scholarly journals IMPACT OF MULTIPLEX PCR IN REDUCING THE RISK OF RESIDUAL TRANSFUSION-TRANSMITTED HUMAN IMMUNODEFICIENCY AND HEPATITIS B AND C VIRUSES IN BURKINA FASO

2018 ◽  
Vol 10 (1) ◽  
pp. e2018041 ◽  
Author(s):  
Arzouma Paul YOODA ◽  
Serge Theophile SOUBEIGA ◽  
Kompingnin Yacouba NEBIE ◽  
Birama DIARRA ◽  
Salam SAWADOGO ◽  
...  
Keyword(s):  
Blood Transfusion ◽  
Hepatitis B ◽  
Burkina Faso ◽  
Multiplex Pcr ◽  
Blood Donation ◽  
Cross Sectional Study ◽  
Residual Risk ◽  
Serological Tests ◽  
Cross Sectional ◽  
The Impact

Background and ObjectiveThe improved performance of serological tests has significantly reduced the risk of human immunodeficiency and hepatitis B and C viruses transmission by blood transfusion, but there is a persistence of residual risk. The objective of this study was to evaluate the impact of multiplex PCR in reducing the risk of residual transmission of these viruses in seronegative blood donors in Burkina Faso.MethodsThis cross-sectional study was conducted from March to September 2017. The serological tests were performed on sera using ARCHITECTSR i1000 (Abbot diagnosis, USA). Detection of viral nucleic acids was performed by multiplex PCR on mini-pools of seronegative plasma for HBV, HCV and HIV using SaCycler-96 Real Time PCR v.7.3 (Sacace Biotechnologies). Multiplex PCR-positive samples from these mini-pools were then individually tested by the same method.Results A total of 989 donors aged 17 to 65 were included in the present study. "Repeat donors" accounted for 44.79% (443/989). Seroprevalences for HIV, HBV, and HCV were 2.53% (25/989), 7.28% (72/989) and 2.73% (27/989), respectively. Of the 14 co-infections detected, HBV/HCV was the most common with 0.71% (7/989) of cases. Of 808 donations tested by multiplex PCR, 4.70% (38/808) were positive for HBV while no donation was positive for HIV or HCV.Conclusion: Our study showed a high residual risk of HBV transmission through blood transfusion. Due to the high prevalence of blood-borne infections in Burkina Faso, we recommend the addition of multiplex PCR to serologic tests for optimal blood donation screening.

scholarly journals Knowledge, attitudes and perceptions among non blood donor female health care professionals

2015 ◽  
Vol 8 (4) ◽  
pp. 203 ◽  
Author(s):  
Muhammad Bilal ◽  
Abdul Haseeb ◽  
Ibrahim Zahid ◽  
Sehan Siraj Lashkerwala ◽  
Fawad Saeeduddin ◽  
...  
Keyword(s):  
Blood Transfusion ◽  
Hepatitis B ◽  
Health Care Professionals ◽  
Blood Donors ◽  
Blood Donation ◽  
Tertiary Care ◽  
Cross Sectional Study ◽  
Sample Population ◽  
Cross Sectional ◽  
Adequate Knowledge

<p>Introduction<strong>: </strong>Blood donation is necessary in order to maintain an adequate supply of blood to patients who are suffering from any kind of disease or trauma, which requires them to have blood transfusion. Female non-blood donors are generally low in number.<strong> </strong>Therefore, this research was carried out to assess the main reasons behind the lack of blood donations made by females, and their knowledge, attitude and perceptions towards voluntary blood donation.</p> <p>Methodology: A cross-sectional study was conducted on 664 female health professionals, who were selected by non-probability convenience sampling from two tertiary care hospitals. A pretested questionnaire was presented to the sample population, and the data was entered and analyzed on SPSS (V17).</p> <p>Results: 94.6 % were aware with the fact that blood is screened for AIDS, Hepatitis B and C before transfusion. Moreover, 83.7% said that they will only donate blood if a family, relative or friend would need it and similarly 83.4%  suggested that they would donate blood if blood donation camps are arranged in hospital premises. 81.8 % thought that blood donors can contract Hepatitis B after donation where as only 29.5% did not blood due already blood loss in menstrual cycle.<strong></strong></p> <p>Conclusion<strong>: </strong>The participants had adequate knowledge about the benefits of blood donation. The most important reason identified for not donating blood is the lack of facilities within the workplace or lack of approach by responsible authorities. The results of the study may help in minimizing the misconceptions of the participants about blood transfusion, which would increase their contribution towards blood donation.</p>

scholarly journals HEPATITIS B AND HEPATITIS C;

2018 ◽  
Vol 25 (08) ◽  
pp. 1245-1251
Author(s):  
Mustansar Billah ◽  
Syed Muhammad Raza Shah ◽  
Muhammad Mujtaba Hashir
Keyword(s):  
Hepatitis B ◽  
Blood Donors ◽  
Blood Donation ◽  
Tertiary Hospital ◽  
Cross Sectional Study ◽  
Blood Bank ◽  
Research Centre ◽  
Cross Sectional ◽  
Medicine Department ◽  
And Gender

Objectives: To determine the frequency of HBV and HCV among blood donorscoming voluntarily at the blood bank of Ibne- Siena hospital and research centre, Multan,Pakistan, to ascertain the disease burden in the community for their future prevention andcontrol. Study Design: It was a descriptive, cross- sectional study on blood units donated byhealthy individuals coming voluntarily for blood donation, and willing to get their blood testedfor HBV, HCV. Setting: Community medicine department of Multan medical and dental collegeMultan, and blood bank of affiliated tertiary hospital, Ibn-e- Siena hospital Multan. Period: Sixmonths period from Jan 2016 to June 2016. Materials and Methods: Data of the blood donorswas collected. Present study was based on previous well maintained records of hospital bloodbank. During the reported period, a total of 1195 blood donors attended the blood bank, andthey all were screened for HBsAg and anti-HCV. SPSS- 16 was used to analyze the data, fordetermination of frequencies of Hepatitis- B, and C according to the age and gender. Results:Total numbers of blood donors were 1195. 1191 were males, and 4 were females. All thefemales were negative for both HBV, and HCV. Males positive for HBV were 18 (1.5 %), andthose Positive for HCV were 27 (3.2%).

A Cross-Sectional Study of Sociodemographic Profile of Blood Donors and Their Knowledge about Blood Donation in a Rural Area of Kerala

2021 ◽  
Vol 8 (29) ◽  
pp. 2615-2619
Author(s):  
Indu Pachampully Kumaran ◽  
Archana K.A ◽  
Meena Dharmadas ◽  
Sasikala Nadanganan ◽  
Anu Kumar B ◽  
...  
Keyword(s):  
Blood Transfusion ◽  
Blood Donor ◽  
Blood Donors ◽  
Blood Donation ◽  
Cross Sectional Study ◽  
Director General ◽  
Sectional Study ◽  
Donor Motivation ◽  
Cross Sectional ◽  
Communication Modalities

BACKGROUND Even in the presence of sufficient voluntary blood donors, the need of blood is not met yet. We wanted to evaluate the causes that hinder the smooth functioning of well-maintained blood transfusion services (BTS). METHODS This is a population based cross-sectional study conducted among blood donors from voluntary outreach blood donation camp for period of one year. Total 1536 blood donors from 32 blood donation camps were subjected to study for the sociodemographic characteristic and their knowledge of blood donation. Statistical analysis of data was done using Microsoft Excel, analysed using statistical package for social sciences (SPSS) ver.16 software. RESULTS A total of 32 blood donation camps were conducted during the study period that provided 1536 donors. In most of the camps, male donors (75 %) were more than female donors. Donors of age group of 30 to 39 years (43.9 %) were more when compared to others. Donors those who were married accounted for 79.9 %. Blood donors of Hindu religion (53 %) were more. CONCLUSIONS The main hindrance for blood donor motivation is lack of knowledge. Literacy and chance to mingle in society have a positive effect on blood donor motivation. Targeting of effective donor motivation, recruitment, and retention were attained by conducting awareness classes, seminars, workshops, and visual communication modalities. KEYWORDS World Health Organization, Altruistic Blood Donor, Blood Transfusion Services, Transfusion Transmitted Infections, Blood Components and Plasma Derivatives, Director General of Health Services (DGHS), National Blood Transportation Council (NBTC)

scholarly journals Hepatitis B and C Virus Infections among Blood Donors in Blood Transfusion Center, Pokhara, Nepal: Seroprevalence and its Associated Risk Factors

2019 ◽  
Vol 6 (1) ◽  
pp. 33-38
Author(s):  
Gyanendra Bikram Shah ◽  
Krishna Gurung ◽  
Bharat Prasad Baral ◽  
Ram Raja Gurung ◽  
Balkrishna Bhattachan
Keyword(s):  
Risk Factors ◽  
Blood Transfusion ◽  
Hepatitis B ◽  
Infection Rate ◽  
Blood Donors ◽  
Cross Sectional Study ◽  
Hcv Infection ◽  
Volunteer Blood Donor ◽  
Cross Sectional ◽  
Infected People

Hepatitis B Virus (HBV) and Hepatitis C Virus (HCV) infections lead to chronic diseases and are the most common causes of liver cirrhosis and cancer in developing countries like Nepal. The study is carried out to determine the seroprevalence of HBV and HCV by using a Rapid kit method and Elisa Method to find out its risk factors. The cross-sectional study was done among blood donating people from 16th August 2016 to 19th November 2016. Blood donors in Pokhara Valley were screened for anti-HCV antibodies, anti-HBV antibodies using third generation ELISA kits and automated ELISA Processor in serology laboratory at Central Blood Transfusion Service (CBTS) of Nepal Red Cross Society (NRCS) in Pokhara, Nepal. 1777 (87.2%) units were male blood donors and 260 (12.6%) units were female donors out of 2037 participants. Gender wise, the ratio between male and female was 1:0.1. HBV and HCV infection rate in blood donors were detected at 0.7% (15/2037) and 0.5% (8/2037) respectively. HBV infection rate in volunteer blood donor people was 0.7% (14/1881) which was higher than the replacement donors i.e. 0.6% (1/156). Similarly, in HCV infection rate in volunteer donor were 0.4% (8/1881). HBV infected people are detected higher than the HCV infected people among the blood donors. In addition, there was no significant relationship between positive results of HBV and HCV tests with the gender, age, tattoo, donor type.

scholarly journals Distribution and Incidence of Blood-Borne Infection among Blood Donors from Regional Transfusion Centers in Burkina Faso: A Comprehensive Study

2021 ◽  
Vol 104 (4) ◽  
pp. 1577-1581
Author(s):  
Nicha Wongjarupong ◽  
Sharad Oli ◽  
Mahamoudou Sanou ◽  
Florencia Djigma ◽  
Alice Kiba Koumare ◽  
...  
Keyword(s):  
Hepatitis B ◽  
Burkina Faso ◽  
Urban Area ◽  
Rural Area ◽  
Blood Donors ◽  
Blood Donation ◽  
Hepatitis B Surface Antigen ◽  
Residual Risk ◽  
Syphilis Infection ◽  
Comprehensive Study

ABSTRACTThere is a high prevalence of blood-borne infections in West Africa. This study sought to determine the seroprevalence of blood-borne infections, including hepatitis B virus (HBV), hepatitis C virus (HCV), HIV, and syphilis, in blood donors in Burkina Faso. Blood donors were recruited from 2009 to 2013 in four major cities in Burkina Faso of urban area (Ouagadougou) and rural area (Bobo Dioulasso, Fada N’Gourma, and Ouahigouya). Serology tests including hepatitis B surface antigen, anti-HCV, anti-HIV, and rapid plasma reagin test were used for screening and were confirmed with ELISA. Disease prevalence was calculated among first-time donors. Incidence and residual risk were calculated from repeat donors. There were 166,681 donors; 43,084 had ≥ 2 donations. The overall seroprevalence of HBV, HCV, HIV, and syphilis were 13.4%, 6.9%, 2.1%, and 2.4%, respectively. The incidence rates (IRs) of HBV, HCV, HIV, and syphilis infection were 2,433, 3,056, 1,121, and 1,287 per 100,000 person-years. There was lower seroprevalence of HBV and HCV in urban area than in rural area (12.9% versus 14.0%, P < 0.001; and 5.9% versus 8.0%, P < 0.001), and no difference in HIV (2.1% versus 2.1%, P = 0.25). The IRs of new HBV, HCV, HIV, and syphilis were 2.43, 3.06, 1.12, and 1.29 per 100,000 person-years, respectively. The residual risk was one per 268 donations for HBV, one per 181 donations for HCV, and one per 1,480 donations for HIV, respectively. In conclusion, this comprehensive study from four blood donation sites in Burkina Faso showed high HBV and HCV seroprevalence and incidence with high residual risk from blood donation.

scholarly journals The prevalence of human immunodeficiency and of hepatitis B viral infections is not increased in patients with sickle cell disease in Tanzania

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Grace Shayo ◽  
Irene Makundi ◽  
Lucio Luzzatto
Keyword(s):  
Sickle Cell Disease ◽  
Blood Transfusion ◽  
Hepatitis B ◽  
Sickle Cell ◽  
Viral Infections ◽  
Cross Sectional Study ◽  
Dar Es Salaam ◽  
Categorical Variables ◽  
Cell Disease ◽  
Cross Sectional

Abstract Background Tanzania ranks as the fourth country in the world with respect to the number of sickle cell disease (SCD) births; it is also endemic to the human immunodeficiency virus (HIV) and the hepatitis B virus (HBV). This study was done to determine the prevalence of HIV and HBV infections among SCD patients in Dar es Salaam, Tanzania. Methods A multicenter hospital-based descriptive cross sectional study was carried out among participants aged ≥ 16 years with a proven diagnosis of SCD. Socio-demographic and clinical data were recorded. Blood samples were drawn for HIV and HBV diagnosis. All categorical variables were summarized into frequencies. Results There were 185/325 (56.9 %) females. The mean age (SD) was 23.0 ± 7.5 years. The prevalence of HIV was 1.8 %; the prevalence of HBV was 1.2 %. Conclusions The prevalence of both HIV and HBV in SCD patients is no greater than in the general population of Dar es Salaam or Tanzania. For associations, a large study would be needed. From a detailed blood transfusion history of SCD patients we found no evidence that HIV or HBV infection was transmitted through blood transfusion.

scholarly journals Prevalence and risk factors for HIV, syphilis, hepatitis B, hepatitis C, and HTLV-I/II infection in low-income postpartum and pregnant women in Greater Metropolitan Vitória, Espírito Santo State, Brazil

2009 ◽  
Vol 25 (3) ◽  
pp. 668-676 ◽  
Author(s):  
Lucia Helena Mello de Lima ◽  
Maria Carmen Viana
Keyword(s):  
Risk Factors ◽  
Hepatitis B ◽  
Pregnant Women ◽  
Low Income ◽  
Cross Sectional Study ◽  
Serological Tests ◽  
Cross Sectional ◽  
Espírito Santo ◽  
Factors Associated ◽  
Espirito Santo

Sexually transmitted infections during pregnancy pose a major risk to the fetus due to vertical transmission. The study's objective was to determine the prevalence of HIV, syphilis, hepatitis B and C, and HTLV-I/II infection among low-income postpartum and pregnant women treated in Greater Metropolitan Vitória, Espírito Santo State, Brazil, and the risk factors associated with these infections. A cross-sectional study was conducted from February to October 1999 assessing postpartum and pregnant women from the maternity ward of the Vitória Mercy Hospital and the Carapina Outpatient Referral Unit in the Municipality of Serra, respectively. Patients were systematically interviewed and had blood samples drawn for serological tests (HIV 1&2, VDRL, HbsAg, anti-HCV, and HTLV-I/II). A total of 534 patients (332 postpartum and 202 pregnant women) were assessed. Seroprevalence rates for the target infections in postpartum and pregnant women and the overall sample were as follows, respectively: HIV 0.9%, 0%, and 0.6%; syphilis 2.1%, 3.6%, and 2.7%; HBV 1.2%, 1%, and 1.1%; HCV 1.8%, 0.6%, and 1.4%; and HTLV-I/II 1.7%, 0.6%, and 1.3%. Factors associated with the various infections are presented and analyzed in light of other research findings from the literature.

scholarly journals Epidemiological, serological and molecular aspects of hepatitis B and C in children and teenagers of municipal daycare facilities schools and schools in the city of Santos

2014 ◽  
Vol 17 (3) ◽  
pp. 588-599 ◽  
Author(s):  
Maria Célia Cunha Ciaccia ◽  
Regina Célia Moreira ◽  
Marcílio Figueiredo Lemos ◽  
Isabel Takano Oba ◽  
Gilda Porta
Keyword(s):  
Elementary Education ◽  
Hepatitis B ◽  
Cross Sectional Study ◽  
Serological Markers ◽  
Serological Tests ◽  
Cross Sectional ◽  
Acceptable Error ◽  
Molecular Aspects ◽  
Education Network ◽  
The City

Objective: To estimate the prevalence of the serological markers anti-HBc, HBsAg and anti-HBs of hepatitis B and anti-HCV of hepatitis C among children and teenagers enrolled at daycare facilities, kindergartens and municipal elementary education network in the city of Santos, São Paulo, Brazil. Methods: A cross-sectional study was carried out from June 28 to December 14, 2007, in which 4,680 finger-prick blood samples were collected from children and teenagers. A survey questionnaire was applied to their family members. The sample was dimensioned using the software Epi Info version 6 with expected frequency of 1%, acceptable error of 0.5% and confidence interval of 95%. The serological tests were performed using the ELISA technique. The molecular analysis was performed using the technique of polymerase chain reaction in House. Results: Age of the studied population ranged from 7 months to 18 years and 1 month. The general prevalence of anti-HBc reagent was 0.1%, HBsAg was 0.02% and anti-HCV was 0.02%. Conclusions: In children, the general prevalence of serological markers for hepatitis B and C in the city of Santos was low when compared with literature data.

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