Four Flavonoid Glycosides from the Pulps of Elaeagnus angustifolia and their Antioxidant Activities

2013 ◽  
Vol 756-759 ◽  
pp. 16-20 ◽  
Author(s):  
Ya Wang ◽  
Tao Guo ◽  
Jia Yin Li ◽  
Shang Zhen Zhou ◽  
Ping Zhao ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Activities ◽  
Reducing Power ◽  
Flavonoid Glycosides ◽  
Dependent Manner ◽  
Elaeagnus Angustifolia ◽  
Superoxide Anions ◽  
Dpph Radical ◽  
First Time ◽  
Dose Dependent

Four flavonoid glycosides were isolated from the pulps of Elaeagnus angustifolia and identified as Quercetin 3,4'-O-β-D-diglucoside (1), Isorhamnetin-3-O-β-D-galactopy-ranoside (2), Quercetin 3-O-β-D-Galactopyranoside-4'-O-β-D-glucopyranoside (3) and Isorhamnetin 3-O-β-D-Galacto-pyranoside-4'-O-β-D-glucopyranoside (4). Among which, the compounds 1, 3 and 4 were isolated for the first time from this plant. The antioxidant activities of the four compounds were evaluated by measuring their reducing power and ability to scavenge the ABTS radical, DPPH radical and to inhibit superoxide anions. The results indicated that theses compounds exhibited antioxidant activities with a dose-dependent manner in all tested models, and had some structureactivity relationships. The order of potential antioxidant capacity was as follows:1>3>2>4. Considering related reports, the flavonoid might be responsible for antioxidant activity of pulps of E. angustifolia.

Antioxidant Properties of Ethanolic Extract from Ramulus mori (Sangzhi)

2009 ◽  
Vol 15 (5) ◽  
pp. 435-444 ◽  
Author(s):  
Zuofa Zhang ◽  
Jie Jin ◽  
Liangen Shi
Keyword(s):  
Antioxidant Activity ◽  
Superoxide Radical ◽  
Antioxidant Activities ◽  
Antioxidant Properties ◽  
Reducing Power ◽  
Ethanolic Extract ◽  
Experimental Models ◽  
Hplc Method ◽  
Total Phenolic ◽  
Dependent Manner

The antioxidant properties and total phenolic contents of four fractions of ethanolic extract from Ramulus mori were examined. Various experimental models including superoxide radical, hydroxyl radical, 1,1-diphenyl-2-picrylhydrazyl radicals (DPPH) scavenging activity, metal chelating activity, and reducing power were used for characterization of their antioxidant activity. The four fractions showed various degrees of efficacy in each assay in a dose-dependent manner. The third fraction with the highest amount of total phenolics was the most potent antioxidant in all assays used. In addition, the most powerful compound (oxyresveratrol) was isolated and identified followed by on-line HPLC method and characterized by different spectral analysis. Oxyresveratrol exhibited impressive antioxidant activities in scavenging the superoxide radical, hydroxide radical, and DPPH. On the basis of the results obtained, Ramulus mori may serve as a potential source of natural antioxidant due to its significant antioxidant activity and oxyresveratrol may be the most powerful antioxidant in ethanolic extracts of Ramulus mori.

Antihemolytic and antioxidant activities of Allium paradoxum

2010 ◽  
Vol 5 (3) ◽  
pp. 338-345 ◽  
Author(s):  
Mohammad Ebrahimzadeh ◽  
Seyed Nabavi ◽  
Seyed Nabavi ◽  
Bahman Eslami
Keyword(s):  
Antioxidant Activity ◽  
Vitamin C ◽  
Aerial Part ◽  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Dependent Manner ◽  
Vitro Assay ◽  
Aerial Parts

AbstractAntioxidant activity of the aerial part and bulbs of Allium paradoxum was investigated by eight in vitro assay systems. Extracts showed good antioxidant activity. IC50 for 1,1-diphenyl-2-picryl hydrazyl radical-scavenging activity was 890.9±43.2 and 984.9±33.5 µg/ml for the aerial part and bulbs, respectively. The aerial parts have better reducing power than bulb extracts but not comparable with Vitamin C (P>0.001). Extracts showed weak Fe2+ chelating ability, the IC50 being 959±47 and 530±24 µg/ml for bulbs and aerial parts, respectively. Both tested extracts exhibited good hydrogen peroxide scavenging in a concentration dependent manner. They exhibited good antioxidant activity against the hemoglobin-induced linoleic acid system that was comparable with vitamin C (P>0.01). They showed good activity against cumene hydro peroxide induced hemolysis in RBCs. In addition, they possessed antihemolytic activity. The extract from aerial parts had significantly higher total phenol and flavonoid content than did bulbs. Amounts of eight elements (Cu, Mn, Zn, Fe, Ni, Pb, Cd and Cr) were also determined in the bulb and aerial part using atomic absorption spectroscopy. They contained higher Fe and Mn contents than other elements.

scholarly journals Canthium parviflorum Leaves: Antioxidant Activity in Food and Biological Systems, pH, and Temperature Stability

2014 ◽  
Vol 2014 ◽  
pp. 1-7 ◽  
Author(s):  
Vanitha Reddy Palvai ◽  
Sowmya Mahalingu ◽  
Asna Urooj
Keyword(s):  
Antioxidant Activity ◽  
Methanol Extract ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Temperature Stability ◽  
Dependent Manner ◽  
Oil Emulsion ◽  
Dose Dependent

Canthium parviflorum leaves were analyzed for their proximate and phytochemical composition. The leaves were extracted with methanol (ME) and analyzed for antioxidant activity by radical scavenging method, reducing power, ferric reducing capacity, and in vitro inhibition of Fenton’s reagent induced oxidation in oil emulsion and microsomes. In addition, the effect of high temperature (100°C, 15 and 30 min) and pH (4.5, 7, and 9) on the antioxidant activity of ME was investigated. The leaves were rich in polyphenols, flavonoids β-carotene, glutathione, α-tocopherol, and ascorbic acid. The ME exhibited varying degree of antioxidant activity in a dose dependent manner. The RSA was 68%–500 μg. Reducing potency was 0.34 and FRAP was 1.377. Canthium exhibited greater inhibition of oxidation in microsomes (73%) than in the oil emulsion (21%). Heat treatment resulted in reduction of radical scavenging activity of extract from 68% to 40%. At pH 4.5 and 7 methanol extract exhibited some percent of antioxidant activity which ranged between 18 and 32%. Data indicates Canthium as a good source of antioxidants and methanol extract exhibited good antioxidant activity.

scholarly journals Pharmacological insights into Merremia vitifolia (Burm.f.) Hallier f. leaf for its antioxidant, thrombolytic, anti-arthritic and anti-nociceptive potential

2020 ◽  
Author(s):  
Sahida Akter ◽  
Israt Jahan ◽  
Riniara Khatun ◽  
Mohammad Forhad Khan ◽  
Laiba Arshad ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Protein Denaturation ◽  
Antioxidant Activities ◽  
Reducing Power ◽  
Clot Lysis ◽  
Dependent Manner ◽  
Aqueous Fraction ◽  
Writhing Test ◽  
Dose Dependent

Merremia vitifolia (Burm.f.) Hallier f., an ethnomedicinally important plant, used in the tribal areas to treat various ailments including fever, headache, eye inflammation, rheumatism, dysentery, jaundice and urinary diseases. The present study explored the biological efficacy of the aqueous fraction of M. vitifolia leaves (AFMV) through in vitro and in vivo experimental models. The thrombolytic and anti-arthritic effects of AFMV were evaluated by using the clot lysis technique and inhibition of protein denaturation technique, respectively. The anti-nociceptive activity of AFMV was investigated in Swiss Albino mice by acetic acid-induced writhing test and formalin-induced paw licking test. The antioxidant activities of AFMV, including 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical and total reducing power, were also tested. The qualitative phytochemical assays exhibited AFMV contains secondary metabolites such as alkaloid, carbohydrate, flavonoid, tannin, triterpenoids and phenols. In addition, AFMV showed strong antioxidant effects with the highest scavenging activity (IC50 146.61 µg/mL) and reducing power was increased with a dose-dependent manner. AFMV also revealed notable clot lysis effect and substantial anti-arthritic activity at higher doses (500 µg/mL) as  compared to the control. The results demonstrated a promising reduction of the number of writhing and duration of paw licking in acetic acid-induced writhing test and formalin-induced paw licking test in a dose-dependent manner, respectively. In conclusion, AFMV provides the scientific basis of its folkloric usage,  suggesting it as the vital source of dietary supplement.

Antioxidant Activity of Methanol Extract of the Lotus Leaf (Nelumbo nucifera Gertn.)

2003 ◽  
Vol 31 (05) ◽  
pp. 687-698 ◽  
Author(s):  
Ming-Jiuan Wu ◽  
Lisu Wang ◽  
Ching-Yi Weng ◽  
Jui-Hung Yen
Keyword(s):  
Antioxidant Activity ◽  
Metal Binding ◽  
Methanol Extract ◽  
Antioxidant Activities ◽  
Reducing Power ◽  
Nelumbo Nucifera ◽  
Vitro Assay ◽  
Lotus Leaf ◽  
Dose Dependent

In this study, hydrogen peroxide ( H 2 O 2)-mediated Caco-2 cytotoxicity was employed to investigate the potential antioxidant activity of the methanol extract from the lotus leaf (Nelumbo nucifera Gertn.). A dose-dependent protective effect against reactive oxygen species (ROS)-induced cytotoxicity was observed when Caco-2 cells were treated with 10 mM H 2 O 2 in combination with the methanol extract of the lotus leaf (0.1–0.3 mg/ml). However, no significant effect was found when co-treating Caco-2 cells with 10 mM H 2 O 2 and α-tocopherol. In vitro assay revealed that the extract exhibited scavenging activities on free radicals and hydroxyl radicals, and metal binding ability as well as reducing power, which may explain in part the mechanism behind the extract's ability to protect cells from oxidative damage. In addition, the extract also exhibited concentration-dependent antioxidant activities against hemoglobin-induced linoleic acid peroxidation and Fenton reaction-mediated plasmid DNA oxidation.

scholarly journals Assessment of Antioxidant Activity of Pure Graphene Oxide (GO) and ZnO-Decorated Reduced Graphene Oxide (rGO) Using DPPH Radical and H2O2 Scavenging Assays

2019 ◽  
Vol 5 (4) ◽  
pp. 75 ◽  
Author(s):  
Nacera Baali ◽  
Assia Khecha ◽  
Aicha Bensouici ◽  
Giorgio Speranza ◽  
Noudjoud Hamdouni
Keyword(s):  
Antioxidant Activity ◽  
Zinc Oxide ◽  
Graphene Oxide ◽  
Antioxidant Activities ◽  
In Vitro Assays ◽  
Dependent Manner ◽  
Mass Ratio ◽  
Dpph Radical ◽  
Scavenging Efficiency

In this work, zinc oxide-decorated graphene oxide (ZnO–rGO) was successfully synthesized with a fast reflux chemical procedure at 100 °C. An equal mass ratio of graphene oxide (GO) and zinc acetate was used as starting materials dissolved, respectively, in ultrapure distilled water and dimethylformamide (DMF). Particularly, pure GO was synthesized using Hummers modified protocol by varying the mass ratio of (graphite:potassium permanganate) as follows: 1:2, 1:3, and 1:4, which allow us to obtain six types of pure and decorated samples, named, respectively, GO1:2, GO1:3, GO1:4, ZnO–rGO1:2, ZnO–rGO1:3, and ZnO–rGO1:4 using reflux at 100 °C. X-ray diffraction, FTIR, and Raman spectroscopy spectra confirm the formation of wurzite ZnO in all ZnO-decorated samples with better reduction of GO in ZnO–rGO1:4, confirming that a higher degree of graphene oxidation allows better reduction during the decoration process with ZnO metal oxide. Antioxidant activity of pure and zinc oxide-decorated graphene oxide samples were compared using two different in vitro assays (DPPH radical and H2O2 scavenging activities). Considerable in vitro antioxidant activities in a concentration-dependent manner were recorded. Interestingly, pristine GO showed more elevated scavenging efficiency in DPPH tests while ZnO-decorated GO was relatively more efficient in H2O2 antioxidant assays.

scholarly journals Isolation of some compounds from nutmeg and their antioxidant activities

2012 ◽  
Vol 30 (No. 2) ◽  
pp. 164-170 ◽  
Author(s):  
J.P. Hou ◽  
H. Wu ◽  
Y. Wang ◽  
X.C. Weng
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Activities ◽  
Reducing Power ◽  
Stability Index ◽  
Natural Antioxidant ◽  
Synthetic Antioxidants ◽  
Oil Stability Index ◽  
Antioxidant Agent ◽  
Myristica Fragrans Houtt ◽  
First Time

Six compounds, licarin-B (1), dehydrodiisoeugenol (2), malabaricone B (3), malabaricone C (4), &beta;-sitosterol (5), and daucosterol (6) were isolated from the seed (nutmeg) of Myristica fragrans Houtt and identified. Among them, malabaricone B was identified for the first time in nutmeg. Antioxidant activities of the isolated compounds were studied using oil stability index (OSI), reducing power, ABTS<sup>●+</sup> scavenging, and DPPH<sup>●</sup> scavenging methods. The results showed that Malabaricone C is an efficient antioxidant agent which exhibits a stronger antioxidant activity than the commonly used synthetic antioxidants in all studied methods. This compound may have a potential to be used as a natural antioxidant in food.

scholarly journals Acetylcholinesterase Inhibitory and Antioxidant Activity of the Compounds Isolated from Vanda roxburghii

2021 ◽  
Vol 2021 ◽  
pp. 1-9
Author(s):  
Salim Ahammed ◽  
Rejina Afrin ◽  
Nasim Uddin ◽  
Yusuf Al-Amin ◽  
Kamrul Hasan ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Nervous System ◽  
Antioxidant Activities ◽  
Reducing Power ◽  
Chloroform Fraction ◽  
Total Antioxidant Activity ◽  
Traditional Use ◽  
Phytochemical Investigation ◽  
Total Antioxidant ◽  
First Time

Vanda roxburghii has been used in traditional medicine to treat nervous system disorders including Alzheimer’s disease (AD). We reported earlier a high acetylcholinesterase inhibitory and antioxidant activity in the chloroform fraction of this plant. Therefore, this study was designed to explore the compounds with acetylcholinesterase inhibitory and antioxidant activities from the chloroform fraction of Vanda roxburghii. Phytochemical investigation led to the isolation for the first time of a fatty acid ester: methyl linoleate (1), and three phenolics: syringaldehyde (2), vanillin (3), and dihydroconiferyl dihydro-p-coumarate (4) along with the previously reported compound gigantol (5). Among the isolates, vanillin (3) and dihydroconiferyl dihydro-p-coumarate (4) were found to significantly inhibit the activity of acetylcholinesterase, scavenge the free radicals, exhibit the reducing power and total antioxidant activity, and effectively reduce the peroxidation of lipid. Gigantol (5) and syringaldehyde (2), despite lacking the activity against acetylcholinesterase, exhibited antioxidant activity. Among the compounds, gigantol (5) appeared to be the most potent antioxidant. These findings revealed that V. roxburghii contained compounds with potential acetylcholinesterase inhibitory and antioxidant activity, which support its traditional use in the treatment of AD.

Antioxidant, Anti-Inflammatory and Cytotoxicity Properties of Centaurea africanaLamk var. [Bonnet] M

2020 ◽  
Vol 19 ◽  
Author(s):  
Sabrina Hadjira ◽  
Amira Mansour ◽  
Caglar Berke ◽  
Ramdane Seghiri ◽  
Ahmed Menad ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Cell Lines ◽  
Antioxidant Activities ◽  
Reducing Power ◽  
Phytochemical Analysis ◽  
Proliferative Potential ◽  
Myeloperoxidase Activity ◽  
Dependent Manner ◽  
Scavenging Effect ◽  
Anti Inflammatory

Background: In Algerian traditional medicine, Centaurea species are well known in traditherapy. The Centaurea africana has been used in folk medicine for the treatment of several inflammatory disorders. Objective: This study aims to examine the antioxidant, anti-inflammatory and anti-proliferative potential of both nButanol (BECA) and ethyl acetate (EAECA) extracts of Centaurea africana. Methods: The phytochemical analysis of both BECA and EAECA were explored and the antioxidant activities were investigated by measuring the DPPH° scavenging effect, the reducing power and the inhibition of lipid peroxidation (LPO) induced by Fe2+/ascorbic acid system. The anti-inflammatory properties were determined by measuring the NO° scavenging effect and by using carrageenan-induced rat paw oedema. The antiproliferative activity was studied on HT29 (human colorectal adenocarcinoma), OV2008 (human ovarian cancer) and C6 (Rattus norvegicus brain glioma) cell lines using the Sulforhodamine B assay. Results: The total polyphenol contents (TPC) of EAECA and BECA are recorded at 125.24±10.14 and 53.03±2.50 mgGAE/g extract, respectively. Both extracts revealed the antioxidant activity in a concentration-dependent manner; this effect is more pronounced with EAECA. The BECA exhibited a higher anti-inflammatory activity.This antiinflammatory activity was reflected in a reduction of swelling of carrageenan-evoked edemas (48.45 %), inhibition of nitric oxide (84.7 %), effective decrease in myeloperoxidase activity (58.82 %) and malondialdehyde level (65.58 %). The cytotoxic effect of BECA was found to be more pronounced against C6 cell lines (IC50 value: 131.93 µg/mL) while the cytotoxic activity of EAECA was more effective against HT29 and OV2008 cell lines. Conclusion: The obtained results indicated that EAECA exhibited a high antioxidant activity while BECA has a significant anti-inflammatory activity. Both extracts showed cytotoxic effects against cancer cell lines at certain concentrations in a cell-specific manner.

scholarly journals Antioxidant activity of rye caryopses and embryos extracts

2011 ◽  
Vol 20 (No. 6) ◽  
pp. 209-214 ◽  
Author(s):  
M. Karamać ◽  
R. Amarowicz ◽  
S. Weidner ◽  
S. Abe ◽  
F. Shahidi
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Antioxidant Activities ◽  
Reducing Power ◽  
Beta Carotene ◽  
Uv Spectra ◽  
Model System ◽  
Total Phenolic ◽  
Dpph Radical ◽  
Scavenging Effect

Phenolic compounds were extracted with 80% methanol from caryopses and embryos of rye (cv. Dańkowskie Złote and Amilo). In all extracts, reducing power, scavenging effect on DPPH radical, and antioxidant activity in a &beta;-carotene-linoleate model system were examined. The highest content of total phenolic compounds was noted in the extract from caryopses of Amilo (7.93 mg/g of extract). UV spectra of all extracts were characterised by maxima originated from phenolic acids (320, 326 and 328 nm), and by maxima at shorter wavelengths (272 and 274 nm) attributed to other phenolic compounds. All extracts showed a good antioxidant activity in a &beta;-carotene-linoleate model system. This activity was similar to that reported before in leguminous seeds extracts. The antioxidant activities of the extracts from the caryopses of Dańkowskie Złote and the embryos of both cultivars were very similar, especially during the second part of the incubation period. The extract of Amilo embryos showed a slightly weaker antioxidative effect. The weak antiradical effects in the experiments with DPPH radical and a weak reducing power were characteristic for all the extracts investigated. &nbsp;

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