Synthesis and Characterization of Selenium Nanoparticles-Lysozyme Nanohybrid System with Synergistic Antibacterial Properties

AbstractIn the light of promising potency of selenium nanoparticles in biomedical applications, this is the first study to report the synergistic antibacterial activity of these nanoparticles and lysozyme. The nanohybrid system was prepared with various concentrations of each component. Resistance of Escherichia coli and Staphylococcus aureus was compared in the presence of individual Nano and Bio counterparts as well as the nanohybrid system. Upon interaction of SeNPs with Lysozyme, the nanohybrid system efficiently enhanced the antibacterial activity compared to the protein. Therefore, SeNPs play an important role in inhibition of bacterial growth at very low concentrations of protein; whereas very high amount of the protein is required to inhibit bacterial growth individually. On the other hand, lysozyme has also played a vital role in antibacterial property of SeNPs, inducing 100% inhibition at very low concentration of each component. Hence, presence of both nano and bio counterparts induced vital interplay in the Nanohybrid system. The aged samples also presented good stability of SeNPs both as the intact and complex form. Results of this effort highlight design of nanohybrid systems with synergistic antibacterial properties to overcome the emerging antibiotic resistance as well as to define fruitful applications in biomedicine and food safety.

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Antibacterial Properties of Biofilm Schiff Base Derived from Dialdehyde Cellulose and Chitosan

Indonesian Journal of Chemistry ◽

10.22146/ijc.34721 ◽

2019 ◽

Vol 19 (2) ◽

pp. 405

Author(s):

Agung Pratama ◽

Firman Sebayang ◽

Rumondang Bulan Nasution

Keyword(s):

Tensile Strength ◽

Antibacterial Activity ◽

Schiff Base ◽

Antibacterial Properties ◽

Oxidized Cellulose ◽

Natural Polymers ◽

Dialdehyde Cellulose ◽

Ft Ir ◽

Ir Analysis

Cellulose and chitosan are natural polymers that have been used as biocomposite. The aim of this research is to obtain biofilms from chitosan and oxidized cellulose crosslinks. This research is divided into three steps, i.e., isolation of cellulose from oil palm trunk and oxidation of cellulose using NaIO4 (0.2; 0.4; 0.6; 0.8; 1.0 mg/mL) to obtain dialdehyde cellulose (DAC), crosslink of oxidized cellulose with chitosan (DD = 72.85%) to obtain biofilm of chitosan/DAC (CDAC), and characterization of biofilms. The crosslinked reaction was confirmed by FT-IR analysis that showed the spectrum of Schiff base C=N group at 1651 cm–1. Tensile strength increased gradually when the NaIO4 concentration used was 0.2–0.6 mg/mL, but after those concentrations, the tensile strength slightly decreased. The morphology analysis showed that CDAC had smoother morphology than DAC, which was shown rough and showed some particle indicated the presence of unreacted cellulose. CDAC biofilms that prepared with 1.0 mg/mL NaIO4 showed the greatest antibacterial activity.

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NOVEL COMPOSITE OF SILVER-BACTERIAL CELLULOSE (Ag-BC) FROM SIWALAN SAP (Borassus flabellifer) AND ITS ANTIBACTERIAL ACTIVITY

Jurnal Teknologi ◽

10.11113/jurnalteknologi.v83.14363 ◽

2020 ◽

Vol 83 (1) ◽

pp. 19-25

Author(s):

Suntini Suntini ◽

Anastasia Wheni Indrianingsih ◽

Harjono Harjono

Keyword(s):

Antibacterial Activity ◽

Bacterial Cellulose ◽

Antibacterial Properties ◽

Reduction Process ◽

Diffusion Method ◽

Natural Material ◽

Gram Positive Bacteria ◽

Antibacterial Assay ◽

Natural Composite

Recently, a wound healing from natural composite with excellent properties is in a high demand. In this study, a novel composite of bacterial cellulose made from Siwalan sap (Borassus flabellifer) was achieved. Siwalan is a common plant in Java Island of Indonesia and the application is very limited for beverage only. This study aims to determine the effect of the AgNO3/NaBH4 concentration ratio in the development of Ag-BC composites and its antibacterial properties from Siwalan sap. Ag-BC composites were prepared by impregnating the silver solution into the BC matrix through the reduction process with NaBH4. Characterization of Ag-BC composites conducted using Fourier Transform Infrared Spectroscopy (FTIR), Scanning Electron Microscopy (SEM), and Energy Dispersive X-ray (EDX). Antibacterial assay was performed using disc diffusion method against Salmonella typhimurium (Gram-negative) and Staphylococcus aureus (Gram-positive) bacteria. The characterization of Ag-BC composite shows the nanostructure of BC with a length of fiber around 35-60 nm in width. The SEM-EDX micrograph showed that silver particles were impregnated into the BC matrix. Antibacterial activity test results showed that the Ag-BC composite had the ability to inhibit the bacteria S. typhimurium and S. aureus with good inhibition. This result showed the potential application of Ag-BC composite from Siwalan plant as a natural material for medical and pharmaceutical purpose, especially as an antibacterial agent.

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Synthesis characterization and antibacterial studies of 4-aminoantipyrine schiff’s bases

International Journal of Applied Pharmaceutical Sciences and Research ◽

10.21477/ijapsr.v2i1.6908 ◽

2017 ◽

Vol 2 (01) ◽

pp. 8-14 ◽

Cited By ~ 1

Author(s):

K Sunand ◽

K Vinay Kumar ◽

K Ashwini ◽

P Suresh Kumar ◽

S Vishnu ◽

...

Keyword(s):

Antibacterial Activity ◽

Schiff Bases ◽

Vital Role ◽

Spectroscopic Techniques ◽

Schiff’S Bases ◽

Pharmacological Activities ◽

Schiff's Bases ◽

Ir Spectroscopic

Aim: To synthesize and evaluate 4-aminoantipyrine related schiff’s bases as antibacterial agents. Objective: To synthesize, purify, characterize and evaluate 4-aminoantipyrine. Method: Schiff bases derived from 4-aminoantipyrine play a vital role in biological and pharmacological activities. Knowing the importance of 4-aminoatipyrine schiff bases and their analogues wide varieties of bioactivities like analgesic, antiviral, antipyretic, anti-rheumatic, antimicrobial and anti-inflammatory activities have been widely studied. 4-aminoantipyrine compounds C1 (anisaldehyde), C2 (p-hydroxybenzaldehyde) and C3(vanillin) were prepared by condensation between 4-amino antipyrine and substituted aromatic benzaldehydes. The products were purified by recrystallization by using ethanol, characterized by IR spectroscopy. The N-H stretching in 4-aminoantipyrine is shown at 3430 cm-1 and -3325 cm-1. The -HC=N- stretching is observed in the range of 1508-1504 cm-1 The –OCH3 stretching is found at 1888 cm-1. 4-amino antipyrine related schiff’s bases evaluated their activity as antimicrobials in-vitro by spread plate method against E.coli. Schiff bases have potent antibacterial activity with gram negative bacteria E.coli. Results: Synthesis and characterization of a schiff bases derived from substituted benzaldehydes and 4-aminoantipyrine was evaluated and characterized with the IR spectroscopic techniques and schiff bases have shown potent antibacterial activity against E.Coli.

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Antibacterial Activity and Drug Loading of Moxifloxacin-Loaded Poly(Vinyl Alcohol)/Chitosan Electrospun Nanofibers

Frontiers in Materials ◽

10.3389/fmats.2021.643428 ◽

2021 ◽

Vol 8 ◽

Author(s):

Qi Liu ◽

Wen-Chong Ouyang ◽

Xiu-Hong Zhou ◽

Tao Jin ◽

Zheng-Wei Wu

Keyword(s):

Antibacterial Activity ◽

Vinyl Alcohol ◽

Composite Nanofibers ◽

Drug Loading ◽

Antibacterial Properties ◽

Electrospun Nanofibers ◽

Poly Vinyl Alcohol ◽

X Ray Diffraction ◽

Blank Group

In this study, nanofibers with different ratios of poly(vinyl alcohol) and chitosan incorporated with moxifloxacin hydrochloride (MH/PVA/CS) were fabricated through the blending electrospinning, and the morphological features were tested using scanning electron microscopy (SEM). Further characterization of the new nanofiber was accomplished by Thermogravimetric analysis (TG), X-ray diffraction (XRD) and Fourier-transform infrared spectroscopy (FTIR). Antibacterial activity of the MH-loaded nanofibers at different drug loading were tested and compared with the blank group. Experimental results show that the MH/PVA/CS nanofibers exhibited the good antibacterial properties against Staphylococcus aureus and Pseudomonas aeruginosa due to the MH incorporation. Compared with blank nanofibers, MH/PVA/CS nanofibers have significantly better antibacterial properties, and different proportions of PVA and CS have a certain effect on the antibacterial activity of nanofibers. The conclusions in this paper show that MH/PVA/CS composite nanofibers may have great potential in antibacterial materials.

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Yeast actin with a subdomain 4 mutation (A204C) exhibits increased pointed-end critical concentration

Biochemistry and Cell Biology ◽

10.1139/o07-047 ◽

2007 ◽

Vol 85 (3) ◽

pp. 319-325 ◽

Cited By ~ 6

Author(s):

David J. Teal ◽

John F. Dawson

Keyword(s):

Actin Polymerization ◽

Critical Concentration ◽

Structural Studies ◽

Wild Type ◽

Low Concentrations ◽

Engineering Strategy ◽

High Concentrations ◽

Pointed End ◽

Very High

Characterizing mutants of actin that do not polymerize will advance our understanding of the mechanism of actin polymerization and will be invaluable for the production of short F-actin structures for structural studies. To circumvent the problem of expressing dominant lethal nonpolymerizing actin in yeast, we adopted a cysteine engineering strategy. Here we report the characterization of a mutant of yeast actin, AC-actin, possessing a single pointed-end mutation, A204C. Expression of this mutant in yeast results in actin-polymerization-deficient phenotypes. When copolymerized with wild-type actin, ATP–AC-actin is incorporated into filaments. ADP–AC-actin participates in the nucleation and elongation of wild-type filaments only at very high concentrations. At low concentrations, ADP–AC-actin appears to participate only in the nucleation of wild-type filaments, suggesting that Ala-204 is involved in modulating the critical concentration of the pointed end of actin.

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Antibacterial Properties among Different Concentration of Bioactive Glasses

Key Engineering Materials ◽

10.4028/www.scientific.net/kem.814.349 ◽

2019 ◽

Vol 814 ◽

pp. 349-353

Author(s):

Tanawan Wanitwisutchai ◽

Naruporn Monmaturapoj ◽

Ratchapin Srisatjaluk ◽

Kittitat Subannajui ◽

Niwat Anuwongnukroh ◽

...

Keyword(s):

Zinc Oxide ◽

Antibacterial Activity ◽

Ph Value ◽

Antibacterial Properties ◽

Brain Heart Infusion ◽

Bioactive Glasses ◽

Ph Changes ◽

Constant Ph ◽

Low Concentrations ◽

Zinc Oxide Nanowire

The objective of this study was to evaluate the antibacterial properties and pH changes of bioactive glasses and zinc oxide nanowire in different concentrations. Bioactive glasses (45S5 and 45S5F) were prepared in three concentrations of 10, 20, and 50 mg/ml and zinc oxide nanowire was prepared in 1 and 5 mg/ml concentrations. The materials were exposed to 500 ml brain heart infusion broth (BHI) with 1.5 x 107 of S.mutans and S.sanguinis separately. Antibacterial properties were tested indirectly by collecting 100 ml of each sample and transferred into a 96 well-plate. The optical density (OD) was evaluated using spectrophotometry at 630 nm at 24h and 48h. The pH changes were recorded. The data were statistically analyzed by Kruskal-Wallis tests. The result showed that the pH changes were significantly different in the Bioactive glass samples, while zinc oxide nanowire showed stable pH. Antibacterial activity against S.mutans was significant lower for 45S5 at 50 mg/ml, 45S5F and zinc oxide nanowire in all concentrations at 24 h. While in 48 h, 45S5, 45S5F and zinc oxide nanowire showed significant antibacterial activity in all concentration except 45S5F at 10 mg/ml. Antibacterial activity against S.sanguinis was significant for 45S5 and 45S5F at 20 and 50 mg/ml and zinc oxide nanowire in all concentration at 48h. It can be concluded that Bioactive glasses (45S5 and 45S5F) exhibited antibacterial properties and pH changes depending on its concentration, while zinc oxide nanowire exhibited antibacterial properties at low concentrations with a constant pH value.

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Quaternary Ammonium Polyethyleneimine: Antibacterial Activity

Journal of Nanomaterials ◽

10.1155/2010/826343 ◽

2010 ◽

Vol 2010 ◽

pp. 1-11 ◽

Cited By ~ 45

Author(s):

Ira Yudovin-Farber ◽

Jacob Golenser ◽

Nurit Beyth ◽

Ervin I. Weiss ◽

Abraham J. Domb

Keyword(s):

Antibacterial Activity ◽

Methyl Iodide ◽

Bacterial Growth ◽

Quaternary Ammonium ◽

Reductive Amination ◽

Antibacterial Properties ◽

Synthetic Methods ◽

Cytotoxicity Studies ◽

Pei Nanoparticles

Quaternary ammonium polyethyleneimine- (QA-PEI-) based nanoparticles were synthesized using two synthetic methods, reductive amination and N-alkylation. According to the first method, QA-PEI nanoparticles were synthesized by cross-linking with glutaraldehyde followed by reductive amination with octanal and further N-methylation with methyl iodide. The second method is based on crosslinking with dialkyl halide followed by N-alkylation with octyl halide and further N-methylation with methyl iodide. QA-PEI nanoparticles completely inhibited bacterial growth (>106bacteria), including both Gram-positive, that is,Staphylococcus aureusat 80 g/mL, and Gram-negative, that is,Escherichia coliat 320 g/mL. Activity analysis revealed that the degree of alkylation and N-methylation of the QA-PEI nanoparticles plays a significant role in antibacterial activity of the reagent. The most potent compound was octyl alkylated QA-PEI alkylated at 1 : 1 mole ratio (primary amine of PEI monomer units/alkylating agent). Also, cytotoxicity studies on MAT-LyLu and MBT cell lines were performed with QA-PEI nanoparticles. These findings confirm previous reports that polycations bearing quaternary ammonium moieties inhibit bacterial growthin vitroand have a potential use as additives in medical devices which need antibacterial properties.

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Extraction and Characterization of Natural Dye from Green Walnut Shells and Its Use in Dyeing Polyamide: Focus on Antibacterial Properties

Journal of Chemistry ◽

10.1155/2013/375352 ◽

2013 ◽

Vol 2013 ◽

pp. 1-9 ◽

Cited By ~ 25

Author(s):

Mohammad Mirjalili ◽

Loghman Karimi

Keyword(s):

Antibacterial Activity ◽

Antibacterial Properties ◽

Natural Dye ◽

Cupric Sulfate ◽

Walnut Shells ◽

Soxhlet Apparatus ◽

Dyed Fabrics ◽

Potassium Aluminum Sulfate ◽

Layer Chromatography

Extraction of dyes from walnut using Soxhlet apparatus has been studied. The color components extracted and isolated from walnut shells were characterized by column chromatography, thin layer chromatography (TLC), nuclear magnetic resonance (NMR), mass spectroscopy (MS), and infrared (IR) techniques. Natural dye extract obtained from the walnut was used in dyeing polyamide fabrics with different mordants. The dyed fabrics were evaluated for antibacterial activity against pathogenic strains of Gram-positive (Staphylococcus aureus) and Gram-negative (Escherichia coli) bacteria. As such, the relationship between antibacterial activity and dye concentration is investigated. Durability of antibacterial activity to laundering is also discussed. Results indicate that the polyamide dyed with walnut displayed excellent antibacterial activity in the presence of ferric sulfate, cupric sulfate, and potassium aluminum sulfate and exhibited good and durable fastness properties.

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Preparation and characterization of Copper Chitosan Nanocomposites with Antibacterial Activity for Applications in Tissue Engineering

10.17488/rmib.381.26 ◽

2017 ◽

Author(s):

◽

K. Maldonado-Lara

Keyword(s):

Tissue Engineering ◽

Antibacterial Activity ◽

Potential Application ◽

Antibacterial Properties ◽

Hydroxyl Groups ◽

Liquid Media ◽

Solution Blending ◽

Nanoparticles Dispersion ◽

Bacterial Penetration

The Present work describes the preparation of nanocomposites based on chitosan (QS)/copper nanoparticles (nCu) with antibacterial properties and potential application in tissue engineering. For this purpose, nanocomposites were prepared by solution blending with ultrasound assisted, aiming to increase the nanoparticles dispersion in the biopolymer. FTIR analyses demonstrates that nCu supported in QS increase their interaction of nanoparticles with amine/hydroxyl groups of QS molecule. UV-Vis analyses demonstrates that QS/nCu nanocomposites have an absorption signal associated with the presence of nanoparticles and the possible Cu2+ ions release in liquid media. AFM analyses shown that hydrated QS form a mesh with micro pores, improving the bacterial penetration and the direct contact with nCu. This behavior was corroborated by antibacterial assays, where QS/nCu nanocomposites shown an antibacterial activity higher than 90% between 90-180 minutes of interaction. Our results suggest that is possible to obtain combined antibacterial/biocompatible nanomaterials with potential application in tissue engineering.

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Simultaneous Sonochemical Coloration and Antibacterial Functionalization of Leather with Selenium Nanoparticles (SeNPs)

Polymers ◽

10.3390/polym14010074 ◽

2021 ◽

Vol 14 (1) ◽

pp. 74

Author(s):

Tarek Abou Elmaaty ◽

Khaled Sayed-Ahmed ◽

Radwan Mohamed Ali ◽

Kholoud El-Khodary ◽

Shereen A. Abdeldayem

Keyword(s):

Electron Microscopy ◽

Antibacterial Activity ◽

Antibacterial Properties ◽

Salmonella Typhi ◽

Point Of View ◽

Selenium Nanoparticles ◽

Footwear Industry ◽

Transmission Electron ◽

Vis Spectroscopy ◽

Low Cytotoxicity

The development of antibacterial coatings for footwear components is of great interest both from an industry and consumer point of view. In this work, the leather material was developed taking advantage of the intrinsic antibacterial activity and coloring ability of selenium nanoparticles (SeNPs). The SeNPs were synthesized and implemented into the leather surface by using ultrasonic techniques to obtain simultaneous coloring and functionalization. The formation of SeNPs in the solutions was evaluated using UV/Vis spectroscopy and the morphology of the NPs was determined by transmission electron microscopy (TEM). The treated leather material (leather/SeNPs) was characterized by scanning electron microscopy (SEM) and energy dispersive X-ray spectroscopy (EDX). The effects of SeNPs on the coloration and antibacterial properties of the leather material were evaluated. The results revealed that the NPs were mostly spherical in shape, regularly distributed, and closely anchored to the leather surface. The particle size distribution of SeNPs at concentrations of 25 mM and 50 mM was in the range of 36–77 nm and 41–149 nm, respectively. It was observed that leather/SeNPs exhibited a higher depth of shade compared to untreated ones, as well as excellent fastness properties. The results showed that leather/SeNPs can significantly enhance the antibacterial activity against model of bacteria, including Gram-positive bacteria (Bacillus cereus) and Gram-negative bacteria (Pseudomonas aeruginosa, Salmonella typhi and Escherichia coli). Moreover, the resulting leather exhibited low cytotoxicity against HFB4 cell lines. This achievement should be quite appealing to the footwear industry as a way to prevent the spread of bacterial infection promoted by humidity, poor breathability and temperature which promote the expansion of the microflora of the skin.

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