Effect of adding lignosulfonate and heat to canola screenings on ruminal and intestinal disappearance of dry matter and crude protein

2000 ◽  
Vol 80 (1) ◽  
pp. 215-219 ◽  
Author(s):  
Marina A. G. von Keyserlingk ◽  
E. Weurding ◽  
Mary Lou Swift ◽  
C. F. Wright ◽  
J. A. Shelford ◽  
...  
Keyword(s):  
Heat Treatment ◽  
Gastrointestinal Tract ◽  
Key Words ◽  
Crude Protein ◽  
Dry Matter ◽  
Holstein Cows ◽  
Lower Gastrointestinal Tract ◽  
Rumen Degradation ◽  
Heat Treated

The effect of adding 5% calcium lignosulfonate (LSO3) and heat to canola screenings on in situ ruminal and intestinal dry matter (DM) and crude protein (CP) disappearance in Holstein cows was investigated. Treatments consisted of untreated canola screenings, canola screenings that had been heated to 100 °C for 30 or 60 min and canola screenings that had 5% (wt wt−1) LSO3 added and were subsequently heated at 100 °C for 0, 30 or 60 min. Results indicate that treatment of canola screenings with 5% LSO3 and heated to 100 °C for 60 min was effective in reducing rumen degradation of DM and CP with a corresponding increase in disappearance in the lower gastrointestinal tract when compared to untreated and heat-treated canola screenings. Key words: Canola screenings, rumen by-pass protein, heat treatment, lignosulfonate

Effect of heat treatment and heat treatment in combination with lignosulfonate on in situ rumen degradability of canola cake crude protein, lysine, and methionine

2020 ◽  
Vol 100 (1) ◽  
pp. 165-174 ◽  
Author(s):  
Piotr Micek ◽  
Katarzyna Słota ◽  
Paweł Górka
Keyword(s):  
Heat Treatment ◽  
High Temperature ◽  
Crude Protein ◽  
Dry Matter ◽  
Increased Temperature ◽  
Heat Treated ◽  
Cold Pressed ◽  
Temperature Heating ◽  
High Temperature Heating

The aim of this study was to determine the effect of heat treatment alone or in combination with the addition of lignosulfonate (LSO3) on canola cake protein, lysine, and methionine degradation in the rumen. Cold-pressed canola cake was left untreated, heated at 90, 110, 130, or 150 °C, or processed with 5% of LSO3 (in dry matter) and then heated. Effective rumen degradability of crude protein (CP), lysine, and methionine was less for treated than untreated canola cake (P < 0.05) and decreased with increased temperature of heating, but particularly when canola cake was heated at 150 °C (quadratic, P < 0.01). In general, effective rumen degradability of CP, lysine, and methionine was less for canola cake heated at 130 °C in combination with LSO3 compared with canola cake heat treated only (quadratic × LSO3 interaction, P ≤ 0.07). Results of this study indicate that high temperature heating (130 °C or greater for 60 min) may be necessary to protect canola cake protein from degradation in the rumen, and the combination of heat treatment and LSO3 may be more effective in protecting canola cake protein, lysine, and methionine from degradation in the rumen than the use of heat treatment only.

scholarly journals A MODIFIED METHOD FOR DETERMINATION OF IN SITU RUMEN DEGRADATION OF FEEDSTUFFS

1987 ◽  
Vol 67 (1) ◽  
pp. 93-102 ◽  
Author(s):  
G. DE BOER ◽  
J. J. MURPHY ◽  
J. J. KENNELLY
Keyword(s):  
Crude Protein ◽  
Dry Matter ◽  
Holstein Cows ◽  
Polyester Mesh ◽  
Rumen Degradation ◽  
Modified Method ◽  
Constant Rate ◽  
Mesh Bag

In situ rumen dry matter (DM) and crude protein (CP) disappearance of various feed-stuffs was studied in Holstein cows. Comparisons of nylon bag size, method of containment within the rumen, and washing technique were made to develop a system which would reduce labor and improve precision among and within laboratories. Bags (7.0 × 11.0 cm) were suspended individually in the rumen by fishing line, or placed in a polyester mesh bag before being put in the rumen. No differences were observed between method of suspension in the rumen. The polyester mesh bag was then used to contain two nylon bag types (3.5 × 5.5 cm, 1-g sample (BT1); 7.0 × 11.0 cm; 5-g sample (BT2)) for incubation in the rumen. Small bags (BT1) were heat-sealed. Dry matter and CP disappearances from BT1 were lower than from BT2. These differences were attributed to washing technique as the larger bag (BT2) was washed more vigorously. A mechanical washing wheel was designed and built to reduce variability due to washing by hand. Bags washed by machine followed by hand washing had slightly higher DM and CP disappearances than bags washed by machine only; however, these differences did not significantly influence effective degradability. Feedstuffs were reincubated in small bags and large bags, and bags were mechanically washed. Dry matter and CP disappearance and effective rumen degradabilities of DM- and CP, assuming a constant rate of outflow, were not significantly different between bag types. Use of heat-sealed small bags (3.5 × 5.5 cm), containing 1-g samples incubated in the rumen within a polyester mesh bag, and a mechanical washer, provides a system for measuring in situ DM and CP disappearance from nylon bags which reduces labor input, can potentially improve consistency among and within laboratories, and facilitates high sample throughput. Key words: In situ, rumen, degradability, mechanical washer, Holstein cows

scholarly journals Effect of extruding on ruminal disappearance and lower gastrointestinal tract digestion of white lupin seeds

1993 ◽  
Vol 73 (3) ◽  
pp. 571-579 ◽  
Author(s):  
A. R. Kibelolaud ◽  
M. Vernay ◽  
C. Bayourthe ◽  
R. Moncoulon
Keyword(s):  
Gastrointestinal Tract ◽  
Crude Protein ◽  
Lupinus Albus ◽  
Neutral Detergent Fiber ◽  
White Lupin ◽  
Lower Gastrointestinal Tract ◽  
Rumen Degradation ◽  
Outflow Rate ◽  
Lupin Seeds

The effect of extruding white lupin (Lupinus albus 'Lublanc') seeds (WLS) at 110, 130, 150 or 180 °C on the in vitro solubility of crude protein (CP) and in sacco rumen degradation and intestinal digestion of rumen escape CP and fiber was determined. Rumen degradation was estimated by incubating nylon bags in the rumen of cows for 2, 4, 8, 16, 24 and 48 h. Extruding WLS at 110, 130, 150 and 180 °C reduced the CP-solubility by 32.8, 47.7, 58.4 and 67.5%, respectively. The effective ruminal degradabilities of CP, acid detergent fiber (ADF) and neutral detergent fiber (NDF) were evaluated assuming a ruminal outflow rate of 0.06 h−1. Heating WLS at 110, 130, 150 and 180 °C decreased the ruminal degradability of CP value: 89.8, 79.9, 65.1, 61.8 vs. 93.4% (raw), respectively; the corresponding values for ADF and NDF were: 38.4, 35.3, 34.2, 27.6 vs. 43.6% (raw) and 37.7, 33.1, 32.2, 26.5 vs. 39.4% (raw). Postruminal digestion was estimated using a sequence of ruminal in situ incubation for 4, 8 and 16 h, in vitro incubation in an acid-pepsin bath for 3 h and a mobile nylon bag technique distal to the abomasum. Extrusion of WLS decreased the degradability of CP, ADF and NDF in the rumen with a corresponding increase in the amounts digested in the postruminal sections; the whole-tract digestibility was generally unchanged. Therefore, the processing shifted the digestion of these components from rumen to the lower gastrointestinal tract. Key words: White lupin seed, in vitro solubility, rumen degradability, postruminal digestion, crude protein, detergent fiber

The rate and extent of silage nitrogen degradation in the rumen as influenced by wilting and duration of regrowth

1991 ◽  
Vol 71 (1) ◽  
pp. 245-248 ◽  
Author(s):  
N. F. Makoni ◽  
J. A. Shelford ◽  
L. J. Fisher
Keyword(s):  
Key Words ◽  
Crude Protein ◽  
Dry Matter ◽  
Hot Water ◽  
Protein Fractions ◽  
Rumen Degradation

The effect of duration of regrowth and wilting on silage crude protein fractions and rumen degradability of the crude protein were studied. The wilted silages had undergone more extensive proteolysis resulting in reduced hot water insoluble nitrogen. A large proportion of the silage crude protein was rumen degradable. The rates of degradation of the insoluble but degradable crude protein were not affected by wilting or the duration of regrowth. The results obtained indicate that wilting to only moderate dry matter appears to differ in its effect than wilting to higher DM. Key words: Silage, wilting, regrowth, nitrogen rumen, degradation

In situ rumen degradation of dry matter and crude protein in ewes and dairy cows

1999 ◽  
Vol 68 (4) ◽  
pp. 801-808 ◽  
Author(s):  
L. B. J. Šebek ◽  
H. Evererts
Keyword(s):  
Dairy Cows ◽  
Cold Exposure ◽  
Crude Protein ◽  
Dry Matter ◽  
Degradation Kinetics ◽  
Clear Trend ◽  
Rumen Degradation ◽  
Degradation Rates ◽  
Reference Protein

AbstractComparative studies were performed on the in-situ rumen degradation rate (kd) and the in-situ rumen undegradable residue (U) of dry matter (DM) and crude protein (CP) in sheep and dairy cows. The effect of different treatmentsfor sheep were investigated together with the validity of using cow-based reference protein values of foods for sheep. The effect of different breeds (Texel ewes and crossbred ewes), dietary roughage: concentrate ratio and shearing (mild cold exposure) were investigated for sheep.The in-situ rumen incubations were performed in accordance with standard operational procedures which were comparable for both species. The foods under consideration were hay and concentrates. Cows displayed lower (DM 0·53 , CP 0·86) Kds than sheep for concentrates.For hay a clear trend between cows and sheep was not observed but cows displayed 0·40 lower Kds than Texel ewes and 0·30 higher Kds than crossbred ewes. For concentrates U levels did not differ between species but for hay cows had 0·45 higher U values than Texel ewes. Texel ewes showed 0·45 higher Kd and 0·35 lower U for DM and CP than crossbred ewes. Dietary roughage: concentrate ratio in sheep diets did not influence the Kds and Us of the foods under consideration. Shearing of crossbred ewes increased Kd of CP by 0·25 and reduced U for DM and CP by 0·20 and 0.24 .In conclusion different in situ rumen degradation rates were found between dairy cows and sheep. This probably makes using reference protein values of foods invalid for sheep since they are based on data for cows. Differences (Kd andU) were also found between sheep breeds and between unshorn and shorn crossbred ewes (mild cold exposure). No effect on rumen degradation kinetics could be attributed to dietary roughage: concentrate ratio.

scholarly journals Evaluation of the Nutrient Composition, In Vitro Fermentation Characteristics, and In Situ Degradability of Amaranthus caudatus, Amaranthus cruentus, and Amaranthus hypochondriacus in Cattle

Animals ◽  
2020 ◽  
Vol 11 (1) ◽  
pp. 18
Author(s):  
Kim Margarette C. Nogoy ◽  
Jia Yu ◽  
Young Gyu Song ◽  
Shida Li ◽  
Jong-Wook Chung ◽  
...  
Keyword(s):  
Crude Protein ◽  
Dry Matter ◽  
Gas Production ◽  
Nutrient Composition ◽  
Neutral Detergent Fiber ◽  
Amaranthus Cruentus ◽  
Amaranthus Caudatus ◽  
Amaranthus Hypochondriacus

The amaranth plants showed high potential feed value as forage for ruminants. An in-depth study of this plant, particularly in cattle, will help extend its utilization as an alternative protein and fiber feed source in cattle feeding. In this study, the nutrient compositions of three different species of amaranth, Amaranthus caudatus L., Amaranthus cruentus L., and Amaranthus hypochondriacus L.—two varieties for each species, A.ca 74, A.ca 91, A.cu 62, A.cu 66, A. hy 30, and A. hy 48—were evaluated. The in vitro technique was used to evaluate the fermentation characteristics such as total gas production, total volatile fatty acids (VFA) concentration, pH, and ammonia concentration of the rumen fluid. Moreover, the effective degradabilities of dry matter (EDDM) and crude protein (EDCP) of the amaranth forages were determined through in situ bag technique. The amaranth forages: A. caudatus, A. cruentus, and A. hypochondriacus showed better nutritive value than the locally produced forages in Chungcheong province of Korea. The CP of the amaranth ranged from 11.95% to 14.19%, and the neutral detergent fiber (NDF) and acid detergent fiber (ADF) contents ranged from 45.53% to 70.88% and 34.17% to 49.83%, respectively. Among the amaranth varieties, A. hypochondriacus 48 showed the most excellent ruminant feed nutrient quality (CP, 14.19%; NDF, 45.53%; and ADF, 34.17%). The effective degradabilities of dry matter (EDDM; 33–56%) and crude protein EDCP (27–59%) of the amaranth were lower compared to other studies, which could be due to the maturity stage at which the forages were harvested. Nonetheless, A. hypochondriacus 48 showed the highest EDDM (56.73%) and EDCP (59.09%). The different amaranth species did not differ greatly in terms of total VFA concentration or molar proportions, total gas production, or ammonia-N concentration. The high nutrient composition, and highly effective degradability of dry matter and crude protein, coupled with the favorable fermentation characteristics, suggest that the amaranth forages showed good to excellent feed quality for cattle.

NUTRITIONAL VALUE OF HEAT TREATED WHOLE SOYBEANS

1971 ◽  
Vol 51 (1) ◽  
pp. 57-65 ◽  
Author(s):  
J. B. ARNOLD ◽  
J. D. SUMMERS ◽  
W. K. BILANSKI
Keyword(s):  
Heat Treatment ◽  
Crude Protein ◽  
Initial Moisture Content ◽  
Metabolizable Energy ◽  
Process Time ◽  
Commercial Diet ◽  
Heat Treated ◽  
Toxic Factors ◽  
Experimental Treatments ◽  
Quantity Of Heat

The heat treatment of whole soybeans was investigated, with consideration for oven temperature, process time and the initial moisture content of the beans, with a view to optimizing the removal of the toxic factors and enhancing the feed utilization of the soybean. Two purified diets equal in both crude protein level and classical metabolizable energy level were used. One of the diets consisted of ground whole soybeans (both heat treated and raw), whereas the other diet, containing commercial soybean meal as the only source of protein, provided the control for the tests. Male, White Leghorn cockerel chicks were selected on a weight basis and randomized in battery pens. The experimental treatments were assigned at random to pens and the chicks were provided feed and water ad libitum. Three experiments were conducted using soybeans with initial moisture of 10, 12.5 and 16% and heat treatment for 10, 5 and 3 minutes in a still-air oven within a temperature range of 149–246 C. Certain combinations of time and temperature not only promoted weight gains and feed efficiencies superior to those of the commercial diet, but also resulted in minimal pancreatic hypertrophy. The data obtained reaffirmed that a critical quantity of heat is required to deactivate the toxic factors and to ensure maximum nutritive availability.

Comparison of supplemental cobalt form on fibre digestion and cobalamin concentrations in cattle

2017 ◽  
Vol 155 (5) ◽  
pp. 832-838 ◽  
Author(s):  
R. C. WATERMAN ◽  
W. L. KELLY ◽  
C. K. LARSON ◽  
M. K. PETERSEN
Keyword(s):  
Crude Protein ◽  
Dry Matter ◽  
National Research Council ◽  
Residual Effect ◽  
Vitamin B ◽  
Serum Concentrations ◽  
Forage Digestibility ◽  
Randomized Experimental Design ◽  
To Receive

SUMMARYCobalt (Co) is essential for rumen microbial metabolism to synthesize methane, acetate and methionine. It also serves as a structural component of vitamin B12(cobalamin), which functions as a coenzyme in energy metabolism. A study was conducted to determine if Co form (carbonatev. glucoheptonate) supplemented above the National Research Council requirements would improve digestibility of a low-quality forage diet and change serum cobalamin concentrations. Nineteen ruminally cannulated cows (577 ± 13 kg) were fed individually in a completely randomized experimental design. Cows were fed a grass hay diet that contained (79·2 g/kg crude protein, 565 g/kg total digestible nutrients, 633·2 g/kg neutral detergent fibre (NDF), 874·2 g/kg dry matter) at a rate of 0·02% of body weight on a as fed basis for a 62-day study, which consisted of three periods; acclimation (AC), treatment (TR) and residual (RE). Measurements taken in the AC period were used as covariates for analysis in the TR and RE periods. Cows were stratified by age (5 ± 0·4 years) and lactational history, and assigned to receive 12·5 mg supplemental Co in one of two forms: (1) 27·2 mg of Co carbonate (CC,n= 11 cows) or (2) 50 mg of Co glucoheptonate (CGH,n= 8 cows). Supplement was administered daily via a gelatin capsule placed directly into the rumen 2 h after feeding. During the last 96 h of each period, forage digestibility was measured using anin situnylon bag technique. Blood samples were collected 4 and 6 h following feeding, and 24 h before the end of each period. A treatment × period interaction was detected forin situorganic matter (OM) disappearance at 96 h; (TR period: 684 and 708 ± 81 g/kg; RE period: 676 and 668 ± 75 g/kg, for CC and CGH, respectively). Once inclusion of Co in the CGH group was removed, OM disappearance was reduced by 4·01% compared with 0·82% in the CC cows. The NDF disappearance (OM basis) was less for the TR compared with the RE at 48 h (629 and 652 ± 39 g/kg, respectively). However, by 96 h NDF disappearance was greater for TR than the RE (704 and 689 ± 44 g/kg; respectively). No differences were detected for cobalamin serum concentrations or rate of fibre fermentation. The outcomes of the current research signify that there may be a slight residual effect of Co supplementation on fermentation; there was also an indication that Co source may enhance the overall extent of fermentation.

scholarly journals Nutritive value of sugarcane silages with different bacterial additives and fermentation periods

2020 ◽  
Vol 21 ◽  
Author(s):  
Ivone Rodrigues da Silva ◽  
Francirose Shigaki ◽  
Rosane Cláudia Rodrigues ◽  
Ana Paula Ribeiro Jesus ◽  
Clésio dos Santos Costa ◽  
...  
Keyword(s):  
Crude Protein ◽  
Dry Matter ◽  
Nutritional Value ◽  
Nutritive Value ◽  
Soluble Fraction ◽  
B Value ◽  
Neutral Detergent Fiber ◽  
Acid Detergent Fiber ◽  
Fermentation Period

ABSTRACT The objective of this study was to evaluate the nutritive value of sugarcane silage with or without inoculation with P. acidipropionici or L. buchneri, over three fermentation periods. The experimental design was completely randomized in a 3 x 3 inoculant by fermentation period factorial arrangement (without inoculant, inoculant 1, inoculant 2; x three fermentation periods, 10, 60, 90 days). Values of pH, dry matter (DM), crude protein (CP), neutral detergent fiber (NDF), acid detergent fiber (ADF), hemicellulose (HEM) and lignin were determined and in situ DM degradability profiles were modelled for parameters a, b and c, potential degradation (A) and effective degradability (ED). The 90 day fermentation yielded a lower pH for both inoculants. There was an interaction between inoculant and fermentation period (P < 0.05) for DM content, with a reduction in silage DM without the additive at 90 days. The CP, HEM, ADF and lignin contents of sugarcane were not influenced by the treatments. The addition of P. acidipropionici provided the lowest NDF content at 10 days and presented a higher fraction a, potential degradation and ED. At 60 days, there was no variation in soluble fraction, the control silage showed a higher fraction b, higher potential degradation and ED. At 90 days of fermentation, L. buchneri silages presented a higher fraction a, degradation rate and DE and a higher b value was obtained in the silage without inoculant. Inoculants are effective in maintaining the silage DM content and nutritional value during prolonged fermentation periods.

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