scholarly journals Comparison of disinfectants by immersion and spray atomization techniques on the linear dimensional stability of different interocclusal recording materials: An in vitro study

2016 ◽  
Vol 10 (01) ◽  
pp. 007-015 ◽  
Author(s):  
Revathy Gounder ◽  
B. V. J. Vikas
Keyword(s):  
Sodium Hypochlorite ◽  
Dimensional Stability ◽  
Dimensional Change ◽  
Chlorhexidine Gluconate ◽  
Spray Atomization ◽  
Time Duration ◽  
Significance Level ◽  
Significant Difference ◽  
Immersion Technique

ABSTRACT Objective: To evaluate and compare the effect of 0.5% chlorhexidine gluconate, 1% sodium hypochlorite, and 2% glutaraldehyde by immersion and spray atomization technique on the linear dimensional stability of Jet bite, Aluwax and Ramitec interocclusal recording materials. Materials and Methods: Three representative materials: Jet bite (addition silicone), Aluwax and Ramitec (polyether) were mixed according to manufacturer's instructions and then specimens were prepared according to the specifications of ISO 4823. All the specimens except the control (distilled water) were treated with disinfectant solutions (0.5% chlorhexidine gluconate, 1% sodium hypochlorite, and 2% glutaraldehyde) for 30 and 60 min (n = 10) by spray and immersion technique. Once removed from the solutions, the test samples were washed in water for 15 s, dried and measured after 24 h 3 times using a measuring microscope with an accuracy of 0.0001 mm. Two-way ANOVA and Tukey's test with significance level of 5% were used to assess the statistical data (α = 0.05). Result: All groups showed no significant difference statistically, in linear dimension when disinfected for 30 min by spray or immersion technique. Polyether had significantly higher dimensional variation when immersed in sodium hypochlorite for 60 min. Addition silicone showed the least dimensional change which ranged from 0.024% to 0.05%, followed by polyether from 0.004% to 0.171% and Aluwax from 0.146% to 0.228%. Conclusion: To preserve the dimensions and surface of the recording materials and effective microbial elimination, restrictions should be applied in the method of disinfection and time duration. However, using the disinfectants either by spray or immersion technique, the dimensional change was <0.5% which was not clinically significant according to the American Dental Association specification no. 19 criteria within the first 24 h.

scholarly journals Antimicrobial Efficacy of Octenidine Hydrochloride, Green Tea, Sodium Hypochlorite and Chlorhexidine Gluconate as Retreatment Endodontic Irrigant Against E. faecalis, Candida & Mixed Culture – In-Vitro Study

2021 ◽  
Vol 10 (32) ◽  
pp. 2629-2633
Author(s):  
Reetika S. Khandelwal ◽  
Shivkumar P. Mantri ◽  
Bonny Paul ◽  
Kavita A. Dube ◽  
Gargi Mishra ◽  
...  
Keyword(s):  
Candida Albicans ◽  
Mixed Culture ◽  
Green Tea ◽  
Sodium Hypochlorite ◽  
Culture Broth ◽  
Chlorhexidine Gluconate ◽  
Antimicrobial Efficacy ◽  
Significant Difference ◽  
Better Than

BACKGROUND Sodium hypochlorite & chlorhexidine are usually employed as endodontic irrigants due to their antimicrobial properties, however, their cytotoxicity & allergenicity issues demand an equally effective but safer irrigant. The purpose of the study was to compare the antimicrobial efficacy of octenidine hydrochloride (OCT), green tea, sodium hypochlorite and chlorhexidine (CHX) at various concentrations against E. faecalis, C. albicans and its mixture. METHODS The study was conducted in Hitkarini Dental College, Jabalpur. Various concentrations of irrigants were prepared. Muller Hinton agar media plates were swabbed with the culture broth of E faecalis ATCC 29212, Candida albicans ATCC 10231 or a mixture of these. Five Whatman paper disks (6 mm), each loaded with experimental concentration of the irrigant, were placed at equal distance on to the plate. The plates were incubated at 370 C for 48 hours. This was done in 6 replicates. The plates were evaluated for clear zone of inhibition measured in mm. RESULTS The diameter readings were analysed using Kruskal Wallis and Mann Whitney U tests. There was a significant difference between the groups against Enterococcus faecalis; candida, & mixed culture (P < 0.01). CONCLUSIONS 5 % Sodium hypochlorite is better than all others whereas 2 % CHX is better than OCT & Green tea. 0.1 % OCT is better than 3 % Green tea. OCT can be used as a contributory irrigant along with NaOCl to achieve antimicrobial advantage. KEY WORDS Antimicrobial Efficacy, Candida Albicans, Chlorhexidine Gluconate, E Faecalis, Green Tea, Octenidine Hydrochloride, Sodium Hypochlorite

scholarly journals Comparison of cytotoxicity of various concentrations origanum extract solution with 2% chlorhexidine gluconate and 5.25% sodium hypochlorite

2015 ◽  
Vol 09 (01) ◽  
pp. 006-010 ◽  
Author(s):  
Evren Ok ◽  
Necdet Adanir ◽  
Sema Hakki
Keyword(s):  
Sodium Hypochlorite ◽  
Cytotoxic Effect ◽  
Statistical Significance ◽  
In Vitro Study ◽  
Solution Concentration ◽  
Chlorhexidine Gluconate ◽  
Extract Solution ◽  
Root Canal Irrigation ◽  
Significant Difference

ABSTRACT Objective: The purpose of this in vitro study was to compare the cytotoxicity of 0.5–4.5 origanum extract solution (OES), 2% chlorhexidine gluconate (CHX) and 5.25% sodium hypochlorite (NaOCl) with WST-1 test on human periodontal ligament (hPDL) fibroblasts. Materials and Methods: About 0.5–4.5% OES, 2% CHX and 5.25% NaOCl solutions cytotoxicity was evaluated with cell culture test using PDL fibroblasts. Viability of hPDL cells was evaluated with WST-1 (Cell Proliferation Reagent WST-1 Roche) test at 1, 24 and 72nd h. hPDL cells were plated at 20 × 103 cells per well in 96-well plates. Absorbance values were read in optical density 480 nm by ELISA plate reader spectrophotometer. The statistical differences between various groups were evaluated using one-way ANOVA, post-hoc Duncan's Multiple Range test using SAS software. Statistically, a significant difference was considered at P < 0.001. Results: According to the 1-h cytotoxicity results, 0.5% OES showed the least cytotoxic effect in test groups. There were not found any statistical significance between 1% OES and 2% CHX. About 5.25% NaOCl showed more cytotoxic effect than 1% OES and 2% CHX. In 24 and 72 h, different concentrations of OES, 5.25% NaOCl, 2% CHX solutions showed similar cytotoxic effect. Conclusions: Based on these results, 1% OES and 2% CHX showed similar results and less cytotoxic effect than 5.25% NaOCl. It could be considered as a favorable solution concentration when OES was used as root canal irrigation solution.

scholarly journals An in vitro comparison of Antimicrobial Efficacy of Three Root Canal Irrigants—BioPure MTAD, 2% Chlorhexidine Gluconate and 5.25% Sodium Hypochlorite as a Final Rinse against E. faecalis

2013 ◽  
Vol 14 (5) ◽  
pp. 842-847 ◽  
Author(s):  
MS Rama Rao ◽  
Vinod Agrawal ◽  
Kanupriya Dhingra ◽  
V Rajesh Gopal ◽  
Abhijita Mohapatra
Keyword(s):  
Sodium Hypochlorite ◽  
Root Canal ◽  
Antibacterial Activities ◽  
Chlorhexidine Gluconate ◽  
Antimicrobial Efficacy ◽  
Pulp Tissue ◽  
Root Canal Irrigants ◽  
Significant Difference ◽  
In Vitro Comparison

ABSTRACT Aim This study was conducted to evaluate the antimicrobial activity of 5.25% sodium hypochlorite (NaOCl), 2% chlorhexidine (CHX) and BioPure MTAD when used as a final rinse against Enterococcus faecalis. Materials and methods Sixty single-rooted premolars were biomechanically prepared, inoculated with E. faecalis and divided into various groups. These were then irrigated with the test irrigants and tested microbiologically for growth of E. faecalis immediately after irrigation and after 48 hours. Results Statistical analysis showed that there was a significant difference between the antibacterial activities of BioPure MTAD, 2% CHX and 5.25% NaOCl at 5 minutes; however, the antibacterial activities of the three irrigants were comparable after 2 days of irrigation Conclusion The present study concludes that BioPure MTAD is as effective against E. faecalis as 5.25% NaOCl and more effective than 2% CHX. Clinical significance E. faecalis is one of the most resistant intracanal species and a possible cause of root canal failure. Many authors have stressed the importance of using antimicrobial irrigants during chemomechanical preparation to ensure complete disinfection. Therefore, various irrigating solutions have been used during and immediately after root canal preparation to remove debris and necrotic pulp tissue and to eliminate microorganisms that cannot be reached by mechanical instrumentation. How to cite this article Agrawal V, Rao MSR, Dhingra K, Gopal VR, Mohapatra A, Mohapatra A. An in vitro comparison of Antimicrobial Efficacy of Three Root Canal Irrigants—BioPure MTAD, 2% Chlorhexidine Gluconate and 5.25% Sodium Hypochlorite as a Final Rinse against E. faecalis. J Contemp Dent Pract 2013;14(5):842-847.

Comparative Study to Evaluate the Surface Detail Reproduction of Dental Stone after Immersion in Various Different Disinfectant Solutions, Under Stereomicroscope 10 X Magnification –An In-Vitro Study

2017 ◽  
Vol 9 (3) ◽  
Author(s):  
Rathika Rai ◽  
M. A. Easwaran ◽  
K. T. Dhivya
Keyword(s):  
Sodium Hypochlorite ◽  
In Vitro Study ◽  
Control Group ◽  
Immersion Method ◽  
Significant Difference ◽  
Surface Irregularities ◽  
Disinfectant Solution ◽  
Surface Detail ◽  
Group B

Aim: To evaluate the surface detail reproduction of dental stone this is immersed in different disinfectant solution and studied under stereomicroscope. Methodology: Total number of 30 specimens of dental stone (Type III) were made with measurements of 1.5cm diameter and 1cm height .This samples are divided in to 3 groups group A,B,C. were A is immersed in Distilled water which was taken as control group ;B is immersed in 2% Glutaraldehyde and C is immersed in 5%sodium hypochlorite. Each specimen were immersed in the disinfectant solution for 15 minutes and dried under room temperature for 24 hrs. After 24 hrs each specimens are studied under stereomicroscope for surface details. Result: The results showed no significant difference in the surface irregularities and porosities for a group 1 and group 2 except group 3 which showed significant increase in the porosities, surface irregularities and erosions after disinfection with 5% NaHOCl by immersion method. Conclusion: The surface detail reproduction capacity of die stone was adversely affected when 5% Sodium hypochlorite was used as disinfectant solution when compare d to control group and 2% Glutaraldehyde

Materials Sealing Preventing Biofilm Formation in Implant/Abutment Joints: Which Is the Most Effective? A Systematic Review and Meta-Analysis

2020 ◽  
Vol 46 (2) ◽  
pp. 163-171
Author(s):  
Cecília Alves de Sousa ◽  
Maria Beatriz Bello Taborda ◽  
Gustavo Antônio Correa Momesso ◽  
Eduardo Passos Rocha ◽  
Paulo Henrique dos Santos ◽  
...  
Keyword(s):  
Systematic Review ◽  
Biofilm Formation ◽  
Meta Analysis ◽  
Significance Level ◽  
Gutta Percha ◽  
Significant Difference ◽  
Physical Barriers ◽  
External Connection

The purpose of this systematic review was to evaluate the literature available for materials exhibiting the best efficacy in preventing biofilm formation in the interior of implants. We searched PubMed/MEDLINE, Scopus, and Cochrane databases. This review is registered with the PROSPERO database and followed the suitability of the PRISMA protocol. The initial search resulted in 326 articles from the databases. After they were read, 8 articles remained, and the inclusion and exclusion criteria were applied. Six of these 8 articles were classified as in vitro and 2 were classified as in situ. The regions of the implants evaluated ranged from the interface of the pieces to the occlusal upper access of the abutment. The implant connections evaluated the Morse taper, external connection, and internal connection. Meta-analysis of the quantitative data was performed at a significance level of .05. Cotton exhibited poor control of infiltration, even in combination with other materials. Isolated gutta-percha (GP) and polytetrafluoroethylene (PTFE) tape with composite resin (CR) or GP performed better as physical barriers. The best results for chemical barriers were observed by the application of 1% chlorhexidine gluconate (CG) gel, thymol varnish, and the deposition of Ag films onto the surface. The applied meta-analysis did not show a significant difference in comparison between the different types of implant connections (P &gt; .05). The application of CG and thymol varnish antimicrobials was effective in preventing biofilm formation and easy clinical execution; these could be used in combination with CR, GP, and PTFE.

Effects of Load Cycling on the Microleakage of Beveled and Nonbeveled Margins in Class V Resin-Based Composite Restorations

2010 ◽  
Vol 11 (5) ◽  
pp. 25-32 ◽  
Author(s):  
Hamideh Ameri ◽  
Marjaneh Ghavamnasiri ◽  
Ehsan Abdoli
Keyword(s):  
High Speed ◽  
Class V ◽  
Significance Level ◽  
Gingival Margin ◽  
Composite Restorations ◽  
Nail Polish ◽  
Significant Difference ◽  
Load Cycling ◽  
Composite Restoration

Abstract Aim This study evaluated the influence of mechanical loading and thermocycling on microleakage of class V resin-based composite restorations with and without enamel bevel. Methods and Materials Sixty class V cavity preparations measuring 3.0 mm wide (mesiogingivally) x 2.0 mm high (occluso-gingivally) x 1.5 mm deep with the occlusal margin in enamel and the gingival margin in cementum were prepared on the buccal surfaces of human premolars using a #12 diamond round bur (Drendel & Zweiling Diamant GmbH, Lemgo, Germany) in a high-speed, water-cooled handpiece. The specimens were then divided into two groups of 30 specimens each, based on the type of enamel cavosurface margin configuration as beveled or nonbeveled (butt joint). After restoring the preparations with a flowable resin-based composite (Tetric Flow, Ivoclar Vivadent-AG, Schaan, Liechtenstein) and finishing and polishing with sequential discs (Sof-Lex Pop-on, 3M-ESPE, St. Paul, MN, USA), the teeth were stored at 37°C and 100 percent humidity. Twenty-four hours later, half of the specimens in each group (nonbeveled “N” or beveled “B”) were exposed to a cycling loading for 250,000 cycles to simulate occlusal loading and assigned to two subgroups (NL+ or BL+), while the remainder of the specimens in each group were only maintained in a 100-percent-humidity environment, without any cyclical loading, until tested (NL– or BL–). The specimens were sealed with sticky wax (Kemdent, Associated Dental Products, Swindon, UK) and nail polish. The apical foramen of each tooth was sealed with sticky wax and the rest of the tooth was covered with nail varnish, except for an area within 1.0 mm around the composite restoration. To detect marginal leakage, all of the samples were stored in a 0.5 percent basic fuchsine solution for 24 hours. The specimens were then sectioned longitudinally using a low-speed diamond blade (IsoMet, Buehler Ltd., Lake Bluff, IL, USA), machined, and evaluated under 25X magnification using a stereomicroscope (M9, Wild Heerbrugg, Switzerland). The specimens were scored on a scale from 1 to 4 on the degree of dye penetration. The qualitative data were analyzed by the Mann- Whitney U test at a 5 percent significance level (p<0.05). The null hypothesis of this study was that there is no difference in microleakage between beveled and nonbeveled class V buccal preparations in premolar teeth restored with resinbased composite and subjected to simulated occlusal loading and thermocycling. Results In each group the gingival margin showed significantly more microleakage than the enamel margin (p<0.05). Load cycling did not result in an increase in microleakage in nonbeveled (p=0.259) or in beveled (p=0.053) occlusal margins. However, the gingival margins showed a statistically significant difference in microleakage after load cycling whether in cavities with enamel occlusal bevel (p=0.004) or in groups without a bevel. This means the enamel margin configuration of the enamel occlusal margin had no effect on decreasing microleakage in the gingival aspect of class V composite restorations. In general, the nonbeveled preparations in this study had significantly less microleakage than the bevel specimens whether they were loaded occlusally or not (p=0.001). Clinical Significance Within the limitations of this in vitro study, no benefit was derived from placing an enamel cavosurface bevel on the occlusal margin of a standardized class V composite restoration located at the cementoenamel junction. The most important consideration is to prevent microleakage along the gingival margin regardless of whether the occlusal enamel margin is beveled. Citation Ameri H, Ghavamnasiri M, Abdoli E. Effects of load cycling on the microleakage of beveled and nonbeveled occlusal margins in class V resin-based composite restorations. J Contemp Dent Pract [Internet]. 2010 October; 11(5):025- 032. Available from: http://www.thejcdp.com/ journal/view/volume11-issue5-ghavamnasiri

A phase II, randomized, double blind comparison of calcium aluminosilicate clay (CASAD) versus placebo (dibasic calcium carbonate) for the prevention of diarrhea in patients (pts) with metastatic colorectal cancer (mCRC) treated with irinotecan (I).

2013 ◽  
Vol 31 (15_suppl) ◽  
pp. 3600-3600
Author(s):  
Bryan K. Kee ◽  
Rebecca Slack ◽  
Todd S. Crocenzi ◽  
Lucas Wong ◽  
Benjamin Esparaz ◽  
...  
Keyword(s):  
Phase Ii ◽  
Performance Status ◽  
Symptom Burden ◽  
Active Metabolites ◽  
Serious Adverse Events ◽  
Double Blind ◽  
Significance Level ◽  
Significant Difference ◽  
Grade 3

3600 Background: CASAD is a naturally occurring calcium montmorrilonite clay that serves as a cation exchange absorbent. One of the active metabolites of Irinotecan is SN-38, which is adsorbed by CASAD in vitro. The study hypothesis was that oral CASAD would reduce the rate of grade 3/4 diarrhea in mCRC patients treated with irinotecan. Methods: The study is a multicenter, prospective, randomized, double blinded placebo-controlled phase II trial. One hundred patients receiving I-based chemotherapy were randomized equally between CASAD (1000 mg po 4x daily) and placebo in order to have 75% power to detect a difference in the proportions of patients with grade 3/4 diarrhea within 6 weeks at a 1-sided 5% significance level. We also compared symptom burden using the MDASI questionnaire summed over the 13 symptom items for weeks 0, 3, 5, and 6. Results: Between 5/2009 and 5/2012, 100 patients were randomized in a 1:1 ratio between study arms. Median age 57 yrs, 54% male, 74% Non-Hispanic White, 93% performance status 0 or 1. Serious diarrhea was less frequent than expected based upon prior studies with Irinotecan. In evaluable patients, no significant difference in the rate of G3/4 diarrhea was seen (the primary endpoint): CASAD arm: 7/43 pts (16%), Placebo arm: 3/32 pts (9%), p=0.70. The rate of any diarrhea among all pts was also similar: CASAD arm 64% vs. Placebo arm 70%. The rate of study dropout was 14% in CASAD and 38% for placebo (p=0.01; 2-sided). No differences were found in symptom burden or individual symptom items or serious adverse events. Conclusions: Compared with placebo, CASAD use was safe but ineffective in preventing diarrhea in mCRC patients treated with irinotecan-containing chemotherapy regimens. There were no favorable or unfavorable signals in terms of the patient experience related to symptoms, but there were significantly more dropouts in the placebo arm. Future CASAD trials are focused on active treatment of diarrhea. Clinical trial information: NCT00748215.

Marginal Gap Formation in Approximal “Bulk Fill” Resin Composite Restorations After Artificial Ageing

2018 ◽  
Vol 43 (2) ◽  
pp. 180-189 ◽  
Author(s):  
A Peutzfeldt ◽  
S Mühlebach ◽  
A Lussi ◽  
S Flury
Keyword(s):  
Tap Water ◽  
Resin Composite ◽  
Class Ii ◽  
Artificial Ageing ◽  
Resin Composites ◽  
Gap Formation ◽  
Marginal Gap ◽  
Significance Level ◽  
Significant Difference

SUMMARY The aim of this in vitro study was to investigate the marginal gap formation of a packable “regular” resin composite (Filtek Supreme XTE [3M ESPE]) and two flowable “bulk fill” resin composites (Filtek Bulk Fill [3M ESPE] and SDR [DENTSPLY DeTrey]) along the approximal margins of Class II restorations. In each of 39 extracted human molars (n=13 per resin composite), mesial and distal Class II cavities were prepared, placing the gingival margins below the cemento-enamel junction. The cavities were restored with the adhesive system OptiBond FL (Kerr) and one of the three resin composites. After restoration, each molar was cut in half in the oro-vestibular direction between the two restorations, resulting in two specimens per molar. Polyvinylsiloxane impressions were taken and “baseline” replicas were produced. The specimens were then divided into two groups: At the beginning of each month over the course of six months' tap water storage (37°C), one specimen per molar was subjected to mechanical toothbrushing, whereas the other was subjected to thermocycling. After artificial ageing, “final” replicas were produced. Baseline and final replicas were examined under the scanning electron microscope (SEM), and the SEM micrographs were used to determine the percentage of marginal gap formation in enamel or dentin. Paramarginal gaps were registered. The percentages of marginal gap formation were statistically analyzed with a nonparametric analysis of variance followed by Wilcoxon-Mann-Whitney tests and Wilcoxon signed rank tests, and all p-values were corrected with the Bonferroni-Holm adjustment for multiple testing (significance level: α=0.05). Paramarginal gaps were analyzed descriptively. In enamel, significantly lower marginal gap formation was found for Filtek Supreme XTE compared to Filtek Bulk Fill (p=0.0052) and SDR (p=0.0289), with no significant difference between Filtek Bulk Fill and SDR (p=0.4072). In dentin, significantly lower marginal gap formation was found for SDR compared to Filtek Supreme XTE (p&lt;0.0001) and Filtek Bulk Fill (p=0.0015), with no significant difference between Filtek Supreme XTE and Filtek Bulk Fill (p=0.4919). Marginal gap formation in dentin was significantly lower than in enamel (p&lt;0.0001). The percentage of restorations with paramarginal gaps varied between 0% and 85%, and for all three resin composites the percentages were markedly higher after artificial ageing. The results from this study suggest that in terms of marginal gap formation in enamel, packable resin composites may be superior to flowable “bulk fill” resin composites, while in dentin some flowable “bulk fill” resin composites may be superior to packable ones.

scholarly journals Extended-pour and conventional alginates: effect of storage time on dimensional accuracy and maintenance of details

2021 ◽  
Vol 26 (3) ◽  
Author(s):  
Sandro Basso BITENCOURT ◽  
Isabela Araguê CATANOZE ◽  
Emily Vivianne Freitas da SILVA ◽  
Karina Helga Leal TURCIO ◽  
Daniela Micheline dos SANTOS ◽  
...  
Keyword(s):  
Dimensional Stability ◽  
High Stability ◽  
Digital Camera ◽  
Dimensional Accuracy ◽  
Dimensional Change ◽  
Clinical Results ◽  
Dimensional Changes ◽  
Plastic Bags ◽  
Significant Difference ◽  
Student’S T

ABSTRACT Objective: This study aimed to evaluate the dimensional stability and maintenance of details of conventional and high stability alginates up to 5-day storage. Methods: Two types of alginates were selected (n=10) for this study, conventional (Hydrogum) and high stability alginates (Hydrogum 5), which were produced with the aid of a cylindrical metal block and a ring-shaped metal mold (Specifications 18, 19, and 25, ANSI/ADA). Ten images were obtained from the molds for the dimensional stability test, which were taken immediately after their production and at each different storage periods (15 min, 24 h, 48 h, 72 h, 96 h, and 120 h) by a digital camera. The specimens were kept hermetically sealed in plastic bags (23°C) and then used to obtain 140 (n=70) dental stone models, used in the detail reproduction test, in which the angular accuracy of three grooves (20 µm, 50 µm, and 75 µm) was observed at each period. The details reproduction accuracy was classified using a predetermined score classification. Measurements of dimensional changes were made in the Corel DRAW X6 program. The data were submitted to the Student’s t-test (α?#8197;= 0.05). Results: A statistically significant difference concerning the size of the matrix was observed after 24h for both alginates, and a statistically significant negative linear dimensional change (contraction) was verified after 24 h of storage (1.52% for the high stability alginate, and 1.32% for the conventional alginate). The high stability alginate kept the full details for 72 hours, while the conventional alginate, for 24 h. Both alginates reproduced the 75 µm groove at all storage periods. Conclusion: Impressions made with both alginates presented satisfactory clinical results when the alginates were immediately poured.

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