In Vitro Cytocompatibility of Silver-Containing Hydroxyapatite Coatings

2009 ◽  
Vol 620-622 ◽  
pp. 567-570 ◽  
Author(s):  
Yi Kai Chen ◽  
Xue Bin Zheng ◽  
Baoe Li ◽  
You Tao Xie ◽  
Chuan Xian Ding ◽  
...  
Keyword(s):  
Contact Angle ◽  
Antibacterial Property ◽  
Water Drop ◽  
Contact Angles ◽  
Hydroxyapatite Coating ◽  
Vacuum Plasma Spraying ◽  
Vacuum Plasma ◽  
Hydroxyapatite Coatings ◽  
Spraying Method

In this paper, antibacterial silver-containing hydroxyapatite coating was prepared by vacuum plasma spraying method and osteoblasts were seeded onto the surface of the coating to evaluate its cytocompatibility. The results indicated that the cells proliferated well on the samples, and the proliferation rate on the silver-containing hydroxyapatite coating was a little bit higher than that on the silver-free hydroxyapatite coating. The contact angle of water drop on the coating was measured, and it was found that the contact angles of the silver-containing hydroxyapatite coatings were smaller than the hydroxyapatite coating. The improvement of hydrophilicity for the silver-containing hydroxyapatite coating could be beneficial to the cells proliferation on its surface. It can be concluded that the addition of silver in the hydroxyapatite coating endowed the coating with antibacterial property while maintaining its excellent cytocompatibility.

Analysis of corrosion behaviour and surface properties of plasma-sprayed composite coating of hydroxyapatite–tantalum on biodegradable Mg alloy ZK60

2019 ◽  
Vol 53 (19) ◽  
pp. 2661-2673 ◽  
Author(s):  
Balraj Singh ◽  
Gurpreet Singh ◽  
Buta Singh Sidhu
Keyword(s):  
Corrosion Resistance ◽  
Surface Properties ◽  
Corrosion Behaviour ◽  
Surface Hardness ◽  
Mg Alloy ◽  
Hydroxyapatite Coating ◽  
Hydroxyapatite Coatings ◽  
Plasma Sprayed ◽  
Incremental Increase

Magnesium (Mg) and its alloys are promising candidates for biodegradable bio-implants. However, the excessive corrosion in the physiological environment and subsequent decline in the mechanical integrity of Mg and its alloys have limited their utility as biomaterials. In the present study, an attempt has been made to improve the corrosion resistance of Mg alloy ZK60 plasma sprayed with tantalum (Ta)-reinforced hydroxyapatite coating. The experiment was conducted with three varied levels, i.e. 10, 20 and 30 weight percent (wt%) of Ta-content in hydroxyapatite coating. The coatings were characterized and in vitro corrosion behaviour was investigated by electrochemical measurements in Ringer's solution along with the analysis of surface properties. The corrosion resistance of the Mg alloy increased with the incremental increase in Ta reinforcement in hydroxyapatite coating. An increase in the protection efficiency was analysed for the Ta-reinforced hydroxyapatite coatings (∼10%, 18% and 23% for hydroxyapatite-10Ta, hydroxyapatite-20Ta and hydroxyapatite-30Ta, respectively) as compared to the pure hydroxyapatite coating. The hydroxyapatite coating effectively increased the surface hardness of the Mg alloy and Ta reinforcement further enhanced it. Surface roughness decreased with the incremental increase in Ta-content in hydroxyapatite coating. Wettability analysis revealed the hydrophilic nature of pure hydroxyapatite and Ta-reinforced hydroxyapatite coatings. The results of the study suggest that the proposed Ta reinforcement in hydroxyapatite is potentially important for biodegradable Mg bio-implants.

In Vitro Assessment of Hydroxyapatite and Si-Substituted Hydroxyapatite Coatings under Different VPS Conditions

2008 ◽  
Vol 396-398 ◽  
pp. 345-348 ◽  
Author(s):  
Qian Tang ◽  
Roger Brooks ◽  
Serena Best
Keyword(s):  
Input Power ◽  
Precipitation Method ◽  
Human Osteoblast ◽  
Lower Plasma ◽  
Plasma Gun ◽  
Vacuum Plasma ◽  
Hydroxyapatite Coatings ◽  
Phase Pure ◽  
Plasma Sprayed

Hydroxyapatite and silicon-substituted hydroxyapatite powers were prepared in-house through a wet precipitation method and then vacuum plasma sprayed onto Ti-6Al-4V discs. Two plasma gun input powers were employed, 37 kW and 40 kW. All coatings were nearly phase pure, except small traces of impurities (TTCP, -TCP and CaO). Coatings prepared under the lower plasma gun input power had lower crystallinity. In vitro studies showed that human osteoblast-like cells attached and spread very well on all coated discs. Among the four kinds of discs, SiHAC37 was the most supportive to cell growth.

In Vitro Assessment of Antibacterial Activity and Cytotoxicity of Silver Contained Antibacterial HA Coating Material

2009 ◽  
Vol 620-622 ◽  
pp. 307-310 ◽  
Author(s):  
Zi Yuan Zhu ◽  
Fu Qiang Zhang ◽  
You Tao Xie ◽  
Yi Kai Chen ◽  
Xue Bin Zheng
Keyword(s):  
Antibacterial Activity ◽  
Zirconium Phosphate ◽  
Pathogenic Bacteria ◽  
Colorimetric Assay ◽  
Vacuum Plasma Spraying ◽  
Vacuum Plasma ◽  
Mtt Colorimetric Assay ◽  
Ha Coating ◽  
Covering Method

Failure of dental implant is often caused by specific pathogenic bacteria. In this paper, antibacterial HA coating was prepared by vacuum plasma spraying with silver loaded zirconium phosphate containing HA as feedstock. The antibacterial activity against Aa，Fn and Pg was evaluated using a film covering method. MTT colorimetric assay was used to measure cytotoxic effect of the antibacterial HA coatings. Results show that the HA coatings displayed significant antibacterial activity against Aa，Fn, Pg and no cytotoxicity to L929 murine cell lines when the content of silver-loaded zirconium phosphate antimicrobial is between 5wt% to 10wt%. The sensitivity of the bacteria to the antibacterial HA coating is in sequence of Pg >Fn >Aa.

Characterization of HA/Ta Composite Coatings Fabricated by Vacuum Plasma Spraying

2013 ◽  
Vol 761 ◽  
pp. 113-116 ◽  
Author(s):  
Li Ping Huang ◽  
You Tao Xie ◽  
Heng Ji ◽  
Yi Zeng ◽  
Xue Bin Zheng
Keyword(s):  
Plasma Spraying ◽  
Bonding Strength ◽  
Composite Coatings ◽  
X Ray Diffraction ◽  
Vacuum Plasma Spraying ◽  
Vacuum Plasma ◽  
Plasma Sprayed ◽  
Bonelike Apatite

Plasma sprayed hydroxyapatite (HA) coatings on titanium alloy substrates have been used extensively due to their excellent biocompatibility and osteoconductivity. However, the low bonding strength between HA and Ti substrates is still problematic in the long-term implantation lifespan. In this paper, HA/Ta composite coatings with various Ta contents (20% and 60%) were fabricated by vacuum plasma spraying (VPS). The microstructure, phase composition of the coatings was characterized by scanning electron microscopy (SEM) and X-ray diffraction (XRD), respectively. The results revealed that the coatings had a rough surface and lamellar structure including some pores. ASTM C-633 standard was used for the measurement of bonding strength, which was found increasing with the increase of Ta contents in the composite coatings. The bonding strength of the composite coating containing 60% Ta (H4T6) reached to 37.2 MPa, which is about 1.9 times that of HA coating. In vitro bioactivity evaluated in simulated body fluids (SBF) showed that bonelike apatite layer was formed on the composite coatings surface, which indicated the good bioactivity of the HA/Ta composite coatings.

scholarly journals Influence of Dentin Priming with Tannin-Rich Plant Extracts on the Longevity of Bonded Composite Restorations

2021 ◽  
Vol 2021 ◽  
pp. 1-10
Author(s):  
Mackeler Ramos Polassi ◽  
Thales de Sá Oliveira ◽  
Ana Calheiros de Carvalho ◽  
Lívia Soman de Medeiros Medeiros ◽  
Thiago André Moura Veiga ◽  
...  
Keyword(s):  
Contact Angle ◽  
Bond Strength ◽  
Plant Extracts ◽  
In Vitro Study ◽  
Adhesive System ◽  
Contact Angles ◽  
Control Group ◽  
Dentin Surface ◽  
Rhus Chinensis

Objective. This in vitro study evaluated the influence of bioactive plant extracts as dentin biomodifying agents to improve the longevity of bonded restorations. For that, plant extracts were applied to the dentin surface prior to the adhesive system. Materials and Methods. Bovine incisors were ground flat to obtain 2 mm thick slices in which conical preparations were made (N = 10). Tannin-containing plant extracts were applied to dentin before the application of the restorative system, as follows: control group (untreated, CTL), chlorhexidine 0.12% (CHX), mastruz (Dysphania ambrosioides, MTZ), cat’s claw (Uncaria tomentosa, CTC), guarana (Paullinia cupana, GUA), galla chinensis (Rhus chinensis, GCH), and tannic acid (extracted from Acacia decurrens, TNA). The push-out bond strength test was conducted (0.5 mm/min). Dentin biomodification was assessed by the modulus of elasticity and mass change in bovine tooth sections (0.5 × 1.7 × 7.0 mm). The dentin staining after extract treatments of dentin slices was compared. The dentin surface wettability was also evaluated by means of the contact angles of the adhesive system with the dentin surface and compared with the untreated control group. Data were subjected to ANOVA/Tukey’s test (α = 0.05). Results. The bond strength of the restoratives to dentin was not significantly improved by the plant extracts, irrespective of the evaluation time ( p > 0.05 ). Except for TNA, the elastic modulus of demineralized dentin significantly reduced after treatment with the plant extracts ( p < 0.05 ). The dentin staining correlated with the tannin content of the extracts. The contact angle was significantly reduced when treated with CTC, GCH, and TNA. Conclusions. The tannin-containing extracts had a questionable effect on the longevity of bonded restorations. The dentin modulus was negatively affected by the extract treatments. Although some of the extracts changed the contact angle, which seems to improve the adhesive monomer permeation, the tannin-rich plant extract application prior to adhesive application was proven to be clinically unfeasible due to dentin staining.

Construction of a bioactive surface with micro/nano-topography on titanium alloy by micro-milling and alkali-hydrothermal treatment

2016 ◽  
Vol 230 (12) ◽  
pp. 1086-1095 ◽  
Author(s):  
Teng Wang ◽  
Yi Wan ◽  
Zhaojun Kou ◽  
Yukui Cai ◽  
Bing Wang ◽  
...  
Keyword(s):  
Contact Angle ◽  
Titanium Alloy ◽  
Surface Topography ◽  
Laser Scanning ◽  
Hydrothermal Reaction ◽  
Contact Angles ◽  
Micro Milling ◽  
In Vitro Tests ◽  
Structured Surface

The surface topography and wettability are important factors that determine the biocompatibility of biomaterials. In this article, the hierarchical micro/nano-topography of titanium alloy surface was fabricated by micro-milling and alkali-hydrothermal reaction. The surface topography and chemical composition of treated surfaces were characterized using laser scanning microscope and scanning electron microscope. The contact angles of surfaces with different micro/nano-topographies were measured by contact angle tester. MC3T3s morphology and osteocalcin productions were characterized to investigate the influence of surface modification on implants’ biocompatibility. The results show that hydrophilicity of micro-structured surface decreased compared to the untextured surface and contact angle values decreased with the increase in micro-groove spacing in small increments. In addition, the surfaces treated with alkali-hydrothermal reaction displayed strong hydrophilicity and the surface energy increased by 40 nJ/cm2 approximately. In vitro tests indicated that micro/nano-structured surface improved the adhesion, spreading, and differentiation of MC3T3s.

scholarly journals Impact of catheter contact angle on lesion formation and durability of pulmonary vein isolation

2021 ◽  
Author(s):  
Masayuki Ohta ◽  
Kentaro Hayashi ◽  
Hiroyuki Sato ◽  
Takahiro Noto ◽  
Kandoh Kawahatsu ◽  
...  
Keyword(s):  
Contact Angle ◽  
Observational Study ◽  
Pulmonary Vein ◽  
Pulmonary Vein Isolation ◽  
Contact Angles ◽  
Retrospective Observational Study ◽  
Lesion Formation ◽  
Vitro Experiment ◽  
In Vitro Experiment

Introduction Little is known on the effect of catheter contact angle on lesion formation and durability of pulmonary vein isolation (PVI). Methods Both in vitro experiment and retrospective observational study were conducted. For in vitro experiment, radiofrequency lesions were created on explanted swine hearts in three different catheter contact angles (0°, 45°, and 90°). In the retrospective observational study, we assessed patients who had undergone repeat catheter ablation due to atrial fibrillation recurrence after initial PVI. When pulmonary vein (PV) reconnection was observed, we analyzed the previous ablation points within and without the gap area. The gap areas were defined where ablation had changed the PV activation sequence or eliminated the PV potential in the repeat session. Results In the in vitro experiment, lesion width was the smallest (5.3 ± 0.4 mm) in perpendicular contact compared to 0° (vs 5.8 ± 0.5 mm, p=0.040) and 45° (vs 6.4 ± 0.4 mm, p<0.001). In the retrospective observational study, we assessed 666 tags of 16 patients with PV reconnections, and 60 tags were in the gap area. Tags in the gap area had longer interlesion distance (odds ratio [OR] 1.49, p < 0.001), greater contact force variability (OR 1.03, p = 0.008), and higher rate of perpendicular contact (OR 3.26, p < 0.001) on multivariate analysis. Conclusion Perpendicular contact was associated with a smaller lesion and higher rate of PV reconnection.

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