scholarly journals Evaluation of Ischemia-Modified Albumin, Malondialdehyde, and Advanced Oxidative Protein Products as Markers of Vascular Injury in Diabetic Nephropathy

2016 ◽  
Vol 11 ◽  
pp. BMI.S39053 ◽  
Author(s):  
Afzal Ahmad ◽  
Poornima Manjrekar ◽  
Charu Yadav ◽  
Ashish Agarwal ◽  
Rukmini Mysore Srikantiah ◽  
...  
Keyword(s):  
Diabetic Nephropathy ◽  
Vascular Injury ◽  
Characteristic Curve ◽  
Enzyme Linked Immunosorbent Assay ◽  
Albumin Creatinine Ratio ◽  
Ischemia Modified Albumin ◽  
Urine Albumin ◽  
Significant Difference ◽  
Study Population ◽  
Diabetes Patients

AIM This study aimed at evaluation of ischemia-modified albumin (IMA), malondialdehyde (MDA), and advanced oxidative protein products (AOPP) as markers of vascular injury in diabetic nephropathy (DN) with derivation of cutoff values for the same. Materials and Methods Study population comprised 60 diabetes patients and 30 controls, with diabetes patients further categorized into three groups based on urine albumin/creatinine ratio (UACR) of <30 mg/g (diabetes without microalbuminuria), 30–300 mg/g (early DN), and >300 mg/g of creatinine (overt DN). Serum IMA, MDA, and AOPP were estimated by enzyme-linked immunosorbent assay; HbA1c, serum creatinine, urine albumin, and urine creatinine were estimated using automated analyzers. Statistical analysis was done using analysis of variance, Pearson's correlation coefficient, and receiver-operating characteristic curve. Results A statistically significant difference was found in the levels of IMA among patients with early DN (154 ng/mL), diabetes without nephropathy (109.4 ng/mL), and healthy controls (45.7 ng/mL), with highest levels in early DN cases. Similar increase was seen in AOPP as well. A significant correlation was observed between IMA and UACR in diabetes without nephropathy ( r = 0.448). Conclusion The present study postulates serum IMA as a novel biomarker for the assessment of disease progression in diabetes even before microalbuminuria, and a cutoff point ≥99 ng/mL can be used for detection of early DN.

scholarly journals The Expression of miR-192 and Its Significance in Diabetic Nephropathy Patients with Different Urine Albumin Creatinine Ratio

2016 ◽  
Vol 2016 ◽  
pp. 1-6 ◽  
Author(s):  
Xiaoyu Ma ◽  
Canlu Lu ◽  
Chuan Lv ◽  
Can Wu ◽  
Qiuyue Wang
Keyword(s):  
Diabetic Nephropathy ◽  
Transforming Growth Factor ◽  
Pearson Correlation ◽  
Linear Regression Analysis ◽  
Enzyme Linked Immunosorbent Assay ◽  
Control Group ◽  
Creatinine Ratio ◽  
Albumin Creatinine Ratio ◽  
Urine Albumin ◽  
Urine Albumin Creatinine Ratio

Objective. To investigate the expression of miR-192 and its significance in diabetic nephropathy (DN) patients.Methods. 464 patients with type 2 diabetes mellitus (T2DM) were divided into normal albuminuria group (NA,n=157), microalbuminuria group (MA,n=159), and large amount of albuminuria group (LA,n=148). 127 healthy persons were selected as the control group (NC,n=127). The serum miR-192 levels were detected by Real-Time PCR and transforming growth factor-β1 (TGF-β1) and fibronectin (FN) were detected by enzyme-linked immunosorbent assay. The relationships among these parameters were analyzed by Pearson correlation analysis and multiple linear regression analysis.Results. The miR-192 in the LA group was significantly lower than other groups, which was lower in the MA group than in the NA group (P<0.01). The TGF-β1 and FN in the LA group were significantly higher than other groups, which were higher in the MA group than in the NA group (P<0.01). The expression of miR-192 was negatively correlated with TGF-β1, FN, and Ln (UACR) and miR-192, TGF-β1, and FN were independent relevant factors affecting Ln (UACR) in T2DM (P<0.01).Conclusions. These findings indicate that the levels of miR-192 were lower accompanied by the decrease of urine albumin creatinine ratio (UACR) and the association between miR-192 and nephritic fibrosis in DN.

scholarly journals Comparison of urine albumin creatinine ratio with the pediatric index of mortality 2 score for prediction of pediatric intensive care unit outcomes

2021 ◽  
Author(s):  
Shifa Nismath ◽  
Suchetha S. Rao ◽  
B. S. Baliga ◽  
Vaman Kulkarni ◽  
Gayatri M. Rao
Keyword(s):  
Intensive Care Unit ◽  
Intensive Care ◽  
Pediatric Intensive Care Unit ◽  
Characteristic Curve ◽  
Pediatric Intensive Care ◽  
Creatinine Ratio ◽  
Albumin Creatinine Ratio ◽  
Urine Albumin ◽  
Urine Albumin Creatinine Ratio ◽  
Pediatric Index Of Mortality

Abstract Background Predicting morbidity and mortality in a pediatric intensive care unit (PICU) is of extreme importance to make precise decisions for better outcomes. Aim We compared the urine albumin creatinine ratio (ACR) with the established PICU score, pediatric index of mortality 2 (PIM 2) for predicting PICU outcomes. Methods This cross-sectional study enrolled 67 patients admitted to PICU with systemic inflammatory response syndrome. Urine ACR was estimated on admission, and PIM 2 score was calculated. ACR was compared with PIM 2 for PICU outcome measures: the need for inotropes, development of multiple organ dysfunction syndrome (MODS), duration of PICU stay, and survival. Results Microalbuminuria was found in 77.6% of patients with a median ACR of 80 mg/g. ACR showed a significant association with the need for inotropes (p < 0.001), MODS (p = 0.001), and significant correlation to PICU stay (p 0.001, rho = 0.361). The area under the receiver operating characteristic curve for ACR (0.798) was comparable to that of PIM 2 (0.896). The cutoff value of ACR derived to predict mortality was 110 mg/g. The study subjects were divided into 2 groups: below cutoff and above the cutoff. Outcome variables, inotrope use, MODS, mortality, and PICU stay compared between these subgroups, were statistically significant. Conclusion ACR is a good predictor of PICU outcomes and is comparable to PIM 2 for mortality prediction.

Diagnostic efficacy of serum and urinary netrin-1 in the early detection of diabetic nephropathy

2021 ◽  
pp. jim-2021-001785
Author(s):  
Rasha A Elkholy ◽  
Reham L Younis ◽  
Alzahraa A Allam ◽  
Rasha Youssef Hagag ◽  
Muhammad Tarek Abdel Ghafar
Keyword(s):  
Diabetic Nephropathy ◽  
Early Detection ◽  
Characteristic Curve ◽  
Fasting Blood Glucose ◽  
Area Under The Curve ◽  
Diagnostic Value ◽  
Control Group ◽  
Diagnostic Efficacy ◽  
Significant Difference ◽  
Patients With Diabetes

This study aimed to assess the diagnostic value of serum and urinary netrin-1 in patients with type 2 diabetes mellitus (T2DM) at different stages of diabetic nephropathy (DN) and to compare its efficacy of estimation in serum with that in the urine. This study was carried out on 135 patients with T2DM and 45 healthy subjects. The patients with diabetes were divided according to urinary albumin creatinine ratio (UACR) into: T2DM with normoalbuminuria, incipient DN with microalbuminuria, and overt DN with macroalbuminuria groups. Serum and urinary levels of netrin-1 were measured by ELISA. The mean levels of serum and urinary netrin-1 were significantly higher in the microalbuminuric and macroalbuminuric patients with DN than those in the normoalbuminuric patients with T2DM, with the highest values detected in macroalbuminuric patients with DN. Urinary netrin-1 level was significantly higher in the normoalbuminuric T2DM group than control group, whereas no significant difference existed regarding serum netrin-1 level. In T2DM groups, the urinary and serum netrin-1 correlated with each other and were independently related to fasting blood glucose, UACR, and estimated glomerular filtration rate. Receiver operating characteristic curve analysis showed that the area under the curve of urinary netrin-1 was 0.916 which is significantly higher than that of serum netrin-1 (0.812) for the detection of incipient DN and reached 0.938 on coestimation of both urinary and serum netrin-1. In conclusion, netrin-1 is a potential diagnostic marker for early detection of DN with its estimation in urine has higher accuracy than that of serum.

scholarly journals The Role of Ischemia-modified Albumin as a Biomarker in Preeclampsia

2020 ◽  
Vol 42 (03) ◽  
pp. 133-139
Author(s):  
Süleyman Serkan Karaşin ◽  
Tayfur Çift
Keyword(s):  
Pregnant Women ◽  
Laboratory Data ◽  
Cross Sectional Study ◽  
Enzyme Linked Immunosorbent Assay ◽  
Cross Sectional ◽  
Ischemia Modified Albumin ◽  
Significant Difference ◽  
Gestational Week ◽  
Severity Of The Disease

Abstract Objective Ischemia-modified albumin (IMA)is a modified type of albumin protein that is formed under oxidative stress. This study aims to compare the levels of serum IMA between normotensive and preeclamptic pregnancies and to evaluate the relationship between the severity of the disease. Methods A total of 90 pregnant women aged between 18 and 45 years participated in this cross-sectional study. The levels of serum IMA were measured by enzyme-linked immunosorbent assay in 30 preeclamptic pregnant women with the severe signs of the disease, 30 preeclamptic pregnant women, and 30 normotensive pregnant women.. The study was designed as a cross-sectional clinical study. Results When the demographic characteristics were examined, statistically significant differences were found between the groups in terms of age, gestational week at birth and blood pressure. Age was higher in the preeclampsia with signs of severity group than in the normotensive group (p = 0.033). Pregnancy week was significantly the lowest in the preeclampsia with the severity signs group (p = 0.004). In normotensive patients, IMA levels were lower than in the preeclampsia groups (p < 0.001) but there was no significant difference in terms of severity of disease (p = 0.191). According to laboratory data; only the creatinine level was significantly different between the groups. Conclusion The levels of serum IMA were higher in patients with preeclampsia than in healthy pregnancies. However, there was no significant correlation in terms of preeclampsia severity; more extensive, prospective and long-term studies are needed.

Microalbuminuria Measured by three Different Methods, Blood Pressure and Cardiovascular Risk Factors in Elderly Swedish Males

2008 ◽  
Vol 3 ◽  
pp. 117739010800300 ◽  
Author(s):  
Gösta Florvall ◽  
Samar Basu ◽  
Johanna Helmersson ◽  
Anders Larsson
Keyword(s):  
Blood Pressure ◽  
Diastolic Blood Pressure ◽  
Creatinine Ratio ◽  
Cross Sectional ◽  
Albumin Creatinine Ratio ◽  
Mortality And Morbidity ◽  
Amyloid A ◽  
Urine Albumin ◽  
Similar Information ◽  
Study Population

Microalbuminuria is associated with hypertension and is a strong risk factor for subsequent chronic disease, both renal and coronary heart disease (CHD), Presently there are several methods available for measurement of microalbuminuria. The aim of this study was to evaluate if the three different methods gave similar information or if one of the assays were superior to the others. Blood pressure, inflammatory markers and cardiovascular mortality and morbidity were correlated with urine albumin analysed with a point-of-care testing (POCT) instrument, nephelometric determination of albumin and albumin/creatinine ratio in elderly males. The study population consisted of 103 diabetic and 603 nondiabetic males (age 77 years) in a cross-sectional study. We analyzed urine albumin with a HemoCue® Urine Albumin POCT instrument and a ProSpec® nephelometer and albumin/creatinine ratio. There were strong correlations between both systolic and diastolic blood pressure and all three urine albumin methods (p < 0.0001). There were also significant correlations between the different urine albumin measurements and serum amyloid A component, high-sensitivity C-reactive protein and interleukin-6. The three different urine albumin methods studied provided similar information in relation to cardiovascular disease. There was a strong correlation between systolic and diastolic blood pressure and microalbuminuria in both the whole study population and in nondiabetic males emphasizing the role of hypertension in glomerular damage. The good correlation between the studied urine albumin measurements show that all three methods can be used for monitoring urine albumin excretion.

scholarly journals Genetic Relationship Between Interleukın-6 rs1800796 Variants,  Interleukın-6 Plasma Levels and Susceptibility to Type 2 Diabetes Mellitus and Diabetic Nephropathy

2021 ◽  
Author(s):  
Nezaket COBAN ◽  
Aysegul Bayramoglu ◽  
Zeynep TEMIZ
Keyword(s):  
Diabetes Mellitus ◽  
Type 2 Diabetes ◽  
Type 2 Diabetes Mellitus ◽  
Diabetic Nephropathy ◽  
Inflammatory Cytokines ◽  
Interleukin 6 ◽  
Enzyme Linked Immunosorbent Assay ◽  
Control Group ◽  
Significant Difference

Abstract Type 2 diabetes mellitus (T2DM) is very common worldwide and genetically heterogeneous. One of the microvascular complications is diabetic nephropathy (DN). In recent years, T2DM has been described as a disease caused by chronic inflammation. The imbalance between pro- and anti-inflammatory cytokines causes inflammation. One of the candidate genes associated with T2DM and DN is the Interleukin-6 (IL-6) gene, one of the pro-inflammatory cytokines. This study was conducted to determine the polymorphism frequencies of the IL-6 gene rs1800796 and investigate the role of this polymorphism in the development of T2DM and DN. Genomic DNA that was obtained from 261 people was used in the study. IL-6 gene rs1800796 polymorphism was determined using the PCR, restriction fragment length polymorphism (RFLP) and electrophoresis. IL-6 gene PCR products were discontinued by treatment with restriction enzyme BsrBI and were analyzed in 2% agarose gel electrophoresis. IL-6 (Bioassay technology laboratory, Shangai, China) level was examined by enzyme-linked immunosorbent assay (ELISA) using a commercial kit. The results were statistically analyzed. The frequencies of rs1800796 genotypes were found to be GG 70.7%, GC 28.5%, CC 0.8% in the control group and GG 87.8%, GC 9.9 %, CC 2.3% in T2DM patients. Although there was a statistically significant difference between the control group and the T2DM patient group in genotype and allele frequencies, there was no significant difference in DN. The G allele frequency was also significantly higher in the T2DM group (p=0.000). IL-6 levels were determinated increased in patients with Type-2 diabetes compared to the control group. However; there was no significant statistically. We can say that IL-6 rs1800796 polymorphism is related to T2DM and G allele can be used as a useful genetic marker; this polymorphism is not related to DN, though.

Study of Ischemia-Modified Albumin as Marker of Vascular Injury in Diabetic Nephropathy

2021 ◽  
pp. 45-55
Author(s):  
Asmaa Hassan Abd-Elaziz ◽  
Hossam Abd-Elmohsein Hodeib ◽  
Abeer Abd-El Moneim Shahba ◽  
Amal Selim
Keyword(s):  
Diabetic Nephropathy ◽  
Glomerular Filtration Rate ◽  
Glomerular Filtration ◽  
Vascular Injury ◽  
Filtration Rate ◽  
Tertiary Structure ◽  
Control Group ◽  
P Value ◽  
Ischemia Modified Albumin ◽  
Group I

Background: Diabetes mellitus (DM) is strongly correlated to ischemia caused by stress that is involved in dysfunction of endothelium leading to angiopathy, particularly diabetic nephropathy (DN). Immediately after occlusion of an artery, free radical production paired with reduced antioxidant defensive response and related ischemia in DM result in changes in the tertiary structure of human serum albumin; therefore, it is called ischemia-modified albumin (IMA). The aim of our work was to evaluate IMA as marker of vascular injury in DN. Patients and Methods: This trial was conducted on 25 normal individuals as a control group (I) and 75 cases with type II DM. Cases have been subdivided into Group II: 25 diabetic cases with normal albumin in urine, Group III: 25 diabetic cases with albumin in urine and glomerular filtration rate >60 ml/min/1.73m2 and Group IV: 25 diabetic cases with albuminuria and glomerular filtration rate <60ml/min/1.73m2. Medical history, clinical features and laboratory investigations which include the serum IMA have been carried out to all cases. Results: Compared to the control group, considerably greater serum levels of IMA have been reported in Diabetic cases there had been a wide variation in IMA among the 4 groups. There were considerable +ve correlations among serum IMA and DM duration, blood urea, serum creatinine, UACR, FBS, PPBS and HBA1c. A considerable -ve correlation among serum IMA and eGFR was observed. IMA had an optimal cutoff value≥90 ng/ml with 0.92 area under ROC curve, 96% sensitivity and 92% specificity (p-value<0.001). Conclusion: IMA could function as a glycemic control marker and a sensitive indicator for DN.

scholarly journals Urine-albumin and creatinine ratio among apparently healthy individual in South Eastern, Nigeria

2021 ◽  
Vol 16 (1) ◽  
pp. 105-114
Author(s):  
Amos Dangana ◽  
Ifeoma Miracle Okoronkwo ◽  
Solomon Oloche Onoja ◽  
Innocent Nwabueze Okonkwo ◽  
Nonye Bibiana Egenti ◽  
...  
Keyword(s):  
Healthy Individual ◽  
Kinetic Method ◽  
Pulse Height ◽  
Calculated Data ◽  
Enzyme Linked Immunosorbent Assay ◽  
Creatinine Ratio ◽  
Hypertensive Nephropathy ◽  
Urine Albumin ◽  
Significant Difference ◽  
Apparently Healthy

Background: Albuminuria and Albumin-Creatinine Ratio (ACR) has been identified as early indices of cardiovascular diseases (CVD), Hypertension and are at great risk of hypertensive nephropathy, but this not so for the apparently healthy individuals. Objectives: This study was therefore carried out to evaluate and quantitate albuminuria and urine ACR among apparently healthy individual and to compare values with the hypertensive attending University of Nigeria Teaching Hospital, Ituku-Ozalla Enugu State. Materials and method: The study was carried out on eighty-nine (89) volunteers (50 tests and 39 controls) age 21-77 years between August and September 2019. A structured questionnaire was used to get vital information, anthropometric measurements were taken while blood and urine samples were collected and analyzed for serum creatinine (Scr), urine creatinine (UCr) and urine albumin using Jaffe modified kinetic method and Enzyme Linked Immunosorbent Assay (ELISA) method respectively. Body mass index (BMI) in Kg/m2, urine ACR in mg/mmol and estimated glomerular filtration rate (eGFR) using Chronic Disease Epidemiology Equation were calculated. Data were analyzed using student’s T-test and one-way Analysis of variance (ANOVA). Results: Generally, results showed that males have higher (p<0.05) height, Scr, eGFR, and UCr but lower (p<0.05) BMI) when compared with the females, but no significant difference (P>0.05) between systolic blood pressure (SBP), diastolic BP (DBP), pulse, weight, urine albumin and ACR. Also, the study revealed that the test subjects have higher SBP, DBP, weight, BMI, SCr, urine albumin, and ACR but lower (p<0.05) eGFR and UCr when compared with the control subjects, but no significant difference (P>0.05) between pulse and height. Furthermore, intra gender analysis of the results showed that males tests have higher SBP, DBP, weight, BMI and urine ACR but lower (p<0.05) eGFR and UCr when compared with male controls, but no significant difference (P>0.05) was observed for pulse, height, Scr and urine albumin. Finally, the results showed that female tests have higher (P<0.05) SBP, pulse, Scr, urine albumin and ACR but lower (p<0.05) eGFR when compared with the female controls Conclusion: Findings and results from this study suggest that the hypertensives stand a higher risk of kidney damage, consequently cardiovascular disease (CVD).

Significance of urine albumin / creatinine ratio (UACR) and uric acid in women with preeclampsia and its comparison with healthy normotensive pregnant women in their third trimester

2014 ◽  
Vol 21 (1) ◽  
pp. 9-15
Author(s):  
Sonal Sogani ◽  
Vandana Varma ◽  
Purnima Dey Sarkar
Keyword(s):  
Uric Acid ◽  
Pregnant Women ◽  
Creatinine Ratio ◽  
Third Trimester ◽  
Albumin Creatinine Ratio ◽  
Mean Values ◽  
Urine Albumin ◽  
Significant Difference ◽  
Urine Albumin Creatinine Ratio ◽  
New Onset

Objective. To determine the significance of urine albumin / creatinine ratio (UACR) as to predict proteinuria in new-onset hypertension and uric acid in women with preeclampsia and its comparison with healthy normotensive pregnant women. Methods. Healthy normotensive pregnant women (n = 45), women with preeclampsia (n = 36) were included in the study. The preeclamptic group was further divided into two subgroups: mild (n  =  25) and severe preeclampsia (n = 11). Results. Higher mean values of urine albumin / creatinine ratio (UACR) and uric acid were found in preeclamptic pregnant women (319.05 ± 247.56 mg/g, 5.98  ±  2.1  mg/dl) as compared to healthy normotensive pregnant women (22.15  ±  8.1  mg/g, 4.07  ±  0.91  mg/dl) in the third trimester with highly significant difference (p 

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