Effect of the Surface Energy on the Biomineralization Behavior and Cell Compatibility of Carbonated Hydroxyapatite

2009 ◽  
Vol 610-613 ◽  
pp. 1203-1207
Author(s):  
Qing Xia Zhu ◽  
Jian Qing Wu ◽  
Qing Feng ◽  
He Ping Wang
Keyword(s):  
Surface Energy ◽  
Cell Attachment ◽  
Polar Component ◽  
Carbonate Apatite ◽  
Carbonated Hydroxyapatite ◽  
Cell Compatibility ◽  
Biomineralization Process ◽  
Wet Chemical ◽  
Sbf Solution

Carbonated hydroxyapatite (CHA) and hydroxyapatite (HA) were prepared by the wet chemical method. The contact angle was measured to calculate the surface energy. The biomineralization process in SBF solution of HA and CHA were investigated in vitro. The cell attachment and proliferation behavior of CHA and HA were compared by the cell culture experiments. The results show that the polar component of surface energy of B-type CHA is higher than that of HA. Osteoblastic cells attach and proliferate very well on the surface, which indicates the excellent cell compatibility. The CHA have high bioactivity owing to rapid formation of hydroxyl-carbonate-apatite (HCA) mineralized layer on the biomaterial’s surface in SBF.

Surface Modification of Calcium Hydroxyfluor Carbonate Apatites by Bisphosphonates

2005 ◽  
Vol 284-286 ◽  
pp. 357-360 ◽  
Author(s):  
Luis M. Rodríguez-Lorenzo ◽  
Blanca Vázquez ◽  
Julio San Román
Keyword(s):  
Thermal Analysis ◽  
Contact Angle ◽  
Weight Loss ◽  
Surface Modification ◽  
Bone Resorption ◽  
Ir Spectroscopy ◽  
Surface Energy ◽  
Cellular Interaction ◽  
Polar Component ◽  
Carbonate Apatite

Hydroxyapatite has been frequently described as an osteoconductive but not osteoinductive material based on failure to observe bone formation in nonbony sites. Bisphosphonates (BPs) are stable pyrophosphate analogs, that enhance the proliferation, differentiation and bone forming activity of osteoblasts and are potent inhibitors of bone resorption. In this paper, the modification of a calcium hydroxyfluor carbonate apatite with sodium alendronate and (4-(aminomethyl)benzene)bisphosphonic acid is described. The surface modification is carried out by refluxing the apatite in a bisphosphonate acetone solution. Modified particles are characterized by thermal analysis, ATR-IR spectroscopy and contact angle between other techniques. A weight loss between 150 and 500°C can be observed for the modified apatites. IR spectra show the appearance of bisphosphonate bands on modified powders. The surface energy of the modified apatite is reduced up to a 74% from the total apatite value after the alendronate surface modification with a decrease of 58% of the polar component main responsible of the cellular interaction of biomaterials.

Bone Marrow Cell Response on Carbonate Apatite/PCL-Coated α-Tricalcium Phosphate Foam

2013 ◽  
Vol 858 ◽  
pp. 7-12
Author(s):  
Thi Bang Le ◽  
Xing Ling Shi ◽  
Ishikawa Kunio ◽  
Radzali Othman
Keyword(s):  
Bone Marrow ◽  
Tricalcium Phosphate ◽  
Bone Marrow Cells ◽  
Cell Attachment ◽  
Marrow Cell ◽  
Research Work ◽  
Biological Properties ◽  
Carbonate Apatite ◽  
Vitro Effect

The aim of this research work was to investigate in vitro effect of the carbonate apatite/poly (ε-caprolactone) (CO3Ap/PCL) on α-tricalcium phosphate (α-TCP) foam was produced by sintering CaCO3and CaHPO42H2O at 1500°C for 5 h. It was then coated with carbonate apatite (CO3Ap)/Poly-ε-caprolactone (PCL) (wt/wt=1/3) to improve both mechanical and biological properties. The initial cell attachment and proliferation of the bone marrow cells were carried out on the α-TCP and CO3Ap/PCL-coated α-TCP foams. The cell proliferation was calculated by AlamarBlue assay. The cells were able to migrate and proliferate well on both α-TCP and CO3Ap/PCL-coated α-TCP foams indicating an excellent biocompatibility. The incorporation of CO3Ap on the coating layer improved cellular attachment and accelerated proliferation. Thus, CO3Ap/PCL-coated α-TCP foam might be a promising candidate as implant material.

Sol Gel Synthesis and In Vitro Evaluation of Apatite Forming Ability of Silica-Based Composite Glass in SBF

2015 ◽  
Vol 660 ◽  
pp. 125-131 ◽  
Author(s):  
S.A. Syed Nuzul Fadzli ◽  
S. Roslinda ◽  
Firuz Zainuddin
Keyword(s):  
Crystalline Structure ◽  
Sol Gel ◽  
Xrd Analysis ◽  
Carbonate Apatite ◽  
Ftir Analysis ◽  
Apatite Structure ◽  
Composite Glass ◽  
Sbf Solution ◽  
Sol Gel Synthesis

In this study, xerogel glass based on SiO-CaO-PO4 was synthesized by a low temperature acid catalysed sol-gel route. The in vitro evaluation of apatite forming ability for the glass was conducted in simulated body fluid (SBF) solution as the glasses were immersed for duration of 1, 7, 24 hours and 7 days. The XRD analysis showed that the glass formed semi-crystalline structure when sintered at 1000oC and consisted of Ca2O7P2 and Ca2O4Si phases. Image captured using FESEM showed the apatite-like structures were eventually formed on the glass top surface in small numbers after the glass immersed in SBF for only an hour. The numbers of the apatite structures were continuously grown with the increase period of immersion time. The apatite structure mostly covered on top of the glass surface after 24 hours of immersion and continuously growth into bone-like apatite structure when immersed for 7 days in the SBF. The apatite layer formed on the surface of the glass was confirmed as crystalline structure of hydroxyl-carbonate-apatite (HCA) as revealed by the complimentary results of EDS, XRD and FTIR analysis.

scholarly journals Development of a Highly Proliferated Bilayer Coating on 316L Stainless Steel Implants

Polymers ◽  
2020 ◽  
Vol 12 (5) ◽  
pp. 1022 ◽  
Author(s):  
Fatemeh Khosravi ◽  
Saied Nouri Khorasani ◽  
Shahla Khalili ◽  
Rasoul Esmaeely Neisiany ◽  
Erfan Rezvani Ghomi ◽  
...  
Keyword(s):  
Stainless Steel ◽  
Cell Attachment ◽  
Electrospun Nanofibers ◽  
Water Contact ◽  
Bilayer Coating ◽  
316 L Stainless Steel ◽  
Sbf Solution ◽  
Vitro Analysis ◽  
Cell Growth And Proliferation

In this research, a bilayer coating has been applied on the surface of 316 L stainless steel (316LSS) to provide highly proliferated metallic implants for bone regeneration. The first layer was prepared using electrophoretic deposition of graphene oxide (GO), while the top layer was coated utilizing electrospinning of poly (ε-caprolactone) (PCL)/gelatin (Ge)/forsterite solutions. The morphology, porosity, wettability, biodegradability, bioactivity, cell attachment and cell viability of the prepared coatings were evaluated. The Field Emission Scanning Electron Microscopy (FESEM) results revealed the formation of uniform, continuous, and bead-free nanofibers. The Energy Dispersive X-ray (EDS) results confirmed well-distributed forsterite nanoparticles in the structure of the top coating. The porosity of the electrospun nanofibers was found to be above 70%. The water contact angle measurements indicated an improvement in the wettability of the coating by increasing the amount of nanoparticles. Furthermore, the electrospun nanofibers containing 1 and 3 wt.% of forsterite nanoparticles showed significant bioactivity after soaking in the simulated body fluid (SBF) solution for 21 days. In addition, to investigate the in vitro analysis, the MG-63 cells were cultured on the PCL/Ge/forsterite and GO-PCL/Ge/forsterite coatings. The results confirmed an excellent cell adhesion along with considerable cell growth and proliferation. It should be also noted that the existence of the forsterite nanoparticles and the GO layer substantially enhanced the cell proliferation of the coatings.

scholarly journals Zn- or Cu-containing CaP-Based Coatings Formed by Micro-Arc Oxidation on Titanium and Ti-40Nb Alloy: Part II—Wettability and Biological Performance

Materials ◽  
2020 ◽  
Vol 13 (19) ◽  
pp. 4366
Author(s):  
Ekaterina G. Komarova ◽  
Yurii P. Sharkeev ◽  
Mariya B. Sedelnikova ◽  
Oleg Prymak ◽  
Matthias Epple ◽  
...  
Keyword(s):  
Surface Energy ◽  
Pure Titanium ◽  
Multipotent Mesenchymal Stromal Cells ◽  
Contact Angles ◽  
Polar Component ◽  
Micro Arc Oxidation ◽  
Main Component ◽  
Biological Performance

This work describes the wettability and biological performance of Zn- and Cu-containing CaP-based coatings prepared by micro-arc oxidation on pure titanium (Ti) and novel Ti-40Nb alloy. Good hydrophilic properties of all the coatings were demonstrated by the low contact angles with liquids, not exceeding 45°. An increase in the applied voltage led to an increase of the coating roughness and porosity, thereby reducing the contact angles to 6° with water and to 17° with glycerol. The free surface energy of 75 ± 3 mJ/m2 for all the coatings were determined. Polar component was calculated as the main component of surface energy, caused by the presence of strong polar PO43− and OH− bonds. In vitro studies showed that low Cu and Zn amounts (~0.4 at.%) in the coatings promoted high motility of human adipose-derived multipotent mesenchymal stromal cells (hAMMSC) on the implant/cell interface and subsequent cell ability to differentiate into osteoblasts. In vivo study demonstrated 100% ectopic bone formation only on the surface of the CaP coating on Ti. The Zn- and Cu-containing CaP coatings on both substrates and the CaP coating on the Ti-40Nb alloy slightly decreased the incidence of ectopic osteogenesis down to 67%. The MAO coatings showed antibacterial efficacy against Staphylococcus aureus and can be arranged as follows: Zn-CaP/Ti > Cu-CaP/TiNb, Zn-CaP/TiNb > Cu-CaP/Ti.

Anti-Sticking Properties of PVD CrWNx, CrOx and ZrOx Coatings on Medical Electrode Application

2010 ◽  
Vol 297-301 ◽  
pp. 656-663
Author(s):  
Y.L. Hsu ◽  
C.H. Lee ◽  
S.M. Chiu ◽  
Y.C. Sung ◽  
K.Y. Yang ◽  
...  
Keyword(s):  
Contact Angle ◽  
Surface Energy ◽  
High Surface ◽  
Contact Angle Measurement ◽  
Angle Measurement ◽  
Polar Component ◽  
In Vitro Test ◽  
Unbalanced Magnetron Sputtering ◽  
Unbalanced Magnetron

The side effect of electrosurgery includes tissue charring, smoke generation and the adhesion of tissue to electrodes. These effects prolong surgery and interfere with effective coagulation. In this paper, CrWNx, CrOx and ZrOx coating were prepared by an unbalanced magnetron sputtering. The microstructure of films was characterized using XRD, XPS, TEM and AFM. The hydrophobicity and surface energy of coatings were calculated by contact angle measurement and Wu harmonic mean approach. Anti-sticking in vitro test was performed by monopolar electrosurgery using pork liver tissue. The hardness of CrWNx , ZrOx and CrOx coatings were 44 GPa, 26.3 GPa and 20.7 GPa, respectively. The CrOx coating had the lowest surface energy 33.5 mN/m and the highest contact angle of water as high as 103°. The high surface O-H bonds density of CrOx coating and N-H bonds density of CrWNx coating could explain about their lower polar component of surface energy. All the three PVD coatings remarkably reduced the quantity of tissue adhesion on the electrode from about 2 times (ZrOx and CrWNx coatings) to 4.88 times (CrOx coating) than uncoated SUS304 electrode.

Synthesis and Characterization of Sol-Gel Derived SiO2-CaO Glass

2015 ◽  
Vol 754-755 ◽  
pp. 964-968 ◽  
Author(s):  
S.A. Syed Nuzul Fadzli ◽  
S. Roslinda ◽  
Firuz Zainuddin
Keyword(s):  
Sol Gel ◽  
Xrd Analysis ◽  
Infrared Analysis ◽  
Carbonate Apatite ◽  
Binary Glass ◽  
Amorphous Glass ◽  
Sbf Solution ◽  
Free Glass

A phosphate-free glass based on SiO2-CaO was synthesized by a low temperature acid catalysed sol-gel route. The obtained material was evaluated by X-ray powder diffraction (XRD), Fourier transform infrared (FTIR), scanning electron microscope (SEM) and energy dispersive spectroscopy (EDS). The XRD analysis showed the sol-gel derived glass was amorphous in nature and crystallized when sintered at temperature above 900oC. The crystalline state was consisted of quartz and wollastonite phases. Meanwhile the infrared analysis showed typical transmission bands of monolith silica in the binary glass pattern. The bioactivity of the amorphous glass was investigated in vitro in simulated body fluid (SBF). The amorphous glasses were soaked in SBF for 1, 3 and 7 days. It is notable that the obtained results from FTIR, SEM and EDS analyses showed the deposition of spherical-shaped crystalline hydroxyl-carbonate-apatite (HCA) on the surface of the glass within 3 days of immersion in SBF solution. The HCA deposition was covered most of the surface after 7 days of immersion.

Effect of Carbonate Substitution on the Biomineralization of Apatite Bulk and Coating

2019 ◽  
Vol 807 ◽  
pp. 87-93
Author(s):  
Qing Xia Zhu ◽  
Quan Yi Nie
Keyword(s):  
Grain Size ◽  
Pore Size ◽  
Carbonate Apatite ◽  
Vitro Experiment ◽  
In Vitro Experiment ◽  
Carbonate Substitution ◽  
Experiment Method ◽  
Mineralization Processes ◽  
Sbf Solution

In-vitro experiment method was used to study biomineralization properties of carbonate apatite bulk and electrophoresis coating with different carbonate contents. The mineralization processes in SBF solution were investigated by means of XRD, SEM and so on. Research results show that the apatite with poor crystallization and small grain size induced by the carbonate substitution is beneficial to the sintering of the bulk and the formation of the bone-like hydroxyl-carbonate apatite (HCA) layer on the surface after soaking in the SBF. However, the carbonate substitution greatly reduces the pore size and porosity of coating, which is not conducive to the deposition and growth of the mineralized layer, thus weakens the biomineralization properties of the coating.

Microwave Synthesis of Nanocrystalline Hydroxyapatite and the Application in Bio-Composite

2018 ◽  
Vol 766 ◽  
pp. 228-232
Author(s):  
Pat Sooksaen ◽  
Kamonchanok Mekmork ◽  
Teeranuch Limprajuablarp ◽  
Sirichoke Kuharuangrong
Keyword(s):  
Composite Films ◽  
In Vitro Study ◽  
Poly Lactic Acid ◽  
Carbonate Apatite ◽  
Mixed Solution ◽  
Composite Surface ◽  
Nanocrystalline Hydroxyapatite ◽  
The Matrix ◽  
Sbf Solution

This research synthesized nanocrystalline hydroxyapatite (HA) via microwave assisted heating method. The morphology and crystallinity of nanocrystalline hydroxyapatite were affected by heating power and pH of the mixed solution. Nanocrystalline HA was best obtained when synthesized at pH = 11. Crystal size increased and crystal shape changed from spherical to rod and plate-like when the microwave power was increased from 320 to 800 watts. The synthesized nano-HA could be fabricated into bio-composite films using poly lactic acid (PLA) as the matrix and solvent casting method was utilized. In-vitro study for bioactivity was carried out in the SBF solution which formed hydroxyl-carbonate apatite (HCA) layer on the PLA/nano HA composite surface. The layer thickness increased in the background with increasing soaking time in the SBF.

scholarly journals Mechanical Properties and Bioactivity of Polyetheretherketone/Hydroxyapatite/Carbon Fiber Composite Prepared by the Mechanofusion Process

Polymers ◽  
2021 ◽  
Vol 13 (12) ◽  
pp. 1978
Author(s):  
In Sung Jeon ◽  
Moon Hyun Lee ◽  
Han-Hyeong Choi ◽  
Sangwoon Lee ◽  
Joon Woo Chon ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Carbon Fiber ◽  
Cell Attachment ◽  
Fiber Composite ◽  
Melt Processing ◽  
Carbon Fiber Composite ◽  
Ternary Composite ◽  
Cell Compatibility ◽  
Peek Composite

The main obstacles in the melt-processing of hydroxyapatite (HA) and carbon fiber (CF) reinforced polyetheretherketone (PEEK) composite are the high melting temperature of PEEK, poor dispersion of HA nanofillers, and poor processability due to high filler content. In this study, we prepared PEEK/HA/CF ternary composite using two different non-melt blending methods; suspension blending (SUS) in ethanol and mechanofusion process (MF) in dry condition. We compared the mechanical properties and bioactivity of the composite in a spinal cage application in the orthopedic field. Results showed that the PEEK/HA/CF composite made by the MF method exhibited higher flexural and compressive strengths than the composite prepared by the SUS method due to the enhanced dispersibility of HA nanofiller. On the basis of in vitro cell compatibility and cell attachment tests, PEEK/HA/CF composite by mechanofusion process showed an improvement in in vitro bioactivity and osteo-compatibility.

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