scholarly journals Synthesis and Characterization of Bacterial Cellulose - Garcinia mangostana Extract as Anti Breast Cancer Biofilm Candidate

2017 ◽  
Vol 30 ◽  
pp. 76-85 ◽  
Author(s):  
Waya Rahmaning Gusti Agrippina ◽  
Prihartini Widiyanti ◽  
Helmy Yusuf
Keyword(s):  
Breast Cancer ◽  
Cancer Cells ◽  
Bacterial Cellulose ◽  
Mtt Assay ◽  
Breast Cancer Cells ◽  
Ethanol Extract ◽  
Ethanol Solution ◽  
Garcinia Mangostana ◽  
Mangosteen Peel ◽  
Peel Extract

In Indonesia, breast cancer is noted as the most common cancer in women. Accordingly, this research was conducted to synthesize biofilm from bacterial cellulose by adding ethanol extract of mangosteen peel. The pellicle of bacterial cellulose was soaked in a 100 mL ethanol solution of mangosteen peel extract varied by 0.5%, 1%, 1.5%, and 2% v/v. The samples were characterized using the SEM, FTIR, and MTT Assay using the T47D breast cancer cells. The results using the SEM showed the thickness of the bacterial cellulose biofilm samples was 5.63 μm, while the 2% v/v thickness of the bacterial cellulose of the extract of mangosteen peel biofilm samples was 12.2 μm. The FTIR results showed a weak interaction between the O-H groups of the microbial cellulose and the C=C functional group in the phenolic compounds of the mangosteen peel extract. Based on the MTT Assay test results using the T47D breast cancer cells, the highest percentage of cell death result was 25.47% on the 2% v/v bacterial cellulose of the mangosteen peel extract samples. The Garcinia mangostana extracts added in the bacterial cellulose biofilms still required optimal concentrations in order to become potential killing mechanism for the T47D breast cancer cells.

scholarly journals Efek Sitotoksik Ekstrak Etanolik Ocimum basilicum, L. Pada Sel Kanker Payudara

2019 ◽  
Vol 6 (2) ◽  
pp. 74
Author(s):  
Devi Nisa Hidayati ◽  
Ibrahim Arifin ◽  
Fatimatuz Zahroh ◽  
Lina Wahyuni
Keyword(s):  
Breast Cancer ◽  
Cancer Treatment ◽  
Cytotoxic Activity ◽  
Cancer Cells ◽  
Mtt Assay ◽  
Breast Cancer Cells ◽  
Ethanol Extract ◽  
Ocimum Basilicum ◽  
Cytotoxic Test ◽  
Mcf 7

ABSTRAK Pengobatan kanker menggunakan bahan alam terus dikembangkan. Salah satu tanaman yang memiliki efek sitotoksik Ocimum basilicum, L. tujuan penelitian ini adalah mengetahui aktivitas sitotoksik dari ekstrak etanol Ocimum basilicum (EEOB) terhadap sel kanker payudara T47D dan MCF7. Ocimum basilicum diekstraksi menggunakan metode maserasi dengan pelarut etanol 70%. Pengujian aktivitas sitotoksik menggunakan metode MTT assay dengan  seri konsentrasi EEOB 1000; 500; 250; 125; 62.5; 31,25 µg/mL. Hasil uji aktivitas sitotoksik EEOB memperlihatkan nilai IC50 pada sel T47D dan MCF-7 sebesar 399.86 µg/ml dan 387.76 µg/ml. Kata Kunci—Sitotoksik, Ocinum basilicum L., T47D, MCF-7  ABSTRACT             Cancer treatment using natural ingredients continues to be developed. One of the plants that is proven to have cytotoxic activity is basil leaves (Ocimum basilicum, L.). This study aims to determine the cytotoxic activity of ethanol extract of basil leaves (EEBL) on T47D and MCF-7 breast cancer cells. Basil leaves were extracted using maceration  with ethanol 70%. The cytotoxic test was perfomed using MTT assay with various EEBL concentrations: 1000; 500; 250; 125; 62.5; 31,25 µg/mL. The results showed that IC50 of cytotoxic activity in T47D and MCF-7 was 399.86 µg/ml and 387.76 µg/ml respectively. Keywords—Cytotoxic, Ocinum basilicum L., T47D, MCF-7

scholarly journals Comparative effect of thermo/pH-responsive polymer-coated gold nanocages and hollow nanostars on chemo-photothermal therapy of breast cancer cells

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Asrin Pakravan ◽  
Mehdi Azizi ◽  
Fariborz Rahimi ◽  
Farhad Bani ◽  
Farideh Mahmoudzadeh ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cancer Cells ◽  
Mtt Assay ◽  
Photothermal Therapy ◽  
Breast Cancer Cells ◽  
Covalent Attachment ◽  
Mcf7 Cells ◽  
Dapi Staining ◽  
Hemolysis Assay ◽  
Gold Nanocages

Abstract Background Combination chemo-photothermal therapy appears to be one of the next generations of cancer treatment. In this study hollow gold nanostars (HGNSs) and gold nanocages (GNCs) were synthesized and stabilized with thermo-pH-sensitive thiol-end capped ABC triblock copolymer poly(acrylic acid)-b-poly(N isopropylacrylamide)-b-poly (e-caprolactone)-SH; PAA-b-PNIPAAm-b-PCL-SH (GNSs@Pol). Doxorubicin (Dox) was conjugated to the GNSs@Pol nanostructures via ionic interaction, covalent attachment and hydrogen bonding (GNSs@Dox-Pol). The physicochemical characteristics of prepared GNSs@Pol and GNSs were assessed using dynamic light scattering (DLS), transmission electron microscopy (TEM) and zeta potential techniques. Cytocompatibility of the GNSs@Pol was studied by hemolysis assay and MTT assay. The chemo-photothermal therapy (PTT) potential of GNSs@Dox-Pol was compared on MCF7 cells using MTT assay, cell cycle, DAPI staining and Annexin-V apoptosis assay techniques. Results Cell internalization results showed an almost complete uptake of GNSs@Pol by MCF-7 cells in the first 3 h of treatment. The heat generation measurement results showed that both of GNSs have a potential for light to heat conversion (∆T = 23–27 ºC) and HGNSs demonstrated better efficiency than GNCs after 10-min exposure to NIR irradiation. Following chemo-photothermal treatment, the highest cell mortality (90%) and apoptotic effects (97% apoptosis) were observed in HGNSs@Dox-Pol received laser irradiation treatment group. Conclusions This work highlights the potential application of designed GNSs@Dox-Pol in a combinational chemo-PTT to treat breast cancer cells. Graphic abstract

Antiglycolytic Activities of Strobilanthes crispus Active Fraction and its Bioactive Components on Triple-Negative Breast Cancer Cells In Vitro

2021 ◽  
Vol 21 ◽  
Author(s):  
Siti N.H. Muhammad ◽  
Nik S. Yaacob ◽  
Nur A.M. Safuwan ◽  
Agustine N. Fauzi
Keyword(s):  
Breast Cancer ◽  
Glucose Uptake ◽  
Cancer Cells ◽  
Mtt Assay ◽  
Breast Cancer Cells ◽  
Triple Negative ◽  
Lactate Concentration ◽  
Active Fraction ◽  
Bioactive Components ◽  
Strobilanthes Crispus

Background: Survival and progression of cancer cells are highly dependent on aerobic glycolysis. Strobilanthes crispus has been shown to have promising anticancer effects on breast cancer cells. The involvement of the glycolysis pathway in producing these effects is unconfirmed, thus further investigation is required to elucidate this phenomenon. Objective: This study aims to determine the effect of S. crispus active fraction (F3) and its bioactive components on glycolysis in triple-negative breast cancer cells (MDA-MB-231). Methods: This study utilizes F3, lutein, β-sitosterol, and stigmasterol to be administered in MDA-MB-231 cells for measurement of antiglycolytic activities through cell poliferation, glucose uptake, and lactate concentration assays. Cell proliferation was assessed by MTT assay of MDA-MB-231 cells after treatment with F3 and its bioactive components lutein, β-sitosterol, and stigmasterol. The IC50 value in each compound was determined by MTT assay to be used in subsequent assays. The determination of glucose uptake activity and lactate concentration were quantified using fluorescence spectrophotometry. Results: Antiproliferative activities were observed for F3 and its bioactive components, with IC50 values of 100 µg/mL (F3), 20 µM (lutein), 25 µM (β-sitosterol), and 90 μM (stigmasterol) in MDA-MB-231 cells at 48 h. The percentage of glucose uptake and lactate concentration in MDA-MB-231 cells treated with F3, lutein, or β sitosterol were significantly lower than those observed in the untreated cells in a time-dependent manner. However, treatment with stigmasterol decreased the concentration of lactate without affecting the glucose uptake in MDA-MB-231 cells. Conclusion: The antiglycolytic activities of F3 on MDA-MB-231 cells are attributed to its bioactive components.

scholarly journals In Vitro Cytotoxic Study and Detection of Apoptosis on Breast Cancer Cell lines MDA-MB 231 after Exposed to Azadirachta Indica A. Juss (neem) Extract

2013 ◽  
Vol 2 (2) ◽  
pp. 80 ◽  
Author(s):  
Dessy Arisanty
Keyword(s):  
Breast Cancer ◽  
Cell Death ◽  
Cancer Cells ◽  
Mtt Assay ◽  
Azadirachta Indica ◽  
Cell Apoptosis ◽  
Ethanol Extract ◽  
Tunel Assay ◽  
Neem Extract

AbstrakSuatu senyawa obat dapat menjadi kemoterapi kanker adalah dengan cara menskrining terlebih dahulu tumbuhan obat yang berpotensi sebagai obat antikanker. Salah satunya adalah tanaman obat daun nimba (Azadirachta indica L.Juss) yang terbukti secara significant menyebabkan apoptosis pada beberapa jenis sel line kanker. Dalam penelitian ini, ekstrak ethanol dari A. indica dipelajari untuk melihat efeknya pada pertumbuhan sel kanker payudara manusia jenis MDA-MB-231 dengan menggunakan tes untuk proliferasi yaitu MTT assai dan untuk mengetahui perubahan morphologi dari apoptosis selnya dengan menggunakan TUNEL assay Ekstrak daun nimba (A. indica) dapat menurunkan keberadaan jumlah sel kanker dengan cara menghambat perkembangan daripada sel tersebut dan menginduksi proses apoptosis pada sel kanker tersebut. Hasil pemeriksaan MTT assai didapatkan nilai IC50 nya adalah 55 ug / mL. Kematian MDA-MB231 sel yang disebabkan oleh ekstrak daun nimba (A.indica) ditemukan melalui mekanisme apoptosis yang secara morfologinya menunjukan ciri ciri dari kematian secara apoptosis seperti kondensasi dari nucleus, membrane nukleus yang melebur dan akhirnya terjadinya fragmentasi dari DNA. Analisis struktur dalaman sel juga mengungkapkan karakteristik apoptosis yaitu marginasi dari kromosom yang disertai dengan fragmentasi DNA dan selanjutnya akan terbentuk badan apoptotik pada sel kanker yang diinkubasi dengan ekstrak tersebut. Pada penelitian ini juga dijumpai peningkatan jumlah sel apoptosis dari hari 1 sampai hari 3 inkubasi oleh ekstrak nimba. Ekstrak ethanol A.indica mungkin mengandung senyawa bioaktif(s) yang menyebabkan kanker payudara MDA-MNB 231 mengalami kematian sel secara apoptosis. Penelitian lebih lanjut masih diperlukan untuk mengetahui mekanisme tumbuhan ini membunuh sel kanker MDA-MB 231.Kata kunci: Studi In vitro, Azadirachta indica, apoptosis, TUNEL assayAbstractA screening is conducted on plants that have potential as anticancer is a promising way for discovering novel chemotherapeutic compound. A medicinal plant neem leaf (Azadirachta indica L.Juss) intake has been shown to induce significant levels of apoptosis in various cancer cells. In this present study, ethanol extract of Azadirachta indica was studied for its effects on growth in MDA-MB 231 human breast cancer cells using assays for proliferation (MTT assay) and mechanisme of cell apoptosis using TUNEL assay. Neem leaf extract decreased cell viability, inhibited cell proliferation, and induced cell apoptosis. Result of MTT assay was 55 μg/mL of neem remarkably reduced cell viability of MDA-MB 231 cells. MDA-MB231 cell death elicited by the extract was found to be apoptotic in nature based the indication of nucleus condensation, shrinkage of nucleus membrane and also DNA fragmentation which are a hallmark of apoptosis. In addition, ultrastructural analysis also revealed apoptotic characteristics which are the presence of chromatin margination and apoptotic bodies in the extract-treated cells. There was an increase in the number of apoptotic cells from day 1 to day 3 post incubation with neem extract. Thus, the results from this study strongly suggest that the ethanol extract of A.indica may contain bioactive compound(s) that caused breast carcinoma, MDA-MNB 231 cell death by apoptosis. It’s needed to do advance research to know more deeply the mechanism this plant on breast cancer cell line MDA-MB.Keywords:In vitro study, Azadirachta indica, apoptosis, TUNEL assay

scholarly journals CYTOTOXIC EFFECT OF AQUADEST, ETHANOLIC, AND CHLOROFORM EXTRACTS OF Spathoglottis plicata Blume ON BREAST CANCER CELLS LINE (T47D CELLS)

2015 ◽  
Vol 2 (1) ◽  
pp. 64
Author(s):  
Mukhlish Jamal Musa Holle ◽  
Hestri Dyah Puspitasari ◽  
Andaru Satryo ◽  
Wahyu Dewi Astuti Ningrum ◽  
Digdo Sudigyo ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cytotoxic Activity ◽  
Cancer Cells ◽  
Mtt Assay ◽  
Breast Cancer Cells ◽  
Chloroform Extract ◽  
Probit Analysis ◽  
Antioxidant Compounds ◽  
T47d Cells ◽  
Ic50 Value

Breast cancer is one of cancer with high mortality. This cancer not only attacks women, but also men. Indonesia has many plants which potential as anticancer, such as orchids. Spathoglottis plicata is one of the orchid species that abundant in Indonesia and has a lot of antioxidant compounds which is guessed have anticancer properties. The objectives of this study were to study the cytotoxic activity and IC50 value of aquadest, ethanolic, and chloroform extracts of S. plicata’s pseudobulbs, leaves, and whole plants on T47D cells (breast cancer cells line) as well as cytotoxic activity of the specific fraction of the most toxic crude extract. S. plicata used in this study was obtained from Bungarinte nursery. Extractions were done by maceration method using aquadest, ethanol, and chloroform as the solvent. Cytotoxic test on T47D cells were done by MTT assay. The cytotoxic data were analyzed using one-way ANOVA followed by Tukey’s HSD test. The IC50 of each extracts were calculate by probit analysis. The lowest IC50 value among all extracts was fractionated and isolated by preparative TLC. The cytotoxic activity and IC50 of this fractions were analyzed. The results showed that only 2 from 9 crude extracts that able to calculate its IC50 because those two extracts have concentration dependent pattern of inhibition concentration. Chloroform extract have the lowest IC50 value (369,837 μg/mL). Then, this extract fractionated by eluen n-hexane : ethyl acetate 4:1. Four fractions were collected. The lowest IC50 value is fraction IV (144,41 μg/mL). Based on the results it could be concluded that S. plicata leaves have moderate potency to develop as anticancet agents, especially on breast cancer. Keywords: S. plicata, T47D cells, cytotoxic, MTT assay, preparative TLC. 

Exploring the Molecular Mechanism of Cinnamic Acid-Mediated Cytotoxicity in Triple Negative MDA-MB-231 Breast Cancer Cells

2020 ◽  
Vol 20 ◽  
Author(s):  
Ambika Pal ◽  
Poulami Tapadar ◽  
Ranjana Pal
Keyword(s):  
Breast Cancer ◽  
Dna Damage ◽  
Cell Death ◽  
Cancer Cells ◽  
Cinnamic Acid ◽  
Mtt Assay ◽  
Trypan Blue ◽  
Breast Cancer Cells ◽  
Triple Negative ◽  
Caspase 8

Background: Cinnamic acid (CA), also known as 3-phenyl-2-propenoic acid, is a naturally occurring aromatic fatty acid found commonly in cinnamon, grapes, tea, cocoa, spinach and celery. Various studies have identified CA to have anti-proliferative action on glioblastoma, melanoma, prostate and lung carcinoma cells. Objective: Our objective was to investigate the molecular mechanism underlying the cytotoxic effect of CA in killing MDA-MB-231 triple negative breast cancer cells. Methods: We performed MTT assay and trypan blue assay to determine cell viability and cell death, respectively. Comet analysis was carried out to investigate DNA damage of individual cells. Furthermore, AO/EtBr assay and sub-G1 analysis using flowcytometry was used to study apoptosis. Protein isolation followed by immunoblotting was used to observe protein abundance in treated and untreated cancer cells. Results: Using MTT assay we have determined CA to reduce cell viability in MDA-MB-231 breast cancer cells and tumorigenic HEK 293 cells but not in normal NIH3T3 fibroblast cells. Subsequently, trypan blue assay and comet assay showed CA to cause cell death and DNA damage, respectively, in the MDA-MB-231 cells. Using AO/EtBr staining and sub-G1 analysis we further established CA to increase apoptosis. Additionally, immunoblotting showed the abundance of TNFA, TNF receptor 1 (TNFR1) and cleaved caspase-8/-3 pro-apoptotic proteins to increase on CA treatment. Subsequently, blocking of TNFA-TNFR1 signalling by small molecule inhibitor, R-7050, reduced the expression of cleaved caspase-8 and caspase-3 at the protein level. Conclusion: Thus, from the above observations we can conclude that CA is an effective anticancer agent that can induce apoptosis in breast cancer cells via TNFA-TNFR1 mediated extrinsic apoptotic pathway.

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