Biofilm Formation of Sulfobacillus thermosulfidooxidans on Pyrite in the Presence of Leptospirillum ferriphilum

2015 ◽  
Vol 1130 ◽  
pp. 141-144 ◽  
Author(s):  
Qian Li ◽  
Rui Yong Zhang ◽  
Beate A. Krok ◽  
Mario Vera ◽  
Wolfgang Sand
Keyword(s):  
Biofilm Formation ◽  
Physical Contact ◽  
Attachment Behavior ◽  
Initial Attachment ◽  
Preliminary Results ◽  
Leptospirillum Ferriphilum

In this study, initial attachment to and biofilm formation of Sulfobacillusthermosulfidooxidans DSM 9293T on pyrite in the presence of Leptospirillumferriphilum DSM 14647T were investigated. Interactions of S.thermosulfidooxidansT and L.ferriphilumT were studied by means of monitoring attachment behavior and biofilm formation on pyrite. Our preliminary results showed that 1): Pre-established biofilms of L.ferriphilumT had effects on attachment of S. thermosulfidooxidansT to pyrite; 2): physical contact between cells of L. ferriphilumT and S. thermosulfidooxidansT on pyrite were visible 3): Pyrite leaching by cells of S. thermosulfidooxidansT was inhibited by the presence of inactive cells of L.ferriphilumT.

scholarly journals Altering the Ratio of Phenazines in Pseudomonas chlororaphis (aureofaciens) Strain 30-84: Effects on Biofilm Formation and Pathogen Inhibition

2008 ◽  
Vol 190 (8) ◽  
pp. 2759-2766 ◽  
Author(s):  
V. S. R. K. Maddula ◽  
E. A. Pierson ◽  
L. S. Pierson
Keyword(s):  
Fungal Pathogen ◽  
Biofilm Formation ◽  
Flow Cell ◽  
Gaeumannomyces Graminis ◽  
Pseudomonas Chlororaphis ◽  
Wild Type ◽  
Initial Attachment ◽  
Pathogen Inhibition ◽  
Biofilm Development ◽  
Derivatives Of

ABSTRACT Pseudomonas chlororaphis strain 30-84 is a plant-beneficial bacterium that is able to control take-all disease of wheat caused by the fungal pathogen Gaeumannomyces graminis var. tritici. The production of phenazines (PZs) by strain 30-84 is the primary mechanism of pathogen inhibition and contributes to the persistence of strain 30-84 in the rhizosphere. PZ production is regulated in part by the PhzR/PhzI quorum-sensing (QS) system. Previous flow cell analyses demonstrated that QS and PZs are involved in biofilm formation in P. chlororaphis (V. S. R. K. Maddula, Z. Zhang, E. A. Pierson, and L. S. Pierson III, Microb. Ecol. 52:289-301, 2006). P. chlororaphis produces mainly two PZs, phenazine-1-carboxylic acid (PCA) and 2-hydroxy-PCA (2-OH-PCA). In the present study, we examined the effect of altering the ratio of PZs produced by P. chlororaphis on biofilm formation and pathogen inhibition. As part of this study, we generated derivatives of strain 30-84 that produced only PCA or overproduced 2-OH-PCA. Using flow cell assays, we found that these PZ-altered derivatives of strain 30-84 differed from the wild type in initial attachment, mature biofilm architecture, and dispersal from biofilms. For example, increased 2-OH-PCA production promoted initial attachment and altered the three-dimensional structure of the mature biofilm relative to the wild type. Additionally, both alterations promoted thicker biofilm development and lowered dispersal rates compared to the wild type. The PZ-altered derivatives of strain 30-84 also differed in their ability to inhibit the fungal pathogen G. graminis var. tritici. Loss of 2-OH-PCA resulted in a significant reduction in the inhibition of G. graminis var. tritici. Our findings suggest that alterations in the ratios of antibiotic secondary metabolites synthesized by an organism may have complex and wide-ranging effects on its biology.

scholarly journals Inhibitory effect of a natural phenolic compound, 3-p-trans-coumaroyl-2-hydroxyquinic acid against the attachment phase of biofilm formation of Staphylococcus aureus through targeting sortase A

RSC Advances ◽  
2019 ◽  
Vol 9 (56) ◽  
pp. 32453-32461
Author(s):  
Yan-Ping Wu ◽  
Xiao-Yan Liu ◽  
Jin-Rong Bai ◽  
Hong-Chen Xie ◽  
Si-Liang Ye ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Phenolic Compound ◽  
Biofilm Formation ◽  
Inhibitory Effect ◽  
Sortase A ◽  
Initial Attachment ◽  
Biofilm Development

3-p-trans-Coumaroyl-2-hydroxyquinic acid (CHQA), a natural phenolic compound, prevented Staphylococcus aureus biofilm formation due to the inhibition of the initial attachment stage of biofilm development by targeting sortase A.

Interspecies Interactions of Metal-Oxidizing Thermo-Acidophilic Archaea Acidianus and Sulfolobus

2015 ◽  
Vol 1130 ◽  
pp. 105-108 ◽  
Author(s):  
Rui Yong Zhang ◽  
Jing Liu ◽  
Thomas R. Neu ◽  
Qian Li ◽  
Sören Bellenberg ◽  
...  
Keyword(s):  
Biofilm Formation ◽  
Laser Scanning ◽  
Fluorescent Dyes ◽  
Mine Drainage ◽  
Cell Attachment ◽  
Thermophilic Bacteria ◽  
Laser Scanning Microscopy ◽  
Physical Contact ◽  
Confocal Laser ◽  
Acidophilic Archaea

Biofilm formation of microorganisms on relevant surfaces is of great importance for biomining and acid mine drainage (AMD). Thermo-acidophilic archaea like Acidianus, Sulfolobus and Metallosphaera are of special interest due to their ability to enhance leaching rates. Visualization and investigation of microbial attachment and biofilm formation of metal-oxidizing organisms up to now has been done mostly with mesophilic or moderately thermophilic bacteria. In this study, attachment and biofilms by the crenarchaeota Sulfolobus metallicus DSM 6482T and a new isolate Acidianus sp. DSM 29099 on sulfur or pyrite were analyzed. Confocal laser scanning microscopy (CLSM) combined with fluorescent dyes specific for nucleic acids or glycoconjugates were used to monitor biofilm formation on surfaces. The data indicate that cell attachment and the subsequently formed biofilm structures were species and substrate dependent. The investigation of binary biofilms on pyrite showed that both species were heterogeneously distributed on pyrite surfaces in the form of individual cells or microcolonies. In addition, physical contact between the two species was visible, as revealed by specific lectins able to distinguish single species.

scholarly journals Umbelliferone Impedes Biofilm Formation and Virulence of Methicillin-Resistant Staphylococcus epidermidis via Impairment of Initial Attachment and Intercellular Adhesion

2019 ◽  
Vol 9 ◽  
Author(s):  
Thirukannamangai Krishnan Swetha ◽  
Murugesan Pooranachithra ◽  
Ganapathy Ashwinkumar Subramenium ◽  
Velayutham Divya ◽  
Krishnaswamy Balamurugan ◽  
...  
Keyword(s):  
Staphylococcus Epidermidis ◽  
Biofilm Formation ◽  
Intercellular Adhesion ◽  
Initial Attachment ◽  
Methicillin Resistant

scholarly journals Absence of TolC Impairs Biofilm Formation in Actinobacillus pleuropneumoniae by Reducing Initial Attachment

PLoS ONE ◽  
2016 ◽  
Vol 11 (9) ◽  
pp. e0163364 ◽  
Author(s):  
Ying Li ◽  
Sanjie Cao ◽  
Luhua Zhang ◽  
Jianlin Yuan ◽  
Gee W. Lau ◽  
...  
Keyword(s):  
Biofilm Formation ◽  
Actinobacillus Pleuropneumoniae ◽  
Initial Attachment

scholarly journals Fluorescence-Based Quasicontinuous andIn SituMonitoring of Biofilm Formation Dynamics in Natural Marine Environments

2014 ◽  
Vol 80 (12) ◽  
pp. 3721-3728 ◽  
Author(s):  
M. Fischer ◽  
G. Friedrichs ◽  
T. Lachnit
Keyword(s):  
Diurnal Variation ◽  
Biofilm Formation ◽  
Bacterial Attachment ◽  
Smooth Transition ◽  
Marine Environments ◽  
Initial Attachment ◽  
Eukaryotic Microorganisms ◽  
Formation Dynamics ◽  
Adaptation Phase

ABSTRACTAnalyzing the dynamics of biofilm formation helps to deepen our understanding of surface colonization in natural environments. While methods for screening biofilm formation in the laboratory are well established, studies in marine environments have so far been based upon destructive analysis of individual samples and provide only discontinuous snapshots of biofilm establishment. In order to explore the development of biofilm over time and under various biotic and abiotic conditions, we applied a recently developed optical biofilm sensor to quasicontinuously analyze marine biofilm dynamicsin situ. Using this technique in combination with microscope-assisted imaging, we investigated biofilm formation from its beginning to mature multispecies biofilms. In contrast to laboratory studies on biofilm formation, a smooth transition from initial attachment to colony formation and exponential growth could not be observed in the marine environment. Instead, initial attachment was followed by an adaptation phase of low growth and homogeneously distributed solitary bacterial cells. Moreover, we observed a diurnal variation of biofilm signal intensity, suggesting a transient state of biofilm formation of bacteria. Overall, the biofilm formation dynamics could be modeled by three consecutive development stages attributed to initial bacterial attachment, bacterial growth, and attachment and growth of unicellular eukaryotic microorganisms. Additional experiments showed that the presence of seaweed considerably shortened the adaptation phase in comparison with that on control surfaces but yielded similar growth rates. The outlined examples highlight the advantages of a quasicontinuousin situdetection that enabled, for the first time, the exploration of the initial attachment phase and the diurnal variation during biofilm formation in natural ecosystems.

scholarly journals Curli fimbriae are conditionally required in Escherichia coli O157:H7 for initial attachment and biofilm formation

2016 ◽  
Vol 57 ◽  
pp. 81-89 ◽  
Author(s):  
Michelle Qiu Carter ◽  
Jacqueline W. Louie ◽  
Doris Feng ◽  
Wayne Zhong ◽  
Maria T. Brandl
Keyword(s):  
Escherichia Coli ◽  
Biofilm Formation ◽  
Escherichia Coli O157 ◽  
Initial Attachment
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