scholarly journals Histochemical detection and comparison of apoptotic cells in the gingival epithelium using hematoxylin and eosin and methyl green-pyronin: A pilot study

2016 ◽  
Vol 0 (0) ◽  
pp. 0
Author(s):  
Anita Raikar ◽  
Aarati Nayak ◽  
Vijaylaxmi Kotrashetti ◽  
Ramakant Nayak ◽  
Sumedha Shree ◽  
...  
Keyword(s):  
Pilot Study ◽  
Methyl Green ◽  
Apoptotic Cells ◽  
Histochemical Detection ◽  
Gingival Epithelium ◽  
Hematoxylin And Eosin

scholarly journals Expression of PCNA in oral gingival epithelium of aggressive and, chronic periodontitis—A pilot study

2013 ◽  
Vol 03 (01) ◽  
pp. 112-117 ◽  
Author(s):  
Penapy Jyostna ◽  
Manchala Sesha Reddy ◽  
Manyam Ravikanth ◽  
Mandalapu Narendra ◽  
Chini Dorasani Dwarakanath
Keyword(s):  
Pilot Study ◽  
Chronic Periodontitis ◽  
Gingival Epithelium ◽  
Oral Gingival Epithelium

1-Octadecene as a Solvent for Ferrofluids for Intraocular use

1997 ◽  
Vol 7 (3) ◽  
pp. 288-293
Author(s):  
P. Tejada ◽  
M.J. Méndez ◽  
S. Madero ◽  
C. Cava ◽  
A. Barcelo ◽  
...  
Keyword(s):  
Double Bond ◽  
Pilot Study ◽  
Gas Exchange ◽  
Inflammatory Response ◽  
Light Microscopy ◽  
Salt Solution ◽  
Cytotoxic Effect ◽  
Gas Injection ◽  
Preliminary Information ◽  
Hematoxylin And Eosin

Purpose. 1-Octadecene is a hydrocarbon with one double bond in its structure that could serve as a solvent for ferrofluids. The aim of this pilot study was to obtain preliminary information on intraocular tolerance to 1-octadecene. Methods. Vitreous compression with perfluoropropane gas was achieved in 20 eyes of albino rabbits. Four days after gas injection a fluid-gas exchange was undertaken. Sixteen eyes received 1-octadecene. Four eyes received balanced salt solution. Eyes were obtained at 3, 7, 14 and 30 days. The samples were fixed in 10% buffered formalin, processed in paraffin and sections were stained with hematoxylin and eosin. Results. Emulsification of the oil bubble was observed in 31.25% of the cases by the fifth day; light microscopy showed normal retinal architecture in all the eyes and epiretinal and vitreous macrophages in 50% of the eyes. Conclusions. 1-Octadecene does not appear to have any retinal cytotoxic effect but elicits an inflammatory response in the vitreous activity.

scholarly journals Brain Morphological Changes in COVID-19

2020 ◽  
Author(s):  
Anastasia S. Babkina ◽  
Arkady M. Golubev ◽  
Irina V. Ostrova ◽  
Alexei V. Volkov ◽  
Artem N. Kuzovlev
Keyword(s):  
Neuronal Damage ◽  
Morphological Changes ◽  
Individual Risk ◽  
Methyl Green ◽  
Neuronal Marker ◽  
Neuronal Nuclei ◽  
Brain Material ◽  
The Central Nervous System ◽  
Hematoxylin And Eosin ◽  
Individual Risk Factors

The aim of the study was to identify the pathomorphology of brain damage in patients who died of COVID-19.Material and methods. Autopsy reports and autopsy brain material of 17 deceased patients with pre-mortem confirmed COVID-19 infection were analyzed. Fatal cases in which COVID19 was the major cause of death were included in the study. Five people were diagnosed with cerebral infarction. Organ samples were taken for histological examination during autopsy. Sections were stained with hematoxylin and eosin and by Nissl to assess brain histopathology. To study the vascular basal membranes the PAS reaction was used, to detect fibrin in vessels phosphotungstic acid-hematoxylin (PTAH) staining was used, to determine DNA in nuclei sections were stained according to Feulgen, to detect RNA in neuronal nuclei and cytoplasm sections were stained with methyl green-pyronin. Immunohistochemical study of a neuronal marker, nuclear protein NeuN, was performed to assess neuronal damage.Results. The signs of neuronal damage found in patients who died of COVID-19 included nonspecific changes of nerve cells (acute swelling, retrograde degeneration, karyolysis and cytolysis, ‘ghost' cells, neuronophagia and satellitosis) and signs of circulatory disorders (perivascular and pericellular edema, diapedesis, congested and engorged microvasculature).Conclusion. Brain histopathological data indicate damage to the central nervous system in COVID-19 patients. Ischemic stroke in patients with COVID-19 is mostly caused by a combination of hypoxia resulting from respiratory failure and individual risk factors, including cerebrovascular atherosclerosis and hypertension.

scholarly journals Localization of Apoptotic Cells in the Human Epidermis by an In Situ DNA Nick End-labeling Method Using Confocal Reflectant Laser Microscopy

1998 ◽  
Vol 46 (6) ◽  
pp. 783-786 ◽  
Author(s):  
Y. Ito ◽  
Y. Otsuki
Keyword(s):  
Human Epidermis ◽  
Methyl Green ◽  
Apoptotic Cells ◽  
Silver Complexes ◽  
Basal Cells ◽  
Granular Cells ◽  
Laser Microscopy ◽  
Double Stranded Dna ◽  
Dna Ends

We describe an immunohistochemical method that allows the detection of apoptotic cells in human epidermis by use of confocal laser reflectance and antibody-immunogold-silver complexes. For this purpose, the site of free 3′-OH DNA ends was detected by the reflectance from heavy metal products (anti-digoxigenin antibody-immunogold-silver complexes) instead of 3, 3′ -diaminobenzidine (DAB) reaction products in the conventional in situ nick end-labeling of DNA strand breaks (ISEL) technique. Localization of double-stranded DNA was demonstrated by the autofluorescence of methyl green. The ISEL technique using confocal reflectant laser microscopy (CRLM) clearly showed the most intense reflectance in the nuclei of granular cells, in contrast to only a weaker reflectance in those of basal cells. On the other hand, the extent of autofluorescence of methyl green was significantly more intense in the nuclei of basal cells and showed a reciprocal relation to that of the reflectance. Therefore, granular cells were most prone to apoptosis and did not contain double-stranded DNA, as indicated by the lack of stainability with methyl green. In addition, this method demonstrating the simultaneous localization of both free 3′-OH DNA ends and double-stranded DNA proved to have a wide range of applications, including the study of other DNA autolytic processes.

scholarly journals Aggregatibacter actinomycetemcomitans Infection Enhances ApoptosisIn Vivothrough a Caspase-3-Dependent Mechanism in Experimental Periodontitis

2012 ◽  
Vol 80 (6) ◽  
pp. 2247-2256 ◽  
Author(s):  
Jun Kang ◽  
Beatriz de Brito Bezerra ◽  
Sandra Pacios ◽  
Oelisoa Andriankaja ◽  
Yu Li ◽  
...  
Keyword(s):  
Bone Loss ◽  
Connective Tissue ◽  
Caspase 3 ◽  
Aggregatibacter Actinomycetemcomitans ◽  
Apoptotic Cells ◽  
Content Type ◽  
Tnf Α ◽  
Gingival Epithelium ◽  
Periodontal Destruction ◽  
Dependent Mechanism

ABSTRACTThe purpose of this study was to test the hypothesis that diabetes aggravates periodontal destruction induced byAggregatibacter actinomycetemcomitansinfection. Thirty-eight diabetic and 33 normal rats were inoculated withA. actinomycetemcomitansand euthanized at baseline and at 4, 5, and 6 weeks after inoculation. Bone loss and the infiltration of polymorphonuclear leukocytes (PMNs) in gingival epithelium were measured in hematoxylin-eosin-stained sections. The induction of tumor necrosis factor alpha (TNF-α) was evaluated by immunohistochemistry and of apoptotic cells by a TUNEL (terminal deoxynucleotidyltransferase-mediated dUTP-biotin nick end labeling) assay. AfterA. actinomycetemcomitansinfection, the bone loss in diabetic rats was 1.7-fold and the PMN infiltration 1.6-fold higher than in normoglycemic rats (P< 0.05). The induction of TNF-α was 1.5-fold higher and of apoptotic cells was up to 3-fold higher in diabetic versus normoglycemic rats (P< 0.05). Treatment with a caspase-3 inhibitor significantly blocked noninflammatory cell apoptosis induced byA. actinomycetemcomitansinfection in gingival epithelium and connective tissue (P< 0.05). These results provide new insight into how diabetes aggravatesA. actinomycetemcomitans-induced periodontal destruction in rats by significantly increasing the inflammatory response, leading to increased bone loss and enhancing apoptosis of gingival epithelial and connective tissue cells through a caspase-3-dependent mechanism. Antibiotics had a more pronounced effect on many of these parameters in diabetic than in normoglycemic rats, suggesting a deficiency in the capacity of diabetic animals to resist infection.

Radiation-induced gastric epithelial apoptosis occurs in the proliferative zone and is regulated by p53, bak, bax, and bcl-2

2007 ◽  
Vol 292 (2) ◽  
pp. G620-G627 ◽  
Author(s):  
S. M. C. Przemeck ◽  
C. A. Duckworth ◽  
D. M. Pritchard
Keyword(s):  
Small Intestine ◽  
Epithelial Cells ◽  
Gastric Epithelium ◽  
Apoptotic Cells ◽  
Gastric Epithelial Cells ◽  
Wild Type ◽  
Γ Radiation ◽  
Proliferative Zone ◽  
Radiation Induced ◽  
Hematoxylin And Eosin

Unlike the small intestine and colon where γ-radiation-induced apoptosis has previously been well characterized, the response of murine gastric epithelium to γ-radiation has not been investigated in detail. Apoptosis was therefore assessed on a cell positional basis in gastric antral and corpus glands from adult male mice following γ-radiation. Maximum numbers of apoptotic cells were observed in both antrum and corpus at 48 h and at radiation doses greater than 12 Gy. However, the number of apoptotic cells observed in the gastric epithelium was much lower than observed in the small intestine or colon after similar doses of radiation. Hematoxylin and eosin, caspase 3 immunohistochemistry, and terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick-end labeling detected similar numbers and cell positional distributions of apoptotic cells, hence hematoxylin and eosin was used for subsequent studies. The highest numbers of apoptotic cells were observed at cell positions 5–6 in the antrum and cell positions 15–18 in the corpus. These distributions coincided with the distributions of PCNA-labeled proliferating cells, but not with the distributions of H+-K+-ATPase-labeled parietal cells or TFF2-labeled mucous neck cells. Decreased numbers of apoptotic gastric epithelial cells were observed in p53-null, bak-null, and bax-null mice compared with wild-type counterparts 6 and 48 h after 12 Gy γ-radiation. Significantly increased numbers of apoptotic gastric epithelial cells were observed in bcl-2-null mice compared with wild-type littermates 6 h after 12 Gy γ-radiation. Radiation therefore induces apoptosis in the proliferative zone of mouse gastric epithelium. This response is regulated by the expression of p53, bak, bax, and bcl-2.

scholarly journals Pathomorphology of cats with myocardial infectious peritonitis

2019 ◽  
Vol 21 (96) ◽  
pp. 177-184
Author(s):  
G. I. Kotsyumbas ◽  
M. R. Khalaniia
Keyword(s):  
Connective Tissue ◽  
Contractile Function ◽  
Rapid Test ◽  
Methyl Green ◽  
Structural Elements ◽  
Neutral Solution ◽  
Pas Reaction ◽  
Inflammatory Processes ◽  
Hematoxylin And Eosin ◽  
Hematoxylin And Eosin Staining

The article presents the results of macroscopic and microscopic examinations of myocardial cats in wet and mixed forms of FIP. A pathoanatomical study of 19 cat carcasses, aged from 3 months to 7 years, was diagnosed with infectious peritonitis during life (on the basis of anamnesis, clinical features, morphological and biochemical blood test, ultrasound, Rivalt test and FCVetx rapid test VetE.) All animals were kept at home. For the microstructural study, samples of cats' hearts were selected, which were fixed in 10% aqueous formalin neutral solution, Carnua, Buen solutions and 96 ° ethyl alcohol. Histogram sections were stained with hematoxylin and eosin staining, picrofuxin (Van Gizon), PAS reaction (McManus), methyl green pyronin (Brache), Malory, and examined under a microscope. Histological examination of the cardiac muscle of cats in various forms of infectious peritonitis revealed changes of non-inflammatory and inflammatory nature. In the exudative form, non-inflammatory processes prevailed. In the myocardium, the most severe changes occurred in the capillaries, the walls of the arterial vessels and the stroma, which were characterized by diapedic hemorrhage, mucoid and fibrinoid swelling and necrosis of the walls of the arterioles. Disorganization of connective tissue was accompanied by stratification of connective tissue fibers and impregnation of weakly oxyphilic, PAS-positive compounds of the intermuscular lumen, which was combined with dystrophic changes in cardiomyocytes. In the mixed form, proliferative-destructive vasculitis, diffuse or focal lymphoid-histiocytic infiltrates in the myocardium prevail. The revealed optical changes in the structural elements of the heart indicated a sharp weakening of the contractile function of cardiomyocytes and heart failure. In addition, it should be noted that the characteristic morphological manifestation for immunocomplex diseases is the development of vasculitis, which is preceded by fibrinoid necrosis of the walls of the arterial vessels and intensive infiltration of their circulatory elements, and these changes occurred in infectious peritonitis of cats.

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