A MODIFIED METHOD FOR DETERMINATION OF IN SITU RUMEN DEGRADATION OF FEEDSTUFFS

In situ rumen dry matter (DM) and crude protein (CP) disappearance of various feed-stuffs was studied in Holstein cows. Comparisons of nylon bag size, method of containment within the rumen, and washing technique were made to develop a system which would reduce labor and improve precision among and within laboratories. Bags (7.0 × 11.0 cm) were suspended individually in the rumen by fishing line, or placed in a polyester mesh bag before being put in the rumen. No differences were observed between method of suspension in the rumen. The polyester mesh bag was then used to contain two nylon bag types (3.5 × 5.5 cm, 1-g sample (BT1); 7.0 × 11.0 cm; 5-g sample (BT2)) for incubation in the rumen. Small bags (BT1) were heat-sealed. Dry matter and CP disappearances from BT1 were lower than from BT2. These differences were attributed to washing technique as the larger bag (BT2) was washed more vigorously. A mechanical washing wheel was designed and built to reduce variability due to washing by hand. Bags washed by machine followed by hand washing had slightly higher DM and CP disappearances than bags washed by machine only; however, these differences did not significantly influence effective degradability. Feedstuffs were reincubated in small bags and large bags, and bags were mechanically washed. Dry matter and CP disappearance and effective rumen degradabilities of DM- and CP, assuming a constant rate of outflow, were not significantly different between bag types. Use of heat-sealed small bags (3.5 × 5.5 cm), containing 1-g samples incubated in the rumen within a polyester mesh bag, and a mechanical washer, provides a system for measuring in situ DM and CP disappearance from nylon bags which reduces labor input, can potentially improve consistency among and within laboratories, and facilitates high sample throughput. Key words: In situ, rumen, degradability, mechanical washer, Holstein cows

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Effect of adding lignosulfonate and heat to canola screenings on ruminal and intestinal disappearance of dry matter and crude protein

Canadian Journal of Animal Science ◽

10.4141/a99-071 ◽

2000 ◽

Vol 80 (1) ◽

pp. 215-219 ◽

Cited By ~ 6

Author(s):

Marina A. G. von Keyserlingk ◽

E. Weurding ◽

Mary Lou Swift ◽

C. F. Wright ◽

J. A. Shelford ◽

...

Keyword(s):

Heat Treatment ◽

Gastrointestinal Tract ◽

Key Words ◽

Crude Protein ◽

Dry Matter ◽

Holstein Cows ◽

Lower Gastrointestinal Tract ◽

Rumen Degradation ◽

Heat Treated

The effect of adding 5% calcium lignosulfonate (LSO3) and heat to canola screenings on in situ ruminal and intestinal dry matter (DM) and crude protein (CP) disappearance in Holstein cows was investigated. Treatments consisted of untreated canola screenings, canola screenings that had been heated to 100 °C for 30 or 60 min and canola screenings that had 5% (wt wt−1) LSO3 added and were subsequently heated at 100 °C for 0, 30 or 60 min. Results indicate that treatment of canola screenings with 5% LSO3 and heated to 100 °C for 60 min was effective in reducing rumen degradation of DM and CP with a corresponding increase in disappearance in the lower gastrointestinal tract when compared to untreated and heat-treated canola screenings. Key words: Canola screenings, rumen by-pass protein, heat treatment, lignosulfonate

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Determination of degradation in the rumen for dry matter, organic matter and crude protein of alfalfa (Medicago sativa L.) harvested at differentmaturity stages using the in situ nylon bag method

Indian Journal of Animal Research ◽

10.18805/ijar.11177 ◽

2016 ◽

Author(s):

Elif Karayilanli ◽

Veysel Ayhan

Keyword(s):

Organic Matter ◽

Medicago Sativa ◽

Crude Protein ◽

Dry Matter ◽

Seed Setting ◽

Maturity Stages ◽

Mature Period ◽

Medicago Sativa L

The aim of this study was to determine the degradation in the rumen for the dry matter (DM), organic matter (OM) and crude protein (CP) of alfalfa (Medicago sativa L.) harvested at different maturity stages by using an in situ nylon bag method. Maturity and harvesting effects were observed in ruminally degradable DM, OM and CP. High correlations between in situ degradability and maturity were determined. Alfalfa harvested in the bud period exhibited more extensive ruminal DM, OM and CP degradation than that harvested at other maturity stages. The alfalfa hay’s DM, OM and CP degradability were lowest (62.18%, 59.52% and 76.31%, respectively) in its most mature period of seed setting; its degradability decreased with maturity (17%, 14% and 13%, respectively).

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Digestión de materia seca, proteína cruda y aminoácidos de la dieta de vacas lecheras

Agronomía Mesoamericana ◽

10.15517/ma.v28i2.25643 ◽

2017 ◽

Vol 28 (2) ◽

pp. 341

Author(s):

Monica Duque Quintero ◽

Ricardo Rosero Noguera ◽

Marta Olivera Ángel

Keyword(s):

Amino Acids ◽

Dairy Cows ◽

Crude Protein ◽

Dry Matter ◽

Descriptive Statistics ◽

Passage Rate ◽

Ruminal Degradability ◽

Kikuyu Grass

The aim of this study was to determine the digestion of dry matter (MS), crude protein (PC) and amino acids (AA) in dairy cows. Two cannulated cows were used for the determination of passage rate (Kp), in situ ruminal degradability (DR) and intestinal digestibility (DI) by abomasal catheter. The data to calculate Kp was analyzed with NLIN procedure by SAS, and descriptive statistics for DR and DI of MS, PC and AA from Kikuyu grass (Pennisetum clandestinum), a commercial supplement and two sources of rumen-protected AA. The study showed a Kp and a ruminal retention time of 0.036 h-1 and 27.4 h. The highest values from DR of MS and CP were from Kikuyu grass (69.0 and 61.8%) and concentrate (84.7 and 77.2%), followed by MetP (60.2 and 66.7%) and LysP (6.72 and 11.4%). The highest percentages of rumen indegradable amino acids (AADR) were from Kikuyu and concentrate, varying between 58.7 and 68% in forage, and 76.1 and 82.9% in the concentrate. The DR was 11.5 and 65.8% in LysP and MetP, respectively. The DI of AA (%AADR) varied between 42.3 and 77.4% for Kikuyu and 42.2 and 59.3% for concentrate. The values for the protected amino acids were 42.1 for LysP and 58.6 for MetP.

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In situ rumen degradation of dry matter and crude protein in ewes and dairy cows

Animal Science ◽

10.1017/s1357729800050827 ◽

1999 ◽

Vol 68 (4) ◽

pp. 801-808 ◽

Cited By ~ 8

Author(s):

L. B. J. Šebek ◽

H. Evererts

Keyword(s):

Dairy Cows ◽

Cold Exposure ◽

Crude Protein ◽

Dry Matter ◽

Degradation Kinetics ◽

Clear Trend ◽

Rumen Degradation ◽

Degradation Rates ◽

Reference Protein

AbstractComparative studies were performed on the in-situ rumen degradation rate (kd) and the in-situ rumen undegradable residue (U) of dry matter (DM) and crude protein (CP) in sheep and dairy cows. The effect of different treatmentsfor sheep were investigated together with the validity of using cow-based reference protein values of foods for sheep. The effect of different breeds (Texel ewes and crossbred ewes), dietary roughage: concentrate ratio and shearing (mild cold exposure) were investigated for sheep.The in-situ rumen incubations were performed in accordance with standard operational procedures which were comparable for both species. The foods under consideration were hay and concentrates. Cows displayed lower (DM 0·53 , CP 0·86) Kds than sheep for concentrates.For hay a clear trend between cows and sheep was not observed but cows displayed 0·40 lower Kds than Texel ewes and 0·30 higher Kds than crossbred ewes. For concentrates U levels did not differ between species but for hay cows had 0·45 higher U values than Texel ewes. Texel ewes showed 0·45 higher Kd and 0·35 lower U for DM and CP than crossbred ewes. Dietary roughage: concentrate ratio in sheep diets did not influence the Kds and Us of the foods under consideration. Shearing of crossbred ewes increased Kd of CP by 0·25 and reduced U for DM and CP by 0·20 and 0.24 .In conclusion different in situ rumen degradation rates were found between dairy cows and sheep. This probably makes using reference protein values of foods invalid for sheep since they are based on data for cows. Differences (Kd andU) were also found between sheep breeds and between unshorn and shorn crossbred ewes (mild cold exposure). No effect on rumen degradation kinetics could be attributed to dietary roughage: concentrate ratio.

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Evaluation of the Nutrient Composition, In Vitro Fermentation Characteristics, and In Situ Degradability of Amaranthus caudatus, Amaranthus cruentus, and Amaranthus hypochondriacus in Cattle

Animals ◽

10.3390/ani11010018 ◽

2020 ◽

Vol 11 (1) ◽

pp. 18

Author(s):

Kim Margarette C. Nogoy ◽

Jia Yu ◽

Young Gyu Song ◽

Shida Li ◽

Jong-Wook Chung ◽

...

Keyword(s):

Crude Protein ◽

Dry Matter ◽

Gas Production ◽

Nutrient Composition ◽

Neutral Detergent Fiber ◽

Amaranthus Cruentus ◽

Amaranthus Caudatus ◽

Amaranthus Hypochondriacus

The amaranth plants showed high potential feed value as forage for ruminants. An in-depth study of this plant, particularly in cattle, will help extend its utilization as an alternative protein and fiber feed source in cattle feeding. In this study, the nutrient compositions of three different species of amaranth, Amaranthus caudatus L., Amaranthus cruentus L., and Amaranthus hypochondriacus L.—two varieties for each species, A.ca 74, A.ca 91, A.cu 62, A.cu 66, A. hy 30, and A. hy 48—were evaluated. The in vitro technique was used to evaluate the fermentation characteristics such as total gas production, total volatile fatty acids (VFA) concentration, pH, and ammonia concentration of the rumen fluid. Moreover, the effective degradabilities of dry matter (EDDM) and crude protein (EDCP) of the amaranth forages were determined through in situ bag technique. The amaranth forages: A. caudatus, A. cruentus, and A. hypochondriacus showed better nutritive value than the locally produced forages in Chungcheong province of Korea. The CP of the amaranth ranged from 11.95% to 14.19%, and the neutral detergent fiber (NDF) and acid detergent fiber (ADF) contents ranged from 45.53% to 70.88% and 34.17% to 49.83%, respectively. Among the amaranth varieties, A. hypochondriacus 48 showed the most excellent ruminant feed nutrient quality (CP, 14.19%; NDF, 45.53%; and ADF, 34.17%). The effective degradabilities of dry matter (EDDM; 33–56%) and crude protein EDCP (27–59%) of the amaranth were lower compared to other studies, which could be due to the maturity stage at which the forages were harvested. Nonetheless, A. hypochondriacus 48 showed the highest EDDM (56.73%) and EDCP (59.09%). The different amaranth species did not differ greatly in terms of total VFA concentration or molar proportions, total gas production, or ammonia-N concentration. The high nutrient composition, and highly effective degradability of dry matter and crude protein, coupled with the favorable fermentation characteristics, suggest that the amaranth forages showed good to excellent feed quality for cattle.

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In vitro dry matter and crude protein rumen degradation and abomasal digestibility of soybean meal treated with chestnut and quebracho wood extracts

Food Science & Nutrition ◽

10.1002/fsn3.2072 ◽

2020 ◽

Author(s):

Andrej Lavrenčič ◽

Alenka Levart

Keyword(s):

Soybean Meal ◽

Crude Protein ◽

Dry Matter ◽

Rumen Degradation ◽

Wood Extracts

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Comparison of supplemental cobalt form on fibre digestion and cobalamin concentrations in cattle

The Journal of Agricultural Science ◽

10.1017/s0021859617000107 ◽

2017 ◽

Vol 155 (5) ◽

pp. 832-838 ◽

Cited By ~ 1

Author(s):

R. C. WATERMAN ◽

W. L. KELLY ◽

C. K. LARSON ◽

M. K. PETERSEN

Keyword(s):

Crude Protein ◽

Dry Matter ◽

National Research Council ◽

Residual Effect ◽

Vitamin B ◽

Serum Concentrations ◽

Forage Digestibility ◽

Randomized Experimental Design ◽

To Receive

SUMMARYCobalt (Co) is essential for rumen microbial metabolism to synthesize methane, acetate and methionine. It also serves as a structural component of vitamin B12(cobalamin), which functions as a coenzyme in energy metabolism. A study was conducted to determine if Co form (carbonatev. glucoheptonate) supplemented above the National Research Council requirements would improve digestibility of a low-quality forage diet and change serum cobalamin concentrations. Nineteen ruminally cannulated cows (577 ± 13 kg) were fed individually in a completely randomized experimental design. Cows were fed a grass hay diet that contained (79·2 g/kg crude protein, 565 g/kg total digestible nutrients, 633·2 g/kg neutral detergent fibre (NDF), 874·2 g/kg dry matter) at a rate of 0·02% of body weight on a as fed basis for a 62-day study, which consisted of three periods; acclimation (AC), treatment (TR) and residual (RE). Measurements taken in the AC period were used as covariates for analysis in the TR and RE periods. Cows were stratified by age (5 ± 0·4 years) and lactational history, and assigned to receive 12·5 mg supplemental Co in one of two forms: (1) 27·2 mg of Co carbonate (CC,n= 11 cows) or (2) 50 mg of Co glucoheptonate (CGH,n= 8 cows). Supplement was administered daily via a gelatin capsule placed directly into the rumen 2 h after feeding. During the last 96 h of each period, forage digestibility was measured using anin situnylon bag technique. Blood samples were collected 4 and 6 h following feeding, and 24 h before the end of each period. A treatment × period interaction was detected forin situorganic matter (OM) disappearance at 96 h; (TR period: 684 and 708 ± 81 g/kg; RE period: 676 and 668 ± 75 g/kg, for CC and CGH, respectively). Once inclusion of Co in the CGH group was removed, OM disappearance was reduced by 4·01% compared with 0·82% in the CC cows. The NDF disappearance (OM basis) was less for the TR compared with the RE at 48 h (629 and 652 ± 39 g/kg, respectively). However, by 96 h NDF disappearance was greater for TR than the RE (704 and 689 ± 44 g/kg; respectively). No differences were detected for cobalamin serum concentrations or rate of fibre fermentation. The outcomes of the current research signify that there may be a slight residual effect of Co supplementation on fermentation; there was also an indication that Co source may enhance the overall extent of fermentation.

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Nutritive value of sugarcane silages with different bacterial additives and fermentation periods

Revista Brasileira de Saúde e Produção Animal ◽

10.1590/s1519-9940210212020 ◽

2020 ◽

Vol 21 ◽

Author(s):

Ivone Rodrigues da Silva ◽

Francirose Shigaki ◽

Rosane Cláudia Rodrigues ◽

Ana Paula Ribeiro Jesus ◽

Clésio dos Santos Costa ◽

...

Keyword(s):

Crude Protein ◽

Dry Matter ◽

Nutritional Value ◽

Nutritive Value ◽

Soluble Fraction ◽

B Value ◽

Neutral Detergent Fiber ◽

Acid Detergent Fiber ◽

Fermentation Period

ABSTRACT The objective of this study was to evaluate the nutritive value of sugarcane silage with or without inoculation with P. acidipropionici or L. buchneri, over three fermentation periods. The experimental design was completely randomized in a 3 x 3 inoculant by fermentation period factorial arrangement (without inoculant, inoculant 1, inoculant 2; x three fermentation periods, 10, 60, 90 days). Values of pH, dry matter (DM), crude protein (CP), neutral detergent fiber (NDF), acid detergent fiber (ADF), hemicellulose (HEM) and lignin were determined and in situ DM degradability profiles were modelled for parameters a, b and c, potential degradation (A) and effective degradability (ED). The 90 day fermentation yielded a lower pH for both inoculants. There was an interaction between inoculant and fermentation period (P < 0.05) for DM content, with a reduction in silage DM without the additive at 90 days. The CP, HEM, ADF and lignin contents of sugarcane were not influenced by the treatments. The addition of P. acidipropionici provided the lowest NDF content at 10 days and presented a higher fraction a, potential degradation and ED. At 60 days, there was no variation in soluble fraction, the control silage showed a higher fraction b, higher potential degradation and ED. At 90 days of fermentation, L. buchneri silages presented a higher fraction a, degradation rate and DE and a higher b value was obtained in the silage without inoculant. Inoculants are effective in maintaining the silage DM content and nutritional value during prolonged fermentation periods.

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