Platelet-rich plasma and platelet-rich fibrin in periodontal regeneration: A review

Regeneration is defined as the reproduction or reconstruction of a lost part or injury of the body in such a way that the architecture and function of the lost or injured tissue are completely restored. The goal of regenerative periodontal therapy is to restore the structure and function of the periodontium. The positive effects of Platelet-rich plasma (PRP) are attributed to the angiogenic, mitogenic and proliferative capacities of growth factors such as platelet-derived growth factor, transforming growth factor and vascular endothelial growth factor. Platelet-rich fibrin (PRF) is a second generation platelet concentrate that allows fibrin membranes enriched with platelets and growth factors to be obtained after starting an anticoagulant-free blood collection without any biomechanical artificial modification. The objective of this review is to know the efficacy of platelet-rich plasma and platelet-rich fibrin in the periodontal regeneration of intrabony defects. The clinical implications for this autologous material are promising. Further long term, larger, multicentred randomized controlled clinical trials are required to determine the effects of PRP and PRF on the regeneration of alveolar bone due to periodontal disease.

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Biologic Agents for Periodontal Regeneration and Implant Site Development

BioMed Research International ◽

10.1155/2015/957518 ◽

2015 ◽

Vol 2015 ◽

pp. 1-10 ◽

Cited By ~ 18

Author(s):

Fernando Suárez-López del Amo ◽

Alberto Monje ◽

Miguel Padial-Molina ◽

ZhiHui Tang ◽

Hom-Lay Wang

Keyword(s):

Growth Factor ◽

Human Platelet ◽

Platelet Rich Plasma ◽

Periodontal Regeneration ◽

Biologic Agents ◽

Fibroblast Growth Factor 2 ◽

Enamel Matrix ◽

Platelet Rich Fibrin ◽

Site Development ◽

Main Target

The advancement of molecular mediators or biologic agents has increased tremendously during the last decade in periodontology and dental implantology. Implant site development and reconstruction of the lost periodontium represent main fields in which these molecular mediators have been employed and investigated. Different growth factors trigger different reactions in the tissues of the periodontium at various cellular levels. Proliferation, migration, and differentiation constitute the main target areas of these molecular mediators. It was the purpose of this comprehensive review to describe the origin and rationale, evidence, and the most current understanding of the following biologic agents: Recombinant Human Platelet-Derived Growth Factor-BB (rhPDGF-BB), Enamel Matrix Derivate (EMD), Platelet-Rich Plasma (PRP) and Platelet-Rich Fibrin (PRF), Recombinant Human Fibroblast Growth Factor-2 (rhFGF-2), Bone Morphogenic Proteins (BMPs, BMP-2 and BMP-7), Teriparatide PTH, and Growth Differential Factor-5 (GDF-5).

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Platelet analogues in periodontal regeneration: A narrative review

Journal of Oral Medicine Oral Surgery Oral Pathology and Oral Radiology ◽

10.18231/j.jooo.2021.045 ◽

2022 ◽

Vol 7 (4) ◽

pp. 211-215

Author(s):

Vinayaka Ambujakshi Manjunatha ◽

Ankitha Anil Jadhav ◽

Chaitanya Sree Chalichamala ◽

Annavarapu Sahithi ◽

Harsha Madakaripura Dasegowda

Keyword(s):

Sports Medicine ◽

Platelet Rich Plasma ◽

Periodontal Regeneration ◽

Preparation Technique ◽

Time Transfer ◽

Platelet Rich Fibrin ◽

Technological Characteristics ◽

Wide Range ◽

Implant Dentistry ◽

Dental Applications

Platelet analogues (PA; platelet rich plasma and platelet rich fibrin) are autologous bioactive compounds that have a wide range of medical and dental applications, including periodontal, maxillofacial, Plastic surgery and sports medicine. The aim of these technologies is to collect all the constituents of a patient's blood sample so that they can be used to promote tissue regeneration and improve healing. Since 1954, PA has come a long way. Periodontology and implant dentistry have both benefited from the use of PAs. However, the non-standard preparation technique, processing time, transfer of concentrates, centrifugation temperature, vibration, etc. are all variables that contribute to the various results described in the literature. This study will retrace the evolution of PAs, their preparation procedures, their clinical and technological characteristics and their uses.

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Transplantation of Adipose-derived Cells for Periodontal Regeneration: A Systematic Review

Current Stem Cell Research & Therapy ◽

10.2174/1574888x13666181105144430 ◽

2019 ◽

Vol 14 (6) ◽

pp. 504-518 ◽

Cited By ~ 3

Author(s):

Dilcele Silva Moreira Dziedzic ◽

Bassam Felipe Mogharbel ◽

Priscila Elias Ferreira ◽

Ana Carolina Irioda ◽

Katherine Athayde Teixeira de Carvalho

Keyword(s):

Systematic Review ◽

Animal Models ◽

Platelet Rich Plasma ◽

Periodontal Regeneration ◽

In Vitro Studies ◽

In Vivo Studies ◽

Cell Sources ◽

Study Designs

This systematic review evaluated the transplantation of cells derived from adipose tissue for applications in dentistry. SCOPUS, PUBMED and LILACS databases were searched for in vitro studies and pre-clinical animal model studies using the keywords “ADIPOSE”, “CELLS”, and “PERIODONTAL”, with the Boolean operator “AND”. A total of 160 titles and abstracts were identified, and 29 publications met the inclusion criteria, 14 in vitro and 15 in vivo studies. In vitro studies demonstrated that adipose- derived cells stimulate neovascularization, have osteogenic and odontogenic potential; besides adhesion, proliferation and differentiation on probable cell carriers. Preclinical studies described improvement of bone and periodontal healing with the association of adipose-derived cells and the carrier materials tested: Platelet Rich Plasma, Fibrin, Collagen and Synthetic polymer. There is evidence from the current in vitro and in vivo data indicating that adipose-derived cells may contribute to bone and periodontal regeneration. The small quantity of studies and the large variation on study designs, from animal models, cell sources and defect morphology, did not favor a meta-analysis. Additional studies need to be conducted to investigate the regeneration variability and the mechanisms of cell participation in the processes. An overview of animal models, cell sources, and scaffolds, as well as new perspectives are provided for future bone and periodontal regeneration study designs.

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Use of PRP, PRF and CGF in Periodontal Regeneration and Facial Rejuvenation—A Narrative Review

Biology ◽

10.3390/biology10040317 ◽

2021 ◽

Vol 10 (4) ◽

pp. 317

Author(s):

Eitan Mijiritsky ◽

Haya Drora Assaf ◽

Oren Peleg ◽

Maayan Shacham ◽

Loredana Cerroni ◽

...

Keyword(s):

Growth Factors ◽

Platelet Rich Plasma ◽

Periodontal Regeneration ◽

Clinical Work ◽

Gingival Recession ◽

Vital Role ◽

Facial Rejuvenation ◽

Cochrane Library ◽

The Past ◽

Autologous Platelet

Growth factors (GFs) play a vital role in cell proliferation, migration, differentiation and angiogenesis. Autologous platelet concentrates (APCs) which contain high levels of GFs make them especially suitable for periodontal regeneration and facial rejuvenation. The main generations of APCs presented are platelet-rich plasma (PRP), platelet-rich fibrin (PRF) and concentrated growth factor (CGF) techniques. The purpose of this review is to provide the clinician with an overview of APCs’ evolution over the past decade in order to give reliable and useful information to be used in clinical work. This review summarizes the most interesting and novel articles published between 1997 and 2020. Electronic and manual searches were conducted in the following databases: Pubmed, Scopus, Cochrane Library and Embase. The following keywords were used: growth factors, VEGF, TGF-b1, PRP, PRF, CGF and periodontal regeneration and/or facial rejuvenation. A total of 73 articles were finally included. The review then addresses the uses of the three different techniques in the two disciplines, as well as the advantages and limitations of each technique. Overall, PRP is mainly used in cases of hard and soft tissue procedures, while PRF is used in gingival recession and the treatment of furcation and intrabony defects; CGF is mainly used in bone regeneration.

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Response to “Enhancing Fat Graft Survival With Autologous Growth Factors: Platelet-Rich Fibrin (PRF) vs Platelet-Rich Plasma (PRP)”

Aesthetic Surgery Journal ◽

10.1093/asj/sjaa392 ◽

2021 ◽

Author(s):

Panxi Yu ◽

Xiaonan Yang ◽

Zuoliang Qi

Keyword(s):

Growth Factors ◽

Graft Survival ◽

Platelet Rich Plasma ◽

Fat Graft ◽

Platelet Rich Fibrin

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The clinical implications of platelet-rich fibrin on periodontal regeneration: A systematic review

The Saudi Dental Journal ◽

10.1016/j.sdentj.2020.12.002 ◽

2020 ◽

Author(s):

Marwa Madi ◽

Ahmed M. Elakel

Keyword(s):

Systematic Review ◽

Periodontal Regeneration ◽

Clinical Implications ◽

Platelet Rich Fibrin

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Management of necrotic immature permanent maxillary incisors using a modified technique of revascularisation

BMJ Case Reports ◽

10.1136/bcr-2020-240203 ◽

2021 ◽

Vol 14 (3) ◽

pp. e240203

Author(s):

Arun Mayya ◽

Shruti Bhandary ◽

Advith Kolakemar ◽

Ann Mary George

Keyword(s):

Platelet Rich Plasma ◽

Blood Clot ◽

Treatment Options ◽

Permanent Teeth ◽

Treatment Modalities ◽

Successful Outcome ◽

Modified Technique ◽

Platelet Rich Fibrin ◽

Periapical Lesions ◽

Over Time

The management of necrotic immature permanent teeth has always been a challenge to endodontists. Various treatment modalities have been tried and tested for achieving a successful outcome. Revascularisation is one among these treatment options, which is gaining widespread attention among endodontists. The growing body of evidence demonstrating the success of revascularisation has led to different variations of this treatment option. Clinicians have over time used different scaffolds such as blood clot, collagen, platelet-rich fibrin (PRF) and platelet-rich plasma for revascularisation. This case report outlines the management of immature maxillary central incisors with pulp necrosis and large periapical lesions in a 19-year-old female patient with a modified technique of revascularisation by combining PRF and blood clot. At the end of 12 months, the patient was completely asymptomatic along with regression of the periapical lesions.

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Obtention of injectable platelets rich-fibrin (i-PRF) and its polymerization with bone graft: technical note

Revista do Colégio Brasileiro de Cirurgiões ◽

10.1590/0100-69912015006013 ◽

2015 ◽

Vol 42 (6) ◽

pp. 421-423 ◽

Cited By ~ 27

Author(s):

Carlos Fernando de Almeida Barros Mourão ◽

Helder Valiense ◽

Elias Rodrigues Melo ◽

Natália Belmock Mascarenhas Freitas Mourão ◽

Mônica Diuana-Calasans Maia

Keyword(s):

Bone Graft ◽

Platelet Rich Plasma ◽

Technical Note ◽

Platelet Concentrates ◽

Soft Tissue Healing ◽

Liquid Form ◽

Platelet Rich Fibrin ◽

Tissue Healing ◽

Autologous Platelet ◽

Injectable Form

The use of autologous platelet concentrates, represent a promising and innovator tools in the medicine and dentistry today. The goal is to accelerate hard and soft tissue healing. Among them, the platelet-rich plasma (PRP) is the main alternative for use in liquid form (injectable). These injectable form ofplatelet concentrates are often used in regenerative procedures and demonstrate good results. The aim of this study is to present an alternative to these platelet concentrates using the platelet-rich fibrin in liquid form (injectable) and its use with particulated bone graft materials in the polymerized form.

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