The Effect of Pore Forming Agent Particle Size on the Porosity, Microstructure and In Vitro Studies of Hydroxyapatite Ceramics

2011 ◽  
Vol 493-494 ◽  
pp. 277-280 ◽  
Author(s):  
V. Zalite ◽  
Janis Locs ◽  
D. Vempere ◽  
Liga Berzina-Cimdina
Keyword(s):  
Particle Size ◽  
Fibroblast Cell ◽  
Particle Size Effect ◽  
Ammonium Bicarbonate ◽  
Porous Hydroxyapatite ◽  
Hydroxyapatite Ceramics ◽  
Ceramic Microstructure ◽  
Viscous Mass

In situ viscous mass foaming with ammonium bicarbonate (NH4HCO3)as foaming agent was used for the preparation of porous hydroxyapatite ceramics. Three different particle fractions (100-300 μm, 300-500 μm, 500-1000 μm) of NH4HCO3 were used in order to evaluate the particle size effect on the ceramic microstructure. Foamed and dried green bodies were sintered at 1150°C. The porosity and microstructure of ceramics were determined with Archimedes method and scanning electron microscopy. As the NH4HCO3 particle fraction was decrease from 100-300 μm to 500-1000 μm, the open porosity increased from 42 to 54 %. In situ viscous mass foaming leads to the interconnected pore channels with wide pore size range (10-600 μm). In the present work cytotoxicity of obtained porous HA ceramics and fibroblast cell viability was investigated.

Growth of osteoblast-like cells on porous hydroxyapatite ceramics: an in vitro study

2002 ◽  
Vol 19 (2-6) ◽  
pp. 119-124 ◽  
Author(s):  
L Cerroni ◽  
R Filocamo ◽  
M Fabbri ◽  
C Piconi ◽  
S Caropreso ◽  
...  
Keyword(s):  
In Vitro Study ◽  
Vitro Study ◽  
Porous Hydroxyapatite ◽  
Hydroxyapatite Ceramics

Particle size effect for cobalt Fischer–Tropsch catalysts based on in situ CO chemisorption

2016 ◽  
Vol 648 ◽  
pp. 67-73 ◽  
Author(s):  
Jia Yang ◽  
Vidar Frøseth ◽  
De Chen ◽  
Anders Holmen
Keyword(s):  
Particle Size ◽  
Size Effect ◽  
Particle Size Effect ◽  
Fischer Tropsch ◽  
Co Chemisorption

Physically effectiveness of beet pulp in dairy cows 1: physical characteristics, ruminal kinetics of nutrients degradation, hydration, and functional specific gravity

2007 ◽  
Vol 2007 ◽  
pp. 215-215
Author(s):  
Teimouri Yansari Asadollah
Keyword(s):  
Particle Size ◽  
Specific Gravity ◽  
Physical Characteristics ◽  
Particle Size Measurement ◽  
Effective Factor ◽  
Beet Pulp ◽  
Water Holding ◽  
Kinetics Of

Some physical properties including particle size, functional specific gravity (FSG; Teimouri Yansari et al., 2004), hydration rate, water holding capacity (WHC) and ionion-cation exchange (Van Soest, 1994) influenced on physically effective factor (pef), but only particle size measurement is central to all effective fibre systems. The objective of this study was to evaluate the effects of three types of beet pulp (BP) on physical characteristics including bulk density, particle size, kinetics of hydration, FSG, WHC, and intrinsic osmotic pressure that measured usingin vitroandin situmethods.

Porous hydroxyapatite ceramics coated with biomimetic peptides for induced osteogenesis

2020 ◽  
Vol 10 (9) ◽  
pp. 1524-1530
Author(s):  
Jing-Bo Xu ◽  
Fei Peng ◽  
Youlu Che ◽  
Wei Zhang ◽  
Changyun Quan
Keyword(s):  
Surface Modification ◽  
Bone Tissue ◽  
Physical Adsorption ◽  
Biological Properties ◽  
Porous Hydroxyapatite ◽  
Interfacial Activity ◽  
Hydroxyapatite Ceramics ◽  
Before And After ◽  
Effective Role

Biomimetic peptide has attracted extensive attention in bone tissue repairing owing to its excellent biocompatibility and stability. Hydroxyapatite ceramics (HAP) possess both excellent mechanical properties and good biocompatibility. To study the effects of bionic peptide D9KIPKAS(pSer)VPTELSAISRGDS on the interfacial activity and biological properties of hydroxyapatite ceramics, porous HAP ceramics were prepared using ammonium carbonate as a pore-forming agent. To explore the influence of surface modification on the interfacial activity of porous HAP ceramics when applying different methods, surface modification was carried out using physical adsorption (HAP-p-PP2) and a chemically grafted polypeptide (HAP-c-PP2). X-ray diffraction was used to characterize the crystal morphology of the porous HAP ceramics before and after sintering. The results of FTIR and XPS showed that bionic peptides were successfully grafted onto the surface of a porous HAP ceramic. An SEM graph shows the adhesion and spread of BMSCs on the materials. Meanwhile, the results of in vitro cell experiments showed that HAP-c-PP2 can better promote BMSC proliferation. In conclusion, bionic peptide D9KIPKAS(pSer)VPTELSAISRGDS with multifunctional functional groups is more conducive to the adhesion, proliferation and differentiation of BMSCs which can make it play an effective role in osteoinduction in bone tissue engineering.

scholarly journals Combined effects of melatonin and FGF-2 on mouse preosteoblast behavior within interconnected porous hydroxyapatite ceramics - in vitro analysis

2016 ◽  
Vol 24 (2) ◽  
pp. 153-161 ◽  
Author(s):  
Mohammad Zeshaan RAHMAN ◽  
Hideo SHIGEISHI ◽  
Kazuki SASAKI ◽  
Akira OTA ◽  
Kouji OHTA ◽  
...  
Keyword(s):  
Combined Effects ◽  
Porous Hydroxyapatite ◽  
Hydroxyapatite Ceramics ◽  
Vitro Analysis ◽  
In Vitro Analysis

scholarly journals Design of a Reversible Cholinesterase Inhibitor Mamentine HCL Nanogel: Formulation and In-vitro Evaluation

2021 ◽  
pp. 352-359
Author(s):  
Subhasri Mohapatra ◽  
Sourabh Jain ◽  
Karunakar Shukla
Keyword(s):  
Particle Size ◽  
Cholinesterase Inhibitor ◽  
Average Particle Size ◽  
In Vitro Release ◽  
Storage Period ◽  
Spherical Particles ◽  
Average Particle ◽  
In Situ Gel

Memantine hydrochloride is a is a reversible cholinesterase inhibitor used in the treatment of Alzheimer’s disease, low-moderate affinity, uncompetitive n-methyl-d-aspartate (NMDA) receptor antagonist, with strong voltage dependency and rapid blocking/unblocking kinetics. The present study was explore the potential of thermosensitive nanogel of mamentine loaded nanoparticle. In situ gel choosing due to restrict unwanted exposure in blood and other healthy tissues, thus eliminate hemolytic side effects of the drug and offer easy administration in vivo. Nanoparticle prepared by ionic gelation method and further the dried nanoparticle incorporates with in situ gel.  The in situ gel prepared by cold method using the solutions of Poloxamer-188 and Carbopol-934. The Transmission electron microscopy showed the spherical particles  with  smooth surface which was in conformity  with the SEM and Zetasizer  data for particle size. The pH of the formulations was found to be satisfactory and was in the range of 6.8±0.039 -7.4±0.053 and also mucoadhesive strength was show in table. The mucoadhesive strength of all formulations was varies from 2398±0.0004 to 4945±0.0002 dynes/cm2. In-vitro diffusion study of the in situ gel (N1-N8) was performed using modified Franz diffusion cell with dialysis membrane in phosphate buffer pH 6.5 for a period of 24 hours. The in vitro release study were fitted into various kinetic models viz zero order, first order, higuchi model and korsmeyer peppas equation. Stability studies for optimized formulations were carried out at 4.0 ± 0.5°C and 37 ± 0.5ºC for a period of four weeks. There was no significant variation found in physical appearance, average particle size and % drug content of the in situ nanogel N2. No visible changes in the appearance of the gel formulation were observed at the end of the storage period.

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