scholarly journals Comparison of cytotoxicity of various concentrations origanum extract solution with 2% chlorhexidine gluconate and 5.25% sodium hypochlorite

2015 ◽  
Vol 09 (01) ◽  
pp. 006-010 ◽  
Author(s):  
Evren Ok ◽  
Necdet Adanir ◽  
Sema Hakki
Keyword(s):  
Sodium Hypochlorite ◽  
Cytotoxic Effect ◽  
Statistical Significance ◽  
In Vitro Study ◽  
Solution Concentration ◽  
Chlorhexidine Gluconate ◽  
Extract Solution ◽  
Root Canal Irrigation ◽  
Significant Difference

ABSTRACT Objective: The purpose of this in vitro study was to compare the cytotoxicity of 0.5–4.5 origanum extract solution (OES), 2% chlorhexidine gluconate (CHX) and 5.25% sodium hypochlorite (NaOCl) with WST-1 test on human periodontal ligament (hPDL) fibroblasts. Materials and Methods: About 0.5–4.5% OES, 2% CHX and 5.25% NaOCl solutions cytotoxicity was evaluated with cell culture test using PDL fibroblasts. Viability of hPDL cells was evaluated with WST-1 (Cell Proliferation Reagent WST-1 Roche) test at 1, 24 and 72nd h. hPDL cells were plated at 20 × 103 cells per well in 96-well plates. Absorbance values were read in optical density 480 nm by ELISA plate reader spectrophotometer. The statistical differences between various groups were evaluated using one-way ANOVA, post-hoc Duncan's Multiple Range test using SAS software. Statistically, a significant difference was considered at P < 0.001. Results: According to the 1-h cytotoxicity results, 0.5% OES showed the least cytotoxic effect in test groups. There were not found any statistical significance between 1% OES and 2% CHX. About 5.25% NaOCl showed more cytotoxic effect than 1% OES and 2% CHX. In 24 and 72 h, different concentrations of OES, 5.25% NaOCl, 2% CHX solutions showed similar cytotoxic effect. Conclusions: Based on these results, 1% OES and 2% CHX showed similar results and less cytotoxic effect than 5.25% NaOCl. It could be considered as a favorable solution concentration when OES was used as root canal irrigation solution.

Comparative Study to Evaluate the Surface Detail Reproduction of Dental Stone after Immersion in Various Different Disinfectant Solutions, Under Stereomicroscope 10 X Magnification –An In-Vitro Study

2017 ◽  
Vol 9 (3) ◽  
Author(s):  
Rathika Rai ◽  
M. A. Easwaran ◽  
K. T. Dhivya
Keyword(s):  
Sodium Hypochlorite ◽  
In Vitro Study ◽  
Control Group ◽  
Immersion Method ◽  
Significant Difference ◽  
Surface Irregularities ◽  
Disinfectant Solution ◽  
Surface Detail ◽  
Group B

Aim: To evaluate the surface detail reproduction of dental stone this is immersed in different disinfectant solution and studied under stereomicroscope. Methodology: Total number of 30 specimens of dental stone (Type III) were made with measurements of 1.5cm diameter and 1cm height .This samples are divided in to 3 groups group A,B,C. were A is immersed in Distilled water which was taken as control group ;B is immersed in 2% Glutaraldehyde and C is immersed in 5%sodium hypochlorite. Each specimen were immersed in the disinfectant solution for 15 minutes and dried under room temperature for 24 hrs. After 24 hrs each specimens are studied under stereomicroscope for surface details. Result: The results showed no significant difference in the surface irregularities and porosities for a group 1 and group 2 except group 3 which showed significant increase in the porosities, surface irregularities and erosions after disinfection with 5% NaHOCl by immersion method. Conclusion: The surface detail reproduction capacity of die stone was adversely affected when 5% Sodium hypochlorite was used as disinfectant solution when compare d to control group and 2% Glutaraldehyde

scholarly journals A Comparative in Vitro Study of Power Output Deterioration over Time Between Ho:YAG Laser Fibers from Different Manufacturers as a Function of Deflection and Power Input

2015 ◽  
Vol 9 (1) ◽  
pp. 12-18
Author(s):  
Andreas Bourdoumis ◽  
Panagiotis Christopoulos ◽  
Nirmal Raj ◽  
Artemis Fedder ◽  
Noor Buchholz
Keyword(s):  
Power Output ◽  
Fiber Type ◽  
Statistical Significance ◽  
In Vitro Study ◽  
Power Input ◽  
Energy Output ◽  
Fiber Fracture ◽  
Significant Difference ◽  
Continuous Use

Objectives: To investigate the performance of laser fibers from 6 major manufacturers in vitro and to identify the effect of time and angulations (180° and 0°) on fiber power output. Materials and Methods: Overall, 36 single-use fibers were used. Each was tested with an energy input of 0.8, 1.4 and 2.0 Joules. A power detector measured power output after 1, 5, 10 and 15 minutes for three 15-minute cycles of continuous use. For the first 2 cycles, the fiber was bent to 180° with the use of a pre fabricated mould. Analysis of the data was performed by ANOVA and Tukey's test when the results were significant amongst groups. Statistical significance was deemed p < 0.05. Results: No fiber fracture occurred. There was no significant difference in output at 15 minutes of continuous use at 0° and 180°. The reduction in energy output at the 15th minute of continuous use at 180° was not significant for any fiber type or initial input. Only output differences between the fibers proved to be significant (p = 0.001). Conclusion: Fiber fracture and decline in performance is not due to deflection and continuous use. Frictional forces that occur between the fiber tip and the stone fragments may be responsible.

scholarly journals Antimicrobial Efficacy of Octenidine Hydrochloride, Green Tea, Sodium Hypochlorite and Chlorhexidine Gluconate as Retreatment Endodontic Irrigant Against E. faecalis, Candida & Mixed Culture – In-Vitro Study

2021 ◽  
Vol 10 (32) ◽  
pp. 2629-2633
Author(s):  
Reetika S. Khandelwal ◽  
Shivkumar P. Mantri ◽  
Bonny Paul ◽  
Kavita A. Dube ◽  
Gargi Mishra ◽  
...  
Keyword(s):  
Candida Albicans ◽  
Mixed Culture ◽  
Green Tea ◽  
Sodium Hypochlorite ◽  
Culture Broth ◽  
Chlorhexidine Gluconate ◽  
Antimicrobial Efficacy ◽  
Significant Difference ◽  
Better Than

BACKGROUND Sodium hypochlorite & chlorhexidine are usually employed as endodontic irrigants due to their antimicrobial properties, however, their cytotoxicity & allergenicity issues demand an equally effective but safer irrigant. The purpose of the study was to compare the antimicrobial efficacy of octenidine hydrochloride (OCT), green tea, sodium hypochlorite and chlorhexidine (CHX) at various concentrations against E. faecalis, C. albicans and its mixture. METHODS The study was conducted in Hitkarini Dental College, Jabalpur. Various concentrations of irrigants were prepared. Muller Hinton agar media plates were swabbed with the culture broth of E faecalis ATCC 29212, Candida albicans ATCC 10231 or a mixture of these. Five Whatman paper disks (6 mm), each loaded with experimental concentration of the irrigant, were placed at equal distance on to the plate. The plates were incubated at 370 C for 48 hours. This was done in 6 replicates. The plates were evaluated for clear zone of inhibition measured in mm. RESULTS The diameter readings were analysed using Kruskal Wallis and Mann Whitney U tests. There was a significant difference between the groups against Enterococcus faecalis; candida, & mixed culture (P < 0.01). CONCLUSIONS 5 % Sodium hypochlorite is better than all others whereas 2 % CHX is better than OCT & Green tea. 0.1 % OCT is better than 3 % Green tea. OCT can be used as a contributory irrigant along with NaOCl to achieve antimicrobial advantage. KEY WORDS Antimicrobial Efficacy, Candida Albicans, Chlorhexidine Gluconate, E Faecalis, Green Tea, Octenidine Hydrochloride, Sodium Hypochlorite

scholarly journals Cytotoxic Effect of Addition of Different Concentrations of Nanohydroxyapatite to Resin Modified and Conventional Glass Ionomer Cements on L929 Murine Fibroblasts

2021 ◽  
Author(s):  
Parisa Golkar ◽  
Ladan Ranjbar Omrani ◽  
Shide Zohourinia ◽  
Elham Ahmadi ◽  
Faeze Asadian
Keyword(s):  
Cytotoxic Effect ◽  
In Vitro Study ◽  
Biological Properties ◽  
The Other ◽  
Glass Ionomer Cements ◽  
Glass Ionomer ◽  
Beneficial Effects ◽  
Significant Difference ◽  
Murine Fibroblasts

Objectives: In this study we assessed the cytotoxic effect of nanohydroxyapatite (NHA) incorporated into resin modified and conventional glass ionomer cements (RMGICs and CGICs) on L929 murine fibroblasts. Materials and Methods: In this in vitro study, 0wt%, 1wt%, 2wt%, 5wt%, 7wt% and 10wt% concentrations of NHA were added to Fuji II LC RMGIC and Fuji IX CGIC powders. Eighteen samples (5×3mm) were fabricated from each type of glass ionomer, in six experimental groups (n=3): CG0, CG1, CG2, CG5, CG7, CG10, RMG0, RMG1, RMG2, RMG5, RMG7, and RMG10. Samples were incubated for 72h. The overlaying solution was removed and added to L929 fibroblasts. The methyl thiazolyl tetrazolium bromide (MTT) assay was performed at 24, 48 and 72h. The wavelength was read by a spectrophotometer. Data were analyzed by ANOVA and Tukey’s test. Results: There was no significant difference in cytotoxicity of the two types of glass ionomers, with and without NHA, except for CG0 and RMG0 groups after 72h. RMG0 group was significantly more cytotoxic than the CG0 group (P<0.05). In CG groups during the first 24h, the cytotoxicity of CG5 and CG7 groups was significantly higher than that of CG1; while, there was no significant difference between the RMG groups. Cytotoxicity significantly decreased in all groups after 24h (P<0.05). Conclusion: Incorporation of NHA into Fuji II LC RMGIC and Fuji IX CGIC did not affect their biocompatibility and therefore its addition to these materials can provide favorable biological properties, especially considering its beneficial effects on the other properties of GICs.

scholarly journals INFLUÊNCIA DA MODIFICAÇÃO DA BASE DE COLAGEM E DA CONTAMINAÇÃO SALIVAR NA RESISTÊNCIA DE UNIÃO DE TUBOS ORTODÔNTICOS COLADOS AO ESMALTE HUMANO

2021 ◽  
Vol 58 (1) ◽  
pp. eUJ3657
Author(s):  
Germano Brandão ◽  
◽  
Liliana Ávila Maltagliati ◽  
Ana Carla Raphaelli Nahás-Scocate ◽  
Murilo Matias ◽  
...  
Keyword(s):  
Bond Strength ◽  
Shear Bond Strength ◽  
Statistical Significance ◽  
Testing Machine ◽  
In Vitro Study ◽  
Metal Mesh ◽  
Human Teeth ◽  
Significance Level ◽  
Significant Difference

The objective of this in vitro study was to assess and compare the shear bond strength of conventional and modified orthodontic tubes bonded to the surface of dry and saliva-contaminated enamel. The sample consisted of 40 human teeth, which were randomly divided into four groups according to attachment base and presence or absence of saliva contamination as follows: Group CB, conventional orthodontic tubes without salivary contamination; Group CB-S, conventional orthodontic tubes with salivary contamination; Groups BM, orthodontic tubes modified by welding a metal mesh to their base without salivary contamination; and Group BM-S, modified orthodontic tubes with salivary contamination. Shear bond strength test was performed in a universal testing machine and analysis of the adhesive remnant index (ARI) by optical microscopy. Two-way ANOVA was used, followed by Tukey’s test at a statistical significance level of 5%. The ARI results were analysed descriptively. There was statistically significant difference between the groups regarding the shear bond strength values, with conventional tubes presenting significantly higher values (P < 0.05). In addition, the presence of salivary contamination interfered negatively with the behaviour of conventional tubes only (P < 0.05). Shear bond strength was not improved by increasing the area of the orthodontic tubes. Moreover, salivary contamination influenced negatively the SBS values, but only when conventional tubes were used.

scholarly journals Apical Extrusion of Intracanal Debris Using Two Engine Driven and Step-Back Instrumentation Techniques: An In-Vitro Study

2008 ◽  
Vol 02 (04) ◽  
pp. 233-239 ◽  
Author(s):  
Alper Kustarci ◽  
Neslihan Akdemir ◽  
Seyda Herguner Siso ◽  
Demet Altunbas
Keyword(s):  
Stainless Steel ◽  
Analysis Of Variance ◽  
Statistical Significance ◽  
In Vitro Study ◽  
Type Stainless Steel ◽  
Rotary Instruments ◽  
Apical Extrusion ◽  
Working Length ◽  
Significant Difference

ABSTRACTObjectives: The purpose of this study was to compare in-vitro the amount of debris extruded apically from extracted teeth, using K3, Protaper rotary instruments and manual step-back technique.Methods: Forty five human single-rooted mandibular premolar teeth were randomly divided into 3 groups. The teeth in 3 groups were instrumented until reaching the working length with K3, Protaper rotary instruments and K-type stainless steel instruments with manual step-back technique, respectively. Debris extruded from the apical foramen was collected into centrifuge tubes and the amount was determined. The data obtained were analyzed using Kruskal-Wallis one-way analysis of variance and Mann-Whitney U tests, with P=.05 as the level for statistical significance.Results: Statistically significant difference was observed between K3, Protaper and step-back groups in terms of debris extrusion (P<.05). Step-back group had the highest mean debris weight, which was significantly different from the K3 and Protaper groups (P<.05). The lowest mean debris weight was related to K3 group, which was significantly different from the Protaper group (P<.05).Conclusions: Based on the results, all instrumentation techniques produced debris extrusion. The engine-driven Ni-Ti systems extruded significantly less apical debris than step-back technique. However, Protaper rotary instruments extruded significantly more debris than K3 rotary instruments. (Eur J Dent 2008;2:233-239)

scholarly journals Pulp tissue dissolution capacity of QMix 2in1 irrigation solution

2015 ◽  
Vol 09 (03) ◽  
pp. 423-427 ◽  
Author(s):  
Dilara Arslan ◽  
Mehmet Burak Guneser ◽  
Alper Kustarci ◽  
Kursat Er ◽  
Seyda Herguner Siso
Keyword(s):  
Saline Solution ◽  
In Vitro Study ◽  
Control Group ◽  
Pulp Tissue ◽  
Tissue Samples ◽  
Canal Irrigation ◽  
Root Canal Irrigation ◽  
Significant Difference ◽  
Group 2

ABSTRACT Objective: The aim of this study was to evaluate the tissue dissolution efficacy of four root canal irrigation solutions (sodium hypochlorite [NaOCl], chlorhexidine gluconate [CHX], Octenidine [OCT], and QMix 2in1) on bovine pulp tissue. Materials and Methods: Fifty bovine pulp tissue samples, each weighing 6.55 mg, were prepared and randomly divided into four experimental groups and one control group (n = 10) according to the dissolution irrigants used: (1) 5.25% NaOCl group; (2) 2% CHX group; (3) OCT group; (4) QMix 2in1 group; and (5) control group (saline solution). These samples were then placed into special bovine dentin reservoir models and immersed for 1 h with each test solution (0.1 mL of each) at room temperature. The pulp samples were then blotted dry and weighed again. The percentage of weight loss was calculated. Statistically analyzed with one-way analysis of variance and post-hoc Tukey tests (P = 0.05). Results: Saline solution did not dissolve the bovine pulp tissue. All groups, except OCT, dissolved pulp samples more effectively than the control group (P < 0.05). The highest tissue dissolution was observed in 5.25% NaOCl group (P < 0.05). No statistically significant difference was found between the tissue-dissolving effect between QMix 2in1 and those of 2% CHX. Conclusions: Within the limitations of this in vitro study, NaOCl exhibited the best tissue-dissolving effect out of all solutions tested. CHX and QMix 2in1 were able to dissolve pulp tissue but less than NaOCl. OCT and saline solutions could not exhibit significantly tissue-dissolving effectiveness. This study shown that QMix 2in1 has little capacity to dissolve pulp tissue therefore used alone is not sufficient for this purpose.

scholarly journals Comparison of Sealing Efficiency of Mineral Trioxide Aggregate and Biodentine used in Perforation Repair in Acidic and Neutral Environments- An In-vitro Study

2021 ◽  
Author(s):  
Güney Mustafa Yüzer ◽  
Sadullah Kaya
Keyword(s):  
Statistical Significance ◽  
Mineral Trioxide Aggregate ◽  
In Vitro Study ◽  
P Value ◽  
Technology Application ◽  
Significant Difference ◽  
Acid Environment ◽  
Materials Used

Introduction: Furcation perforation is one of the complications that occur during endodontic treatment due to anatomical differences or iatrogenic causes. Repairing the perforation with a biocompatible material eliminates the connection with the gingival sulcus and positively affects the prognosis. The sealing ability of the repair materials is very important and is affected by the conditions of the environment in which they are applied. Aim: To compare the sealing efficiency of Mineral Trioxide Aggregate (MTA) (Angelus, Londrina, PR, Brazil) and Biodentine (Septodont, Saint Maur des Fosses, France) materials used in the repair of furcation perforations in acidic and neutral environments. Materials and Methods: This in-vitro experimental study was carried out in the Department of Endodontics at Dicle University Faculty of Dentistry, Diyarbakır, Türkiye and Dicle University Science and Technology Application and Research Center from 10th June 2020 to 22nd July 2020. A total of 96 mandibular molars were used in the study. The specimens were randomly divided into two equal groups (n=48). When the perforations were repaired with MTA and Biodentine materials, each group was divided into four subgroups (n=12). These subgroups were kept in Phosphate Buffered Saline (PBS) and Acetic Acid (AA) solutions for different periods of time from 4 or 34 days, and all groups were immersed in methylene blue solution. The dye penetration level of the divided sections was examined under a stereomicroscope (Leica, Wetzlar, Germany). Microleakage data of all groups were statistically analysed using Kolmogorov Smirnov, Shapiro Wilk’s and Mann-Whitney U Test. Statistical significance was set at 0.05, it was stated that there was a significant difference when p-value <0.05. Results: The short-term (4 days) microleakage level of biodentine material in the AA environment was significantly lower than that of MTA material (p-value=0.005). There was no statistically significant difference between the materials in terms of long-term (34 days) microleakage levels in the AA environment (p-value >0.05). The long-term microleakage level in PBS environment of biodentine material was significantly lower than that of the AA environment (p-value=0.008). Conclusion: Lower microleakage levels were observed in the neutral environment than the acid environment with both MTA and biodentine during both holding periods.

scholarly journals Cytotoxic Effect of Gliotoxin, Hemolysin, Protease and Melanin purified from Aspergillus fumigates on REF Cell Line, in vitro Study

2015 ◽  
Vol 9 (2) ◽  
pp. 4-10
Author(s):  
Batool Omran Theeb Theeb ◽  
Farooq Ibrahem Mohammad Mohammad ◽  
Abdulkareem Jasim Hashim Hashim
Keyword(s):  
Growth Inhibition ◽  
Cell Line ◽  
Cytotoxic Effect ◽  
In Vitro Study ◽  
Toxic Substances ◽  
Aspergillus Fumigates ◽  
Significant Difference ◽  
Embryonic Fibroblast ◽  
Compound Concentration

Aspergillus fumigates especially clinical isolate produces a series of toxic substances and proteinaceous hemolysin, protease and pigment like melanin which appear to act in an additive and synergic way on cells. In this study, gliotoxin, hemolysin, Protease, and Melanin were used in an experimental model to study their Cytotoxic activity by evaluating their effect on REF cell line (Rat embryonic fibroblast ), for exposure time of 24 hrs at three different concentrations of each compounds triplicate of each concentration were used, cytotoxicity of the purified compounds were active against REF cell line under study and a toxic effect was clear with a significant difference at the level of probability (p≤ 0.05) and this effect was contrasted among different concentrations for each purified compound growth inhibition of REF cell line was increase gradually with the increase of compound concentration.

scholarly journals Effectiveness of light-emitting diode exposure on photodynamic therapy against Enterococcus faecalis: in vitro study

2020 ◽  
Vol 53 (2) ◽  
pp. 71
Author(s):  
Nanik Zubaidah ◽  
Agus Subiwahjudi ◽  
Dinda Dewi Artini ◽  
Karina Erda Saninggar
Keyword(s):  
Photodynamic Therapy ◽  
Enterococcus Faecalis ◽  
Root Canal ◽  
Pathogenic Bacteria ◽  
In Vitro Study ◽  
Control Group ◽  
Root Canal Irrigation ◽  
Group I ◽  
Significant Difference

Background: A successful root canal treatment eliminates pathogenic bacteria from infected root canals. The most common bacteria in root canal infections is Enterococcus faecalis (E. faecalis), due to its resistance to medicament and root canal irrigation. A photodynamic therapy (PDT) is a method of root canal disinfection that uses a combination of photosensitisers and light activation to eliminate bacteria in the root canal. The duration of the PDT irradiation results in the production of singlet oxygen and reactive oxygen species (ROS) to eliminate the E. faecalis bacteria. Purpose: To analyse the differences in the duration exposure of photodynamic therapy against the E. faecalis bacteria. Methods: The E. faecalis bacteria culture was divided into seven eppendorf tubes. Group I was a control group, and group II, III, IV, V, VI and VII were treated using PDT consisting of Toluidine Blue O (TBO) photosensitiser and light source irradiation for ten, 20, 30, 40, 50 and 60 seconds, respectively. After incubation, the number of bacteria was calculated by the Quebec Colony Counter and analysed using the Kruskal–Wallis test and the Mann–Whitney test (p <0.05). Results: There was a significant difference between the number of E. faecalis bacteria colonies in each treatment group (p <0.05). Group VI and VII, which had a longer exposure to PDT, showed a smaller amount of E. faecalis bacteria. Conclusion: The longer exposure of PDT results in a smaller amount of E. faecalis bacteria. The light irradiation of 50 seconds is the most effective to eliminate E. faecalis bacteria.

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