scholarly journals Extrusion Printed Graphene/Polycaprolactone/Composites for Tissue Engineering

2013 ◽  
Vol 773-774 ◽  
pp. 496-502 ◽  
Author(s):  
Sepidar Sayyar ◽  
Rhys Cornock ◽  
Eoin Murray ◽  
Stephen Beirne ◽  
David L. Officer ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Tissue Engineering ◽  
Three Dimensional ◽  
Promising Material ◽  
Additive Fabrication ◽  
Printing System ◽  
Good Biocompatibility ◽  
Covalently Linked ◽  
Extrusion Printing

In this work fibres and complex three-dimensional scaffolds of a covalently linked graphene-polycaprolactone composite were successfully extruded and printed using a melt extrusion printing system. Fibres with varying diameters and morphologies, as well as complex scaffolds were fabricated using an additive fabrication approach and were characterized. It was found that the addition of graphene improves the mechanical properties of the fibres by over 50% and in vitro cytotoxicity tests showed good biocompatibility indicating a promising material for tissue engineering applications.

scholarly journals Microstructure design and degradation performance in vitro of three-dimensional printed bioscaffold for bone tissue engineering

2019 ◽  
Vol 11 (10) ◽  
pp. 168781401988378 ◽  
Author(s):  
Hongyu Jin ◽  
Yue Zhuo ◽  
Yang Sun ◽  
Hongya Fu ◽  
Zhenyu Han
Keyword(s):  
Mechanical Properties ◽  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Polylactic Acid ◽  
Tricalcium Phosphate ◽  
Three Dimensional ◽  
Spherical Pore ◽  
And Topology

In bone tissue engineering, three-dimensional printed biological scaffolds play an important role in the development of bone regeneration. The ideal scaffolds should have the ability to match the bone degradation rate and osteogenic ability. This article optimizes the unit cell model of the microstructure including spherical pore, gyroid, and topology to explore degradation performance of scaffolds. Boolean operation of array microstructure unit cells and selected part of a computer-aided design (CAD) femur model are adopted to create a reconstructed scaffold model. Polylactic acid/[Formula: see text]-tricalcium phosphate/hydroxyapatite scaffolds with spherical pore, gyroid, and topology-optimized structures are manufactured by three-dimensional printing utilizing the composition of bio-ink including polylactic acid, [Formula: see text]-tricalcium phosphate, and hydroxyapatite. After degradation of the scaffolds in vitro for several days, the mechanical properties are analyzed to study the effects of different microstructures on the degradation properties. The results show that the gyroid scaffolds with favorable degradability still maintain excellent mechanical properties after degradation. Mechanical properties of the scaffolds with topology-optimized structure and spherical pore microstructure scaffolds have a significant decrease after degradation.

scholarly journals Mineralization of Biomaterials for Bone Tissue Engineering

2020 ◽  
Vol 7 (4) ◽  
pp. 132
Author(s):  
Xinchen Wu ◽  
Kierra Walsh ◽  
Brianna L. Hoff ◽  
Gulden Camci-Unal
Keyword(s):  
Mechanical Properties ◽  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Regeneration ◽  
Bone Tissue ◽  
Three Dimensional ◽  
Surface Characteristics ◽  
Cell Recruitment ◽  
Carbonated Apatite

Mineralized biomaterials have been demonstrated to enhance bone regeneration compared to their non-mineralized analogs. As non-mineralized scaffolds do not perform as well as mineralized scaffolds in terms of their mechanical and surface properties, osteoconductivity and osteoinductivity, mineralization strategies are promising methods in the development of functional biomimetic bone scaffolds. In particular, the mineralization of three-dimensional (3D) scaffolds has become a promising approach for guided bone regeneration. In this paper, we review the major approaches used for mineralizing tissue engineering constructs. The resulting scaffolds provide minerals chemically similar to the inorganic component of natural bone, carbonated apatite, Ca5(PO4,CO3)3(OH). In addition, we discuss the characterization techniques that are used to characterize the mineralized scaffolds, such as the degree of mineralization, surface characteristics, mechanical properties of the scaffolds, and the chemical composition of the deposited minerals. In vitro cell culture studies show that the mineralized scaffolds are highly osteoinductive. We also summarize, based on literature examples, the applications of 3D mineralized constructs, as well as the rationale behind their use. The mineralized scaffolds have improved bone regeneration in animal models due to the enhanced mechanical properties and cell recruitment capability making them a preferable option for bone tissue engineering over non-mineralized scaffolds.

Poly(glycerol sebacate)—A Novel Biodegradable Elastomer for Tissue Engineering

2002 ◽  
Vol 724 ◽  
Author(s):  
Yadong Wang ◽  
Barbara J. Sheppard ◽  
Robert Langer
Keyword(s):  
Tissue Engineering ◽  
Three Dimensional ◽  
Hydroxyl Groups ◽  
Random Coils ◽  
Dimensional Network ◽  
Implantation Sites ◽  
Good Biocompatibility ◽  
Normal Architecture

AbstractBiodegradable polymers have significant potential in biotechnology and bioengineering. However, for some applications, they are limited by their inferior mechanical properties and unsatisfactory compatibility with cells and tissues. A strong, biodegradable, and biocompatible elastomer could be useful for fields such as tissue engineering, drug delivery, and in vivo sensing [1, 2]. We designed, synthesized, and characterized a tough biodegradable elastomer from biocompatible monomers. This elastomer forms a covalently crosslinked three-dimensional network of random coils with hydroxyl groups attached to its backbone. Both crosslinking and the hydrogen bonding interactions between the hydroxyl groups likely contributes to the unique properties of the elastomer. In vitro and in vivo studies show the polymer has good biocompatibility. Subcutaneous (SC) polymer implants are absorbed completely within 60 days with restoration of the implantation sites to their normal architecture.

scholarly journals Is Dialdehyde Chitosan a Good Substance to Modify Physicochemical Properties of Biopolymeric Materials?

2021 ◽  
Vol 22 (7) ◽  
pp. 3391
Author(s):  
Sylwia Grabska-Zielińska ◽  
Alina Sionkowska ◽  
Ewa Olewnik-Kruszkowska ◽  
Katarzyna Reczyńska ◽  
Elżbieta Pamuła
Keyword(s):  
Mechanical Properties ◽  
Tissue Engineering ◽  
Physicochemical Properties ◽  
Silk Fibroin ◽  
Three Dimensional ◽  
Microscopy Imaging ◽  
Maximum Deformation ◽  
One Step ◽  
Chitosan Blends ◽  
Fourier Transfer Infrared Spectroscopy

The aim of this work was to compare physicochemical properties of three dimensional scaffolds based on silk fibroin, collagen and chitosan blends, cross-linked with dialdehyde starch (DAS) and dialdehyde chitosan (DAC). DAS was commercially available, while DAC was obtained by one-step synthesis. Structure and physicochemical properties of the materials were characterized using Fourier transfer infrared spectroscopy with attenuated total reflectance device (FTIR-ATR), swelling behavior and water content measurements, porosity and density observations, scanning electron microscopy imaging (SEM), mechanical properties evaluation and thermogravimetric analysis. Metabolic activity with AlamarBlue assay and live/dead fluorescence staining were performed to evaluate the cytocompatibility of the obtained materials with MG-63 osteoblast-like cells. The results showed that the properties of the scaffolds based on silk fibroin, collagen and chitosan can be modified by chemical cross-linking with DAS and DAC. It was found that DAS and DAC have different influence on the properties of biopolymeric scaffolds. Materials cross-linked with DAS were characterized by higher swelling ability (~4000% for DAS cross-linked materials; ~2500% for DAC cross-linked materials), they had lower density (Coll/CTS/30SF scaffold cross-linked with DAS: 21.8 ± 2.4 g/cm3; cross-linked with DAC: 14.6 ± 0.7 g/cm3) and lower mechanical properties (maximum deformation for DAC cross-linked scaffolds was about 69%; for DAS cross-linked scaffolds it was in the range of 12.67 ± 1.51% and 19.83 ± 1.30%) in comparison to materials cross-linked with DAC. Additionally, scaffolds cross-linked with DAS exhibited higher biocompatibility than those cross-linked with DAC. However, the obtained results showed that both types of scaffolds can provide the support required in regenerative medicine and tissue engineering. The scaffolds presented in the present work can be potentially used in bone tissue engineering to facilitate healing of small bone defects.

scholarly journals Development of Biopolymeric Hybrid Scaffold-Based on AAc/GO/nHAp/TiO2 Nanocomposite for Bone Tissue Engineering: In-Vitro Analysis

Nanomaterials ◽  
2021 ◽  
Vol 11 (5) ◽  
pp. 1319
Author(s):  
Muhammad Umar Aslam Khan ◽  
Wafa Shamsan Al-Arjan ◽  
Mona Saad Binkadem ◽  
Hassan Mehboob ◽  
Adnan Haider ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Guar Gum ◽  
Porous Scaffolds ◽  
Vitro Assay ◽  
Vitro Analysis ◽  
Nanocomposite Scaffolds

Bone tissue engineering is an advanced field for treatment of fractured bones to restore/regulate biological functions. Biopolymeric/bioceramic-based hybrid nanocomposite scaffolds are potential biomaterials for bone tissue because of biodegradable and biocompatible characteristics. We report synthesis of nanocomposite based on acrylic acid (AAc)/guar gum (GG), nano-hydroxyapatite (HAp NPs), titanium nanoparticles (TiO2 NPs), and optimum graphene oxide (GO) amount via free radical polymerization method. Porous scaffolds were fabricated through freeze-drying technique and coated with silver sulphadiazine. Different techniques were used to investigate functional group, crystal structural properties, morphology/elemental properties, porosity, and mechanical properties of fabricated scaffolds. Results show that increasing amount of TiO2 in combination with optimized GO has improved physicochemical and microstructural properties, mechanical properties (compressive strength (2.96 to 13.31 MPa) and Young’s modulus (39.56 to 300.81 MPa)), and porous properties (pore size (256.11 to 107.42 μm) and porosity (79.97 to 44.32%)). After 150 min, silver sulfadiazine release was found to be ~94.1%. In vitro assay of scaffolds also exhibited promising results against mouse pre-osteoblast (MC3T3-E1) cell lines. Hence, these fabricated scaffolds would be potential biomaterials for bone tissue engineering in biomedical engineering.

scholarly journals Three-dimensionally printed polycaprolactone/multicomponent bioactive glass scaffolds for potential application in bone tissue engineering

2020 ◽  
Vol 6 (1) ◽  
pp. 57-69
Author(s):  
Amirhosein Fathi ◽  
Farzad Kermani ◽  
Aliasghar Behnamghader ◽  
Sara Banijamali ◽  
Masoud Mozafari ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
3D Printing ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Three Dimensional ◽  
Layer By Layer ◽  
Composite Scaffolds ◽  
3D Printed ◽  
Co Doped

AbstractOver the last years, three-dimensional (3D) printing has been successfully applied to produce suitable substitutes for treating bone defects. In this work, 3D printed composite scaffolds of polycaprolactone (PCL) and strontium (Sr)- and cobalt (Co)-doped multi-component melt-derived bioactive glasses (BGs) were prepared for bone tissue engineering strategies. For this purpose, 30% of as-prepared BG particles (size <38 μm) were incorporated into PCL, and then the obtained composite mix was introduced into a 3D printing machine to fabricate layer-by-layer porous structures with the size of 12 × 12 × 2 mm3.The scaffolds were fully characterized through a series of physico-chemical and biological assays. Adding the BGs to PCL led to an improvement in the compressive strength of the fabricated scaffolds and increased their hydrophilicity. Furthermore, the PCL/BG scaffolds showed apatite-forming ability (i.e., bioactivity behavior) after being immersed in simulated body fluid (SBF). The in vitro cellular examinations revealed the cytocompatibility of the scaffolds and confirmed them as suitable substrates for the adhesion and proliferation of MG-63 osteosarcoma cells. In conclusion, 3D printed composite scaffolds made of PCL and Sr- and Co-doped BGs might be potentially-beneficial bone replacements, and the achieved results motivate further research on these materials.

Three-Dimensional High Resolution Scaffold Fiber Architecture and Morphology in Tissue Engineered Heart Valve Tissue

2010 ◽  
Author(s):  
Chad E. Eckert ◽  
Brandon T. Mikulis ◽  
Dane Gerneke ◽  
Danielle Gottlieb ◽  
Bruce Smaill ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
High Resolution ◽  
Heart Valve ◽  
Structural Information ◽  
Three Dimensional ◽  
Tissue Scaffold ◽  
Valve Tissue ◽  
Sampling Protocols ◽  
Heart Valve Tissue

Engineered heart valve tissue (EHVT) has received much attention as a potential pediatric valve replacement therapy, offering prospective long-term functional improvements over current options. A significant gap in the literature exists, however, regarding estimating tissue mechanical properties from tissue-scaffold composites. Detailed three-dimensional structural information prior to implantation (in vitro) and after implantation in (in vivo) is needed for improved modeling of tissue properties. As such, a novel high-resolution imaging technique will be employed to obtain three-dimensional microstructural information. Analysis techniques will be used to fully quantify constituents of interest including scaffold, collagen, and cellular information and to develop appropriate two-dimensional sectioning sampling protocols. It is the intent of this work to guide modeling efforts to better elucidate EHVT tissue-specific mechanical properties.

Silicate-doped nano-hydroxyapatite/graphene oxide composite reinforced fibrous scaffolds for bone tissue engineering

2018 ◽  
Vol 32 (10) ◽  
pp. 1392-1405 ◽  
Author(s):  
Ali Deniz Dalgic ◽  
Ammar Z. Alshemary ◽  
Ayşen Tezcaner ◽  
Dilek Keskin ◽  
Zafer Evis
Keyword(s):  
Tissue Engineering ◽  
Graphene Oxide ◽  
Bone Tissue Engineering ◽  
Protein Adsorption ◽  
Bone Tissue ◽  
Three Dimensional ◽  
Osteosarcoma Cell ◽  
In Vitro Biocompatibility ◽  
Microscopy Techniques

In this study, novel graphene oxide–incorporated silicate-doped nano-hydroxyapatite composites were prepared and their potential use for bone tissue engineering was investigated by developing an electrospun poly(ε-caprolactone) scaffold. Nanocomposite groups were synthesized to have two different ratios of graphene oxide (2 and 4 wt%) to evaluate the effect of graphene oxide incorporation and groups with different silicate-doped nano-hydroxyapatite content was prepared to investigate optimum concentrations of both silicate-doped nano-hydroxyapatite and graphene oxide. Three-dimensional poly(ε-caprolactone) scaffolds were prepared by wet electrospinning and reinforced with silicate-doped nano-hydroxyapatite/graphene oxide nanocomposite groups to improve bone regeneration potency. Microstructural and chemical characteristics of the scaffolds were investigated by X-ray diffraction, Fourier transform infrared spectroscope and scanning electron microscopy techniques. Protein adsorption and desorption on material surfaces were studied using fetal bovine serum. Presence of graphene oxide in the scaffold, dramatically increased the protein adsorption with decreased desorption. In vitro biocompatibility studies were conducted using human osteosarcoma cell line (Saos-2). Electrospun scaffold group that was prepared with effective concentrations of silicate-doped nano-hydroxyapatite and graphene oxide particles (poly(ε-caprolactone) – 10% silicate-doped nano-hydroxyapatite – 4% graphene oxide) showed improved adhesion, spreading, proliferation and alkaline phosphatase activity compared to other scaffold groups.

Investigation on Biocompatibility and Mechanical Properties of Ti15Mg Alloy

2021 ◽  
Vol 1039 ◽  
pp. 557-564
Author(s):  
Haydar Abdul Hassan Al-Ethari ◽  
Sundus Abbas Jasim ◽  
Ekhlas Khalid Zamel
Keyword(s):  
Mechanical Properties ◽  
Wear Behavior ◽  
Research Work ◽  
Wear Test ◽  
Optical Microscope ◽  
Alloy Sample ◽  
Microscope Analysis ◽  
Good Biocompatibility ◽  
Prepared Alloy

In this research work, bioactive Ti15Mg alloy was prepared by powder metallurgy route to investigate its biocompatibility and mechanical properties. Many tests were performed including X-ray diffraction; optical microscope analysis, scanning electron microscope analysis, ultrasonic wave test, corrosion behavior test, Static immersion test, and the wet sliding wear test. The XRD result shows that the prepared alloy sample consist of (α-Ti phase) and Mg. The microstructure of the prepared alloy sample consisted of a biodegradable Mg or pore and alpha titanium. The effect of the Mg content on degradability was tested based on simulated body fluid of Ringer solutions using electrochemical corrosion. The findings indicate that an elastic modulus of 47GPa exhibits the alloy. There were low corrosion rates of the alloy. The Ti matrix remained integrity after 14 days of immersion in the Ringer solutions, and the magnesium phase dissolved in the solution, causing a layer to form on the alloy. The wear behavior of the prepared ally at wet sliding conditions was evaluated using pin on disc method. The in vitro analysis showed good biocompatibility with Ti15Mg alloy. The prepared alloy demonstrates good biocompatibility and bioactivity.

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