Study on the Influence Factors of Desilication in Bioleaching by B. Mucilaginosus

2011 ◽  
Vol 415-417 ◽  
pp. 1740-1743
Author(s):  
Xiao Xi Zeng ◽  
Hong Bo Zhou ◽  
Yue Hua Hu ◽  
Jian Xin Tang ◽  
Pei Jiang ◽  
...  

Bioleaching is an environmental friendly technology with many potential advantages such as few pollution and low energy requirement. The factors which affected the silicate-dissolving ability of Bacillus mucilaginosus Lv1-2on illite were investigated. The results showed that the optimum inoculum amount was 2%.The best carbon source and nitrogen source were glucose and (NH4)2SO4respectively. The silicon dissolution increased with the powder diameter decreased. The optimal temperature and shaking speed were 30°C and 220 rpm. The proper conditions were consistent with that of the growth of the strain,which confirmed that the strain played an important role in bioleaching.

2013 ◽  
Vol 709 ◽  
pp. 810-813 ◽  
Author(s):  
Xiong Ya ◽  
Min Jie Li

Termitomyces albuminosus is a kind of local distinctive wild edible fungi in southwest of China. It is delicious, rich in nutrition and has high development and utilization value, but owing to the restrictions of growth environment, it can not be cultivated artificially. This article mainly studied on the biological characteristics of Termitomyces albuminosus Hypha, and found out the optimal carbon source, nitrogen source, growth factors and the C/N ratio that are suitable for the growth of Hypha of Termitomyces albuminosus .


2010 ◽  
Vol 76 (12) ◽  
pp. 4102-4104 ◽  
Author(s):  
Yin Chen ◽  
Kathryn L. McAleer ◽  
J. Colin Murrell

ABSTRACT Monomethylamine can be used by nonmethylotrophs as a sole nitrogen source but not as a carbon source; however, little is known about the genes and enzymes involved. The γ-glutamylmethylamide/N-methylglutamate pathway for monomethylamine utilization by methylotrophs has recently been resolved. We have identified genes encoding key enzymes of this pathway in nonmethylotrophs (e.g., Agrobacterium tumefaciens) and demonstrated that this pathway is also involved in the utilization of monomethylamine as a nitrogen source by nonmethylotrophs.


1975 ◽  
Vol 25 (2) ◽  
pp. 119-135 ◽  
Author(s):  
Meryl Polkinghorne ◽  
M. J. Hynes

SUMMARYWild-type strains ofAspergillus nidulansgrow poorly onL-histidine as a sole nitrogen source. The synthesis of the enzyme histidase (EC. 4.3.1.3) appears to be a limiting factor in the growth of the wild type, as strains carrying the mutantareA102 allele have elevated histidase levels and grow strongly on histidine as a sole nitrogen source.L-Histidine is an extremely weak sole carbon source for all strains.Ammonium repression has an important role in the regulation of histidase synthesis and the relief of ammonium repression is dependent on the availability of a good carbon source. The level of histidase synthesis does not respond to the addition of exogenous substrate.Mutants carrying lesions in thesarA orsarB loci (suppressor ofareA102) have been isolated. The growth properties of these mutants on histidine as a sole nitrogen source correlate with the levels of histidase synthesized. Mutation at thesarA andsarB loci also reduces the utilization of a number of other nitrogen sources. The data suggest that these two genes may code for regulatory products involved in nitrogen catabolism. No histidase structural gene mutants were identified and possible explanations of this are discussed.


2018 ◽  
Vol 78 (10) ◽  
pp. 2204-2213 ◽  
Author(s):  
Liang Cheng ◽  
Raphael Marie-Guillaume Flavigny ◽  
Md Iqbal Hossain ◽  
Wipa Charles ◽  
Ralf Cord-Ruwisch

Abstract The current paper describes a novel passive aeration simultaneous nitrification and denitrification (PASND) zeolite amended biofilm reactor that removes organic carbon and nitrogen from wastewater with low-energy consumption. Next to the ammonium oxidizing bacteria (AOB), this reactor contained naturally enriched glycogen accumulating organisms (GAOs) and zeolite powder to initially adsorb BOD (acetate) and ammonium (NH4+-N) from synthetic wastewater under anaerobic conditions. Draining of the treated wastewater exposed the biofilm directly to air enabling low-energy oxygen supply by passive aeration. This allowed the adsorbed ammonium to be oxidized by the AOB and the produced nitrite and nitrate to be reduced simultaneously by the GAOs using the adsorbed BOD (stored as PHAs) as carbon source. Overall, with an operation mode of 1 h anaerobic and 4 h aerobic phase, the nutrient removal efficiency after single treatment was about 94.3% for BOD and 72.2% for nitrogen (NH4+-N). As high-energy aeration of the bulk solution for oxygen supply is completely avoided, the energy requirement of the proposed PASND biofilm reactor can be theoretically cut down to more than 50% compared to the traditional activated sludge process.


2011 ◽  
Vol 2011 ◽  
pp. 1-4 ◽  
Author(s):  
Shiyi Ou ◽  
Jing Zhang ◽  
Yong Wang ◽  
Ning Zhang

A mixture of wheat bran with maize bran as a carbon source and addition of (NH4)SO4 as nitrogen source was found to significantly increase production of feruloyl esterase (FAE) enzyme compared with wheat bran as a sole carbon and nitrogen source. The optimal conditions in conical flasks were carbon source (30 g) to water 1 : 1, maize bran to wheat bran 1 : 2, (NH4)SO4 1.2 g and MgSO4 70 mg. Under these conditions, FAE activity was 7.68 mU/g. The FAE activity on the mixed carbon sources showed, high activity against the plant cell walls contained in the cultures.


2014 ◽  
Vol 5 (2) ◽  
Author(s):  
Ermina Sari

SRC-4 starin is one of bacteria sellulose producing strains. It was obtained from the rottencoconut meat from Semplak, Bogor. The objective of this study is to obtain the optimumcomposition of modified HS medium to produce cellulose, by increasing yeast extractconcentration and also combination of carbon and vitamin sources. The production of celluloseby SRC-4 was done by 18 treatments on static culture. The increasing of yeast extractconcentration, combination of carbon and vitamin sources gave significant effect to theproduction of cellulose. The highest yield of cellulose was produced at the treatment consist of1.0 % yeat extract (as nitrogen source), 1 % glucose + 1 % glycerol (as carbon source) and0.164 ppm vitamin B1 + 0.020 ppm vitamin B2 (as vitamin source).


2020 ◽  
Vol 31 (1) ◽  
pp. 15
Author(s):  
Dr.Neihaya Heikmat Zaki

Twenty five samples were collected from the soil around the Tigris River from different locations in Iraqi cities, and 45 bacterial isolates were obtained. Three of these isolates were further tested for their degrading capacity of Bisphenol A (BPA) in Basal Mineral Medium, included: Pseudomonas orizohibtanis, Escherishia coli and Proteus penneri. The optimal temperature for the removal of BPA was determined at 20˚C, 37˚ and 45˚C for 1, 5, and 15 days, and the degradation increased up to a temperature of 37°C. Growth test was performed on isolated bacteria with BisPhenol A as the sole carbon source, and with increasing incubation time, the culture grew almost linearly to 24 hours. BPA decreased after 1days after incubating with tested bacterial isolates, and almost broken after 5 days, while it disappeared after 15 days at 37C, and Pseudomonas orizohibtanis exhibited the best degradation of BPA. The absorbance peaks in the UV region appeared at 222 and 276 nm and attributed to the benzene ring and triazine ring respectively. The end products of BPA degradation were analyzed by GCMS after 15 days of incubation. The chromatogram for Pseudomanas orizohibtanis showed three peaks at retention times of 70, 210 and 280 min, and referred to hexasiloxane, heptasiloxane, and Octasiloxane respectively. The present study was aimed to isolate bacteria from the soil of the Tigris River, and determined the ability to degrade Bisphenol-A, and characterized the environmental conditions of bacterial growth, and then analysis the products of the degradation by GC-MS.


2018 ◽  
Vol 42 (17) ◽  
pp. 14332-14339 ◽  
Author(s):  
Nan Zhou ◽  
Xingwei Zhang ◽  
Yanping Shi ◽  
Zeliang Li ◽  
Zhibiao Feng

Nitrogen-doped carbon quantum dots (CDs) were synthesized in ethanol media by using citric acid (CA) as the carbon source and ethanediamine (EDA) as the nitrogen source.


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