scholarly journals Evaluations of bacterial contaminated full thickness burn wound healing in Sprague Dawley rats Treated with Tualang honey

2011 ◽  
Vol 44 (1) ◽  
pp. 112 ◽  
Author(s):  
AhmadSukari Halim ◽  
Kirnpal KaurBanga Singh ◽  
SalmiMohamed Sukur
Keyword(s):  
Wound Healing ◽  
Full Thickness ◽  
Sprague Dawley ◽  
Burn Wound ◽  
Burn Wound Healing ◽  
Sprague Dawley Rats ◽  
Tualang Honey

scholarly journals Evaluations of bacterial contaminated full thickness burn wound healing in Sprague Dawley rats Treated with Tualang honey

2011 ◽  
Vol 44 (01) ◽  
pp. 112-117 ◽  
Author(s):  
Salmi Mohamed Sukur ◽  
Ahmad Sukari Halim ◽  
Kirnpal Kaur Banga Singh
Keyword(s):  
Wound Healing ◽  
Full Thickness ◽  
Sprague Dawley ◽  
Burn Wounds ◽  
Wound Size ◽  
Sprague Dawley Rats ◽  
Study Results ◽  
The Mean ◽  
Tualang Honey ◽  
Chitosan Gel

ABSTRACT Aim: The effect of Tualang honey on wound healing in bacterial contaminated full-thickness burn wounds was evaluated in 36 male Sprague Dawley rats. Materials and Methods: The rats were randomly divided into three groups (n = 12/group). Three full-thickness burn wounds were created on each rat. Each group of rats was inoculated with a different organism in the burn wounds: Group A was inoculated with Pseudomonas aeruginosa, Group B was inoculated with Klebsiella pneumoniae and Group C was inoculated with Acinetobacter baumannii. One wound on each rat was dressed with either Tualang honey, Chitosan gel or Hydrofibre silver. Each wound size was measured on day 3, 6, 9, 12, 15, 18 and 21 of the study. Results: The mean wound size of the Tualang honey-treated wounds was not statistically different than that of the Chitosan gel or Hydrofibre silver-treated wounds when the wounds were compared throughout the entire experiment (P > 0.05). However, comparing the mean wound size on day 21 alone revealed that the Tualang honey-treated wounds were smaller in comparison to that of the Chitosan gel and Hydrofibre silver-treated groups. Conclusions: This study shows that topical application of Tualang honey on burn wounds contaminated with P. aeruginosa and A. baumannii gave the fastest rate of healing compared with other treatments.

scholarly journals PENGARUH INFUSA DAUN KEMBANG BULAN (Tithonia difersifolia) PADA KECEPATAN PENYEMBUHAN LUKA BAKAR

2018 ◽  
Vol 12 (3) ◽  
pp. 230
Author(s):  
Dwi Mulyani
Keyword(s):  
Wound Healing ◽  
Positive Control ◽  
Negative Control ◽  
Full Thickness ◽  
Sprague Dawley ◽  
Burn Wound ◽  
Rat Models ◽  
Sprague Dawley Rats ◽  
Healing Wound ◽  
Heated Metal

<p><em>The aim of this investigation was a clinical</em><em> </em><em>evaluation of the</em><em> </em><em>accelerating effect of </em><em>Tithonia difersifolia </em><em>extract<strong> </strong></em><strong><em> </em></strong><em>on the burn </em><a title="Find more articles at http://www.scialert.net/asci/result.php?searchin=Keywords&amp;cat=&amp;ascicat=ALL&amp;Submit=Search&amp;keyword=wound+healing (wound healing)" href="http://www.scialert.net/asci/result.php?searchin=Keywords&amp;cat=&amp;ascicat=ALL&amp;Submit=Search&amp;keyword=wound+healing" target="_blank"><em>wound healing</em></a><em> in male Sprague-Dawley rats weighted 20</em><em>0</em><em>-</em><em>3</em><em>5</em><em>0</em><em>.</em><em> T</em><em>he  sample then extracted using wet rendering (infusa) method using aqua-dest as extractor</em><em>.</em><strong><em> </em></strong><em>The subjects</em><strong><em> </em></strong><em>were nine male Sprague-Dawley rats</em><em> devided in to t</em><em>hree</em><em> group </em><em>(negative control, positive control and  experimental). Every group consist of</em><strong><em> </em></strong><em>three</em><em> rats. Heated metal plat (d=</em><em>2</em><em>cm) used to get full thickness burn wound on dorsal rat part. Then, </em><em>Tithonia difersifolia<strong> </strong>folium</em><em> extract </em><em>was given to wound healing(plannimetri method) for 14</em><em> </em><em>days, negative control(aqua dest) 26 days and positive control (Bioplasenton) 20 days.  The results with analysis Anova showed that </em><em>Tithonia difersifolia<strong> </strong>folium</em><em> extract </em><em>have significant</em><strong><em> </em></strong><em>effect (p </em><em>&lt;</em><em> 0,05) to wound healing of rat models. </em></p><p><em> </em></p>

Adipose-Derived Stem Cell Condition Medium Enhances Expression of Ephrin-B2 Related to Neovascularization in Burn Wound Tissue

2018 ◽  
Vol 24 (8) ◽  
pp. 6168-6172
Author(s):  
Elvin Clara Angmalisang ◽  
Dewi Sukmawati ◽  
Lia Damayanti ◽  
Astheria Eryani ◽  
Jeanne A Pawitan
Keyword(s):  
Wound Healing ◽  
Conditioned Medium ◽  
Granulation Tissue ◽  
Skin Wound ◽  
Full Thickness ◽  
Sprague Dawley ◽  
Burn Wound ◽  
Burn Wounds ◽  
Burn Wound Healing ◽  
Wound Tissue

The transmembrane ligand ephrin-B2 is molecular marker for arterial endothelial cells and early blood vessel formation. Neovascularization is one of the important event that support wound healing. Adiposederived stem cells conditioned medium (ADSC-CM) is known to contain cytokines and growth factors as regenerative agent. This study aims to investigate the effect of ADSC-CM on ephrin-B2 expression that represent as neovascularization marker in burn wound granulation tissue. Male Sprague dawley rats were randomly divided into control, conditioned medium (CM) and medium complete (MC) groups. Full-thickness burn wounds were created by placing preheated metal plat on dorsal thorax of the prepared rats. The burn wounds were treated once daily according to their group. Skin wound tissues were collected at day 7, 14, and 21 post wounded for histological analysis. The expression of ephrin-B2 was demonstrated by immunohistochemistry staining. Neovascularization evaluation was done by calculating the number of newly formed capillaries that expressed Ephrin-B2 in wound granulation tissues. Expression of Ephrin-B2 in burn wound tissue was prominently increased in CM compared to control and MC groups on day 7, 14 and 21 post wounded. The CM group also showed higher neovascular number in granulation tissue compared to control and MC group on day 7, 14 and 21 (p < 0.05). Our results showed prominent ephrin-B2 expression in burn wound tissue after treated with ADSC-CM. Topical application of ADSC-CM has shown to promote angiogenesis in full-thickness burn wound in part through the increased of ephrin-B2 expression. Therefore ADSC-CM could act as an alternative strategy to promote burn wound healing.

556 The Use of a Concentrated Surfactant Based Gel to Promote Burn Wound Healing

2021 ◽  
Vol 42 (Supplement_1) ◽  
pp. S126-S127
Author(s):  
Rebecca Coffey ◽  
Rachel Penny
Keyword(s):  
Wound Healing ◽  
New Product ◽  
Ease Of Use ◽  
Burn Injuries ◽  
Full Thickness ◽  
Burn Wound ◽  
Burn Wounds ◽  
Burn Care ◽  
Product Trial ◽  
Burn Wound Healing

Abstract Introduction Strategies to remove necrotic tissue from burn wounds include excisional and non-excisional debridement. Alternative treatments could promote burn wound healing while minimizing patient discomfort and the need for surgery. We evaluated the usage of a concentrated surfactant gel (CSG) to promote burn wound healing in those with indeterminate depth and full thickness burn injuries. Methods An IRB approved retrospective study was conducted during a 10-patient new product trial period with enrollment between September and October 2019. Patients included in this study had indeterminate or full thickness burn wounds and were treated with a concentrated surfactant-based gel. Patients with non-burn diagnoses were excluded. Data collected included demographic information, injury descriptors, and additional burn wound characteristics. Results A total of 10 patients were included in this study as part of a new product trial. The subjects were 80% male with an average TBSA of 7.5%. 40% had indeterminate and 60% had full thickness burn wounds. Prior to initiation of the CSG, the burn wounds had been open for an average of 41 days. There were no infections or complications with usage of the CSG. 90% of patients reported less pain than the standard of care topical agents for burns. Average duration of treatment with the CSG until healing was 28 days. After usage of the CSG, no patients required surgery. Conclusions Our findings support the usage of a concentrated surfactant-based gel in patients with burn wounds. Patients reported decreased pain during dressing changes and ease of use compared to the standard topical agent in burn care. It also prevented surgical debridement in those with indeterminate and full thickness burn injuries.

Development and Characterization of Squalene-Loaded Topical Agar-Based Emulgel Scaffold: Wound Healing Potential in Full-Thickness Burn Model

2020 ◽  
pp. 153473462092162
Author(s):  
T. S. Shanmugarajan ◽  
N. Kalai Selvan ◽  
Varuna Naga Venkata Arjun Uppuluri
Keyword(s):  
Wound Healing ◽  
Tissue Regeneration ◽  
Macrophage Polarization ◽  
Negative Control ◽  
Skin Tissue ◽  
Self Healing ◽  
Full Thickness ◽  
Burn Wound ◽  
Burn Wound Healing ◽  
Tissue Defects

Full-thickness burns pose a major challenge for clinicians to handle because of their restricted self-healing ability. Even though several approaches have been implemented for repairing these burnt skin tissue defects, all of them had unsatisfactory outcomes. Moreover, during recent years, skin tissue engineering techniques have emerged as a promising approach to improve skin tissue regeneration and overcome the shortcomings of the traditional approaches. Although previous literatures report the wound healing effects of the squalene oil, in the current study, for the first time, we developed a squalene-loaded emulgel-based scaffold as a novel approach for potential skin regeneration. This squalene-loaded agar-based emulgel scaffold was fabricated by using physical cross-linking technique using lecithin as an emulsifier. Characterization studies such as X-ray diffraction, Fourier-transform infrared spectroscopy, and field emission scanning electron microscopy revealed the amorphous nature, chemical interactions, and cross-linked capabilities of the developed emulgel scaffold. The squalene-loaded emulgel scaffold showed excellent wound contraction when compared with the agar gel and negative control. In case of the histopathology and recent immunohistochemistry findings, it was clearly evidenced that squalene-loaded emulgel promoted faster rate of the revascularization and macrophage polarization in order to enhance the burn wound healing. Moreover, the findings also revealed that the incorporation of squalene oil into the formulation enhances collagen deposition and accelerates the burnt skin tissue regeneration process. Finally, we conclude that the squalene-loaded emulgel scaffold could be an effective formulation used in the treatment of the burnt skin tissue defects.

scholarly journals Evaluation of the Healing Effects of Hypericum perforatum and Curcumin on Burn Wounds in Rats

2020 ◽  
Vol 2020 ◽  
pp. 1-5
Author(s):  
Nevra Seyhan
Keyword(s):  
Wound Healing ◽  
Hypericum Perforatum ◽  
Control Group ◽  
Sprague Dawley ◽  
Burn Wound ◽  
Burn Wounds ◽  
Burn Wound Healing ◽  
Significant Difference ◽  
Group A ◽  
Second Degree

Background. For centuries, medicinal plants have been extensively used in wound healing of burn injuries. The aim of this study is to analyze comparatively the effects of curcumin and Hypericum perforatum (HP) on second-degree burn wounds in rats. Materials and Methods. This experimental study was conducted on 24 male Sprague-Dawley rats with second-degree burns. The animals were randomly divided into three groups. The burns were treated with curcumin (Group B) and Hypericum perforatum (Group C) with daily application. Group A was considered as the control group and received no medication. Histological parameters, reepithelization, granulation tissue formation, inflammation, and angiogenesis were assessed after the scar biopsy at the end of research. Results. All histological parameters of the control group showed statistically significant difference than the other groups (p<0.05). There was no statistically significant difference between Groups B and C in terms of reepithelization and inflammation (p=0.351, p=0.067). In the curcumin group compared with the HP group, a significant difference was observed for two parameters of fibrosis and angiogenesis (p<0.05). Conclusion. Both curcumin and Hypericum perforatum oil are effective in burn wound healing. Our findings showed a better quality of healing in curcumin-treated rats.

scholarly journals Ozonated Aloe vera Oil Effective Increased the Number of Fibroblasts and Collagen Thickening in the Healing Response of Full-Thickness Skin Defects

2021 ◽  
Vol 2021 ◽  
pp. 1-8
Author(s):  
Ahsanu Taqwim Hidayat ◽  
Muhamad Thohar Arifin ◽  
Muhammad Nur ◽  
Muflihatul Muniroh ◽  
Neni Susilaningsih
Keyword(s):  
Wound Healing ◽  
Aloe Vera ◽  
Full Thickness ◽  
Sprague Dawley ◽  
Control Groups ◽  
Treatment Groups ◽  
A Value ◽  
Healing Response ◽  
Sprague Dawley Rats ◽  
Significant Difference

Objective. This study aimed to examine the effectiveness of ozonated Aloe vera oil on the wound healing response of full-thickness defect tissue in Sprague-Dawley rats, assessed by collagen thickness and the number of fibroblasts. Methods. This was an experimental research method using control groups and treatment groups with a posttest only control group design. The results showed that collagen thickness in wounds tended to increase, assessed on day 3 and day 7 using Masson’s trichrome staining and microscopic evaluation. Results. There was a significant difference in the number of fibroblasts between the two control and treatment groups on days 3 and 7 tested using one-way Kruskal–Wallis test, with a value of p = 0.001 p < 0.05 , resulting in a significant difference in wound size reduction between the groups. Further post hoc analysis using the Mann–Whitney test indicated a significant difference between the control groups and the treatment groups (P0, P1 versus P3, P4, P5, P8, P9, and P10) with a value of p = 0.009 p < 0.05 . Conclusions. Ozonated Aloe vera oil is effective in increasing the healing response of full-thickness defects, leading to the increase in the number of fibroblasts and collagen thickening that in turn accelerates wound healing in Sprague-Dawley rats.

scholarly journals AUTOLOGOUS FULL THICKNESS SKIN GRAFT WOUND HEALING ON SPRAGUE DAWLEY RATS Reviewed From TGF-? Expression And Neutrophil Number

2021 ◽  
Vol 8 (3) ◽  
pp. 317-325
Author(s):  
Nauval Marta Kusuma ◽  
Hardian Hardian ◽  
Najatullah Najatullah ◽  
Renni Yuniati ◽  
Neni Susilaningsih
Keyword(s):  
Wound Healing ◽  
Skin Graft ◽  
Control Group ◽  
Full Thickness ◽  
Sprague Dawley ◽  
Full Thickness Skin Graft ◽  
Neutrophil Number ◽  
Full Thickness Skin ◽  
Sprague Dawley Rats ◽  
Thickness Skin

Abstract Introduction : Skin grafts are now one of treatment option in wound healing process that is always developing. TGF-bexpression and the number of neutrophils have an important role in healing skin graft wounds. Ozone (O3) has disinfecting properties that are effective in wound healing. Objective : Proving the effectiveness of Ozonated VCO for Full Thickness Skin Graft wound healing using parameter of TGF-b expression and neutrophil number. Method : This study is an experimental study with a post-test only design group of 40 Sprague Dawley rats performed autologous skin graft at the same time. Samples were divided randomly into 8 groups (K1 = without Ozonated VCO), (A1 = Ozonated VCO 50 mg / ml), (B1 = Ozonated VCO 100 mg / ml), (C1 = Ozonated VCO 200 mg / ml), ( K2 = without Ozonated VCO) (A2 = Ozonated VCO 50 mg / ml), (B2 = Ozonated VCO 100 mg / ml), (C2 = Ozonated VCO 200 mg / ml). Assessment of TGF-b expression and neutrophil number of tissue was performed by staining hematoxylin & eosin and immunohistochemistry on days 6 and 12 after skin graft. Results : There were significant differences (p <0.05) TGF-b expression and neutrophils number of tissue between the control group and the administration of Ozonated VCO doses of 50 mg / ml, 100 mg / ml and 200 mg / ml on days 6 and 12 post skin graft. Conclusion : The administration of Ozonated VCO effectively improve Full Thickness Skin Graft wound healing seen from macroscopic wounds, increase TGF-b expression and decrease the number of neutrophils.

scholarly journals Ankaferd blood stopper accelerates deep second degree burn wound healing in rats

2018 ◽  
Vol 87 (3) ◽  
pp. 261-267 ◽  
Author(s):  
Ayse Topal ◽  
Nihal Y. Gul Satar ◽  
I. Taci Cangul ◽  
M. Ayberk Oktay ◽  
Kivanc Inan ◽  
...  
Keyword(s):  
Wound Healing ◽  
Histopathological Examination ◽  
Wound Contraction ◽  
Control Group ◽  
Sprague Dawley ◽  
Burn Wound ◽  
Burn Wound Healing ◽  
Degree Burn ◽  
Ankaferd Blood Stopper ◽  
The Mean

In this study, the effects of Ankaferd Blood Stopper (ABS) and silver sulphadiazine (SSD) cream on burn wound healing were investigated in rats. A total of 24 outbred, male, Sprague-Dawley rats were randomly allocated to (1) ABS, (2) SSD, and (3) control groups. Bilateral burn wounds were created near the caudal border of the scapula. Wounds in each group were treated daily with sponges soaked in ABS solution, 1% SSD cream, or saline, respectively. On days 0, 7, 14, 21, and 28, unhealed wound area was measured and biopsy samples were taken for histopathological examination (except day 0). At the end of day 28, all rats in the ABS and SSD groups had complete coverage of the wounds with granulation tissue and epithelialization, whereas wounds in the control group were not completely epithelialized. On day 7, the mean unhealed wound areas and the mean percentages of wound contraction were not significantly different among the groups. However, the mean percentage of wound contraction in the ABS and SSD groups was significantly higher than in the control group on days 14, 21, and 28. Histopathologically, wound healing was characterized by a decrease in neutrophil counts and an increase in vessel counts. Our results suggest that ABS can be successfully used for burn wound healing besides SSD.

scholarly journals The Superior Outcome of Collagen Nanosilver in Increasing Macrophage and Collagen on Deep Dermal Burn Wound Healing

2022 ◽  
Vol 10 (B) ◽  
pp. 31-35
Author(s):  
Anggun Ari Mukti ◽  
Awal Prasetyo ◽  
Yan Wisnu Prajoko ◽  
Najatullah Najatullah ◽  
Neni Susilaningsih
Keyword(s):  
Wound Healing ◽  
Positive Control ◽  
Negative Control ◽  
Group Iv ◽  
Sprague Dawley ◽  
Burn Wound ◽  
Group Iii ◽  
Burn Wound Healing ◽  
Significant Difference ◽  
Collagen Density

AIM: This study aims to prove the superior outcome of collagen nanosilver in increasing macrophage and collagen on deep dermal burn wound healing. METHODS: This study was a randomized control trial design. All animals used were male Sprague Dawley rats aged 12–14 weeks old and weighed about 200–250 g. Each rat gets four site injuries and is given treatments. The treatment group includes: I (negative control using NaCl 0.9%), II (positive control using silver sulfadiazine cream), III (treatment with nanosilver collagen cream 1%), and IV (treatment with collagen nanosilver cream 2%). Macrophages counts were assessed using IHC F40/80 and collagen density was assessed using Masson’s trichrome staining. RESULTS: Macrophage on day 6 showed a significant difference as seen in group III and group IV. A significant difference was also obtained between macrophage on day 3 and day 6 from group IV, likewise between collagen between day 3 and day 6 from group III and IV. Collagen nanosilver acts as an antimicrobial and anti-inflammatory agent by increasing the mechanism of activation and migration of macrophages to the site of injury. Moreover, this formula modulated the collagen deposition, which forms the tissue fibers which ultimately provide a conducive environment for wound healing. CONCLUSION: Collagen nanosilver has a superior outcome in the increasing macrophage cell count and collagen density of deep dermal burn wound healing.

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