scholarly journals Development of a Highly Proliferated Bilayer Coating on 316L Stainless Steel Implants

Polymers ◽  
2020 ◽  
Vol 12 (5) ◽  
pp. 1022 ◽  
Author(s):  
Fatemeh Khosravi ◽  
Saied Nouri Khorasani ◽  
Shahla Khalili ◽  
Rasoul Esmaeely Neisiany ◽  
Erfan Rezvani Ghomi ◽  
...  
Keyword(s):  
Stainless Steel ◽  
Cell Attachment ◽  
Electrospun Nanofibers ◽  
Water Contact ◽  
Bilayer Coating ◽  
316 L Stainless Steel ◽  
Sbf Solution ◽  
Vitro Analysis ◽  
Cell Growth And Proliferation

In this research, a bilayer coating has been applied on the surface of 316 L stainless steel (316LSS) to provide highly proliferated metallic implants for bone regeneration. The first layer was prepared using electrophoretic deposition of graphene oxide (GO), while the top layer was coated utilizing electrospinning of poly (ε-caprolactone) (PCL)/gelatin (Ge)/forsterite solutions. The morphology, porosity, wettability, biodegradability, bioactivity, cell attachment and cell viability of the prepared coatings were evaluated. The Field Emission Scanning Electron Microscopy (FESEM) results revealed the formation of uniform, continuous, and bead-free nanofibers. The Energy Dispersive X-ray (EDS) results confirmed well-distributed forsterite nanoparticles in the structure of the top coating. The porosity of the electrospun nanofibers was found to be above 70%. The water contact angle measurements indicated an improvement in the wettability of the coating by increasing the amount of nanoparticles. Furthermore, the electrospun nanofibers containing 1 and 3 wt.% of forsterite nanoparticles showed significant bioactivity after soaking in the simulated body fluid (SBF) solution for 21 days. In addition, to investigate the in vitro analysis, the MG-63 cells were cultured on the PCL/Ge/forsterite and GO-PCL/Ge/forsterite coatings. The results confirmed an excellent cell adhesion along with considerable cell growth and proliferation. It should be also noted that the existence of the forsterite nanoparticles and the GO layer substantially enhanced the cell proliferation of the coatings.

In vitro study on the response of RAW264.7 and MS-5 fibroblast cells on laser-induced periodic surface structures for stainless steel alloys

RSC Advances ◽  
2015 ◽  
Vol 5 (53) ◽  
pp. 42548-42558 ◽  
Author(s):  
Clare McDaniel ◽  
Olga Gladkovskaya ◽  
Aiden Flanagan ◽  
Yury Rochev ◽  
Gerard M. O'Connor
Keyword(s):  
Stainless Steel ◽  
Surface Topography ◽  
Cell Attachment ◽  
In Vitro Study ◽  
Surface Structures ◽  
Fibroblast Cells ◽  
Steel Alloys ◽  
Periodic Surface ◽  
Stent Surface

Cell attachment and growth can be controlled by stent surface topography. In some cases fibroblast cells attach while monocytes failed on the structured surface of Pt:SS and 316LSS stents.

scholarly journals Enhanced in vitro biocompatibility and osteogenesis of titanium substrates immobilized with dopamine-assisted superparamagnetic Fe 3 O 4 nanoparticles for hBMSCs

2018 ◽  
Vol 5 (8) ◽  
pp. 172033 ◽  
Author(s):  
Zhenfei Huang ◽  
Zhihong Wu ◽  
Bupeng Ma ◽  
Lingjia Yu ◽  
Yu He ◽  
...  
Keyword(s):  
Cell Attachment ◽  
Clinical Applications ◽  
Bone Mesenchymal Stem Cells ◽  
Water Contact ◽  
Alizarin Red ◽  
In Vitro Biocompatibility ◽  
Related Transcription Factor ◽  
Nanoparticle Coatings ◽  
Bio Compatibility

Titanium (Ti) is an ideal bone substitute due to its superior bio-compatibility and remarkable corrosion resistance. However, in order to improve the osteoconduction and osteoinduction capacities in clinical applications, different kinds of surface modifications are typically applied to Ti alloys. In this study, we fabricated a tightly attached polydopamine-assisted Fe 3 O 4 nanoparticle coating on Ti with magnetic properties, aiming to improve the osteogenesis of the Ti substrates. The PDA-assisted Fe 3 O 4 nanoparticle coatings were characterized by scanning electron microscopy, energy dispersive spectroscopy, atomic force microscopy and water contact angle measurements. The cell attachment and proliferation rate of the human bone mesenchymal stem cells (hBMSCs) on the Ti surface significantly improved with the Fe 3 O 4 /PDA coating when compared with the pure Ti without a coating. Furthermore, the results of in vitro alkaline phosphatase (ALP) activity at 7 and 14 days and alizarin red S staining at 14 days showed that the Fe 3 O 4 /PDA coating on Ti promoted the osteogenic differentiation of hBMSCs. Moreover, hBMSCs co-cultured with the Fe 3 O 4 /PDA-coated Ti for approximately 14 days also exhibited a significantly higher mRNA expression level of ALP, osteocalcin and runt-related transcription factor-2 (RUNX2). Our in vitro results revealed that the present PDA-assisted Fe 3 O 4 nanoparticle surface coating is an innovative method for Ti surface modification and shows great potential for clinical applications.

scholarly journals Comparative short-term in vitro analysis of mutans streptococci adhesion on esthetic, nickel-titanium, and stainless-steel arch wires

2014 ◽  
Vol 84 (4) ◽  
pp. 680-686 ◽  
Author(s):  
In-Hye Kim ◽  
Hyo-Sang Park ◽  
Young Kyung Kim ◽  
Kyo-Han Kim ◽  
Tae-Yub Kwon
Keyword(s):  
Stainless Steel ◽  
Nickel Titanium ◽  
Mutans Streptococci ◽  
Short Term ◽  
Vitro Analysis ◽  
In Vitro Analysis

Fabrication and evaluation of polylactic acid/pectin composite scaffold via freeze extraction for tissue engineering

2020 ◽  
Vol 40 (5) ◽  
pp. 421-431
Author(s):  
Mohd Syahir Anwar Hamzah ◽  
Saiful Izwan Abd Razak ◽  
Mohammed Rafiq Abdul Kadir ◽  
Siti Pauliena Mohd Bohari ◽  
Nadirul Hasraf Mat Nayan ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Polylactic Acid ◽  
Cell Attachment ◽  
Porous Scaffold ◽  
Composite Scaffold ◽  
Engineering Material ◽  
Water Contact ◽  
Good Biocompatibility ◽  
Proliferation In Vitro

AbstractThis work reports the fabrication and characterizations of porous scaffold made up of polylactic acid (PLA) with the inclusion of pectin (1, 3, 5, 7, 9, 11 wt%) for potential tissue engineering material. The composite scaffold was prepared using a facile method of freeze extraction. Based on the physical evaluations, the scaffold was suggested to be optimum at 5 wt% of pectin loading. Water contact angle of the scaffold was significantly reduced to 46.5o with the inclusion of 5 wt% of pectin. Morphological and topographic of the PLA scaffold revealed that the pectin induced more porous structure and its surface became rougher which was suitable for cell attachment and proliferation. In vitro studies of the PLA/pectin composite scaffold using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromidelt (MTT) assay revealed good biocompatibility whereas Live-Dead kit assay resulted in 91% cell viability after 7 days of incubation.

Electrospun acetaminophen-loaded cellulose acetate nanofibers fabricated using an epoxy-coated spinneret

e-Polymers ◽  
2015 ◽  
Vol 15 (5) ◽  
pp. 311-315 ◽  
Author(s):  
Xia Wang ◽  
Xiao-Yan Li ◽  
Ying Li ◽  
Hua Zou ◽  
Deng Guang Yu ◽  
...  
Keyword(s):  
Stainless Steel ◽  
Cellulose Acetate ◽  
Amorphous State ◽  
Electrospun Nanofibers ◽  
Electrospinning Technique ◽  
Stainless Steel Capillary ◽  
Dissolution Tests ◽  
Model Drug ◽  
Functional Nanofibers

AbstractThis paper reports the investigation about the usage of an epoxy (EP)-coated spinneret for the preparation of medicated electrospun nanofibers. Cellulose acetate (CA) and acetaminophen (APAP) were used as the polymeric carrier and model drug, respectively. The electrospinning was undertaken using both EP-coated spinneret and traditional stainless steel capillary as spinnerets. According to the images from scanning electron microscopy, it is obvious that the nanofibers produced using the EP-coated spinneret had a finer diameter and a narrower size distribution (450±90 nm) than nanofibers fabricated using stainless steel equivalent (660±180 nm). In vitro dissolution tests revealed that the sustained-release profiles of nanofibers from the EP-coated spinneret were superior to those of their stainless steel equivalents, although APAP existed in a similar amorphous state in both nanofibers. Because the EP-coated material can exploit the electrical forces more effectively than its steel analogue, it can enhance the electrospinning technique for producing polymeric functional nanofibers.

scholarly journals Fabrication and Evaluation of Polycaprolactone/Gelatin-Based Electrospun Nanofibers with Antibacterial Properties

2015 ◽  
Vol 2015 ◽  
pp. 1-8 ◽  
Author(s):  
Lor Huai Chong ◽  
Mim Mim Lim ◽  
Naznin Sultana
Keyword(s):  
Drug Loading ◽  
Antibacterial Properties ◽  
Electrospun Nanofibers ◽  
Contact Angle Measurement ◽  
Angle Measurement ◽  
Electrospinning Technique ◽  
Water Contact ◽  
Nanofibrous Scaffolds ◽  
Model Drug

Nanofibrous scaffolds were fabricated through blending of a synthetic polymer, polycaprolactone (PCL), and a natural polymer, gelatin (GE), using an electrospinning technique. Processing and solution parameters were optimized to determine the suitable properties of PCL/GE-based nanofibers. Several characterizations were conducted to determine surface morphology by scanning electron microscopy (SEM), wettability using water contact angle measurement, and chemical bonding analysis using attenuated total reflectance (ATR) of PCL/GE-based nanofibers. Experimental results showed that 14% (w/v) PCL/GE with a flow rate of 0.5 mL/h and 18 kV demonstrated suitable properties. This nanofiber was then further investigated for itsin vitrodegradation, drug loading (using a model drug, tetracycline hydrochloride), and antibacterial testing (using zone inhibition method).

scholarly journals Targeting Delivery System for Lactobacillus Plantarum Based on Functionalized Electrospun Nanofibers

Polymers ◽  
2020 ◽  
Vol 12 (7) ◽  
pp. 1565
Author(s):  
Hongliang Yu ◽  
Weihua Liu ◽  
Dongmei Li ◽  
Chunhong Liu ◽  
Zhibiao Feng ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Laser Scanning ◽  
New Technologies ◽  
Electrospun Nanofibers ◽  
Electrospun Nanofiber ◽  
Bioactive Substances ◽  
Water Contact

With the increased interest in information on gut microbes, people are realizing the benefits of probiotics to health, and new technologies to improve the viability of probiotics are still explored. However, most probiotics have poor resistance to adverse environments. In order to improve the viability of lactic acid bacteria, polylactic acid (PLA) nanofibers were prepared by coaxial electrospinning. The electrospinning voltage was 16 kV, and the distance between spinneret and collector was 15 cm. The feed rates of the shell and core solutions were 1.0 and 0.25 mL/h, respectively. The lactic acid bacteria were encapsulated in the coaxial electrospun nanofibers with PLA and fructooligosaccharides (FOS) as the shell materials. Scanning electron microscopy, transmission electron microscopy, and laser scanning confocal microscopy showed that lactic acid bacteria were encapsulated in the coaxial electrospun nanofibers successfully. The water contact angle test indicated that coaxial electrospun nanofiber films had good hydrophobicity. An in vitro simulated digestion test exhibited that the survival rate of lactic acid bacteria encapsulated in coaxial electrospun nanofiber films was more than 72%. This study proved that the viability of probiotics can be improved through encapsulation within coaxial electrospun PLA nanofibers and provided a novel approach for encapsulating bioactive substances.

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