Study on One-Step Solid-State Fermentation of Soybean Meal

2011 ◽  
Vol 236-238 ◽  
pp. 2836-2839
Author(s):  
Tao Xiong ◽  
Jian Fei Liu ◽  
Qian Qian Guan ◽  
Su Hua Song
Keyword(s):  
Solid State ◽  
Solid State Fermentation ◽  
Anaerobic Fermentation ◽  
Orthogonal Experiment ◽  
Inoculum Size ◽  
Fermentation Temperature ◽  
Before And After ◽  
One Step ◽  
Fermentation Period ◽  
Water Ratio

one-step solid state fermentation process was studied. The orthogonal experiment was carried out to study the effect of the inoculation ratio, the inoculum size, the fermentation water ratio, the fermentation temperature and the fermentation period in this experiment. Optimum conditions were as follows: Bacillus licheniformis: yeast: Lactobacillus plantarum = 2:1:1, the inoculation was 6.0g/100g, the water ratio was 1:0.8, the anaerobic fermentation temperature was 36°C, the fermentation period was 96h. The content of trypsin inhibitor was measured and analyzed before and after the fermentation of soybean under the conditions.

A Study on Dry Anaerobic Fermentation Technology of Pineapple Leaf Residue

2011 ◽  
Vol 236-238 ◽  
pp. 178-182
Author(s):  
Yi Guo Deng ◽  
Jin Li Wang ◽  
Jing Jiao ◽  
Yong Zheng ◽  
Gang Wang ◽  
...  
Keyword(s):  
Anaerobic Fermentation ◽  
Biogas Production ◽  
Orthogonal Experiment ◽  
Gas Production ◽  
Inoculum Size ◽  
Solid Content ◽  
Raw Material ◽  
Biogas Yield ◽  
Fermentation Temperature ◽  
Leaf Residue

A self-designed constant temperature fermenter was manufactured and used for this study. Dry anaerobic fermentation experiments were conducted with sugarcane leaf residue as raw material. With the C/N ratio being 25:1, various total solids concentrations (TS), inoculum sizes and fermentation temperatures were selected to study biogas production characteristics. The experiment results showed that biogas yield increased rapidly during the initial stage of reaction, decreased quickly after reaching the peak, and the decrease slowed down at some level. Orthogonal experiment results showed that both fermentation temperature and solids concentration showed significant effects on gas production yield. Fermentation temperature showed the most significant effect, while the effect of inoculum size was not significant on gas yield. The optimum fermentation performance was obtained at 20% solid content, 35°C fermentation temperature, and 30% inoculum size.

scholarly journals Cow Dung Substrate for the Potential Production of Alkaline Proteases by Pseudomonas putida Strain AT in Solid-State Fermentation

2014 ◽  
Vol 2014 ◽  
pp. 1-7 ◽  
Author(s):  
Ponnuswamy Vijayaraghavan ◽  
Sreekumar Saranya ◽  
Samuel Gnana Prakash Vincent
Keyword(s):  
Solid State ◽  
Pseudomonas Putida ◽  
Solid State Fermentation ◽  
Maximum Activity ◽  
Protease Production ◽  
Cow Dung ◽  
Alkaline Proteases ◽  
Optimum Conditions ◽  
Potential Production ◽  
Fermentation Period

Cow dung and agroresidues were used as the substrates for the production of alkaline proteases by Pseudomonas putida strain AT in solid-state fermentation. Among the various substrates evaluated, cow dung supported maximum (1351±217 U/g) protease production. The optimum conditions for the production of alkaline proteases were a fermentation period of 48 h, 120% (v/w) moisture, pH 9, and the addition of 6% (v/w) inoculum, 1.5% (w/w) trehalose, and 2.0% (w/w) yeast extract to the cow dung substrate. The enzyme was active over a range of temperatures (50–70°C) and pHs (8–10), with maximum activity at 60°C and pH 9. These enzymes showed stability towards surfactants, detergents, and solvent and digested various natural proteins.

scholarly journals Optimization of ligninolytic enzymes production from Aspergillus terreus SG-777 by solid state fermentation

2016 ◽  
Vol 40 (1) ◽  
pp. 116-124 ◽  
Author(s):  
Sahar Ghazi Imran
Keyword(s):  
Solid State ◽  
Solid State Fermentation ◽  
Aspergillus Terreus ◽  
Production Condition ◽  
C:N Ratio ◽  
Ligninolytic Enzymes ◽  
Inoculum Size ◽  
Physical Parameters ◽  
Fungal Culture ◽  
Lignocellulosic Substrate

        The present study aimed at producing the ligninolytic enzymes extracts by growing single and co-cultures of an indigenous Aspergillus terreus SG-777 utilizing solid state fermentation (SSF) using lignocellulosic substrates. A further goal was to optimize the production condition of ligninolytic enzymes by selected fungal culture and lignocellulosic substrate. The production process was further improved by optimizing a number of physical parameters such as (substrate, incubation time, moisture level, inoculum size, pH, and temperature). By optimization of different parameters, the maximum specific activities of enzymes synthesized by Aspergillus terreus SG-777 were observed as 0.83 U/mg for manganese peroxidase (MnP), 18.03 U/mg for lignin peroxidase (LiP) and 0.91 U/mg for laccase,  when using the banana stalks as substrate after 8 days incubation at рH 5.5 and 35°C temperature with 1×105 spore/ml ml inoculum size, 1:5 w/v moisture content, 20:1 C:N ratio (glucose and ammonium tartarate as carbon and nitrogen supplements), 1ml of 1mM MnSO4 as mediator, and 1ml of 1mM MgSO4.7H2O2.

scholarly journals Enhancement of Laccase Production by Optimizing the Cultural Conditions for Pleurotus Sajor-Caju in Solid-State Fermentation

2021 ◽  
Author(s):  
Shradhdha Sharma ◽  
Duggirala Srinivas Murty
Keyword(s):  
Solid State ◽  
Moisture Content ◽  
Wheat Straw ◽  
Solid State Fermentation ◽  
Cost Effective ◽  
Inoculum Size ◽  
Laccase Production ◽  
Native Page ◽  
Industrial Sectors ◽  
Pleurotus Sajor Caju

Nowadays, a lot of interest has been given to the development of cost-effective and efficient enzyme production technologies. Laccase enzymes are widely used in biotechnological, environmental and industrial sectors. Due to the cost-effectiveness of the solid-state fermentation (SSF) process, it is widely used to produce a broad range of biological products. In this study, optimization of moisture content, temperature, pH, and inoculum size were studied to enhance laccase production ability of Pleurotus sajor-caju in SSF by using One Factor At Time (OFAT) and Response Surface Methodology (RSM). OFAT was used as a baseline study for deducing the experimental design of RSM. The highest production of laccase enzyme (1450 U/g) by Pleurotus sajor-caju on wheat straw was observed at 26°C, 6.0 pH, 72.5 % moisture content, 7.5% inoculum size, 1% fructose and 0.5 % peptone. Unlike the conventional inoculum preparation method, here the inoculum was generated by the spawning method for SSF. The molecular weight of partially purified laccase from Pleurotus sajor-caju was estimated to be around 62 K Da using SDS PAGE. The activity staining of laccase was observed as a zymogram on Native PAGE using ABTS as a substrate. Lignin degradation of wheat straw and its structural disruption due to laccase was observed by Scanning Electron Microscopy (SEM).

scholarly journals Response surface methodology to optimize inulinase production by a newly isolated Penicillium amphipolaria strain by solid-state fermentation of Saccharum arundinaceum

2020 ◽  
Author(s):  
Deblina Das ◽  
Ramananda M Bhat ◽  
Raja Selvaraj
Keyword(s):  
Response Surface Methodology ◽  
Solid State ◽  
Thin Layer ◽  
Solid State Fermentation ◽  
Low Cost ◽  
Fold Increase ◽  
Media Optimization ◽  
Fermentation Period ◽  
Layer Chromatography ◽  
Central Composite

Abstract In the present investigation, a new fungal inulinase producer Penicillium amphipolaria KAS 2555 has been isolated from the soil of dead mangroves litter area, followed by the inulinase production and optimization by solid-state fermentation using a low-cost substrate – hardy sugarcane (Saccharum arundinaceum). While screening, only Penicillium amphipolaria KAS 2555 showed the hydrolysis zone on the plate containing inulin media. The exoinulolytic nature of inulinase and its form of action was confirmed by thin-layer chromatography (TLC). After 96 h of the fermentation period, an activity of 2.45 U/gds was obtained. The I/S ratio of 0.59 proved that the enzyme is inulolytic in nature. Media optimization was performed to obtain a regression model using Central Composite Design (CCD). For optimization, five significant media components viz., inulin, (NH4)2SO4, K2HPO4, KH2PO4 and, NaCl were used. A 3.10-fold increase in activity of inulinase (7.59 U/gds) was obtained under the optimal settings of (g/gds) inulin- 0.1, (NH4)2SO4- 0.002, K2HPO4- 0.1, KH2PO4- 0.02 and NaCl- 0.02.

scholarly journals Biological Pretreatment by Solid-State Fermentation of Oat Straw to Enhance Physical Quality of Pellets

2020 ◽  
Vol 2020 ◽  
pp. 1-13
Author(s):  
Wei Gao ◽  
Zhiqiang Lei ◽  
Lope G Tabil ◽  
Rongfei Zhao
Keyword(s):  
Particle Size ◽  
Solid State ◽  
Moisture Content ◽  
Solid State Fermentation ◽  
Biological Pretreatment ◽  
Fermentation Time ◽  
Physical Quality ◽  
Fermentation Temperature ◽  
Oat Straw

Pelleting can increase the efficiency of handling and transportation of biomass. Pretreatment obtains lignin fragments by disrupting the lignocellulosic structure of biomass and ensures the high-quality compressed pellets. In this study, solid-state fermentation (SSF) is used as a biological method to improve the quality of pellets of oat straw. SSF of oat straw using Trametes versicolor 52J (TV52J) and Phanerochaete chrysosporium (PC) was conducted. Response surface methodology (RSM) was employed by using a four-factor, three-level Box–Behnken design with fermentation time (days), moisture content (%), particle size (mm), and fermentation temperature (°C) as independent parameters. Pellet density, dimensional stability, and tensile strength were the response variables. The optimization options of fermentation time (33.96 and 35 days), moisture content (70%), particle size (150 and 50 mm), and fermentation temperature (22°C) of oat straw pretreated with these two fungal strains were obtained. The microscopic structural changes of oat straw caused by biological pretreatment were investigated by scanning electron microscopy (SEM). Observation results of SEM showed that the connection between single fibers became relatively loose, and this was beneficial to improve the physical quality of the pellets.

Pectinase Production from Banana Peel Biomass via the Optimization of the Solid-state Fermentation Conditions of Aspergillus niger Strain

2021 ◽  
Vol 30 (1) ◽  
pp. 257-275
Author(s):  
Nazaitulshila Rasit ◽  
Yong Sin Sze ◽  
Mohd Ali Hassan ◽  
Ooi Chee Kuan ◽  
Sofiah Hamzah ◽  
...  
Keyword(s):  
Solid State ◽  
Moisture Content ◽  
Solid State Fermentation ◽  
Incubation Temperature ◽  
Inoculum Size ◽  
Operating Conditions ◽  
Banana Peel ◽  
Fermentation Conditions ◽  
Pectinase Production ◽  
Full Factorial

In this study, the biomass of banana peel was used to produce pectinase via optimization of solid-state fermentation conditions of the filamentous fungi Aspergillus nigeA. niger). The operating conditions of solid-state fermentation were optimized using the method of full factorial design with incubation temperature ranging between 25 °C and 35 °C, moisture content between 40% and 60%, and inoculum size between 1.6 x 106 spores/mL and 1.4 x 107 spores/mL. Optimizing the solid-state fermentation conditions appeared crucial to minimize the sample used in this experimental design and determine the significant correlation between the operating conditions. A relatively high maximal pectinase production of 27 UmL-1 was attained at 35° C of incubation, 60% of moisture content, and 1.6 x 106 spores/mL of inoculum size with a relatively low amount of substrate (5 g). Given that the production of pectinase with other substrates (e.g., pineapple waste, lemon peel, cassava waste, and wheat bran) generally ranges between 3 U/mL and 16 U/mL (Abdullah et al., 2018; Handa et al., 2016; Melnichuk et al., 2020; Thangaratham and Manimegalai, 2014; Salim et al., 2017), thus the yield of pectinase derived from the banana peel in this study (27 U/mL) was considered moderately high. The findings of this study indicated that the biomass of banana peel would be a potential substrate for pectinase production via the solid-state fermentation of A. niger.

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