Determination of Antioxidant Activities and Total Phenolic Contents of Three Cyanobacterial Species

2014 ◽  
Vol 625 ◽  
pp. 826-829 ◽  
Author(s):  
Hamdy Elsayed Ahmed Ali ◽  
Sanaa Mahmoud Metwally Shanab ◽  
Mervat Aly Mohamed Abo-State ◽  
Emad Ahmed Ahmed Shalaby ◽  
Usama Mohamed Nour El Demerdash ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Activities ◽  
Water Extract ◽  
Correlation Coefficients ◽  
Ethanolic Extract ◽  
Natural Antioxidants ◽  
Total Phenolic ◽  
Phenolic Contents ◽  
Cyanobacterial Species ◽  
Dpph Scavenging

The antioxidant activity and total phenolic content of different extracts (hexane, chloroform, ethyl acetate, ethanol (70%) and water) of three tested cyanobacterial species (Phormidium sp., Oscillatoria sp. and Nostoc muscorum ) were evaluated, using 2,2 ́-diphenyl-1-picrylhydrazyl (DPPH) scavenging method and Folin–Ciocalteu method, respectively. The ethanolic extract of Oscillatoria sp. possessed the highest antioxidant activity (69.13±0.35 %) and therefore could be potential source of natural antioxidants. The correlation coefficients between the antioxidant activities and the phenolic contents were very small in most of extracts (R2 = 0.0291-0.2934). However, the water extract demonstrated relatively higher value (R2= 0.7446). Thus, phenolic compounds may not be considered the major contributors for the antioxidant activities of these cyanobacterial species. So, other antioxidant substances may be present in the extract and share in this activity.

Screening of Microalgae for Antioxidant Activities, Carotenoids and Phenolic Contents

2014 ◽  
Vol 625 ◽  
pp. 156-159 ◽  
Author(s):  
Hamdy Elsayed Ahmed Ali ◽  
Sanaa Mahmoud Metwally Shanab ◽  
Mervat Aly Mohamed Abo-State ◽  
Emad Ahmed Ahmed Shalaby ◽  
Usama Eldmerdash ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Activities ◽  
Scenedesmus Obliquus ◽  
Ethanolic Extract ◽  
Natural Antioxidants ◽  
Carotenoid Content ◽  
Total Phenolic ◽  
Microalgal Biomass ◽  
Phenolic Contents ◽  
Dpph Scavenging

Ten different microalgal species were screened for their antioxidant activity using 2,2 ́-diphenyl-1-picrylhydrazyl (DPPH) scavenging assay, both total phenolic and carotenoid contents were determined. The ethanolic extract ofOscillatoriasp. showed the highest antioxidant activity (69.1 %), while the greenChlorellasp. showed higher phenolic content (39.1 mg GAE g-1dry wt.) andScenedesmus obliquusrecorded higher carotenoid content (3.73 mg L-1), than cyanobacterial species Microalgal biomass plays a great role as a potential source of natural antioxidants, not only due to the carotenoids but also the phenolic compounds.

scholarly journals In vitro antioxidant activity of stem bark of Trichilia catigua Adr. Juss

2012 ◽  
Vol 62 (3) ◽  
pp. 371-382 ◽  
Author(s):  
Jean Paul Kamdem ◽  
Sílvio Terra Stefanello ◽  
Aline Augusti Boligon ◽  
Caroline Wagner ◽  
Ige Joseph Kade ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Activities ◽  
Ethanolic Extract ◽  
Stem Bark ◽  
Total Phenolic ◽  
Dependent Manner ◽  
Concentration Dependent Manner ◽  
High Concentrations ◽  
Dpph Scavenging

Antioxidant activity of the ethanolic extract and fractions from the stem bark of T. catigua was investigated. IC50 (for DPPH scavenging) by T. catigua varied from 9.17 ± 0.63 to 76.42 ± 5.87 mg mL-1 and total phenolic content varied from 345.63 ± 41.08 to 601.27 ± 42.59 mg GAE g-1 of dry extract. Fe2+-induced lipid peroxidation was significantly reduced by the ethanolic extract and fractions. Mitochondrial Ca2+-induced dichlorofluorescein oxidation was significantly reduced by the ethanolic extract in a concentration-dependent manner. Ethanolic extract reduced mitochondrial Dym only at high concentrations (40-100 mg mL-1), which indicates that its toxicity does not overlap with its antioxidant effects. Results suggest involvement of antioxidant activities of T. catigua in its pharmacological properties.

scholarly journals Antioxidant activity of Ruscus species from Serbia: Potential new sources of natural antioxidants

2016 ◽  
Vol 70 (1) ◽  
pp. 99-106 ◽  
Author(s):  
Violeta Jakovljevic ◽  
Jasmina Milicevic ◽  
Gorica Ðelic ◽  
Miroslav Vrvic
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Ethyl Acetate Extract ◽  
Reducing Power ◽  
Ethanolic Extract ◽  
Natural Antioxidants ◽  
Total Phenolic ◽  
Scavenging Activity ◽  
Flavonoid Content ◽  
Dpph Scavenging

The antioxidant activity, total phenolic and flavonoid content of ethanolic, acetone and ethyl acetate extracts of Ruscus hypoglossum L. and Ruscus aculeatus L. (aerial parts) from Serbia were investigated in this paper. The best total antioxidant capacity (23.329 ?g AA g-1) and the highest DPPH scavenging activity (IC50 = 182.54 ?g mL-1) were found in acetone and ethyl acetate extract of R. aculeatus L. Ethanolic extract of R. hypoglossum L. showed the highest ABTS radical cation scavenging activity (IC50 = 3.04 ?g mL-1) as well as reducing power (IC50 = 0.143 ?g mL-1). The best inhibitory activity against lipid peroxidation (IC50 =651 ?g mL-1) and the best ferrous ion chelating ability (IC50 = 110 ?g mL-1) were found in acetone and ethyl acetate extract of R. hypoglossum L. The highest total phenolic (8.569 mg GAE g-1) and flavonoid content (0.136 mg RU g-1) were found in ethanolic and acetone extract of R. hypoglossum L. and R. aculeatus L, respectively.

SKRINING FITOKIMIA DAN UJI AKTIVITAS ANTIOKSIDAN DAN ANTITUMOR 20 TUMBUHAN OBAT ASAL KABUPATEN MANOKWARI

2008 ◽  
Vol 7 (1) ◽  
Author(s):  
Bimo Budi Santoso
Keyword(s):  
Antioxidant Activity ◽  
Medicinal Plants ◽  
Antitumor Activity ◽  
Traditional Method ◽  
Extraction Efficiency ◽  
Phenolic Content ◽  
Antioxidant Activities ◽  
Total Phenolic ◽  
Phenolic Contents ◽  
Total Phenolic Contents

<strong><em>Flavanoid, steroid, and terpenoid contents of 20 Manokwari medicinal plants were analyzed.  The antioxidant activity, total phenolic contents and antitumor activity of 20 Manokwari medicinal plants were also evaluated. The result shows that 83% positif to flavanoid test, 59% positif to terpenoid test and only 25% positif steroid. Antioxidant activity and total phenolic contents evaluated using Ferric Thiocianate (FTC) and Folin-Ciocalteu methods respectively. Antioxidant activity and total phenolic contents of medicinal plants were extracted by the traditional method, boiling in water and also in 80% methanol. Twenty plants evaluated in both exstracts have significantly varies of antioxidant activities and phenolic contents,  A significant and linier correlation coefficient between the antioxidant activity and the total phenolic content was found in both aqueous (R<sup>2</sup>= 0,77) and methanol (R<sup>2</sup> = 0,85). Antitumor activity was tested using cell maurine P-388 and only 2 of medicinal plants are active to inhibit cell maurine P-388. Comparing extraction efficiency of the two methods, the methanol extracted phenolic compounds more efficiently, and antioxidant activity of the extract was higher.</em></strong>

scholarly journals Antioxidant Activity of Averrhoa bilimbi Linn. Leaves Extract Using Two Different Types of Solvents

2021 ◽  
Author(s):  
Raja Nurul Ashiqin Raja Arifin ◽  
Juliana Jumal
Keyword(s):  
Antioxidant Activity ◽  
Gallic Acid ◽  
Skin Diseases ◽  
Water Extract ◽  
Ethanolic Extract ◽  
Natural Antioxidant ◽  
Aluminium Chloride ◽  
Total Phenolic ◽  
Different Types ◽  
Gallic Acid Equivalent

Exposed to the pollution has led to generation of reactive oxygen species (ROS) in human skin. ROS generated cause many skin diseases such as skin-aging, inflammation, melanogenesis and skin cancer. ROS is a family of oxygen-based free radicals that contains or capable of producing an unpaired electron. Antioxidant is a molecule that can inhibit the reaction of free radical from ROS by donating its electron. Averrhoa bilimbi Linn. (AVBL) is one of the potent natural antioxidant belongs to the group of Oxalidaceae which can be widely found in Asia including Malaysia. Traditionally, this plant has been used to treat many diseases such as cough, itchiness, pimple, fever and inflammation. As a result, much attention has been directed towards the studies regarding the potential of this plant in treating disease. The present study was undertaken to assess the antioxidant activity of AVBL leaves extract. The AVBL leaves were extracted using sonicator with ethanol and distilled water as two different types of solvent. The total phenolic content (TPC) and flavonoid content (TFC) of this study were determined by using Folin-Ciocalteu reagent and aluminium chloride colometric assay. Antioxidant activity of the plant extract was tested using 2,2- diphenyl-1-picrylhydrazyl (DPPH) and ferric reducing power (FRAP). From the analyses, water extract of AVBL possessed greater extraction yield (11.231%) as compared to ethanolic extract (5.358 %). However, ethanolic extract of AVBL leaves revealed higher result of TPC (126.4±0.35 mg/g gallic acid equivalent), TFC (32.80±0.37 mg/g quercetin equivalent), DPPH (0.0019±0.0003) and FRAP (41.81±0.45 mg/g gallic acid equivalent). The results of TPC and TFC have strongly positive correlation with antioxidant capacity (r = 1). Thus, it can be concluded that this plant is a potent source of natural antioxidant.

scholarly journals Assessment of in vitro Antioxidant and Antidiabetic Capacities of Medlar (Mespilus germanica)

2018 ◽  
Vol 47 (2) ◽  
pp. 384-389
Author(s):  
Sebnem Selen ISBILIR ◽  
Sevilay Inal KABALA ◽  
Hulya YAGAR
Keyword(s):  
Antioxidant Activity ◽  
Leaf Extract ◽  
Antioxidant Activities ◽  
Inhibitory Effect ◽  
Total Phenolic ◽  
Flower Bud ◽  
Ethanol Extracts ◽  
Dpph Scavenging ◽  
Β Carotene

The objective of the current study was to evaluate the antioxidant activity and enzyme inhibitory effect of different parts of medlar including fruit, leaf and flower bud by using various in vitro methods, and also determination of total phenolic and flavonoid content in the samples. Ethanol extracts of medlar parts were prepared and their antioxidant activities were determined using 1,1-diphenyl-2-picryl-hydrazil (DPPH•) scavenging and β-carotene bleaching methods. The leaf extract showed the strongest antioxidant activity. DPPHradical scavenging activity was in the order of BHA > leaf > bud > fruit. This ordering was the same for β-carotene bleaching activity, tocopherol > leaf > bud > fruit. The highest total phenolic (60.3 ± 1.69 mg GAE g-1 extract) and flavonoid (14.77 ± 1.15 mg QE g-1 extract) content were determined in leaf extract. For possible antidiabetic effects of extracts, α-amylase and α-glucosidase inhibitory activities were investigated, the bud extract showed the highest inhibition activities among the all extracts.

scholarly journals COMPARATIVE STUDY ON THE ANTIOXIDANT ACTIVITIES OF METHANOLIC AND AQUEOUS EXTRACTS OF TERMINALIA BELLERICA

2019 ◽  
pp. 103-110
Author(s):  
RASNA GUPTA ◽  
RAM LAKHAN SINGH ◽  
ANKIT GUPTA
Keyword(s):  
Antioxidant Activities ◽  
Natural Antioxidants ◽  
Total Phenolic ◽  
Aqueous Extracts ◽  
Fruit Pulp ◽  
Terminalia Bellerica ◽  
Phenolic Contents ◽  
Total Phenolic Contents ◽  
Methanolic Extracts ◽  
Better Than

Objective: The present study was undertaken to investigate the antioxidative activity of fruit pulp, seed and bark of T. bellerica. Methods: Methanol (70%) and water were used for the extraction, and the respective extracts were analyzed for total phenolic contents along with the antioxidant activities through different assays. Results: Amongst the tested extracts, methanolic extracts were found to be better than aqueous with the highest total phenolic contents (TPC). Methanolic fruit pulp showed lower IC50 for free radical (118.7 µg/ml), superoxide anion radical (77.65 µg/ml) and hydroxyl radical (73.76 µg/ml) scavenging activities. Methanolic fruit pulp also showed lower IC50 for lipid peroxidation (115.6 µg/ml) and ferric thiocyanate (184.98 µg/ml) assay than aqueous extracts in comparison to standard quercetin. Further, correlation between TPC and antioxidant studies revealed that phenolics are mainly responsible for antioxidant activity of T. bellerica. Conclusion: The current study suggests that the methanolic extract of T. bellerica could be a potential source of natural antioxidants for food and pharmaceutical companies.

scholarly journals Loranthus pulverulentus: A Potent Source of Natural Antioxidants and Alternative Medicine

2013 ◽  
Vol 2013 ◽  
pp. 1-7 ◽  
Author(s):  
Muhammad Asam Raza ◽  
Rukhsana Kausar ◽  
Faraz Ali Rana ◽  
Muhammad Danish ◽  
Durre Shahwar ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Natural Antioxidants ◽  
Stem Bark ◽  
Antioxidant Potential ◽  
Total Phenolic ◽  
Strong Correlations ◽  
Phenolic Contents ◽  
Total Phenolic Contents ◽  
Total Antioxidant

This study was designed to evaluate the antioxidant potential ofLoranthus pulverulentus. Stem bark, leaves, and seeds ofLoranthus pulverulentuswere extracted in methanol:water (90 : 10) and partitioned with n-hexane, chloroform, ethyl acetate, and n-butanol successively using partition chromatography. Total phenolic contents and antioxidant potential were checked using standard protocols. Total phenolic contents of all extracts were determined, using Folin–Ciocalteu reagent, and ranged between 151 ± 2.1 and 396 ± 1.6 for stem bark, 137 ± 0.9 and 430 ± 2.2 for, and 39 ± 0.6 and 231 ± 1.7 for seeds. The antioxidant potential of extracts was evaluated; namely, DPPH, FRAP, and total antioxidant models. The ethyl acetate extract of stem-bark, leaves, and seeds showed the highest activity in DPPH (94.5 ± 2.1%, 96.30 ± 0.9%, and 92.30 ± 1.1%, IC5015.9 ± 0.5 μg, 14.5 ± 0.8, and 102.7 ± 1.3, resp.), FRAP (7.7 ± 0.6, 7.5 ± 0.7 and 6.6 ± 0.7, resp.), and total antioxidant (0.95 ± 0.09, 1.19 ± 0.09, and 0.686 ± 0.08, resp.). Strong correlations were observed between total phenols versus total antioxidant activity, DPPH, and FRAP withR2values ranging from 0.8185 to 0.9951 (stem-bark), 0.6728 to 0.8648 (leaves), and 0.8658 to 0.9910 (seed) which indicated that phenolic contents are the major constituents responsible for antioxidant activity.

scholarly journals Efficacy of N-Methyl-N-Nitrosourea Mutation on Physicochemical Properties, Phytochemicals, and Momilactones A and B in Rice

2019 ◽  
Vol 11 (23) ◽  
pp. 6862 ◽  
Author(s):  
Kifayatullah Kakar ◽  
Tran Dang Xuan ◽  
Nguyen Van Quan ◽  
Imran Khan Wafa ◽  
Hoang-Dung Tran ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Functional Foods ◽  
Grain Quality ◽  
Antioxidant Activities ◽  
Total Phenolic ◽  
Rice Grain ◽  
Rice Cultivars ◽  
Phenolic Contents ◽  
Phytochemical Compounds ◽  
Beneficial Traits

Attempts regarding the improvement and development of novel rice with better quality and higher productivity have been increasing. Among approaches, mutation is a direct alteration on the genome and considered as one of the most beneficial routes to acquire new beneficial traits in rice. An experiment was carried out to explore the effects of N-methyl-N-nitrosourea (MNU) mutation on the antioxidant activities, phytochemical compounds, and momilactones A (MA) and B (MB) in rice. Two rice cultivars, K1 (an original cultivar DT84) and K2 (mutated DT84), were examined. Antioxidant activities, phenolic compounds, and momilactones of the rice grain, husk, and straw portions were measured and quantified. Antioxidant activities were higher in grain and straw of K2, whereas K1 showed greater antioxidant activity in rice husk. Additionally, K2 displayed higher total phenolic contents (TPC) in grain and straw as well as lower of it in the husk, but these variations significantly differed only in the straw portion. An increase in total flavonoid contents (TFC) was observed in the husk of K1, while K2 significantly enhanced TFC in straw. Both MA and MB, two compounds obtaining antidiabetes, anticancer, antimicrobial, antigout, and antiobesity properties, were detected and quantified in grain, husk, and straw of K1 and K2 samples. Generally, the contents of MA were higher than MB in all tested portions of rice crop. MA and MB were higher in straw followed by those in husk and grain, respectively. K2 contained higher amounts of MA and MB in straw and husk, but lower contents in grain compared with those in K1. This study illustrates that MNU mutation can improve grain quality and enhance bioactive compounds in straw, husk, and grain of rice. This approach has the potential to develop functional foods from rice, and therefore help farmers in developing countries to improve value in rice production.

Antioxidant Properties of Ethanolic Extract from Ramulus mori (Sangzhi)

2009 ◽  
Vol 15 (5) ◽  
pp. 435-444 ◽  
Author(s):  
Zuofa Zhang ◽  
Jie Jin ◽  
Liangen Shi
Keyword(s):  
Antioxidant Activity ◽  
Superoxide Radical ◽  
Antioxidant Activities ◽  
Antioxidant Properties ◽  
Reducing Power ◽  
Ethanolic Extract ◽  
Experimental Models ◽  
Hplc Method ◽  
Total Phenolic ◽  
Dependent Manner

The antioxidant properties and total phenolic contents of four fractions of ethanolic extract from Ramulus mori were examined. Various experimental models including superoxide radical, hydroxyl radical, 1,1-diphenyl-2-picrylhydrazyl radicals (DPPH) scavenging activity, metal chelating activity, and reducing power were used for characterization of their antioxidant activity. The four fractions showed various degrees of efficacy in each assay in a dose-dependent manner. The third fraction with the highest amount of total phenolics was the most potent antioxidant in all assays used. In addition, the most powerful compound (oxyresveratrol) was isolated and identified followed by on-line HPLC method and characterized by different spectral analysis. Oxyresveratrol exhibited impressive antioxidant activities in scavenging the superoxide radical, hydroxide radical, and DPPH. On the basis of the results obtained, Ramulus mori may serve as a potential source of natural antioxidant due to its significant antioxidant activity and oxyresveratrol may be the most powerful antioxidant in ethanolic extracts of Ramulus mori.

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