scholarly journals Role of total motile sperm count in the evaluation of young men with bilateral subclinical varicocele and asthenospermia

2020 ◽  
Vol 92 (4) ◽  
Author(s):  
Georgios Tsampoukas ◽  
Athanasios Dellis ◽  
Antigoni Katsouri ◽  
Dominic Brown ◽  
Konstantinos Deliveliotis ◽  
...  
Keyword(s):  
At Risk ◽  
Class Group ◽  
Semen Quality ◽  
Sperm Count ◽  
Motile Sperm ◽  
Class A ◽  
Significant Difference ◽  
Group 2 ◽  
Total Motile Sperm Count ◽  
Group 1

Introduction: In comparison to its clinical analogue, the subclinical varicocele represents a questionable entity and specific guidelines for the optimal management are lacking. In our previous study of patients with subclinical varicocele, we showed that bilateral condition is associated with risk of dyspermia. In the present study, we evaluated the risk of deterioration of semen quality in men with bilateral disease and impaired motility according to WHO criteria. Materials and methods: Men with bilateral subclinical varicocele, not desiring fatherhood at the time of presentation, were included in study. During initial evaluation, the number of Total Motile Sperm Count (TMSC) was calculated and the patients’ age, total testicular volume (TTV), maximum venous size and mean resistive index (RI) of the intratesticular arteries were recorded. We classified the participants in five classes according to the TMSC reading: class A-: TMSC < 5 x 106, class A: TMSC between 5-10 x 106, class B: TMSC between 10-15 x 106, class C: TMSC between 15-20 x 106, and class D: TMSC > 20 x 106 per ejaculate. The participants were seen after 6 months for a repeat spermiogram and physical examination. If clinical varicocele was diagnosed or a new abnormality in the spermiogram was noted, the participants were excluded from the study. The remaining patients were allocated to two groups according to the repeat TMSC reading: patients sub-classified into a lower class (group 1), and patients remaining at the same class (group 2). A comparative analysis was performed between two groups. Results: Nineteen men were included. Nine patients were subclassified (group 1). Three patients moved to A- class (< 5 x 106). Ten patients remained in the same class having no deterioration (group 2). Comparing the two groups, no statistically significant difference was recognized for age, TTV, maximum venous size on both sides, and mean RI (p > 0.05). However, the initial reading for TMSC was 14.57 x 106 in group 1, and 22.84 x 106 in group 2, respectively. This difference was statistically significant (p < 0.05). Additionally, in a paired analysis there was a significant difference in TMSC after 6 months (p < 0.05), too. Summary Conclusions: Young men with bilateral varicocele and asthenospermia seem to be at risk of deterioration in their semen quality after a follow-up of 6 months. The measurement of TMSC can unmask patients at risk, whereas men with the lowest readings seem to be at highest risk for deterioration. The possibility of a worsening sperm quality should be considered in the appropriate clinical context.

scholarly journals Impact of percutaneous embolization versus subinguinal microsurgical ligation on semen parameters in primary varicocele patients: comparative study

2020 ◽  
Vol 51 (1) ◽  
Author(s):  
Haytham M. Nasser ◽  
Ahmed Hussein ◽  
Gad M. Behairy ◽  
Mostafa Abdo
Keyword(s):  
Sperm Count ◽  
Semen Analysis ◽  
Statistical Significance ◽  
Semen Parameters ◽  
Male Factor ◽  
Percutaneous Embolization ◽  
Significant Difference ◽  
Group 2 ◽  
Group 1

Abstract Background Varicocele is an abnormally dilated pampiniform plexus of the veins within the spermatic cord and is considered the most common correctable cause of male factor infertility. Many approaches are described for treatment either surgical (tradition inguinal, subinguinal, and laparoscopic) or non-surgical percutaneous embolization. During the period from August 2017 to December 2018, we prospectively analyzed the preoperative and post-operative alteration of semen parameters (at 3 and 9 months) of the data collected from 63 patients with clinically evident varicocele referred to our tertiary hospital. Patients were divided into two groups: group 1, thirty-three patients who underwent subinguinal microsurgical ligation, and group 2, thirty patients who underwent percutaneous embolization. Results Sixty-three patients enrolled in this study were divided in two groups: group 1, patients who underwent surgery, and group 2, patients who underwent embolization; the mean age is 24.6 ± 1.27 years in group 1 and 23.7 ± 2 years in group 2; there was no statistically significant difference between the two groups as regards BMI, diabetes, hypertension, and smoking. Bilaterality was present in 15.2% of group 1 patients and 10% in group 2 patients (P value 0.06). Most of the patients were classified as grades 2 and 3 with no statistical significance regarding severity of the disease. Preoperative semen parameters for patients including sperm count, motility, and abnormal forms showed no statistically significant difference between the two groups. Post-intervention semen analysis was done twice during follow-up after 3 months and 9 months from the date of intervention. After 3 months, the semen parameters were improved in both groups in spite of the higher sperm count in group 2 but with no statistical significance. After 9 months follow-up, semen analysis showed persistent increase in sperm mobility in group 1 patients in comparison to group 2 patients. Both groups had better improvement in count of normal form with no statistical significant change. Conclusion Improvement of semen parameters while treating primary varicocele by either subinguinal microsurgery approach or percutaneous embolization shows equivalent outcomes.

scholarly journals MP26-14 EXTERNAL VALIDATION OF THE POST-VARICOCELECTOMY SEMEN QUALITY NOMOGRAM TO PREDICT TOTAL MOTILE SPERM COUNT

2020 ◽  
Vol 203 ◽  
pp. e405-e406
Author(s):  
Hyung Ho Lee ◽  
Won Sik Jang ◽  
Ji Eun Heo ◽  
Jong Soo Lee ◽  
Ki Hong Kim ◽  
...  
Keyword(s):  
Semen Quality ◽  
Sperm Count ◽  
External Validation ◽  
Motile Sperm ◽  
Total Motile Sperm Count

scholarly journals Investigation of the effect of body mass index (BMI) on semen parameters and male reproductive system hormones

2017 ◽  
Vol 89 (3) ◽  
pp. 219 ◽  
Author(s):  
Mehmet Zeynel Keskin ◽  
Salih Budak ◽  
Evrim Emre Aksoy ◽  
Cem Yücel ◽  
Serkan Karamazak ◽  
...  
Keyword(s):  
Body Mass Index ◽  
Body Mass ◽  
Sperm Count ◽  
Reproductive Hormones ◽  
Semen Parameters ◽  
Total Testosterone ◽  
Significant Difference ◽  
Group 3 ◽  
Group 2 ◽  
Group 1

Aim: To evaluate the effects of body mass index (BMI) ratio on semen parameters and serum reproductive hormones. Materials and methods: The data of 454 patients who prsented to male infertility clinics in our hospital between 2014 and 2015 were analyzed retrospectively. Weight, height, serum hormone levels and semen analysis results of the patients were obtained. BMI values were calculated by using the weight and height values of the patients and they were classified as group 1 for BMI values ≤ 25 kg/m2, as group 2 for BMI values 25-30 kg/m2 and as group 3 for BMI values ≥ 30 kg/m2. Results: The mean values of BMI, semen volume, concentration, total motility, progressive motility, total progressive motile sperm count (TPMSC), normal morphology according to Kruger, head abnormality, neck abnormality, tail abnormality, FSH, LH, prolactin, T/E2, total testosterone and estradiol parameters of the patients were considered. Patients were divided according to BMI values in Group 1 (n = 165), Group 2 (n = 222) and Group 3 (n = 56). There was no statistically significant difference in terms of all variables between the groups. Conclusions: We analyzed the relationship between BMI level and semen parameters and reproductive hormones, demonstrating no relationship between BMI and semen parameters. In our study, BMI does not affect semen parameters although it shows negative correlation with prolactin and testosterone levels.

scholarly journals The Effect of Metabolic Syndrome upon the Success of Varicocelectomy

2012 ◽  
Vol 2012 ◽  
pp. 1-3 ◽  
Author(s):  
Ufuk Ozturk ◽  
Nevzat Can Sener ◽  
Ismail Nalbant ◽  
Osman Raif Karabacak ◽  
Mustafa Gurhan Ulusoy ◽  
...  
Keyword(s):  
Metabolic Syndrome ◽  
Sperm Count ◽  
Sperm Parameters ◽  
Spermatic Vein ◽  
Motile Sperm ◽  
Spontaneous Pregnancy ◽  
Normal Sperm ◽  
Group 2 ◽  
The Impact ◽  
Group 1

We aimed to investigate the impact of metabolic syndrome (MetS) on the varicocele treatment. 101 patients underwent spermatic vein ligation between 2007 and 2010 were retrospectively analyzed. Those patients were divided into two groups as without (n: 56, Group 1) or with MetS (n: 48, Group 2). All the patients underwent left microsurgical subinguinal spermatic vein ligation. Groups were compared by the improvement on sperm parameters and spontaneous pregnancy rates at a mean of 19 (±4) months followup. When sperm parameters were compared postoperatively, the significant improvement in total sperm count, motile sperm count percentage, and normal sperm percentage was reported. The groups were compared to each other and the improvement seemed significantly better in Group 1. There was no statistically significant improvement difference in the normal sperm percentage between groups. Spontaneous pregnancy rate after two years was 45% in Group 1 and 34% in Group 2 (). Patients with MetS and varicocele improved after surgery, but not as well as the similar group without MetS. This may help to show that MetS can be a factor for male infertility.

scholarly journals Laparoscopic varicocelectomy in male infertility

2021 ◽  
Author(s):  
Felix Seiler ◽  
Philipp Kneissl ◽  
Claudius Hamann ◽  
Klaus-Peter Jünemann ◽  
Daniar Osmonov
Keyword(s):  
Sperm Count ◽  
University Hospital ◽  
Semen Parameters ◽  
Nonobstructive Azoospermia ◽  
Sperm Density ◽  
Reproductive Techniques ◽  
Group 2 ◽  
Total Motile Sperm Count ◽  
Group 1

Summary Background The suitability of laparoscopic varicocelectomy for assisted reproductive technology depends on the improvement of semen parameters. The present study analyzed the improvement of semen parameters following laparoscopic varicocele ligation. Material and methods A retrospective study of the laparoscopic varicocele clippings at the Department of Urology of University Hospital of Kiel between the years 2007 and 2019 was conducted. The semen analyses according to WHO standards (sperm count, density, motility and morphology) were conducted before and 12 months after surgery. Screening for surgical complications took place at the time of the follow-up seminal analysis. Included were patients with oligozoospermia, asthenozoospermia and/or teratozoospermia (group 1, OAT) or with nonobstructive azoospermia (group 2, NOA). Results This study included data of 27 patients and 22 patients presented preoperative OAT (81%, group 1). Another 5 patients showed NOA (19%, group 2). Data of group 1 showed that semen parameters normalized in 32% of the patients after surgery. Significant improvement in total sperm count (p < 0.005), sperm density (p < 0.005) and total motile sperm count (p < 0.005) was observed. No deterioration of semen parameters was observed. In group 2 we detected spermatozoa in 1 case in the postoperative ejaculate. None of the patients showed complications according to the Clavien-Dindo classification, postoperative hydrocele formation or recurrence of varicocele at the time of control spermiogram. Conclusion Laparoscopic varicocelectomy is a valid therapeutic approach to improve semen parameters for further assisted reproductive techniques. Spermatogenesis may be induced for patients with NOA. Normalization of semen parameters can be achieved for patients with OAT.

scholarly journals Mitochondria Content and Activity Are Crucial Parameters for Bull Sperm Quality Evaluation

Antioxidants ◽  
2021 ◽  
Vol 10 (8) ◽  
pp. 1204
Author(s):  
Zofia E. Madeja ◽  
Marta Podralska ◽  
Agnieszka Nadel ◽  
Marcin Pszczola ◽  
Piotr Pawlak ◽  
...  
Keyword(s):  
Quality Evaluation ◽  
Semen Quality ◽  
Sperm Quality ◽  
Transcript Level ◽  
Glutathione Synthetase ◽  
Motile Sperm ◽  
Bovine Sperm ◽  
Cryopreserved Sperm ◽  
Group 2 ◽  
Group 1

Standard sperm evaluation parameters do not enable predicting their ability to survive cryopreservation. Mitochondria are highly prone to suffer injuries during freezing, and any abnormalities in their morphology or function are reflected by a decline of sperm quality. Our work focused on describing a link between the number and the activity of mitochondria, with an aim to validate its applicability as a biomarker of bovine sperm quality. Cryopreserved sperm collected from bulls with high (group 1) and low (group 2) semen quality was separated by swim up. The spermatozoa of group 1 overall retained more mitochondria (MitoTrackerGreen) and mtDNA copies, irrespective of the fraction. Regardless of the initial ejaculate quality, the motile sperm contained significantly more mitochondria and mtDNA copies. The same trend was observed for mitochondrial membrane potential (ΔΨm, JC-1), where motile sperm displayed high ΔΨm. These results stay in agreement with transcript-level evaluation (real-time polymerase chain reaction, PCR) of antioxidant enzymes (PRDX1, SOD1, GSS), which protect cells from the reactive oxygen species. An overall higher level of glutathione synthetase (GSS) mRNA was noted in group 1 bulls, suggesting higher ability to counteract free radicals. No differences were noted between basal oxygen consumption rate (OCR) (Seahorse XF Agilent) and ATP-linked respiration for group 1 and 2 bulls. In conclusion, mitochondrial content and activity may be used as reliable markers for bovine sperm quality evaluation.

scholarly journals Association between BMI and semen quality: an observational study of 3966 sperm donors

2018 ◽  
Vol 34 (1) ◽  
pp. 155-162 ◽  
Author(s):  
Jixuan Ma ◽  
Li Wu ◽  
Yun Zhou ◽  
Hai Zhang ◽  
Chengliang Xiong ◽  
...  
Keyword(s):  
Observational Study ◽  
Semen Quality ◽  
Sperm Count ◽  
Sperm Concentration ◽  
Normal Weight ◽  
Motile Sperm ◽  
Sperm Number ◽  
Sperm Donors ◽  
Semen Volume ◽  
Total Motile Sperm Count

Abstract STUDY QUESTION What is the relationship between abnormal BMI and semen quality? SUMMARY ANSWER Underweight was significantly associated with lower sperm concentration, total sperm number and total motile sperm count, while overweight was significantly associated with lower semen volume, total sperm number and total motile sperm count. WHAT IS KNOWN ALREADY Abnormal BMI has been associated with lower semen quality, but the results remain somewhat controversial. In addition, most previous studies have focused on the influence of obesity or overweight on semen quality, and evidence on the association between underweight and semen quality is rare. STUDY DESIGN, SIZE, DURATION This research was an observational study investigating 3966 sperm donors from a large sperm bank in Wuhan city, China. These donors passed the screening for sperm donation and underwent 29 949 semen examinations between 1 January 2013 and 9 April 2018. PARTICIPANTS/MATERIALS, SETTING, METHODS BMI was categorized into four groups: underweight (<18.5 kg/m2), normal weight (18.5–24.9 kg/m2), overweight (25–29.9 kg/m2) and obese (≥30 kg/m2). Semen volume, sperm concentration, total sperm number, total motility, progressive motility and total motile sperm count were determined by trained clinical technicians. Linear mixed models were used to conduct dose–response analyses between BMI and semen quality parameters. MAIN RESULTS AND THE ROLE OF CHANCE Underweight was significantly associated with a 3.0% (95% CI: 0.1%, 5.8%), 6.7% (1.9%, 11.3%) and 7.4% (2.2%, 12.4%) reduction in sperm concentration, total sperm number and total motile sperm count, respectively. Overweight was significantly associated with a 4.2% (1.6%, 6.8%), 3.9% (0.9%, 6.9%) and 3.6% (0.2%, 6.9%) reduction in semen volume, total sperm number and total motile sperm count, respectively. Non-linear models including continuous BMI as a natural cubic spline function yielded similar results. LIMITATIONS, REASONS FOR CAUTION Our study subjects were sperm donors who are typically young and healthy, and therefore not representative of the general male population. Caution should be paid in generalizing our results to other populations. Furthermore, we did not measure the donors’ weight repeatedly along with each semen donation; instead, we only measured it once during the screening, which may cause bias due to the variations of weight across time. WIDER IMPLICATIONS OF THE FINDINGS Our study provides evidence that underweight and overweight are associated with lower semen quality, and highlights the importance of maintaining a normal weight for men. STUDY FUNDING/COMPETING INTEREST(S) This work was supported by the Health and Family Planning Commission of Hubei Province (Grant number WJ2015MA027), the Hubei Provincial Committee of the Communist Youth League of China, and Center for Global and Regional Environmental Research at the University of Iowa. The authors declare that there are no conflicts of interest. TRIAL REGISTRATION NUMBER N/A.

Decline in semen quality among infertile men during the past 15 years in Western Algeria (SIDI-BEL-ABBES)

2016 ◽  
Vol 6 (4) ◽  
pp. 112-118
Author(s):  
El-haina Fatima Zohra ◽  
Bendahmane Malika
Keyword(s):  
Semen Quality ◽  
Sperm Count ◽  
Linear Regression Analysis ◽  
Semen Parameters ◽  
Motile Sperm ◽  
Infertile Men ◽  
Semen Volume ◽  
Western Algeria ◽  
Study Population ◽  
Total Motile Sperm Count

The current study aimed at measuring changes in semen quality and quantity in young healthy sperm of the partner in western Algeria over 15 years. A retrospective analysis of semen parameters over 15 years was carried out using linear regression analysis, at the fertility laboratory in West Algeria. Study population consisted of 188 young partners, aged 25 to 35 years, who donated sperm samples for artificial insemination between 2000 and 2015. Their wives with infertility problems were surveyed using questionnaire about their lifestyles and infertility factors. There were no statistically significant changes in semen concentration and motility during the study period. The mean semen volume increased by 0.1 mL per year (P < 0.0001), with a concomitant mean rise of 5.8 × 106 per year in total motile sperm count. The percentage of sperm normal morphology decreased by a mean of 1% per year during the entire period (P < 0.0001). During these 15 past years, there has been an increase in total motile sperm count, and semen volume where-as, a decline in normal sperm morphology has been noticed that is independ-ent of the age and the duration of abstinence of infertile men.

scholarly journals Land use and semen quality: A fertility center cohort study

PLoS ONE ◽  
2021 ◽  
Vol 16 (8) ◽  
pp. e0255985
Author(s):  
Seung-Ah Choe ◽  
Seulgi Kim ◽  
Changmin Im ◽  
Sun-Young Kim ◽  
Gregory Wellenius ◽  
...  
Keyword(s):  
Built Environment ◽  
Fresh Water ◽  
Semen Quality ◽  
Sperm Count ◽  
Positive Association ◽  
Semen Parameters ◽  
Exposure Model ◽  
Motile Sperm ◽  
Progressive Motility ◽  
Total Motile Sperm Count

This study explored the association between built environment and semen parameters among men who sought fertility evaluation. We used a data of 5,886 men living in the Seoul capital area whose semen was tested at a single fertility center during 2016–2018. Distance to fresh water, the coast, major roadways, and neighborhood greenness measured by Normalized Difference Vegetation Index (NDVI) were evaluated. Outcome indicators were semen volume, sperm concentration, percentage of progressive motility, vitality, normal morphology, and total motile sperm count. Linear regression models were fitted to standardized values of six semen indicators. Majority of men were white-collar, clerical, and service workers. Linear associations between built environment features and semen quality indicators were not evident except for NDVI within 500 m and sperm vitality (β = 0.05; 95% confidence interval (CI): 0.01, 0.09). The 2nd quartile of distance to fresh water was associated with lower progressive motility compared to the 1st quartile (β = −0.10; 95% CI: −0.17, −0.03). Proportion of vitality was higher among men in the 2nd quartile of distance to roadways than those in the 1st quartile (0.08; 95% CI: 0.01, 0.15). Men in the 2nd quartile of NDVI had higher total motile sperm count (0.09; 95% CI: 0.01, 0.17). In the multi-exposure model, the positive association between NDVI and vitality remained (0.03; 95% CI: 0.00, 0.06). We observed potential evidence regarding the impact of built environment on male fertility, specifically a positive association between residential greenness and sperm vitality among men with a history of infertility.

In-vivo evaluation of the effect of cyanoacrylate on prosthetic vascular graft infection – does cyanoacrylate increase the severity of infection?

VASA ◽  
2020 ◽  
Vol 49 (4) ◽  
pp. 281-284
Author(s):  
Atıf Yolgosteren ◽  
Gencehan Kumtepe ◽  
Melda Payaslioglu ◽  
Cuneyt Ozakin
Keyword(s):  
Vascular Graft ◽  
Graft Infection ◽  
Vascular Graft Infection ◽  
Group 4 ◽  
Significant Difference ◽  
Group 3 ◽  
Group 2 ◽  
Prosthetic Vascular Graft Infection ◽  
Group 1

Summary. Background: Prosthetic vascular graft infection (PVGI) is a complication with high mortality. Cyanoacrylate (CA) is an adhesive which has been used in a number of surgical procedures. In this in-vivo study, we aimed to evaluate the relationship between PVGI and CA. Materials and methods: Thirty-two rats were equally divided into four groups. Pouch was formed on back of rats until deep fascia. In group 1, vascular graft with polyethyleneterephthalate (PET) was placed into pouch. In group 2, MRSA strain with a density of 1 ml 0.5 MacFarland was injected into pouch. In group 3, 1 cm 2 vascular graft with PET piece was placed into pouch and MRSA strain with a density of 1 ml 0.5 MacFarland was injected. In group 4, 1 cm 2 vascular graft with PET piece impregnated with N-butyl cyanoacrylate-based adhesive was placed and MRSA strain with a density of 1 ml 0.5 MacFarland was injected. All rats were scarified in 96th hour, culture samples were taken where intervention was performed and were evaluated microbiologically. Bacteria reproducing in each group were numerically evaluated based on colony-forming unit (CFU/ml) and compared by taking their average. Results: MRSA reproduction of 0 CFU/ml in group 1, of 1410 CFU/ml in group 2, of 180 200 CFU/ml in group 3 and of 625 300 CFU/ml in group 4 was present. A statistically significant difference was present between group 1 and group 4 (p < 0.01), between group 2 and group 4 (p < 0.01), between group 3 and group 4 (p < 0.05). In terms of reproduction, no statistically significant difference was found in group 1, group 2, group 3 in themselves. Conclusions: We observed that the rate of infection increased in the cyanoacyrylate group where cyanoacrylate was used. We think that surgeon should be more careful in using CA in vascular surgery.

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