scholarly journals Acetylcholinesterase Inhibitory and Antioxidant Activity of the Compounds Isolated from Vanda roxburghii

2021 ◽  
Vol 2021 ◽  
pp. 1-9
Author(s):  
Salim Ahammed ◽  
Rejina Afrin ◽  
Nasim Uddin ◽  
Yusuf Al-Amin ◽  
Kamrul Hasan ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Nervous System ◽  
Antioxidant Activities ◽  
Reducing Power ◽  
Chloroform Fraction ◽  
Total Antioxidant Activity ◽  
Traditional Use ◽  
Phytochemical Investigation ◽  
Total Antioxidant ◽  
First Time

Vanda roxburghii has been used in traditional medicine to treat nervous system disorders including Alzheimer’s disease (AD). We reported earlier a high acetylcholinesterase inhibitory and antioxidant activity in the chloroform fraction of this plant. Therefore, this study was designed to explore the compounds with acetylcholinesterase inhibitory and antioxidant activities from the chloroform fraction of Vanda roxburghii. Phytochemical investigation led to the isolation for the first time of a fatty acid ester: methyl linoleate (1), and three phenolics: syringaldehyde (2), vanillin (3), and dihydroconiferyl dihydro-p-coumarate (4) along with the previously reported compound gigantol (5). Among the isolates, vanillin (3) and dihydroconiferyl dihydro-p-coumarate (4) were found to significantly inhibit the activity of acetylcholinesterase, scavenge the free radicals, exhibit the reducing power and total antioxidant activity, and effectively reduce the peroxidation of lipid. Gigantol (5) and syringaldehyde (2), despite lacking the activity against acetylcholinesterase, exhibited antioxidant activity. Among the compounds, gigantol (5) appeared to be the most potent antioxidant. These findings revealed that V. roxburghii contained compounds with potential acetylcholinesterase inhibitory and antioxidant activity, which support its traditional use in the treatment of AD.

scholarly journals An Evaluation of the Antioxidant Activity of a Methanolic Extract of Cucumis melo L. Fruit (F1 Hybrid)

Separations ◽  
2021 ◽  
Vol 8 (8) ◽  
pp. 123
Author(s):  
R. S. Rajasree ◽  
Sibi P. Ittiyavirah ◽  
Punnoth Poonkuzhi Naseef ◽  
Mohamed Saheer Kuruniyan ◽  
G. S. Anisree ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Antioxidant Activity ◽  
Cucumis Melo ◽  
Methanolic Extract ◽  
Antioxidant Activities ◽  
Reducing Power ◽  
Total Antioxidant Activity ◽  
F1 Hybrid ◽  
Total Antioxidant ◽  
Cucumis Melo L

Cucumis melo L. (C. melo) is a fruit with many medicinal properties and is consumed in various countries. It is utilised for chronic eczema and to treat minor burns and scrapes. The present study was conducted to evaluate the antioxidant activity of a methanolic extract of Cucumis melo Linn (MECM). A coarse powder prepared from the fruit and seeds was extracted with methanol (absolute) by a hot continuous percolation process in accordance with the standard protocols. All the extracts were estimated for potential antioxidant activities with tests such as an estimation of total antioxidant activity, hydroxyl radical and nitric oxide scavenging activity and reducing power ability. The qualitative analysis of the methanolic extract of C. melo fruit showed the presence of various phytochemical constituents such as carbohydrates, alkaloids, sterols, phenolic compounds, terpenes and flavonoids. The total antioxidant activity of concentrations of 50, 100 and 200 µg were tested and observed to be 3.3 ± 0.1732, 6.867 ± 0.5457 and 13.63 ± 0.8295 µg of ascorbic acid, respectively. The results also showed significant nitric oxide and DPPH scavenging activities as well as a reducing power activity of MECM. Thus, our results suggest that MECM may serve as a putative source of natural antioxidants for therapeutic and nutraceutical applications.

scholarly journals Variation in antioxidant activity at two ripening stages of wild mango, Spondias pinnata (L.f.) Kurz., an underutilized fruit

2020 ◽  
Vol 7 (4) ◽  
Author(s):  
Bince Mani ◽  
Sinjumol Thomas
Keyword(s):  
Nitric Oxide ◽  
Hydrogen Peroxide ◽  
Antioxidant Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Total Phenolic ◽  
Total Antioxidant Activity ◽  
Plant Origin ◽  
Nitric Oxide Radical ◽  
Total Antioxidant

Antioxidant compounds play a significant role in preventing and scavenging free-radicals by reducing oxidative stress and providing protection to humans against degenerative diseases and infections. Obviously, antioxidant molecules of plant origin are pivotal to combat the oxidative harm in cells. Present work intended to evaluate the antioxidant capacity of the fruits of Spondias pinnata. Methanol extracts of the fruits at two stages of maturity were prepared and investigated by various antioxidants analyses such as total antioxidant activity, reducing power and radical scavenging assays (DPPH, nitric oxide and hydrogen peroxide). Its total phenol, flavonoid and tannin contents were also determined. Spondias pinnata fruit extracts exhibited effective antioxidant activity and its IC50 values of the unripe fruits were 65, 66, 72.23, 83.25, 66.75 ?g/ml and ripe fruits were 124.24, 92.50, 97.66, 144.10, 72.25 ?g/ml, for total antioxidant activity, reducing power, DPPH radical scavenging, nitric oxide radical scavenging and hydrogen peroxide scavenging assays respectively. The extracts, especially unripe fruit extract had good amounts of total phenolic and flavonoid contents which might contribute the antioxidant activities considerably. It is evident from the study that the fruits of S. pinnata possessed potent antioxidant activity and it can be considered as a good dietary choice among the underutilized fruits as well as common fruits. Therefore, wild mango may be a good choice of antioxidants of plant origin for dietary and pharmaceutical uses.

scholarly journals Synthesis, Characterization, and Antioxidant Activity of 8-Hydroxygenistein

2020 ◽  
Vol 15 (1) ◽  
pp. 1934578X2090139 ◽  
Author(s):  
Jin Shao ◽  
Tong Zhao ◽  
Hui-Ping Ma ◽  
Zheng-Ping Jia ◽  
Lin-Lin Jing
Keyword(s):  
Antioxidant Activity ◽  
High Performance ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Positive Control ◽  
Raw Material ◽  
Biochanin A ◽  
Total Antioxidant Activity ◽  
Nitric Oxide Radical ◽  
Total Antioxidant

It was reported that 8-hydroxygenistein (8-OHG) was synthesized by methylation, bromination, methoxylation, and demethylation using cheap and readily available biochanin A as raw material. All synthesized products were structurally confirmed by ultra-high-performance liquid chromatography (UHPLC), infrared spectroscopy, mass spectrometry, 1H-nuclear magnetic resonance (NMR), and 13C-NMR. In addition, we examined the antioxidant capacity of 8-OHG using 6 different methods such as 1,1-diphenyl-2-picrylhydrazyl radical scavenging, 2,2′-azinobis(3-ethylbenzothiazoline-6-sulfonate) radical (ABTS) scavenging, nitric oxide radical (NO) scavenging, superoxide radical (O2 −•) scavenging, reducing power assay, and total antioxidant activity using ascorbic acid (VC) as a positive control. Compared with VC, 8-OHG exhibited higher total antioxidant activity and stronger scavenging activity on ABTS, NO, and O2 −•. These results indicate that 8-OHG is an excellent antioxidant agent and may be effective in preventing damage induced by free radical.

scholarly journals Assessment of Physicochemical Quality, Antioxidant Content and Activity, and Inhibition of Cholinesterase between Unripe and Ripe Blueberry Fruit

Foods ◽  
2020 ◽  
Vol 9 (6) ◽  
pp. 690 ◽  
Author(s):  
Hyesung Hwang ◽  
Young-Jun Kim ◽  
Youngjae Shin
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Activities ◽  
Total Antioxidant Activity ◽  
Unripe Fruit ◽  
Potential Health ◽  
Total Anthocyanin ◽  
Food Ingredients ◽  
Physicochemical Quality ◽  
Total Antioxidant ◽  
Unripe Fruits

Five Korean blueberries (’’Nelson’’, ’’Duke ’’, ’’Bluejay ’’, ’’Toro’’, and ’’Elliot ’’) were harvested at two maturity stages (unripe and ripe) to evaluate fruit quality and antioxidant activities. The Hunter L, a, and b color of ripe blueberries was lower than that of unripe fruit. Soluble solid concentration (SSC) and pH increased, and titratable acidity (TA) and firmness decreased as the blueberries matured. The ripe blueberry fruits showed a higher SSC/TA ratio than the unripe fruits. Although total anthocyanin, flavonoids, phenolics content, and antioxidant activity were higher in ripe blueberries than in unripe fruit, the unripe fruit had higher acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) inhibition activities than ripe fruit in all cultivars. Total antioxidant activity was highly correlated with total flavonoids and phenolics. The relationships between the total antioxidant activity and the AChE or BChE inhibitory activity are negative. There were several physicochemical quality and antioxidant activity differences in blueberries, depending on the cultivar and the maturity at harvest. Unripe fruits also contain potential health-promoting bioactive compounds as functional food ingredients.

scholarly journals The Role of C-8 OH on the Antioxidant Activity of Norwogonin and Isowogonin

2020 ◽  
Vol 15 (5) ◽  
pp. 1934578X2092488
Author(s):  
Qiushan Zhang ◽  
Jin Shao ◽  
Tong Zhao ◽  
Lei He ◽  
Huiping Ma ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Ferrous Iron ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Iron Chelation ◽  
Reducing Power ◽  
Antioxidant Property ◽  
Hydroxyl Group ◽  
Total Antioxidant

In the present study, the antioxidant property of 4 flavones (moslosooflavone, wogonin, isowogonin, and norwogonin) was evaluated using 6 different assays: 1,1-diphenyl-2-picrylhydrazyl (DPPH·), superoxide (O2 •−), and nitric oxide (NO) radical scavenging assays, ferrous iron chelation, reducing power, and total antioxidant capacity. The 4 flavones exhibited antioxidant activities with decreasing order as norwogonin > isowogonin >> wogonin> moslosooflavone. The present results demonstrated that norwogonin and isowogonin exhibited excellent antioxidant activity, which was mainly based on the presence of C-8 hydroxyl group.

scholarly journals Studies on In Vitro Antioxidant Activity of Methanolic Extract and Fractions of Ficus Hispida Lin. Fruits

2016 ◽  
Vol 8 (3) ◽  
pp. 371-380 ◽  
Author(s):  
M. S. Hossain ◽  
S. Parvin ◽  
S. Dutta ◽  
M. S. I. Mahbub ◽  
M. E. Islam
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Total Phenolic ◽  
Scavenging Activity ◽  
Traditional Use ◽  
Total Antioxidant ◽  
Ficus Hispida

The present study was designed to confirm the traditional use of the fruits of Ficus hispida Linn. (Moraceae) as an antioxidant agent. Fruits of the plant extracted with methanol and crude methanol extract (CME) were further fractionated with n-hexane, chloroform, and ethyl acetate. All the fractions, n-hexane (NHF), chloroform (CHF), ethyl acetate (EAF), aqueous (AQF) and CME were preliminary screened for in vitro antioxidant activity and total phenolic and total flavonoid content. In DPPH radical scavenging assay, CME exhibited highest scavenging activity (IC50 = 11.20 µg/mL) as compared to other fractions. In this assay, IC50 of reference standard BHT was 5.10 µg/mL. The reducing power of the samples was in the order as AQF > CME > CHF > EAF > NHF. The results for hydrogen peroxide scavenging activity indicated that CME, EAF and AQF had almost the same scavenging activity except NHF. Total antioxidant capacity of CME and other fractions were ranked as CHF > AQF > CME > EAF > NHF.  In the assay of antioxidant constituents (total phenol and total flavonoids content), the CME had highest phenolic and flavonoids content. The results indicate that Ficus hispida fruits could be considered as a potential source of natural antioxidant.

scholarly journals Antioxidant and free radical scavenging effects of Iraqi sumac ( Rhus coriaria L)

2013 ◽  
Vol 10 (3) ◽  
pp. 921-933 ◽  
Author(s):  
Baghdad Science Journal
Keyword(s):  
Antioxidant Activity ◽  
Metal Ion ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Total Phenolic ◽  
Total Antioxidant ◽  
Rhus Coriaria ◽  
Dpph Radical Scavenging ◽  
Seed Extracts

This study was carried out to evaluate the antioxidant activity of Iraqi sumac seeds (Rhus coriaria. L) (Anacardiaceae). Total phenolic compounds and flavoniods were determined in three different sumac seed extracts (SSE) (aqueous,ethanolic and methanolic extract). For extraction Antioxidant activity of SSE were evaluated by various antioxidant assays, including total antioxidant capacity, reducing power,by using 1,1-diphenyl-2-picryl hydrazyl (DPPH) radical scavenging, nitric oxide scavenging, Hydroxyl radical scavenging, and metal ion chelating activities. These various antioxidant activities were compared with ascorbic acid as a standard antioxidant.The results showed that the three(SSE), contained large amounts of phenolic and flavoniods compounds. The results also showed that the three( SSEs ) have antioxidant activities , the methanolic (SSEs) contain larger amounts of phenolic and flavoniod compounds and have a higher antioxidant activities than that of aqueous or ethanolic (SSE).These results indicated that Iraqi aqueous and alcoholic extracts of Iraqi sumac in especial methanolic) SSE( are good scavengers for reactive oxygen species(ROS) and are a potential source of natural antioxidant, that may be used in pharmaceutical or food industry .

scholarly journals Screening of Selected Sri Lankan Seasonal Fruit Extracts for Total Antioxidant Activity in vitro

2019 ◽  
Vol 7 (1) ◽  
pp. 7-12
Author(s):  
Amarosige Shanoli Kavindya Fernando ◽  
Anoja Priyadarshani Attanayake ◽  
Kamani Ayoma Perera Wijewardena Jayatilaka
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Activities ◽  
Dry Weight ◽  
Total Antioxidant Activity ◽  
Polyphenol Content ◽  
Total Polyphenol ◽  
Fruit Extracts ◽  
Total Polyphenol Content ◽  
Total Antioxidant

The present study aimed to determine the total polyphenol content and total antioxidant activities of ten selected seasonal fruit extracts grown in Sri Lanka. The aqueous refluxed fruit extracts (4h) were used at the initial concentration of 0.05 g/mL. The total polyphenol content was determined according to the Folin-Ciocalteu method. The total antioxidant activity was evaluated by DPPH (2, 2-diphenyl-2-picrylhydrazyl), FRAP (ferric reducing power) and TBA (thiobabituric acid assay) assays with L-ascorbic acid as the reference compound. The total polyphenol content of the fruit extracts varied from 0.81±0 to 17.54±0.50 mg GAE (gallic acid equivalents) per gram of dry weight. The antioxidant activities ranged in IC50 of 46.60±0.60 to 367.90 ± 4.90 μg/mL, 3.41±0.02 to 50.46±1.02 µM, 30.62±0.50 to 182.64±0.90 mg AAE (L-ascorbic acid equivalents) per gram of dry weight for DPPH, FRAP, TBA assays, respectively. All aqueous fruit extracts exert dose dependent in vitro antioxidant activities in different degrees. Among the selected fruit extracts S. carophylatum, P. zeylanica, A. comosus exert relatively high total antioxidant activity together with high total polyphenol content. Hence, the selected fruit extracts are deserved to be as potent sources for antioxidant lead compounds in the development of nutraceuticals using the above edible fruits.

scholarly journals Antioxidant activity of phenolic fractions of Mallotus philippinensis bark extract

2009 ◽  
Vol 27 (No. 2) ◽  
pp. 109-117 ◽  
Author(s):  
M. Arfan ◽  
H. Amin ◽  
M. Karamać ◽  
A. Kosińska ◽  
W. Wiczkowski ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Antiradical Activity ◽  
Total Phenolics ◽  
Reducing Power ◽  
Solvent System ◽  
Bark Extract ◽  
Total Antioxidant Activity ◽  
Phenolic Constituents ◽  
Total Antioxidant ◽  
Fraction I

Phenolic compounds were extracted from <I>Mallotus philippinensi</I> bark using methanol. Six fractions (I–VI) were separated from the extract on a Sephadex LH-20 column using ethanol and acetone-water as the mobile phases and were evaluated for their total antioxidant activity, antiradical activity against DPPH<sup>•</sup> (2,2-diphenyl-1-picrylhydrazyl radical), and reducing power. The total phenolics and tannin contents in the fractions were determined. The content of total phenolics in the fractions ranged from 54 mg/g (fraction I) to 927 mg/g (fraction VI). Condensed tannins were detected in fractions II–VI. Total antioxidant activity (TAA) of phenolic fractions of <I>Mallotus philipinensis</I> bark extract ranged from 0.58 mmol Trolox/g (fraction I) to 6.82 mmol Trolox/g (fraction IV). Fraction IV also showed the strongest antiradical activity against DPPH<sup>•</sup> and reducing power. Several phenolic constituents in the fractions were detected by RP-HPLC using a gradient solvent system with UV-DAD detection.

scholarly journals PHYTOCHEMICAL SCREENING AND EVALUATION OF LEAF PARTS OF HYPOESTES PHYLLOSTACHYA ROSEA FOR IN VITRO ANTIOXIDANT ACTIVITIES

2020 ◽  
pp. 54-58
Author(s):  
JENSON JACOB ◽  
MEENU JOHN
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Activities ◽  
Standard Procedure ◽  
Reducing Power ◽  
Phytochemical Analysis ◽  
Soxhlet Apparatus ◽  
Total Antioxidant ◽  
Phytochemical Compounds ◽  
Reducing Power Assay

Objective: The aim of this study was focussed on phytochemical analysis, in vitro antioxidant activities of different solvent extracts of Hypoestes phyllostachya leaf. Methods: The leaves were washed efficiently, shade dried and coarsely powdered. The powdered leaf material of Hypoestes phyllostachya was successively extracted with hexane, chloroform, methanol and water using Soxhlet apparatus. Preliminary phytochemical screenings for polyphenolics, proteins, phytosteroids, flavonoids, tannins, glycosides, alkaloids carbohydrates, polyphenolics, saponins were done by following standard procedure. In vitro antioxidant activities of various solvent extracts were assessed using DPPH, ABTS, Hydroxy radical, total antioxidant capacity and reducing power assay. Polyphenolics and flavonoids were also estimated. Results: The results showed that most of the phytochemical compounds such as carbohydrates, proteins, steroids, fixed oils and fatty acids, flavonoids, alkaloids, saponins, tannins, polyphenolics and glycosides present in the methanolic and aqueous extract of Hypoestes phyllostachya. The in vitro antioxidant activity of Hypoestes phyllostachya leaf clearly showed that it has better antioxidant activity, which was confirmed by different assays. Conclusion: From this work, it can be concluded that Hypoestes phyllostachya has the potential to be a good antioxidant but have only a lesser amount of flavonoids and polyphenolic contents. This was the first report on the pharmacological activity of the Hypoestes phyllostachya.

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