Characterization of Hydrothermal Processing Influence on Strontium Substituted Apatite by Investigating Stable Oxygen Isotope Ratio and Antibacterial Activity of Obtained Peroxyapatite

2018 ◽  
Vol 762 ◽  
pp. 14-18
Author(s):  
Valda Valkovska ◽  
Agnese Osīte ◽  
Lauma Buša ◽  
Vizma Nikolajeva
Keyword(s):  
Antibacterial Activity ◽  
Isotopic Ratio ◽  
Antibacterial Properties ◽  
Thermal Conversion ◽  
Hydrothermal Processing ◽  
Host Materials ◽  
Stable Oxygen ◽  
Synthetic Hydroxyapatite ◽  
Strontium Cation

Synthetic hydroxyapatite (HAp) has weak antibacterial and mechanical properties. The antibacterial activity of HAp can be enhanced by strontium cation substitution and incorporation of peroxide ion via hydrothermal processing at 100 °C and 150 °C temperature using 50% H2O2 solution. The starting reagents and products of HAp processing were analyzed by thermal conversion elemental analyser – isotopic ratio mass spectrometer in order to determine the δ18O values. Using different host materials it could be possible to determine differences of δ18O values between untreated and hydrothermally treated HAp samples. The enhanced antibacterial properties of processed HAp samples were investigated using Pseudomonas aeruginosa and Staphylococcus aureus. Hydrothermal processing by H2O2 solutions provides additional antibacterial activity and peroxide content of hydrothermally treated samples affect δ18O values.

scholarly journals Antibacterial Properties of Biofilm Schiff Base Derived from Dialdehyde Cellulose and Chitosan

2019 ◽  
Vol 19 (2) ◽  
pp. 405
Author(s):  
Agung Pratama ◽  
Firman Sebayang ◽  
Rumondang Bulan Nasution
Keyword(s):  
Tensile Strength ◽  
Antibacterial Activity ◽  
Schiff Base ◽  
Antibacterial Properties ◽  
Oxidized Cellulose ◽  
Natural Polymers ◽  
Dialdehyde Cellulose ◽  
Ft Ir ◽  
Ir Analysis

Cellulose and chitosan are natural polymers that have been used as biocomposite. The aim of this research is to obtain biofilms from chitosan and oxidized cellulose crosslinks. This research is divided into three steps, i.e., isolation of cellulose from oil palm trunk and oxidation of cellulose using NaIO4 (0.2; 0.4; 0.6; 0.8; 1.0 mg/mL) to obtain dialdehyde cellulose (DAC), crosslink of oxidized cellulose with chitosan (DD = 72.85%) to obtain biofilm of chitosan/DAC (CDAC), and characterization of biofilms. The crosslinked reaction was confirmed by FT-IR analysis that showed the spectrum of Schiff base C=N group at 1651 cm–1. Tensile strength increased gradually when the NaIO4 concentration used was 0.2–0.6 mg/mL, but after those concentrations, the tensile strength slightly decreased. The morphology analysis showed that CDAC had smoother morphology than DAC, which was shown rough and showed some particle indicated the presence of unreacted cellulose. CDAC biofilms that prepared with 1.0 mg/mL NaIO4 showed the greatest antibacterial activity.

scholarly journals NOVEL COMPOSITE OF SILVER-BACTERIAL CELLULOSE (Ag-BC) FROM SIWALAN SAP (Borassus flabellifer) AND ITS ANTIBACTERIAL ACTIVITY

2020 ◽  
Vol 83 (1) ◽  
pp. 19-25
Author(s):  
Suntini Suntini ◽  
Anastasia Wheni Indrianingsih ◽  
Harjono Harjono
Keyword(s):  
Antibacterial Activity ◽  
Bacterial Cellulose ◽  
Antibacterial Properties ◽  
Reduction Process ◽  
Diffusion Method ◽  
Natural Material ◽  
Gram Positive Bacteria ◽  
Antibacterial Assay ◽  
Natural Composite

Recently, a wound healing from natural composite with excellent properties is in a high demand. In this study, a novel composite of bacterial cellulose made from Siwalan sap (Borassus flabellifer) was achieved. Siwalan is a common plant in Java Island of Indonesia and the application is very limited for beverage only. This study aims to determine the effect of the AgNO3/NaBH4 concentration ratio in the development of Ag-BC composites and its antibacterial properties from Siwalan sap. Ag-BC composites were prepared by impregnating the silver solution into the BC matrix through the reduction process with NaBH4. Characterization of Ag-BC composites conducted using Fourier Transform Infrared Spectroscopy (FTIR), Scanning Electron Microscopy (SEM), and Energy Dispersive X-ray (EDX). Antibacterial assay was performed using disc diffusion method against Salmonella typhimurium (Gram-negative) and Staphylococcus aureus (Gram-positive) bacteria. The characterization of Ag-BC composite shows the nanostructure of BC with a length of fiber around 35-60 nm in width. The SEM-EDX micrograph showed that silver particles were impregnated into the BC matrix. Antibacterial activity test results showed that the Ag-BC composite had the ability to inhibit the bacteria S. typhimurium and S. aureus with good inhibition. This result showed the potential application of Ag-BC composite from Siwalan plant as a natural material for medical and pharmaceutical purpose, especially as an antibacterial agent.

scholarly journals Antibacterial Activity and Drug Loading of Moxifloxacin-Loaded Poly(Vinyl Alcohol)/Chitosan Electrospun Nanofibers

2021 ◽  
Vol 8 ◽  
Author(s):  
Qi Liu ◽  
Wen-Chong Ouyang ◽  
Xiu-Hong Zhou ◽  
Tao Jin ◽  
Zheng-Wei Wu
Keyword(s):  
Antibacterial Activity ◽  
Vinyl Alcohol ◽  
Composite Nanofibers ◽  
Drug Loading ◽  
Antibacterial Properties ◽  
Electrospun Nanofibers ◽  
Poly Vinyl Alcohol ◽  
X Ray Diffraction ◽  
Blank Group

In this study, nanofibers with different ratios of poly(vinyl alcohol) and chitosan incorporated with moxifloxacin hydrochloride (MH/PVA/CS) were fabricated through the blending electrospinning, and the morphological features were tested using scanning electron microscopy (SEM). Further characterization of the new nanofiber was accomplished by Thermogravimetric analysis (TG), X-ray diffraction (XRD) and Fourier-transform infrared spectroscopy (FTIR). Antibacterial activity of the MH-loaded nanofibers at different drug loading were tested and compared with the blank group. Experimental results show that the MH/PVA/CS nanofibers exhibited the good antibacterial properties against Staphylococcus aureus and Pseudomonas aeruginosa due to the MH incorporation. Compared with blank nanofibers, MH/PVA/CS nanofibers have significantly better antibacterial properties, and different proportions of PVA and CS have a certain effect on the antibacterial activity of nanofibers. The conclusions in this paper show that MH/PVA/CS composite nanofibers may have great potential in antibacterial materials.

scholarly journals Synthesis and Characterization of Selenium Nanoparticles-Lysozyme Nanohybrid System with Synergistic Antibacterial Properties

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Mahsa Vahdati ◽  
Tahereh Tohidi Moghadam
Keyword(s):  
Antibacterial Activity ◽  
Bacterial Growth ◽  
Biomedical Applications ◽  
Antibacterial Properties ◽  
Complex Form ◽  
Vital Role ◽  
Selenium Nanoparticles ◽  
Low Concentrations ◽  
Very High

AbstractIn the light of promising potency of selenium nanoparticles in biomedical applications, this is the first study to report the synergistic antibacterial activity of these nanoparticles and lysozyme. The nanohybrid system was prepared with various concentrations of each component. Resistance of Escherichia coli and Staphylococcus aureus was compared in the presence of individual Nano and Bio counterparts as well as the nanohybrid system. Upon interaction of SeNPs with Lysozyme, the nanohybrid system efficiently enhanced the antibacterial activity compared to the protein. Therefore, SeNPs play an important role in inhibition of bacterial growth at very low concentrations of protein; whereas very high amount of the protein is required to inhibit bacterial growth individually. On the other hand, lysozyme has also played a vital role in antibacterial property of SeNPs, inducing 100% inhibition at very low concentration of each component. Hence, presence of both nano and bio counterparts induced vital interplay in the Nanohybrid system. The aged samples also presented good stability of SeNPs both as the intact and complex form. Results of this effort highlight design of nanohybrid systems with synergistic antibacterial properties to overcome the emerging antibiotic resistance as well as to define fruitful applications in biomedicine and food safety.

scholarly journals Extraction and Characterization of Natural Dye from Green Walnut Shells and Its Use in Dyeing Polyamide: Focus on Antibacterial Properties

2013 ◽  
Vol 2013 ◽  
pp. 1-9 ◽  
Author(s):  
Mohammad Mirjalili ◽  
Loghman Karimi
Keyword(s):  
Antibacterial Activity ◽  
Antibacterial Properties ◽  
Natural Dye ◽  
Cupric Sulfate ◽  
Walnut Shells ◽  
Soxhlet Apparatus ◽  
Dyed Fabrics ◽  
Potassium Aluminum Sulfate ◽  
Layer Chromatography

Extraction of dyes from walnut using Soxhlet apparatus has been studied. The color components extracted and isolated from walnut shells were characterized by column chromatography, thin layer chromatography (TLC), nuclear magnetic resonance (NMR), mass spectroscopy (MS), and infrared (IR) techniques. Natural dye extract obtained from the walnut was used in dyeing polyamide fabrics with different mordants. The dyed fabrics were evaluated for antibacterial activity against pathogenic strains of Gram-positive (Staphylococcus aureus) and Gram-negative (Escherichia coli) bacteria. As such, the relationship between antibacterial activity and dye concentration is investigated. Durability of antibacterial activity to laundering is also discussed. Results indicate that the polyamide dyed with walnut displayed excellent antibacterial activity in the presence of ferric sulfate, cupric sulfate, and potassium aluminum sulfate and exhibited good and durable fastness properties.

scholarly journals Preparation and characterization of Copper Chitosan Nanocomposites with Antibacterial Activity for Applications in Tissue Engineering

2017 ◽  
Author(s):  
◽  
K. Maldonado-Lara
Keyword(s):  
Tissue Engineering ◽  
Antibacterial Activity ◽  
Potential Application ◽  
Antibacterial Properties ◽  
Hydroxyl Groups ◽  
Liquid Media ◽  
Solution Blending ◽  
Nanoparticles Dispersion ◽  
Bacterial Penetration

The Present work describes the preparation of nanocomposites based on chitosan (QS)/copper nanoparticles (nCu) with antibacterial properties and potential application in tissue engineering. For this purpose, nanocomposites were prepared by solution blending with ultrasound assisted, aiming to increase the nanoparticles dispersion in the biopolymer. FTIR analyses demonstrates that nCu supported in QS increase their interaction of nanoparticles with amine/hydroxyl groups of QS molecule. UV-Vis analyses demonstrates that QS/nCu nanocomposites have an absorption signal associated with the presence of nanoparticles and the possible Cu2+ ions release in liquid media. AFM analyses shown that hydrated QS form a mesh with micro pores, improving the bacterial penetration and the direct contact with nCu. This behavior was corroborated by antibacterial assays, where QS/nCu nanocomposites shown an antibacterial activity higher than 90% between 90-180 minutes of interaction. Our results suggest that is possible to obtain combined antibacterial/biocompatible nanomaterials with potential application in tissue engineering.

scholarly journals The Effect of Ag Content of the Chitosan-Silver Nanoparticle Composite Material on the Structure and Antibacterial Activity

2013 ◽  
Vol 2013 ◽  
pp. 1-6 ◽  
Author(s):  
Solmaz Akmaz ◽  
Esra Dilaver Adıgüzel ◽  
Muzaffer Yasar ◽  
Oray Erguven
Keyword(s):  
Antibacterial Activity ◽  
Composite Material ◽  
Composite Materials ◽  
Silver Nanoparticle ◽  
Antibacterial Properties ◽  
Chitosan Solution ◽  
X Ray Diffraction ◽  
Properties And Characterization ◽  
Transmission Electron

The aim of this study is to investigate the antibacterial properties and characterization of chitosan-silver nanoparticle composite materials. Chitosan-silver nanoparticle composite material was synthesized by adding AgNO3and NaOH solutions to chitosan solution at 95°C. Different concentrations (0,02 M, 0,04 M, and 0,06 M) of AgNO3were used for synthesis. Chitosan-silver nanoparticle composite materials were characterized by Transmission electron microscopy (TEM), X-ray diffraction (XRD), ultraviolet (UV) spectrophotometer, and Fourier transform infrared (FTIR) spectrometer techniques.Escherichia coli,Acinetobacter baumannii,Staphylococcus aureus,Enterococcus faecalis,Pseudomonas aeruginosa, andStreptococcus pneumoniaewere used to test the bactericidal efficiency of synthesized chitosan-Ag nanoparticle composite materials. The biological activity was determined by the minimum bacterial concentration (MBC) of the materials. Antibacterial effect of chitosan-silver nanoparticle materials was increased by increasing Ag amount of the composite materials. The presence of small amount of metal nanoparticles in the composite was enough to significantly enhance antibacterial activity as compared with pure chitosan.

scholarly journals Liver Tissue Regeneration using Nano Silver impregnated Sodium Alginate/PVA Composite Nanofibres

2020 ◽  
Vol 2 (1) ◽  
pp. 16-21 ◽  
Author(s):  
Manikandan G ◽  
Yuvashree M ◽  
Sangeetha A ◽  
Bhuvana K P ◽  
Sanjay K Nayak
Keyword(s):  
Antibacterial Activity ◽  
Sodium Alginate ◽  
Ag Nanoparticles ◽  
Liver Tissue ◽  
Average Particle Size ◽  
Antibacterial Properties ◽  
Poly Vinyl Alcohol ◽  
Average Particle ◽  
Ag Nps

Liver regeneration is a highly organized tissue regrowth process and is the most important reaction of the injured liver. The present study endeavors towards the preparation and characterization of nanoporous Sodium Alginate (SA)/ Poly Vinyl Alcohol (PVA) composite, nanofibrous scaffolds coated with silver (Ag) nanoparticles for hepatocellular regeneration.  Chitosan based Silver nanoparticles possess high antibacterial activity has been preferred in the scaffold preparation to improve the antibacterial properties. The structural characterization of Ag Nanoparticles revealed the amorphous nature with an average particle size of 300 nm. Nanofibres (Scaffolds) were prepared by electrospinning SA/PVA solution at a voltage of 18-25 kV and Ag NPs were coated on it for antibacterial activity. Invitro studies denoted the growth of nitro compounds, amides and collagen which are the major constituents of liver tissue.

scholarly journals Preparation and characterization of Copper Chitosan Nanocomposites with Antibacterial Activity for Applications in Tissue Engineering

2017 ◽  
Author(s):  
◽  
K. Maldonado-Lara
Keyword(s):  
Tissue Engineering ◽  
Antibacterial Activity ◽  
Potential Application ◽  
Antibacterial Properties ◽  
Hydroxyl Groups ◽  
Liquid Media ◽  
Solution Blending ◽  
Nanoparticles Dispersion ◽  
Bacterial Penetration

The Present work describes the preparation of nanocomposites based on chitosan (QS)/copper nanoparticles (nCu) with antibacterial properties and potential application in tissue engineering. For this purpose, nanocomposites were prepared by solution blending with ultrasound assisted, aiming to increase the nanoparticles dispersion in the biopolymer. FTIR analyses demonstrates that nCu supported in QS increase their interaction of nanoparticles with amine/hydroxyl groups of QS molecule. UV-Vis analyses demonstrates that QS/nCu nanocomposites have an absorption signal associated with the presence of nanoparticles and the possible Cu2+ ions release in liquid media. AFM analyses shown that hydrated QS form a mesh with micro pores, improving the bacterial penetration and the direct contact with nCu. This behavior was corroborated by antibacterial assays, where QS/nCu nanocomposites shown an antibacterial activity higher than 90% between 90-180 minutes of interaction. Our results suggest that is possible to obtain combined antibacterial/biocompatible nanomaterials with potential application in tissue engineering.

scholarly journals STUDIES ON BIOSURFACTANT PRODUCED USING Exiguobacterium profundum

2020 ◽  
Vol 2 (2) ◽  
Author(s):  
Nur Asni Setiani ◽  
Welly Octaviyani ◽  
Syarif Hamdani ◽  
Irma Mardiah
Keyword(s):  
Escherichia Coli ◽  
Antibacterial Activity ◽  
Antibacterial Properties ◽  
Pharmaceutical Preparations ◽  
Initial Screening ◽  
Gram Negative ◽  
Emulsification Index ◽  
Exiguobacterium Profundum ◽  
Layer Chromatography

Background: The manufacture of pharmaceutical preparations generally adds surfactants. Microbial biosurfactants can be an alternative because biodegradable and have antibacterial properties.   Objective: This study aimed to examine the biosurfactant activity of Exiguobacterium profundum.Methods: Hemolysis and spreading oil tests were performed as an initial screening. Biosurfactant production was carried out by growing bacteria on oil-enriched media with shaker system for 7 days. Biosurfactant activity can be seen from the emulsification index, while the characterization of biosurfactant were used thin layer chromatography and antibacterial qualitative testing.Results: Exiguobacterium profundum could spread the oil layer and form micelles. The emulsification index on days 0, 1, 3, 5, and 7 showed percentage in sequence 44.83%, 48.28%, 48.28%, 40%, and 43.75%. The result of TLC showed lipopeptide group which is marked with red stain with ninhydrin appearance. Antibacterial testing using Escherichia coli showed the formation of clear zones around the disk paper.Conclusion: The biosurfactant produced by Exigoubacterium profundum can be classified into lipopeptide group which has antibacterial activity against gram-negative.

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