolerance was high in the Caatinga accession, could be re-induced in accessions from biomes with more regular precipitation (Cerrado and transition zone), but remained poor in the Cerradão accession; thus forming a stress tolerance mechanism. Production of adventitious roots ascertained survival of all tested individuals from all four locations, even if protruded radicles did not survive desiccation, forming an additional stress tolerance mechanism. A sequence of stress avoidance and stress tolerance mechanisms in seeds and germinated seeds was associated with precipitation patterns in different biomes. These mechanisms purportedly allow rapid seedling establishment when conditions are suitable and enable survival of the young seedling when conditions are adverse.

scholarly journals Re-induction of desiccation tolerance after germination of Cedrela fissilis Vell. seeds

2014 ◽  
Vol 86 (3) ◽  
pp. 1273-1286 ◽  
Author(s):  
TATHIANA E. MASETTO ◽  
JOSE M. FARIA ◽  
ANA C.R. FRAIZ
Keyword(s):  
Polyethylene Glycol ◽  
Moisture Content ◽  
Cell Viability ◽  
Desiccation Tolerance ◽  
Dna Integrity ◽  
Electronic Microscopy ◽  
Percentage Points ◽  
Cell Changes ◽  
Peg 8000 ◽  
Germinated Seeds

This work aimed to characterize the re-induction of desiccation tolerance (DT) in germinated seeds, using polyethylene glycol (PEG 8000). Cell changes were investigated through cytological assays (cell viability and transmission electronic microscopy) as well as DNA integrity during loss and re-establishment of DT. The loss of DT was characterized by drying germinated seeds with different radicle lengths (1, 2, 3, 4 and 5 mm) in silica gel, decreasing the moisture content to ten percentage points intervals, followed by pre-humidification (100% RH / 24 h) and rehydration. To re-induce DT, germinated seeds were treated for 72 h with PEG (-2.04 MPa) and PEG (-2.04 MPa) + ABA (100 µM) before dehydration. Germinated seeds did not tolerate desiccation to 10% moisture content, irrespectively of the radicle length. However, when incubated in PEG, those with 1 and 2 mm long radicle attained 71% and 29% survival, respectively. The PEG+ABA treatment was efficient to re-establish DT in seeds with 1 mm long radicles (100% survival). The ultrastructural assays of the cells of germinated seeds with 2 and 5 mm length confirmed the obtained physiological results. Germinated seeds of C. fissilis constitute a useful tool for desiccation tolerance investigations.

scholarly journals Maturation and Desiccation Tolerance in Seeds of Sesbania virgata (Cav.) Pers.

2018 ◽  
Vol 25 (4) ◽  
Author(s):  
Fabrício Palla Teixeira ◽  
José Marcio Rocha Faria ◽  
Wilson Vicente Souza Pereira ◽  
Anderson Cleiton José
Keyword(s):  
Desiccation Tolerance ◽  
Sesbania Virgata
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