The Fluorescence Image of Portable System Enhanced by Asynchronous Trigger UV-LED Excitation

2013 ◽  
Vol 284-287 ◽  
pp. 543-549
Author(s):  
Wen Hong Wu ◽  
Kuo Cheng Huang ◽  
Yi Ju Chen ◽  
Han Chao Chang ◽  
Chung Hsing Chang
Keyword(s):  
Relative Intensity ◽  
Near Infrared ◽  
Uv Light ◽  
Frame Rate ◽  
Stray Light ◽  
Infrared Light ◽  
Biochemical Effect ◽  
Fluorescence Image ◽  
Led Light ◽  
Uv Led

Fluorescence is widely used to detect the biochemical effect and some substance containing certain dye. In general, the formation of fluorescent reaction is that an organism or dye, excited by UV light, emits a specific frequency of light; the light is usually a visible or near infrared light. Practically, the fluorescence of object can be excited by continued UV light, but the contrast and sharpness of fluorescence image decrease readily with stray light from the surrounding. In this study, we connect a trigger LED light module to a portable camera system and to perform the fluorescence inspection. When the fluorescent object is excited by asynchronous trigger UV-LED light, the extra intensity of fluorescence can be obtained. In the experiment of security organic dye (BL-ORT), the relative intensity of fluorescence acquired by 30 fps CCD can be increased by more than 11 %. In addition, when the fluorescent dye (chlorine e6) is injected into the tail vein of nude mouse, if its tail excited by the 375nm asynchronous trigger and continuous UV-LED are processed, the average relative intensity is 56.5 % and 49 %, respectively. Therefore, an added relative fluorescence of 15.3 % can be obtained from asynchronous triggering method. Furthermore, the ratio of extra intensity increases with the increase of frame rate of camera.

scholarly journals Photothermal Therapy Using Gold Nanorods and Near-Infrared Light in a Murine Melanoma Model Increases Survival and Decreases Tumor Volume

2014 ◽  
Vol 2014 ◽  
pp. 1-8 ◽  
Author(s):  
Mary K. Popp ◽  
Imane Oubou ◽  
Colin Shepherd ◽  
Zachary Nager ◽  
Courtney Anderson ◽  
...  
Keyword(s):  
Light Source ◽  
Gold Nanorods ◽  
Photothermal Therapy ◽  
Near Infrared ◽  
Tumor Volume ◽  
Infrared Light ◽  
Led Light ◽  
Murine Melanoma ◽  
Nir Light ◽  
Melanoma Model

Photothermal therapy (PTT) treatments have shown strong potential in treating tumors through their ability to target destructive heat preferentially to tumor regions. In this paper we demonstrate that PTT in a murine melanoma model using gold nanorods (GNRs) and near-infrared (NIR) light decreases tumor volume and increases animal survival to an extent that is comparable to the current generation of melanoma drugs. GNRs, in particular, have shown a strong ability to reach ablative temperatures quickly in tumors when exposed to NIR light. The current research tests the efficacy of GNRs PTT in a difficult and fast growing murine melanoma model using a NIR light-emitting diode (LED) light source. LED light sources in the NIR spectrum could provide a safer and more practical approach to photothermal therapy than lasers. We also show that the LED light source can effectively and quickly heatin vitroandin vivomodels to ablative temperatures when combined with GNRs. We anticipate that this approach could have significant implications for human cancer therapy.

scholarly journals Enhanced UV Light Emission by Core-Shell Upconverting Particles Powering up TiO2 Photocatalysis in Near-Infrared Light

Catalysts ◽  
2020 ◽  
Vol 10 (2) ◽  
pp. 232
Author(s):  
Agnieszka Jarosz-Duda ◽  
Paulina O’Callaghan ◽  
Joanna Kuncewicz ◽  
Przemysław Łabuz ◽  
Wojciech Macyk
Keyword(s):  
Near Infrared ◽  
Light Emission ◽  
Uv Light ◽  
Protective Layer ◽  
Upconversion Emission ◽  
High Photocatalytic Activity ◽  
Infrared Light ◽  
Core Shell ◽  
The Core ◽  
Surface Luminescence

The core-shell NaYb0.99F4:Tm0.01@NaYF4 upconverting particles (UCPs) with a high UV emission to apply in NIR-driven photocatalysis were synthesized. The influence of the Yb3+ doping concentration in NaYxF4:Yb0.99−xTm0.01 core particles, and the role of the NaYF4 shell on the upconversion emission intensity of the UCPs were studied. The absorption of NIR light by the obtained UCPs was maximized by increasing the Yb3+ concentration in the core, reaching the maximum for Y3+-free particles (NaYb0.99F4:Tm0.01). Additionally, covering the NaYb0.99F4:Tm0.01 core with a protective layer of NaYF4 minimized the surface luminescence quenching, which significantly improved the efficiency of upconversion emission. The high intensity of the UV light emitted by the NaYb0.99F4:Tm0.01@NaYF4 under NIR irradiation resulted in a high photocatalytic activity of TiO2 (P25) mixed with the synthesized material.

scholarly journals A Visible and Near-Infrared Light Activatable Diazo-Coumarin Probe for Fluorogenic Protein Labeling in Living Cells

2020 ◽  
Author(s):  
Sheng-Yao Dai ◽  
Dan Yang
Keyword(s):  
Near Infrared ◽  
Uv Light ◽  
Cell Types ◽  
Living Cells ◽  
Infrared Light ◽  
Fluorescence Labeling ◽  
Insertion Reaction ◽  
Selective Labeling ◽  
Near Infrared Light ◽  
Endogenous Protein

Chemical modification of proteins in living cells permits valuable glimpses into the molecular interactions that underpin dynamic cellular events. While genetic engineering methods are often preferred, selective labeling of endogenous proteins in a complex intracellular milieu with chemical approaches represents a significant challenge. In this study, we report novel diazo-coumarin compounds that can be photo-activated by visible (430‒490 nm) and near-infrared light (800 nm) irradiation to photo-uncage reactive carbene intermediates, which could subsequently undergo insertion reaction with concomitant fluorescence “turned-on”. With these new molecules in hand, we have developed a new approach for rapid, selective and fluorogenic labeling of endogenous protein in living cells. By using CA-II and eDHFR as model proteins, we demonstrated that subcellular localization of proteins can be precisely visualized by live-cell imaging and protein levels can be reliably quantified in multiple cell types using flow cytometry. Dynamic protein regulations such as hypoxia induced CA-IX accumulation can also be detected. In addition, by two-photon excitation with an 800 nm laser, cell-selective labeling can also be achieved with spatially controlled irradiation. Our method circumvents the cytotoxicity of UV light and obviates the need for introducing external reporters with “click chemistries”. We believe that this approach of fluorescence labeling of endogenous protein by bioorthogonal photo-irradiation opens up exciting opportunities for discoveries and mechanistic interrogation in chemical biology.

scholarly journals Nanotechnology for photodynamic therapy: a perspective from the Laboratory of Dr. Michael R. Hamblin in the Wellman Center for Photomedicine at Massachusetts General Hospital and Harvard Medical School

2015 ◽  
Vol 4 (4) ◽  
Author(s):  
Michael R. Hamblin ◽  
Long Y. Chiang ◽  
Shanmugamurthy Lakshmanan ◽  
Ying-Ying Huang ◽  
Maria Garcia-Diaz ◽  
...  
Keyword(s):  
Photodynamic Therapy ◽  
Opposite Effect ◽  
Near Infrared ◽  
Uv Light ◽  
Massachusetts General Hospital ◽  
Drug Carriers ◽  
Infrared Light ◽  
Great Role ◽  
Resonance Enhancement ◽  
Walled Carbon Nanotubes

AbstractThe research interests of the Hamblin Laboratory are broadly centered on the use of different kinds of light to treat many different diseases. Photodynamic therapy (PDT) uses the combination of dyes with visible light to produce reactive oxygen species and kill bacteria, cancer cells and destroy unwanted tissue. Likewise, UV light is also good at killing especially pathogens. By contrast, red or near-infrared light can have the opposite effect, to act to preserve tissue from dying and can stimulate healing and regeneration. In all these applications, nanotechnology is having an ever-growing impact. In PDT, self-assembled nano-drug carriers (micelles, liposomes, etc.) play a great role in solubilizing the photosensitizers, metal nanoparticles can carry out plasmon resonance enhancement, and fullerenes can act as photosensitizers, themselves. In the realm of healing, single-walled carbon nanotubes can be electrofocused to produce nano-electonic biomedical devices, and nanomaterials will play a great role in restorative dentistry.

scholarly journals Fabrication of Upconverting Hybrid Nanoparticles for Near-Infrared Light Triggered Drug Release

2014 ◽  
Vol 2014 ◽  
pp. 1-9 ◽  
Author(s):  
Ranran Zhang ◽  
Risheng Yao ◽  
Binbin Ding ◽  
Yuxin Shen ◽  
Shengwen Shui ◽  
...  
Keyword(s):  
Drug Release ◽  
Near Infrared ◽  
Uv Light ◽  
Nile Red ◽  
Controlled Drug Release ◽  
Hybrid Nanoparticles ◽  
Infrared Light ◽  
Nir Light ◽  
Triggered Drug Release ◽  
Model Drug

Low tissue penetration and harmful effects of (ultraviolet) UV or visible light on normal tissue limit exploiting nanocarriers for the application of light-controlled drug release. Two strategies may solve the problem: one is to improve the sensitivity of the nanocarriers to light to decrease the radiation time; the other one is using more friendly light as the trigger. In this work, we fabricated a core-shell hybrid nanoparticle with an upconverting nanoparticle (UCNP) as the core and thermo- and light-responsive block copolymers as the shell to combine the two strategies together. The results indicated that the sensitivity of the block copolymer to light could be enhanced by decreasing the photolabile moieties in the polymer, and the UCNP could transfer near-infrared (NIR) light, which is more friendly to tissue and cell, to UV light to trigger the phase conversion of the block polymersin situ. Using Nile Red (NR) as the model drug, the hybrid nanoparticles were further proved to be able to act as carriers with the character of NIR triggered drug release.

Fluorescence image excited by a scanning UV-LED light

2013 ◽  
Vol 20 (2) ◽  
pp. 198-201 ◽  
Author(s):  
Hsin-Yi Tsai ◽  
Yi-Ju Chen ◽  
Kuo-Cheng Huang
Keyword(s):  
Fluorescence Image ◽  
Led Light ◽  
Uv Led

scholarly journals Photocatalytic Oxidative Degradation of Carbamazepine by TiO2 Irradiated by UV Light Emitting Diode

Catalysts ◽  
2020 ◽  
Vol 10 (5) ◽  
pp. 540 ◽  
Author(s):  
Zhilin Ran ◽  
Yuanhang Fang ◽  
Jian Sun ◽  
Cong Ma ◽  
Shaofeng Li
Keyword(s):  
Photocatalytic Degradation ◽  
Uv Light ◽  
Reaction System ◽  
Reference Method ◽  
Degradation Efficiency ◽  
Mixed Phase ◽  
Light Emitting ◽  
Led Light ◽  
Irradiation Intensity ◽  
Uv Led

Here, ultraviolet light-emitting diodes (UV-LED) combined with TiO2 was used to investigate the feasibility of carbamazepine (CBZ) degradation. The effects of various factors, like crystal form of the catalyst (anatase, rutile, and mixed phase), mass concentration of TiO2, wavelength and irradiation intensity of the UV-LED light source, pH of the reaction system, and coexisting anions and cations, on the photocatalytic degradation of CBZ were studied. The mixed-phase (2.8 g/L) showed the best degradation efficiency at 365 nm among three kinds of TiO2, wherein CBZ (21.1 µM) was completely oxidized within 1 h. The results of batch experiments showed that: (i) CBZ degradation efficiency under UV-LED light at 365 nm was higher than 275 nm, due to stronger penetrability of 365 nm light in solution. (ii) The degradation efficiency increased with increase in irradiation intensity and pH, whereas it decreased with increase in initial CBZ concentration. (iii) The optimal amount of mixed-phase TiO2 catalyst was 2.8 g/L and excessive catalyst decreased the rate. (iv) The co-existence of CO32−, HCO3−, and Fe3+ ions in water significantly accelerated the degradation rate of photocatalytic CBZ, whereas Cu2+ ions strongly inhibited the degradation process of CBZ. ·OH was found to be the main active species in the UV-LED photocatalytic degradation of CBZ. UV-LED is more environmentally friendly, energy efficient, and safer, whereas commercial TiO2 is economical and readily available. Therefore, this study provides a practically viable reference method for the degradation of pharmaceuticals and personal care products (PPCPs).

Enhanced Photocatalytic Activity of Cu1.8Se/CuAgSe for Organic Pollutants under Visible and Near-Infrared Light

2018 ◽  
Vol 281 ◽  
pp. 825-829
Author(s):  
Li Na Qiao ◽  
Ming Rui Tang ◽  
Yong Yun Gang ◽  
Hao Min Xu ◽  
Yuan Hua Lin
Keyword(s):  
Photocatalytic Activity ◽  
Room Temperature ◽  
Near Infrared ◽  
Uv Light ◽  
Infrared Light ◽  
Precipitation Method ◽  
Near Infrared Light ◽  
Step Process ◽  
Ion Exchange Reaction ◽  
Light Region

The Cu1.8Se/CuAgSe nanostructure was synthesized by a simple two-step process. Starting with the template of cubic Cu1.8Se nanoplate by precipitation method, Cu1.8Se/CuAgSe nanostructure and ternary CuAgSe were prepared through a rapid ion exchange reaction using various amount of AgNO3 at room temperature. The as-prepared samples were analyzed by XRD, SEM and DRS. It was found that Cu1.8Se/CuAgSe heterostructure and the pure CuAgSe phase were formed without changing the morphology, and these samples had efficient light absorption from UV light to near-infrared light region. Photocatalytic properties of these samples were evaluated by the degradation of Congo red under visible and near-infrared light. The Cu1.8Se/CuAgSe nanostructure showed enhanced photocatalytic activity due to the lower recombination of charge-carrier in the photodegradation process.

Effect of Spectral Range in Surface Inactivation of Listeria innocua Using Broad-Spectrum Pulsed Light

2007 ◽  
Vol 70 (4) ◽  
pp. 909-916 ◽  
Author(s):  
SARAH E. WOODLING ◽  
CARMEN I. MORARU
Keyword(s):  
Cell Death ◽  
Near Infrared ◽  
Uv Light ◽  
Listeria Innocua ◽  
Infrared Light ◽  
Plate Count ◽  
Pulsed Light ◽  
Full Spectrum ◽  
Near Infrared Light ◽  
Probable Number

Pulsed light (PL) treatment is an alternative to traditional thermal treatment that has the potential to achieve several log-cycle reductions in the concentration of microorganisms. One issue that is still debated is related to what specifically causes cell death after PL treatments. The main objective of this work was to elucidate which portions of the PL range are responsible for bacterial inactivation. Stainless steel coupons with controlled surface properties were inoculated with a known concentration of Listeria innocua in the stationary growth phase and treated with 1 to 12 pulses of light at a pulse rate of 3 pulses per s and a pulse width of 360 μs. The effects of the full spectrum (λ= 180 to 1,100 nm) were compared with the effects obtained when only certain regions of UV, visible, and near-infrared light were used. The effectiveness of the treatments was determined in parallel by the standard plate count and most-probable-number techniques. At a fluence of about 6 J/cm2, the full-spectrum PL treatment resulted in a 4.08-log reduction of L. innocua on a Mill finish surface, the removal of λ< 200 nm diminished the reduction to only 1.64 log, and total elimination of UV light resulted in no lethal effects on L. innocua. Overwhelmingly, the portions of the PL spectrum responsible for bacterial death are the UV-B and UV-C spectral ranges (λ< 300 nm), with some death taking place during exposure to UV-A radiation (300 <λ< 400 nm) and no observable death upon exposure to visible and near-infrared light (λ> 400 nm). This work provides additional supporting evidence that cell death in PL treatment is due to exposure to UV light. Additionally, it was shown that even a minor modification of the light path or the UV light spectrum in PL treatments can have a significant negative impact on the treatment intensity and effectiveness.

Application of lanthanide-doped upconversion nanoparticles for cancer treatment: a review

Nanomedicine ◽  
2021 ◽  
Author(s):  
Yu-Qi Liu ◽  
Li-Ying Qin ◽  
Hong-Jiao Li ◽  
Yi-Xi Wang ◽  
Rui Zhang ◽  
...  
Keyword(s):  
Cancer Treatment ◽  
Photothermal Therapy ◽  
Near Infrared ◽  
Uv Light ◽  
Infrared Light ◽  
Upconversion Nanoparticles ◽  
Tissue Penetration ◽  
Deep Tissue ◽  
Chemotherapy Drugs ◽  
New Ideas

With the excellent ability to transform near-infrared light to localized visible or UV light, thereby achieving deep tissue penetration, lanthanide ion-doped upconversion nanoparticles (UCNP) have emerged as one of the most striking nanoscale materials for more effective and safer cancer treatment. Up to now, UCNPs combined with photosensitive components have been widely used in the delivery of chemotherapy drugs, photodynamic therapy and photothermal therapy. Applications in these directions are reviewed in this article. We also highlight microenvironmental tumor monitoring and precise targeted therapies. Then we briefly summarize some new trends and the existing challenges for UCNPs. We hope this review can provide new ideas for future cancer treatment based on UCNPs.

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