Producing Cellulase on Phanerochaete chrysosporium by Fermentation and Breeding by Induced Mutation of Cellulose-Degrading Phanerochaete chrysosporium

2012 ◽  
Vol 535-537 ◽  
pp. 2353-2356
Author(s):  
Ying Wang ◽  
Jong Shik Chung ◽  
Guang Li Cao ◽  
Lei Zhao ◽  
Ai Jie Wang
Keyword(s):  
Carbon Source ◽  
Nitrogen Source ◽  
Phanerochaete Chrysosporium ◽  
Filter Paper ◽  
Carboxymethyl Cellulose ◽  
Fermentation Process ◽  
Corn Stalk ◽  
Optimal Conditions ◽  
Ultraviolet Ray ◽  
Filter Paper Activity

In this work, strain Phanerochaete chrysosporium producing cellulase was studied by fermentation process. Carbon source involves cellobiose, xylose, xylan, carboxymethylcellulose sodium, avicel, filter paper, corn stalk and glucose. Peptone, yeastextract, carbamide, ammonium nitrate, ammonium sulfate were researched for nitrogen source. Under the conditions of carbon source avicel, nitrogen source ammonium, the activity assays on different enzymes of respective component is optimum. Ultraviolet ray and nitrosoguanidine,were applied to mutate Phanerochaete chrysosporium. The optimal conditions were as follow: 60S and 50min. Colony morphology with obvious variation was used as sceening index. 120mutants were selected, carboxymethyl cellulose activity of cach mutant was determined and 20 strains with 30% increasing of cellulose activity were screened.GD3 mutant was obtained, with the increasing rate of filter paper activity 30.50%.

The Mixed Bacteria Trichoderma Optimized Conditions

2011 ◽  
Vol 183-185 ◽  
pp. 753-756
Author(s):  
Song Han ◽  
Guo Qiang Li ◽  
Si Yao Guo ◽  
Jin Bing Sun ◽  
Feng Lu Wang ◽  
...  
Keyword(s):  
Enzyme Activity ◽  
Filter Paper ◽  
Carboxymethyl Cellulose ◽  
Trichoderma Viride ◽  
Inoculum Size ◽  
Optimal Conditions ◽  
Mixed Bacteria ◽  
Optimized Conditions ◽  
Effective Conditions ◽  
Filter Paper Activity

In this paper, we researched the activity of the trichoderma viride and trichoderma koningi, and measure the enzyme activity of carboxymethyl cellulose (CMC) and filter paper activity (FPA) of them. And then, mixed trichoderma viride and trichoderma koningi to determine the optimal conditions, which according to influence of the revolutions, the layer of gauze, temperature. It turned out that the effective conditions was: 150r/min of revolutions, 3 layers of gauze, two wafer with 2 cm in diam of inoculum size and 30°C.

scholarly journals Production of Feruloyl Esterase from Aspergillus niger by Solid-State Fermentation on Different Carbon Sources

2011 ◽  
Vol 2011 ◽  
pp. 1-4 ◽  
Author(s):  
Shiyi Ou ◽  
Jing Zhang ◽  
Yong Wang ◽  
Ning Zhang
Keyword(s):  
Carbon Source ◽  
Nitrogen Source ◽  
Wheat Bran ◽  
Cell Walls ◽  
Carbon Sources ◽  
Feruloyl Esterase ◽  
Plant Cell Walls ◽  
Optimal Conditions ◽  
Carbon And Nitrogen ◽  
Maize Bran

A mixture of wheat bran with maize bran as a carbon source and addition of (NH4)SO4 as nitrogen source was found to significantly increase production of feruloyl esterase (FAE) enzyme compared with wheat bran as a sole carbon and nitrogen source. The optimal conditions in conical flasks were carbon source (30 g) to water 1 : 1, maize bran to wheat bran 1 : 2, (NH4)SO4 1.2 g and MgSO4 70 mg. Under these conditions, FAE activity was 7.68 mU/g. The FAE activity on the mixed carbon sources showed, high activity against the plant cell walls contained in the cultures.

scholarly journals Optimization of nitrogen source for Bifidobacterium bifidum using response surface methodology

2016 ◽  
Vol 20 (1) ◽  
pp. 53-64 ◽  
Author(s):  
He Chen ◽  
Jianbo Kou ◽  
Man Hu ◽  
Guowei Shu
Keyword(s):  
Response Surface Methodology ◽  
Carbon Source ◽  
Central Composite Design ◽  
Liquid Medium ◽  
Nitrogen Source ◽  
Bifidobacterium Bifidum ◽  
Optimal Conditions ◽  
Medium Carbon ◽  
Central Composite ◽  
Mrs Medium

Abstract In order to improve the viable counts of Bifidobacterium bifidum BB01 in the liquid medium, the Central Composite Design (CCD) was used to optimize the nitrogen source in the medium of B. bifidum BB01. The results showed that the nitrogen source composition of B. bifidum BB01 was: peptone 0.9%, yeast extracts 0.3%, beef paste 0.7%. Under the optimal conditions, the viable counts of B. bifidum BB01 reached (2.49±0.06)×109CFU/mL after cultured at 18h, which was 42.97% higher than MRS (lactose), and 12.85% higher than the optimized MRS medium (carbon source and prebiotics were optimized). Therefore, the CCD used in this study is workable for promoting the growth of B. bifidum BB01.

scholarly journals Molecular, physiological, and biochemical characterization of extracellular lipase production by Aspergillus niger using submerged fermentation

PeerJ ◽  
2020 ◽  
Vol 8 ◽  
pp. e9425
Author(s):  
Amira Hassan Alabdalall ◽  
Norah Ayad ALanazi ◽  
Sumayh A. Aldakeel ◽  
Sayed AbdulAzeez ◽  
J. Francis Borgio
Keyword(s):  
Genetic Diversity ◽  
Carbon Source ◽  
Nitrogen Source ◽  
Lipase Activity ◽  
Submerged Fermentation ◽  
Lipase Production ◽  
Group Method ◽  
Phenol Red ◽  
Optimal Conditions

Background Extracellular production of fungal lipases especially the lipases obtained from the Aspergilli has gained immense interest in recent years due to its diverse biotechnological applications. In this study, we focused on determining the fermentation parameters required for the optimal lipase production. Methods A total of 256 fungal isolates were obtained from oil seeds. From each genus, one isolate was selected to evaluate lipase production using phenol red and tributyrin plate assays. Lipase activity was estimated using the spectrophotometric pNPP hydrolysis assay. The highest lipase producer isolates were identified using 18S ribosomal RNA gene sequencing. The genetic variability was determined by random amplified polymorphic DNA (RAPD) analysis and the dendrogram was constructed using the unweighted pair group method with arithmetic averages method. The isolates were examined in a submerged fermentation culture (Smf) to measure the effect of temperature, pH, incubation time, carbon source, nitrogen source, inoculum volume, and lipid source on lipase production. Results Eleven isolates belonging to the genus Aspergillus were analyzed for lipase production where they were found to be the highest lipase producers among various fungal genera. All the tested isolates were identified as A. niger using 18s rRNA sequencing. Genetic diversity was evaluated among all of the studied A. niger isolates using RAPD primers. The RAPD primers were used to amplify 285 loci, of which five were polymorphic (1.75%) and seven were monomorphic (2.45%). Thus, a high level of genetic diversity was observed among all isolates. The tributyrin test and the lipase activity assay identified five strains of A. niger as high lipase producers, and their optimal enzyme activities were 709.74, 532.54, 735.64, 794.62, and 787.69 U/ml. The optimal conditions for lipase production were as follows: 40 °C, pH 7.5, 1% fructose as the carbon source, 1% yeast extract as the nitrogen source, 2% palm oil, 2.5 × 107 spores/ml suspension, and 3 days of incubation. Conclusions The current study provides a comprehensive characterization of the optimal conditions, which are essential to enhance lipase production in five A. niger isolates.

scholarly journals ISOLASI BAKTERI SELULOLITIK PENDEGRADASI SELULOSA DARI KOMPOS

2019 ◽  
Vol 7 (1) ◽  
pp. 30
Author(s):  
Zainul Arifin ◽  
Ida Bagus Wayan Gunam ◽  
Nyoman Semadi Antara ◽  
Yohanes Setiyo
Keyword(s):  
Carbon Source ◽  
Filter Paper ◽  
Congo Red ◽  
Carboxymethyl Cellulose ◽  
Cellulolytic Activity ◽  
Cellulolytic Bacteria ◽  
Clear Zone ◽  
Degradation Test ◽  
Whatman Paper ◽  
Congo Red Test

This study aimed to isolate cellulolytic bacteria that have the potential to degrade cellulose taken compost samples from Temesi and Bondowoso, this study was also conducted to determine the ability of bacteria to degrade cellulose based on Congo red test and filter paper degradation test. All isolates were tested for cellulolytic activity using soluble Carboxymethyl Cellulose (CMC). Characterization was carried out by growing selected pure isolates on CMC media and then dripping 0.1% congo red to test the cellulolytic potential (cellulolytic potential was characterized by the emergence of clear zones around the colony). The results of isolation of bacteria obtained 38 isolates, namely 26 Bondowoso sample isolates and 12 Temesi isolates that were able to grow and utilize cellulose as a carbon source. But only fourteen isolates produced clear zones in the Congo red test with diameters ranging from 1.66  cm to 6.76 cm. Six isolates that have the largest diameters clear zone, were tested for  degradation of filter paper  (Whatman no. 1). Isolates bacteria of B2S8 obtained from Bondowoso compost samples has the highest ability to degrade cellulose on Whatman paper no. 1 as much as 51.30%. Keywords: Compost, Isolation, Screening, Cellulolytic Bacteria.

Studies on the Biological Characteristics of Termitomyces Albuminosus Hypha

2013 ◽  
Vol 709 ◽  
pp. 810-813 ◽  
Author(s):  
Xiong Ya ◽  
Min Jie Li
Keyword(s):  
Growth Factors ◽  
Carbon Source ◽  
Nitrogen Source ◽  
Biological Characteristics ◽  
Edible Fungi ◽  
Growth Environment ◽  
Wild Edible Fungi ◽  
Development And Utilization

Termitomyces albuminosus is a kind of local distinctive wild edible fungi in southwest of China. It is delicious, rich in nutrition and has high development and utilization value, but owing to the restrictions of growth environment, it can not be cultivated artificially. This article mainly studied on the biological characteristics of Termitomyces albuminosus Hypha, and found out the optimal carbon source, nitrogen source, growth factors and the C/N ratio that are suitable for the growth of Hypha of Termitomyces albuminosus .

scholarly journals Monomethylamine as a Nitrogen Source for a Nonmethylotrophic Bacterium, Agrobacterium tumefaciens

2010 ◽  
Vol 76 (12) ◽  
pp. 4102-4104 ◽  
Author(s):  
Yin Chen ◽  
Kathryn L. McAleer ◽  
J. Colin Murrell
Keyword(s):  
Agrobacterium Tumefaciens ◽  
Carbon Source ◽  
Nitrogen Source ◽  
Sole Nitrogen Source ◽  
Key Enzymes ◽  
Genes Encoding

ABSTRACT Monomethylamine can be used by nonmethylotrophs as a sole nitrogen source but not as a carbon source; however, little is known about the genes and enzymes involved. The γ-glutamylmethylamide/N-methylglutamate pathway for monomethylamine utilization by methylotrophs has recently been resolved. We have identified genes encoding key enzymes of this pathway in nonmethylotrophs (e.g., Agrobacterium tumefaciens) and demonstrated that this pathway is also involved in the utilization of monomethylamine as a nitrogen source by nonmethylotrophs.

scholarly journals Mutants affecting histidine utilization inAspergillus nidulans

1975 ◽  
Vol 25 (2) ◽  
pp. 119-135 ◽  
Author(s):  
Meryl Polkinghorne ◽  
M. J. Hynes
Keyword(s):  
Carbon Source ◽  
Nitrogen Source ◽  
Sole Carbon Source ◽  
Nitrogen Sources ◽  
Limiting Factor ◽  
Sole Nitrogen Source ◽  
Wild Type ◽  
Exogenous Substrate ◽  
Growth Properties ◽  
Type Strains

SUMMARYWild-type strains ofAspergillus nidulansgrow poorly onL-histidine as a sole nitrogen source. The synthesis of the enzyme histidase (EC. 4.3.1.3) appears to be a limiting factor in the growth of the wild type, as strains carrying the mutantareA102 allele have elevated histidase levels and grow strongly on histidine as a sole nitrogen source.L-Histidine is an extremely weak sole carbon source for all strains.Ammonium repression has an important role in the regulation of histidase synthesis and the relief of ammonium repression is dependent on the availability of a good carbon source. The level of histidase synthesis does not respond to the addition of exogenous substrate.Mutants carrying lesions in thesarA orsarB loci (suppressor ofareA102) have been isolated. The growth properties of these mutants on histidine as a sole nitrogen source correlate with the levels of histidase synthesized. Mutation at thesarA andsarB loci also reduces the utilization of a number of other nitrogen sources. The data suggest that these two genes may code for regulatory products involved in nitrogen catabolism. No histidase structural gene mutants were identified and possible explanations of this are discussed.

OPTIMIZATION OF BIOVANILLIN PRODUCTION OF LEMONGRASS LEAVES HYDROLYSATES THROUGH PHANEROCHAETE CHRYSOSPORIUM

2015 ◽  
Vol 77 (31) ◽  
Author(s):  
Huszalina Hussin ◽  
Madihah Md Salleh ◽  
Chong Chun Siong ◽  
Muhammad Abu Naser ◽  
Suraini Abd- Aziz ◽  
...  
Keyword(s):  
Ferulic Acid ◽  
Nitrogen Source ◽  
Phanerochaete Chrysosporium ◽  
Organic Nitrogen ◽  
Nitrogen Sources ◽  
Optimum Conditions ◽  
Molar Yield ◽  
Primary Screening ◽  
Organic Nitrogen Source ◽  
Inorganic And Organic Nitrogen

The recent study has demonstrated the effects of different nitrogen sources on vanillin production by Phanerochaete chrysosporium. Primary screening supported maximum biotransformation of ferulic acid (from lemongrass leaves hydrolysate) to vanillin by using ammonium chloride and yeast extract as inorganic and organic nitrogen source, respectively. With the 2-level factorial analysis, the optimum conditions of vanillin production from ferulic acid by P. chrysosporium was achieved at 0.192g/L with a molar yield of 24.5%.

Sign in / Sign up

Export Citation Format

Share Document