scholarly journals PENGARUH INFUSA DAUN KEMBANG BULAN (Tithonia difersifolia) PADA KECEPATAN PENYEMBUHAN LUKA BAKAR

2018 ◽  
Vol 12 (3) ◽  
pp. 230
Author(s):  
Dwi Mulyani
Keyword(s):  
Wound Healing ◽  
Positive Control ◽  
Negative Control ◽  
Full Thickness ◽  
Sprague Dawley ◽  
Burn Wound ◽  
Rat Models ◽  
Sprague Dawley Rats ◽  
Healing Wound ◽  
Heated Metal

<p><em>The aim of this investigation was a clinical</em><em> </em><em>evaluation of the</em><em> </em><em>accelerating effect of </em><em>Tithonia difersifolia </em><em>extract<strong> </strong></em><strong><em> </em></strong><em>on the burn </em><a title="Find more articles at http://www.scialert.net/asci/result.php?searchin=Keywords&amp;cat=&amp;ascicat=ALL&amp;Submit=Search&amp;keyword=wound+healing (wound healing)" href="http://www.scialert.net/asci/result.php?searchin=Keywords&amp;cat=&amp;ascicat=ALL&amp;Submit=Search&amp;keyword=wound+healing" target="_blank"><em>wound healing</em></a><em> in male Sprague-Dawley rats weighted 20</em><em>0</em><em>-</em><em>3</em><em>5</em><em>0</em><em>.</em><em> T</em><em>he  sample then extracted using wet rendering (infusa) method using aqua-dest as extractor</em><em>.</em><strong><em> </em></strong><em>The subjects</em><strong><em> </em></strong><em>were nine male Sprague-Dawley rats</em><em> devided in to t</em><em>hree</em><em> group </em><em>(negative control, positive control and  experimental). Every group consist of</em><strong><em> </em></strong><em>three</em><em> rats. Heated metal plat (d=</em><em>2</em><em>cm) used to get full thickness burn wound on dorsal rat part. Then, </em><em>Tithonia difersifolia<strong> </strong>folium</em><em> extract </em><em>was given to wound healing(plannimetri method) for 14</em><em> </em><em>days, negative control(aqua dest) 26 days and positive control (Bioplasenton) 20 days.  The results with analysis Anova showed that </em><em>Tithonia difersifolia<strong> </strong>folium</em><em> extract </em><em>have significant</em><strong><em> </em></strong><em>effect (p </em><em>&lt;</em><em> 0,05) to wound healing of rat models. </em></p><p><em> </em></p>

scholarly journals EFFECT OF THE WATER EXTRACT OF THE FOUR O’CLOCK HERB (MIRABILIS JALAPA L.) ON THE HEALING OF OPEN WOUNDS IN RATS

2018 ◽  
Vol 10 (1) ◽  
pp. 155
Author(s):  
Puspita Puspasari ◽  
Fadlina Chany Saputri
Keyword(s):  
Wound Healing ◽  
Water Extract ◽  
Optimal Dose ◽  
Positive Control ◽  
Negative Control ◽  
Sprague Dawley ◽  
Sprague Dawley Rats ◽  
Mirabilis Jalapa ◽  
Hematoxylin And Eosin ◽  
Iodine Dose

Objective: This study aimed to analyze the effect on wound healing following treatment with a water extract of Mirabilis jalapa L. by investigatingwound contraction and the associated histopathological changes in rat skin.Methods: Male Sprague-Dawley rats were divided into five groups, namely negative control, positive control (povidone-iodine), dose 1, dose 2, anddose 3. A 20-×10-mm rectangular wound area was created for the test. In dose 1, 2, and 3 groups, the corresponding dose variation of a 0.5-mLM. jalapa L. water extract (dose 1: 5% v/v, ≈243.1 mg/kg body weight BW; dose 2: 10% v/v, ≈486.2 mg/kg BW; and dose 3: 20% v/v, ≈972.4 mg/kg BW)was topically applied for 14 days on open wounds of rats. Widespread wound contractions were measured on days 1, 3, 5, 7, 9, 11, and 13, andhistopathological changes in the skin were observed on day 15 using hematoxylin and eosin staining.Results: The M. jalapa L. water extract accelerated wound healing. The optimal dose was found to be 20% v/v (≈972.4 mg/kg BW).Conclusion: M. jalapa L. extracts are potential healing agents for open wounds.

scholarly journals The Superior Outcome of Collagen Nanosilver in Increasing Macrophage and Collagen on Deep Dermal Burn Wound Healing

2022 ◽  
Vol 10 (B) ◽  
pp. 31-35
Author(s):  
Anggun Ari Mukti ◽  
Awal Prasetyo ◽  
Yan Wisnu Prajoko ◽  
Najatullah Najatullah ◽  
Neni Susilaningsih
Keyword(s):  
Wound Healing ◽  
Positive Control ◽  
Negative Control ◽  
Group Iv ◽  
Sprague Dawley ◽  
Burn Wound ◽  
Group Iii ◽  
Burn Wound Healing ◽  
Significant Difference ◽  
Collagen Density

AIM: This study aims to prove the superior outcome of collagen nanosilver in increasing macrophage and collagen on deep dermal burn wound healing. METHODS: This study was a randomized control trial design. All animals used were male Sprague Dawley rats aged 12–14 weeks old and weighed about 200–250 g. Each rat gets four site injuries and is given treatments. The treatment group includes: I (negative control using NaCl 0.9%), II (positive control using silver sulfadiazine cream), III (treatment with nanosilver collagen cream 1%), and IV (treatment with collagen nanosilver cream 2%). Macrophages counts were assessed using IHC F40/80 and collagen density was assessed using Masson’s trichrome staining. RESULTS: Macrophage on day 6 showed a significant difference as seen in group III and group IV. A significant difference was also obtained between macrophage on day 3 and day 6 from group IV, likewise between collagen between day 3 and day 6 from group III and IV. Collagen nanosilver acts as an antimicrobial and anti-inflammatory agent by increasing the mechanism of activation and migration of macrophages to the site of injury. Moreover, this formula modulated the collagen deposition, which forms the tissue fibers which ultimately provide a conducive environment for wound healing. CONCLUSION: Collagen nanosilver has a superior outcome in the increasing macrophage cell count and collagen density of deep dermal burn wound healing.

Ranitidine as an alcohol dehydrogenase inhibitor in acute methanol toxicity in rats

2009 ◽  
Vol 29 (2) ◽  
pp. 93-101 ◽  
Author(s):  
Amal A El-Bakary ◽  
Sahar A El-Dakrory ◽  
Sohayla M Attalla ◽  
Nawal A Hasanein ◽  
Hala A Malek
Keyword(s):  
Alcohol Dehydrogenase ◽  
High Performance ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Sprague Dawley ◽  
Blood Samples ◽  
Methanol Poisoning ◽  
Sprague Dawley Rats

Methanol poisoning is a hazardous intoxication characterized by visual impairment and formic acidemia. The therapy for methanol poisoning is alcohol dehydrogenase (ADH) inhibitors to prevent formate accumulation. Ranitidine has been considered to be an inhibitor of both gastric alcohol and hepatic aldehyde dehydrogenase enzymes. This study aimed at testing ranitidine as an antidote for methanol acute toxicity and comparing it with ethanol and 4-methyl pyrazole (4-MP). This study was conducted on 48 Sprague-Dawley rats, divided into 6 groups, with 8 rats in each group (one negative control group [C1], two positive control groups [C2, C3] and three test groups [1, 2 and 3]). C2, C3 and all test groups were exposed to nitrous oxide by inhalation, then, C3 group was given methanol (3 g/kg orally). The three test groups 1, 2 and 3 were given ethanol (0.5 g/kg orally), 4-MP (15 mg/kg intraperitoneally) and ranitidine (30 mg/kg intraperitoneally), respectively, 4 hours after giving methanol. Rats were sacrificed and heparinized, cardiac blood samples were collected for blood pH and bicarbonate. Non-heparinized blood samples were collected for formate levels by high performance liquid chromatography. Eye balls were enucleated for histological examination of the retina. Ranitidine corrected metabolic acidosis (p = .025), decreased formate levels (p = .014) and improved the histological findings in the retina induced by acute methanol toxicity.

scholarly journals PENGARUH SINBIOTIK KEFIR PISANG BATU TERHADAP KADAR KOLESTEROL-LDL DAN KOLESTEROL-HDL TIKUS MODEL SINDROM METABOLIK

2020 ◽  
Vol 7 (2) ◽  
pp. 280-288
Author(s):  
Dina Khoiriyah ◽  
Taufik Maryusman ◽  
Santi Herlina
Keyword(s):  
Risk Factors ◽  
Metabolic Syndrome ◽  
Ldl Cholesterol ◽  
Hdl Cholesterol ◽  
Positive Control ◽  
Negative Control ◽  
Sprague Dawley ◽  
Musa Balbisiana ◽  
Cholesterol Levels ◽  
Sprague Dawley Rats

Effect of Banana Kefir Synbiotic on LDL-Cholesterol and HDL-Cholesterol of Metabolic Syndrome Rats Metabolic syndrome (SM) is characterized by several risk factors including dyslipidemia. This study aims to analyze the effect of kefir synbiotic produced from banana stone flour (Musa balbisiana) on LDL-cholesterol and HDL-cholesterol of metabolic syndrome rat model. The 24 Sprague Dawley rats were divided into four groups, namely negative control (healthy rats fed standard feed), positive control (metabolic syndrome rats fed standard feed), treatment I and treatment II (metabolic syndrome rats each given synbiotic kefir banana stone flour 1.8 mL/200 g mouse BW/day and 3.6 mL/200 g mouse BW/day, respectively). The intervention was carried out for three weeks. After the intervention, the levels of LDL-cholesterol in treatment I and II experienced a decrease of 44.66% and 56.94%, respectively, while the-HDL-cholesterol levels in treatment I and II experienced an increase of 104.5% and 172.71%, respectively. The biggest change occurred in treatment II. Synbiotic kefir banana stone flour improved lipid profile in metabolic syndrome rats. Sindrom metabolik (SM) ditandai dengan beberapa faktor risiko termasuk dislipidemia. Penelitian ini bertujuan untuk menganalisis pengaruh sinbiotik kefir tepung pisang batu (Musa balbisiana) terhadap kadar kolesterol-LDL dan kolesterol-HDL tikus model SM. Subjek menggunakan 24 ekor tikus Sprague Dawley yang dibagi menjadi empat kelompok, yaitu kontrol negatif (tikus sehat yang diberi pakan standar), kontrol positif (tikus model SM yang diberi pakan standar), perlakuan I dan perlakuan II (tikus model SM yang masing-masing diberi sinbiotik kefir tepung pisang batu 1,8 mL/200 g BB tikus/hari dan 3,6 mL/200 g BB tikus/hari). Proses intervensi dilakukan selama tiga minggu. Setelah intervensi, kadar kolesterol-LDL perlakuan I dan II mengalami penurunan sebesar 44,66% dan 56,94%, sedangkan kadar kolesterol-HDL perlakuan I dan II mengalami peningkatan sebesar 104,5% dan 172,71%. Perubahan terbesar terjadi pada perlakuan II. Sinbiotik kefir tepung pisang batu memperbaiki profil lipid tikus sindrom metabolik.

Buprenorphine alleviation of pain does not compromise the rat monoarthritic pain model

2017 ◽  
Vol 16 (1) ◽  
pp. 167-167
Author(s):  
M.S. Berke ◽  
Klas S.P. Abelson
Keyword(s):  
Rat Model ◽  
Joint Stiffness ◽  
Positive Control ◽  
Negative Control ◽  
Pain Tolerance ◽  
Control Group ◽  
Mechanical Stimuli ◽  
Sprague Dawley ◽  
Sprague Dawley Rats ◽  
A Minor

Abstract Aims This study investigated the effects of buprenorphine treatment on pain and welfare parameters and model specific parameters in a rat model of monoarthritis to eliminate unnecessary pain from this model. Methods 32 male Sprague Dawley rats were divided into four groups: (1) A negative control without arthritis receiving no analgesia. (2) A positive monoarthritic control group receiving no analgesia, but subcutaneous saline injections twice a day. (3) A positive control with monoarthritis receiving subcutaneous carprofen once a day and saline once a day. (4) A group with monoarthritis receiving subcutaneous buprenorphine twice a day. Monoarthritis was induced with an injection of 0.02 ml Complete Freund’s Adjuvant intra-articularly in the left tibiotarsal joint. Treatment with analgesia was initiated at day 15 and the rats were euthanized at day 23. Results The induced monoarthritis elicited a pronounced acute inflammation. Several parameters such as bodyweight, mobility, stance, joint-stiffness and lameness scores were affected. A marked mechanical hyperalgesia in the tarsal area was observed by Electronic Von Frey testing, but no severe compromise of the animal welfare was seen at any time. Signs of chronic development began to appear from day 10 after the monoarthritic induction. No significant change in serum cytokines and faecal corticosterone measurements was found after administration of buprenorphine. A minor decrease in body weight was seen, and a higher pain tolerance to mechanical stimuli was observed, indicating pain alleviation. The histological examination confirmed monoarthritic development in all monoarthritic rats and revealed periarticular lesions suggesting diffusion of adjuvant from intra-articular injection site to the periphery. Conclusions The study demonstrated that buprenorphine has an analgesic effect in the adjuvant induced monoarthritic rat model, without obvious interference with the development of arthritis.

Development and Characterization of Squalene-Loaded Topical Agar-Based Emulgel Scaffold: Wound Healing Potential in Full-Thickness Burn Model

2020 ◽  
pp. 153473462092162
Author(s):  
T. S. Shanmugarajan ◽  
N. Kalai Selvan ◽  
Varuna Naga Venkata Arjun Uppuluri
Keyword(s):  
Wound Healing ◽  
Tissue Regeneration ◽  
Macrophage Polarization ◽  
Negative Control ◽  
Skin Tissue ◽  
Self Healing ◽  
Full Thickness ◽  
Burn Wound ◽  
Burn Wound Healing ◽  
Tissue Defects

Full-thickness burns pose a major challenge for clinicians to handle because of their restricted self-healing ability. Even though several approaches have been implemented for repairing these burnt skin tissue defects, all of them had unsatisfactory outcomes. Moreover, during recent years, skin tissue engineering techniques have emerged as a promising approach to improve skin tissue regeneration and overcome the shortcomings of the traditional approaches. Although previous literatures report the wound healing effects of the squalene oil, in the current study, for the first time, we developed a squalene-loaded emulgel-based scaffold as a novel approach for potential skin regeneration. This squalene-loaded agar-based emulgel scaffold was fabricated by using physical cross-linking technique using lecithin as an emulsifier. Characterization studies such as X-ray diffraction, Fourier-transform infrared spectroscopy, and field emission scanning electron microscopy revealed the amorphous nature, chemical interactions, and cross-linked capabilities of the developed emulgel scaffold. The squalene-loaded emulgel scaffold showed excellent wound contraction when compared with the agar gel and negative control. In case of the histopathology and recent immunohistochemistry findings, it was clearly evidenced that squalene-loaded emulgel promoted faster rate of the revascularization and macrophage polarization in order to enhance the burn wound healing. Moreover, the findings also revealed that the incorporation of squalene oil into the formulation enhances collagen deposition and accelerates the burnt skin tissue regeneration process. Finally, we conclude that the squalene-loaded emulgel scaffold could be an effective formulation used in the treatment of the burnt skin tissue defects.

scholarly journals Evaluations of bacterial contaminated full thickness burn wound healing in Sprague Dawley rats Treated with Tualang honey

2011 ◽  
Vol 44 (01) ◽  
pp. 112-117 ◽  
Author(s):  
Salmi Mohamed Sukur ◽  
Ahmad Sukari Halim ◽  
Kirnpal Kaur Banga Singh
Keyword(s):  
Wound Healing ◽  
Full Thickness ◽  
Sprague Dawley ◽  
Burn Wounds ◽  
Wound Size ◽  
Sprague Dawley Rats ◽  
Study Results ◽  
The Mean ◽  
Tualang Honey ◽  
Chitosan Gel

ABSTRACT Aim: The effect of Tualang honey on wound healing in bacterial contaminated full-thickness burn wounds was evaluated in 36 male Sprague Dawley rats. Materials and Methods: The rats were randomly divided into three groups (n = 12/group). Three full-thickness burn wounds were created on each rat. Each group of rats was inoculated with a different organism in the burn wounds: Group A was inoculated with Pseudomonas aeruginosa, Group B was inoculated with Klebsiella pneumoniae and Group C was inoculated with Acinetobacter baumannii. One wound on each rat was dressed with either Tualang honey, Chitosan gel or Hydrofibre silver. Each wound size was measured on day 3, 6, 9, 12, 15, 18 and 21 of the study. Results: The mean wound size of the Tualang honey-treated wounds was not statistically different than that of the Chitosan gel or Hydrofibre silver-treated wounds when the wounds were compared throughout the entire experiment (P > 0.05). However, comparing the mean wound size on day 21 alone revealed that the Tualang honey-treated wounds were smaller in comparison to that of the Chitosan gel and Hydrofibre silver-treated groups. Conclusions: This study shows that topical application of Tualang honey on burn wounds contaminated with P. aeruginosa and A. baumannii gave the fastest rate of healing compared with other treatments.

scholarly journals The Effect ofCamellia sinensison Wound Healing Potential in an Animal Model

2013 ◽  
Vol 2013 ◽  
pp. 1-7 ◽  
Author(s):  
Fatemeh Hajiaghaalipour ◽  
M. S. Kanthimathi ◽  
Mahmood Ameen Abdulla ◽  
Junedah Sanusi
Keyword(s):  
Wound Healing ◽  
Camellia Sinensis ◽  
Inflammatory Cells ◽  
Positive Control ◽  
Sprague Dawley ◽  
Wound Area ◽  
Sprague Dawley Rats ◽  
Scar Width ◽  
Uniform Area ◽  
Wound Healing Activity

Camellia sinensis(tea) is reported to have health benefits, including the building of healthy skin. This study evaluated the effects of topical application ofCamellia sinensisextract on the rate of wound closure and the histology of wound area. A uniform area of 2.00 cm in diameter was excised from the neck of adult male Sprague Dawley rats. The animals were topically treated with 0.2 mL of vehicle (CMC), Intrasite gel (positive control), or 200 and 400 mg/mL of extract. Wounds dressed with the extract and Intrasite gel healed significantly earlier than those with vehicle. Histological analysis of the wound area after 10 days showed that wounds dressed with the extract had less scar width when compared to the control. The tissue contained less inflammatory cells and more collagen and angiogenesis, compared to wounds dressed with vehicle. In this study,Camellia sinensisshowed high potential in wound healing activity.

scholarly journals Freeze Dried extracts of Bidens biternata (Lour.) Merr. And Sheriff. show significant Antidiarrheal activity in ‐vivo Models of Diarrhea

2016 ◽  
Author(s):  
Dennis Gacigi Kinuthia ◽  
Anne W. Muriithi ◽  
Peter Waweru Mwangi
Keyword(s):  
Normal Saline ◽  
Castor Oil ◽  
Contractile Activity ◽  
Positive Control ◽  
Negative Control ◽  
Sprague Dawley ◽  
Sprague Dawley Rats ◽  
Freeze Dried ◽  
Adrenergic Blocker

ABSTRACTEthnopharmacological relevance of the studyDiarrhea remains one of the main killers of children aged below five years. Traditional antidiarrheal remedies form a potentially viable source of novel low cost efficacious antidiarrheal remedies in low resource settings. There is therefore a pressing to scientifically evaluate these remedies.Aim of the studyThis study aimed to investigate the in vivo and in vitro antidiarrheal activity of Bidens biternata a herb species used in traditional Ayurvedic medicine in the management of diarrhea.Materials and MethodsIn the castor oil test twenty (20) adult Sprague-Dawley rats were randomized to the negative control (normal saline), positive control (5 mg/kg loperamide), (200 mg/kg Bidens biternata extract) and (400 mg/kg Bidens biternata extract) groups (n=5 in each group). Castor oil (4 ml/kg) was then administered to the animals one hour after administration of the respective treatments after which the total mass of fecal output excreted after four (4) hours was determined.In the charcoal meal test fifteen (15) Sprague Dawley rats were randomized to the negative control (normal saline 5 ml/kg orally), the positive control (atropine sulphate 0.1 mg/kg i.p) and test (400 mg/kg Bidens biternata extract) groups (n=5). Charcoal meal was then administered via oral gavage to each rat thirty (30) minutes after the administration of the various treatments. The distance covered by the charcoal meal from the pylorus was then determined after sacrifice of the animals.In the enteropooling test twenty (20) Sprague-Dawley rats were randomized to the negative control (5% v/v ethanol in normal saline), positive control (5 mg/kg loperamide) and test (400 mg/kg Bidens biternata extract) groups and prostaglandin E2 (PGE2) (100μg/kg) administered immediately after the treatments. The animals were then sacrificed half an hour later and the volume of the small intestine contents determined. The effects of different concentrations of Bidens biternata extract (0.5. 1.0, 2.0, 3.0 and 5.0 mg/ml) on jejunal contraction were investigated and a dose-response curve constructed using the experimental data after which The ED50 dose determined. The effect of tamsulosin (α1 adrenergic blocker), yohimbine (α2 adrenergic blocker), propranolol (β adrenergic blocker) and naloxone (μ opioid blocker) on the contractile activity of the extract were also investigated.The experimental data were expressed as mean ± standard error of mean (SEM) and then analyzed using one way ANOVA followed by Tukey’s post hoc test in cases of significance (set at p<0.05).ResultsThe freeze dried extracts of Bidens biternata had significant antidiarrhealeffects in the castor oil induced diarrhea model (p=0.0075) with maximal activity being observed at the 400mg/kg dosage level (1.66± 0.81g vs. 4.54 ± 0.51 g negative control, p=0.01). Bidens biternata extract had significant effects on intestinal motility in the charcoal meal test compared to the control group (43.61 ± 4.42% vs. 60.54 ± 3.33%: p= 0.02). Bidens biternata extract had a significant effect on PGE2 induced enteropooling (3.06 ± 0.07 ml vs. 4.74 ± 0.10 ml; p<0.001).The freeze dried extracts of Bidens biternata had a significant negative effect on the contractility of the isolated rabbit jejunum (p<0.001). The effects of the extract were significantly attenuated by tamsulosin (53.94 ± 4.20% vs. 80.57 ± 4.09%; p=0.0067) and naloxone (53.94 ± 4.20% vs. 73.89 ± 7.26 %; p=0.0358). Yohimbine (p=0.4598) and propranolol (p=0.5966) however did not have any significant effect on the contractile activity of the extract.ConclusionsThe freeze dried extract of Bidens biternata possess significant antidiarrhealactivity in both in vitro and in vivo models which appears to be mediated by modulating both the intestinal motility as well as the secretory activity. The results of this study also validate its traditional use as an antidiarrheal remedy.

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