Effect of mechanical processing of corn silage on in vitro ruminal fermentation, and in situ bacterial colonization and dry matter degradation

2007 ◽  
Vol 87 (2) ◽  
pp. 259-267 ◽  
Author(s):  
K. Kozakai ◽  
T. Nakamura ◽  
Y. Kobayashi ◽  
T. Tanigawa ◽  
I. Osaka ◽  
...  
Keyword(s):  
Dry Matter ◽  
Bacterial Colonization ◽  
Rumen Fermentation ◽  
Corn Silage ◽  
Rumen Bacteria ◽  
Mechanical Processing ◽  
Copy Numbers ◽  
Rdna Copy

Rumen fermentation and bacterial colonization of mechanically processed corn silage were evaluated in a series of in vitro and in situ experiments using ruminally cannulated cows. Data from rumen-simulated continuous culture (Rusitec) showed that total VFA concentration was increased (P < 0.001) by feeding processed corn silage, while pH was reduced (P < 0.001) and ammonia nitrogen remained unchanged (P > 0.05). Mechanical processing enhanced the in situ rate and extent of dry matter disappearance for corn stems and kernels, respectively (P < 0.05). It also enhanced in situ, the initial attachment of rumen bacteria responsible for corn digestion, as monitored by real-time PCR. The16S rDNA copy numbers of amylolytic and lipolytic bacteria, such as Succinivibrio dextrinosolvens, Ruminobacter amylophilus and Anaerovibrio lipolytica, attached to corn kernels were increased for processed corn silage (P < 0.05), in particular at 2 h after the start of incubation. In addition, corn stemattached cellulolytic and hemicellulolytic bacteria, such as Fibrobacter succinogenes, ruminococci and Eubacterium ruminantium, increased in 16S rDNA copy numbers for processed corn silage (P < 0.05). This was observed for the initial incubation period from 10 min to 2 h. These results demonstrate more rapid attachment and heavier colonization of rumen bacteria to the processed compared with the unprocessed silage, which facilitates ruminal digestion and fermentation. Key words: Corn silage, mechanical processing, rumen fermentation, bacteria, attachment

scholarly journals Effects of Microalgae Species on In Vitro Rumen Fermentation Pattern and Methane Production

2020 ◽  
Vol 20 (1) ◽  
pp. 207-218 ◽  
Author(s):  
Ekin Sucu
Keyword(s):  
Methane Production ◽  
Dry Matter ◽  
Volatile Fatty Acids ◽  
Rumen Fermentation ◽  
Gas Production ◽  
Corn Silage ◽  
Fermentation Pattern ◽  
Proximate And Mineral Composition

AbstractThis experiment was conducted to establish the effects of two types of microalgae [Chlorella vulgaris (AI), C. variabilis (AII) and their combination (AI+AII)] with two substrates (wheat and corn silages) on rumen fermentation, gas and methane production. To each substrate, one of 3 algae treatment was supplemented at 0% and 25% of the total incubated dry matter. A series of 5 measurement points (3, 6, 12, 24 and 48 h) were completed and the gas production was monitored. The proximate and mineral composition of microalgae and substrates were examined. At 48 h incubation rumen fermentation variables and CH4 production were also assessed. When compared with wheat silage, corn silage caused an increase in gas production (P<0.05). Ruminal gas production decreased in the algae groups when compared to the controls (0% algae, wheat and corn silages, P<0.05). Among algae, C. vulgaris had the strongest effect, decreasing gas production by 34%. Among algae, the total volatile fatty acids (VFA) and CH4 production were found to be lower in C. variabilis (P<0.001). Ammonia-N increased with the algae inclusion (P<0.05). But, the ruminal gas production, pH, acetate, the total VFA, CH4 and rumen fermentation efficiency were not affected by the substrate and algae interaction (P>0.05). The propionate was the highest (P<0.05) for corn silage when incubated with C. vulgaris. Ruminal butyrate was the lowest for the wheat silage when incubated with the mixture of algae (P<0.05). The NH3-N was the highest in corn silage when incubated with all algae types (P<0.05). Careful selection and combination of substrate and algae may positively manipulate rumen fermentation and may inhibit CH4 production. Further research is needed to validate these results in vivo.

PSXIII-4 Effects of “País” grape marc on in vitro methane production and ruminal fermentation parameters of a forage diet

2021 ◽  
Vol 99 (Supplement_3) ◽  
pp. 468-468
Author(s):  
Sandra Suescun-Ospina ◽  
Nelson Vera ◽  
Rita Astudillo ◽  
Jorge Avila-Stagno
Keyword(s):  
Methane Production ◽  
Dry Matter ◽  
Rumen Fermentation ◽  
Gas Production ◽  
Corn Silage ◽  
Partial Substitution ◽  
Grape Marc ◽  
Ch4 Production ◽  
Fermentation Parameters

Abstract Grape marc (GM) is a viticulture by-product used as cattle supplement in periods of shortage of conventional feed sources. It contains fats, high concentrations of polyphenols and has been reported to reduce enteric methane (CH4) emissions. In-vitro batch culture was used to study the effects of substitution of mixed hay (MH) for a traditional Chilean variety (Vitis vinifera “País”) of GM on in vitro dry matter disappearance (IVDMD), rumen fermentation parameters (short chain fatty acids, pH, partitioning factor), gas and CH4 production in a 60% forage diet (dry matter, DM). The study was a randomized complete design with 3 treatments and 3 replicates, incubated for 24 h at 39º C. Treatments were: T1 (Control): 20% MH, 40% corn silage, 40% concentrate; T2 = 10% MH, 10% GM, 40% corn silage, 40% concentrate; T3 = 20% GM, 40% corn silage, 40% concentrate. Means were compared with the Tukey test (P &lt; 0.05), and polynomial contrasts. Substitution of MH with GM significantly reduced ammonia nitrogen (NH3-N) by 50% (P &lt; 0.05), although it did not affect IVDMD, gas production or other rumen fermentation parameters (P &gt; 0.05). Total CH4 (mg) linearly decreased (P = 0.013) as concentrations of GM increased. Methane production (mg/g DM incubated) and yield (mg/g DM digested) decreased linearly (P = 0.002 and P = 0.003, respectively) as inclusion of GM increased. Inclusion of GM at 20% reduced CH4 production by 19% and CH4 yield by 16.4%. These results indicate that partial substitution of dietary fiber sources with traditional Chilean País GM in high fiber diets is a viable feeding alternative, and can decrease environmental impact (lower CH4 and ammonia emissions) of ruminant livestock, without negatively affecting rumen fermentation parameters.

scholarly journals Evaluation of the Nutrient Composition, In Vitro Fermentation Characteristics, and In Situ Degradability of Amaranthus caudatus, Amaranthus cruentus, and Amaranthus hypochondriacus in Cattle

Animals ◽  
2020 ◽  
Vol 11 (1) ◽  
pp. 18
Author(s):  
Kim Margarette C. Nogoy ◽  
Jia Yu ◽  
Young Gyu Song ◽  
Shida Li ◽  
Jong-Wook Chung ◽  
...  
Keyword(s):  
Crude Protein ◽  
Dry Matter ◽  
Gas Production ◽  
Nutrient Composition ◽  
Neutral Detergent Fiber ◽  
Amaranthus Cruentus ◽  
Amaranthus Caudatus ◽  
Amaranthus Hypochondriacus

The amaranth plants showed high potential feed value as forage for ruminants. An in-depth study of this plant, particularly in cattle, will help extend its utilization as an alternative protein and fiber feed source in cattle feeding. In this study, the nutrient compositions of three different species of amaranth, Amaranthus caudatus L., Amaranthus cruentus L., and Amaranthus hypochondriacus L.—two varieties for each species, A.ca 74, A.ca 91, A.cu 62, A.cu 66, A. hy 30, and A. hy 48—were evaluated. The in vitro technique was used to evaluate the fermentation characteristics such as total gas production, total volatile fatty acids (VFA) concentration, pH, and ammonia concentration of the rumen fluid. Moreover, the effective degradabilities of dry matter (EDDM) and crude protein (EDCP) of the amaranth forages were determined through in situ bag technique. The amaranth forages: A. caudatus, A. cruentus, and A. hypochondriacus showed better nutritive value than the locally produced forages in Chungcheong province of Korea. The CP of the amaranth ranged from 11.95% to 14.19%, and the neutral detergent fiber (NDF) and acid detergent fiber (ADF) contents ranged from 45.53% to 70.88% and 34.17% to 49.83%, respectively. Among the amaranth varieties, A. hypochondriacus 48 showed the most excellent ruminant feed nutrient quality (CP, 14.19%; NDF, 45.53%; and ADF, 34.17%). The effective degradabilities of dry matter (EDDM; 33–56%) and crude protein EDCP (27–59%) of the amaranth were lower compared to other studies, which could be due to the maturity stage at which the forages were harvested. Nonetheless, A. hypochondriacus 48 showed the highest EDDM (56.73%) and EDCP (59.09%). The different amaranth species did not differ greatly in terms of total VFA concentration or molar proportions, total gas production, or ammonia-N concentration. The high nutrient composition, and highly effective degradability of dry matter and crude protein, coupled with the favorable fermentation characteristics, suggest that the amaranth forages showed good to excellent feed quality for cattle.

PSXIV-6 País grape marc (Vitis vinifera, País) inclusion in concentrate diets reduces ammonia nitrogen but not methane production in an in vitro batch culture

2021 ◽  
Vol 99 (Supplement_3) ◽  
pp. 469-470
Author(s):  
Sandra Suescun-Ospina ◽  
Nelson Vera ◽  
Rita Astudillo ◽  
Jorge Avila-Stagno
Keyword(s):  
Vitis Vinifera ◽  
Dry Matter ◽  
Substantial Reduction ◽  
Ammonia Nitrogen ◽  
Corn Silage ◽  
Wine Industry ◽  
Fermentation Efficiency ◽  
Grape Marc ◽  
Ch4 Production

Abstract País Grape (Vitis vinifera L.) is an ancestral variety used in Chilean wine industry. It has a higher content of proanthocyanidins than commercial varieties such as Carmenère or Pinot Noir, resulting in País grape marc (PGM) with high contents of condensed tannins. As such, PGM inclusion in ruminant diets would have the potential to reduce enteric methane (CH4) emissions and decrease urinary N excretion. The objective of this study was to evaluate the effects of substitution of mixed hay (MH) with PGM in a high concentrate diet [65% dry matter (DM)] on in vitro dry matter disappearance (IVDMD), ruminal fermentation parameters, gas and CH4 production. Treatments were: T1 (Control) = 20% MH, 15% corn silage, 65% concentrate; T2 = 10% MH, 10% PGM, 15% corn silage, 65% concentrate; T3 = 20% PGM, 15% corn silage, 65% concentrate. The study was a randomized complete design with 3 treatment and 3 replicates, incubated for 24 h at 39º C. Data were compared by Tukey test and polynomial contrasts. There was a linear reduction in NH3-N (P = 0.001) as dietary PGM increased. Inclusion of PGM reduced NH3-N by 50% when added at 10% DM, and 71.7% at 20% DM. However, there also was 4% reduction in IVDMD (P ≤ 0.001) and gas production (P = 0.012) in the 20% GM diet. There were no treatment effects (P ≥ 0.05) on CH4 production or yield. Fermentation efficiency determined by the partition factor increased linearly (P = 0.013) as PGM inclusion increased, suggesting that it increases organic matter to be degraded. Based on this study it could be concluded that PGM is an alternative source of fiber for ruminants on concentrate diets, as it can result in improved rumen fermentation efficiency and a substantial reduction in ruminal ammonia nitrogen concentration (NH3-N).

Influence of single-gene mutations, harvest maturity and sample processing on ruminal in situ and post-ruminal in vitro dry matter and starch degradability of corn grain by ruminants

2009 ◽  
Vol 151 (3-4) ◽  
pp. 240-250 ◽  
Author(s):  
D. Ngonyamo-Majee ◽  
R.D. Shaver ◽  
J.G. Coors ◽  
D. Sapienza ◽  
J.G. Lauer
Keyword(s):  
Dry Matter ◽  
Single Gene ◽  
Gene Mutations ◽  
Sample Processing ◽  
Harvest Maturity ◽  
Corn Grain

Ruminal degradation characteristics of corn and corn–sunflower intercropped silages prepared at two stages of maturity

1992 ◽  
Vol 72 (4) ◽  
pp. 881-889 ◽  
Author(s):  
Z. Mir ◽  
P. S. Mir ◽  
S. Bittman ◽  
L. J. Fisher
Keyword(s):  
Dry Matter ◽  
Degradation Rate ◽  
Soluble Fraction ◽  
Corn Silage ◽  
Neutral Detergent Fiber ◽  
Acid Detergent Fiber ◽  
Ruminal Degradation ◽  
Whole Plants ◽  
Two Stages

The degradation characteristics of dry matter (DM), protein, neutral detergent fiber (NDF) and acid detergent fiber (ADF) of corn–sunflower intercrop silage (CSS) and monoculture corn silage (CS) prepared from whole plants, harvested at two stages of maturity, were compared using eight mature rumen-cannulated steers. The degradation characteristics were determined by incubating the silages in nylon bags for up to 72 h in the rumen of animals fed the respective silages. The degradation characteristics determined for the silages were the soluble fraction, the insoluble but degradable fractions, the rate of degradation of the degradable fractions of silage DM and protein, and the in situ disappearance of NDF and ADF after 0.5 and 72 h of incubation. The rate of particulate passage from the rumen was determined using chromium-mordanted NDF of the four silages. Values were used to estimate effectively degraded DM and protein. The rates of DM and protein degradation were highest for late-cut CSS (6.3 and 6.0% h−1, respectively) and the least for late-cut CS (2.5 and 0.8% h−1, respectively). Averaged across stages of maturity, more (P < 0.05) DM and protein were effectively degraded with CSS (57.4 and 70.1%, respectively) than with CS (48.8 and 48.7%). Degradation of NDF in early-cut CSS was lower (P < 0.05) than in CS after 72 h of incubation. ADF disappearance from all of the silages after 72 h of incubation was similar. Ruminal degradation of DM and protein in CSS was greater than in CS, which may affect efficiency of utilization of CSS. Key words: Degradation rate, effective degradability, corn silage, intercropped corn–sunflower, steers

1435 Lactation performance, in situ degradability, and rumen fermentation of Holstein cows fed BMR-6 sorghum silage versus corn silage based diets

2016 ◽  
Vol 94 (suppl_5) ◽  
pp. 696-696
Author(s):  
K. K. Gautam ◽  
S. J. Trojan ◽  
J. O. Sarturi ◽  
M. A. Ballou
Keyword(s):  
Rumen Fermentation ◽  
Corn Silage ◽  
Holstein Cows ◽  
Lactation Performance ◽  
Sorghum Silage

scholarly journals Degradación microbiana in situ de tejidos foliares de gramíneas y leguminosas y su relación con indicadores de calidad nutricional

2005 ◽  
Vol 6 (1) ◽  
pp. 100
Author(s):  
Diego Chamorro ◽  
Juan Evangelista Carulla ◽  
Pablo Cuesta
Keyword(s):  
Nutritional Quality ◽  
Dry Matter ◽  
Neutral Detergent Fiber ◽  
Dry Matter Degradation ◽  
Leaf Tissues ◽  
Dry Seasons ◽  
Stylosanthes Scabra ◽  
Negative Relationships

<p>Para cuantificar la degradación <em>in situ </em>de tejidos vegetales y su relación con la composición química de especies forrajeras se seleccionaron láminas foliares de las gramíneas <em>Bouteloua repens </em>y <em>Bothriochloa pertusa </em>y foliolos de las leguminosas <em>Stylosanthes scabra</em>, <em>Desmodium barbatum </em>y <em>Tephrosia cinerea</em>. En el rumen se incuba­ron muestras de 10 mm de largo durante 0, 12, 24, 48 y 72 horas; para la lectura del área residual se digitalizaron ocho tejidos. A las 24 y 48 h de incubación <em>B. pertusa </em>presentó relaciones positivas entre la degradación de la epidermis adaxial (EA) y la FDN (R2= 90,2), entre el mesófilo y la DIVMS (R2=80,1), y entre los tejidos len­tamente degradables (TLD) y la FDA (R2= 83,9); y relaciones negativas entre EA y DIVMS (R2= –73,1), los TLD con la DEF (R2= –74,3), la EA con la PC (R2= –87,6), y el esclerenquima con la DIVMS y la PC (R2= –84,3 y R2= –90,8). Después de 72 horas de incubación las mayores áreas residuales en gramíneas fueron la estructura kranz entre 34,2% y 36,5%, el mesófilo entre 20,9% y 21,4%, el xilema de 11,5% a 17,1% y la EA entre 11,8% y 13,9%; no obstante el esclerénquima se relacionó estrechamente con indicadores de calidad nutricional. Las gramíneas obtuvieron mayores porcen­tajes de tejidos no degradables que las leguminosas; por lo tanto, la DIVMS y la DEF fueron menores. Entre las leguminosas, <em>S. scabra</em>, presentó la mayor digestibilidad de los tejidos rápidamente degradables, tanto en sequía como en lluvia; en la época seca se incrementó en 134,6% la degradación de estos tejidos. Adicionalmente, en esta especie el área residual de los taninos no presentó relación directa con la degra­dación del mesófilo. En leguminosas la tasa y extensión de la degradación de las epidermis se incrementaron marcadamente a mayor tiempo de incubación, presen­tándose diferencias entre especies, situación que no exhibieron las gramíneas. En <em>D. barbatum </em>la degradación de la EA se asoció con la DIVMS y la DEF, explicando en 77,4% y 72,95% estos porcentajes; de igual manera se reportaron relaciones negati­vas entre la degradabilidad de la epidermis y los porcentajes de FDN, FDA, lignina y taninos (R2= –0,76; R2= –0,79; R2= –0,53 y R2= –0,76, respectivamente).</p><p> </p><p><strong>Microbial <em>in situ </em>degradation of grasses and legumes leaf tissues and its realtionship with nutritional quality and precipitation</strong></p><p>A study was conducted to measure the <em>in situ </em>de- gradation of plant tissues and its relationship with forage chemical composition in leaves of the grasses <em>Bouteloua repens </em>and <em>Bothriochloa pertusa </em>and of the legumes <em>Stylosanthes scabra</em>, <em>Desmodium barbatum </em>and <em>Tephrosia cinerea</em>. Forage samples of 10 mm in length were incubated in the rumen for 0, 12, 24, 48 and 72 h and tissue residual area was determined by reading the residual area in eight digitalized leaf samples per forage. In <em>B. pertusa</em>, after 24 and 48 h of incubation there were positive relations between the degradation of adaxial epidermis (AE) and the neutral detergent fiber (NDF, R2 = 90.2), between mesophyll and in vitro dry matter degradability (IVDMD, R2 = 80,1), and between slowly degradable tissues (SDT) and the acid detergent fiber (ADF, R2 = 83.9); and negative relationships between AE and IVDMD (R2 = -73,1), between SDT and effective dry matter degradation (EDMD, R2 = -74,3), between AE and crude protein (CP, R2 = -87,6), and between sclerenchyma and IVDMD with CP (R2 = -84,3 and R2 = -90,8, respectively). After 72 h of incubation, the greater residual areas in grasses were the Kranz structure (34.2% to 36.5%), mesophyll (20.9% to 21.4%), xylem (11.5% to 17.1%) and AE (11.8% to 13.9%). The area of sclerenchyma was related close­ly to indicators of nutritional quality. Overall, the grasses had greater content of non-degradable tis­sues than the legumes; therefore, their IVDMD and EDMD were lower. Among the legumes, <em>S. scabra </em>showed the greater digestibility of rapidly degrada­ble tissues, both during the rainy and dry seasons. At the dry season, the degradation of these tissues increased by 134.6%. Additionally, in this legume, the content of tannins did not have a direct relation­ship with the degradation of mesophyll. The rate and extension of the degradation of epidermis in legumes, were noticeably increased with increasing length of incubation, existing differences between species, a situation which was not observed in the grasses. In <em>D. barbatum</em>, the degradation of the AE was positively associated with both the IVDMD and EDMD (R2 = 77,4% and 72,95%, respectively). On the other hand, the degradability of epidermis and the percentage of NDF had negative relations with ADF, lignin and tannins (R2 = -0,76; R2 = -0,79; R2 = -0,53 and R2 = -0,76, respectively).</p>

scholarly journals Nutritional Quality and In Vitro Rumen Fermentation Characteristics of Silage Prepared with Lucerne, Sweet Maize Stalk, and Their Mixtures

Agriculture ◽  
2021 ◽  
Vol 11 (12) ◽  
pp. 1205
Author(s):  
Musen Wang ◽  
Fujin Zhang ◽  
Xinxin Zhang ◽  
Ying Yun ◽  
Lei Wang ◽  
...  
Keyword(s):  
Organic Matter ◽  
Nutritional Quality ◽  
Dry Matter ◽  
Rumen Fermentation ◽  
Gas Production ◽  
Neutral Detergent Fiber ◽  
Ruminal Fermentation ◽  
Fresh Matter ◽  
In Vitro Rumen Fermentation

The objective of this work was to evaluate the pH, chemical composition, minerals, vitamins, and in vitro rumen fermentation characteristics of silage prepared with lucerne, sweet maize stalk (MS), and their mixtures. Freshly chopped lucerne and MS were combined in ratios of 100:0 (M0, control), 80:20 (M20), 60:40 (M40), 40:60 (M60), 20:80 (M80), and 0:100 (M100) on a fresh matter basis. Each treatment was prepared in triplicate, and a total of eighteen silos were fermented for 65 days. After 65 days of fermentation, the pH values in M0, M20, M40, M60, M80, and M100 silages were 5.47, 4.84, 4.23, 4.13, 3.79, and 3.61, respectively. As the MS proportion in the mixtures increased, silage K, Ca, P, Na, Fe, and Cu concentrations linearly decreased (p < 0.001) and so did vitamins B5 and K1 and α-tocopherol. In vitro rumen dry matter and organic matter degradability, pH, ammonia, total volatile fatty acid, and gas production linearly decreased (p < 0.01), while neutral detergent fiber concentration linearly increased (p < 0.001), with increasing proportion of MS. The in vitro dry matter and organic matter degradability rapidly decreased when the MS percentage was ≥60%. In conclusion, the M40 silage is the most suitable for livestock utilization in local forage production considering the balance of silage pH, nutritional quality, and in vitro ruminal fermentation characteristics.

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