scholarly journals Differential regulation of components of the apoptotic and ubiquitin-mediated proteolytic pathway in slow- and fast- twitch muscle type in lambs receiving increasing amounts of dietary non-structural carbohydrate

2010 ◽  
Vol 90 (3) ◽  
pp. 421-428 ◽  
Author(s):  
S L Greenwood ◽  
M. Steele ◽  
O. AlZahal ◽  
S E Hook ◽  
K C Swanson ◽  
...  
Keyword(s):  
Mrna Expression ◽  
Dry Matter ◽  
Control Diet ◽  
Extracellular Fluid ◽  
Total Carbon ◽  
Α Subunit ◽  
Messenger Ribonucleic Acid ◽  
Proteolytic Pathway ◽  
Fast Twitch Muscle ◽  
Fast Twitch

Non-fibre carbohydrate (NFC) rich diets are commonly fed in ruminant production systems. The objective was to determine whether NFC challenge affects proteasome activity, or messenger ribonucleic acid (mRNA) expression of proteasome subunits or apoptotic Bcl-2 proteins, in slow- or fast- twitch muscle of sheep. For 12 d prior to slaughter, lambs (n = 8) received either a control diet (28.4% of dry matter as grain), or a diet of increasing amounts of grain up to 79.1% of dry matter. A decrease in urinary pH (P = 0.01), base excess of blood and extracellular fluid (P = 0.01), bicarbonate (P = 0.03) and total carbon dioxide (P = 0.04), and an increase in anion gap (P = 0.07) in NFC lambs are indicative of metabolic acidosis. NFC lambs had significantly lower mRNA expression of the 20S -β subunit (P = 0.05), and a tendency toward lower mRNA expression of the 20S-α subunit (P = 0.11) and the 19S isoform (P = 0.15) in soleus, but not to the same extent in extensor digitorum longus (EDL) muscle. Downregulation of Bad mRNA expression occurred in both soleus (P = 0.10) and EDL (P = 0.08) muscle as a result of NFC challenge. These results indicate that NFC challenge does affect mRNA expression of genes related to the proteasome and apoptosis in a muscle specific manner. Key words: Ubiquitin-mediated proteolytic pathway, non-fibre carbohydrate, apoptosis, sheep

ACCEPTABILITY AND UTILIZATION OF GRADED LEVELS OF GMELINA ARBOREA AND CASSAVA PEELS CONCENTRATES IN WEST AFRICAN DWARF SHEEP

2015 ◽  
Vol 4 (2) ◽  
pp. 415-422
Author(s):  
Amoka Pius ◽  
Tawose O M
Keyword(s):  
Dry Matter ◽  
Nutritive Value ◽  
Control Diet ◽  
Nutrient Digestibility ◽  
Dry Matter Intake ◽  
Gmelina Arborea ◽  
Leaf Meal ◽  
Dry Matter Digestibility ◽  
Inclusion Level ◽  
Cassava Peels

The nutritive value ,voluntary dry matter intake, and the nutrient digestibility of graded levels of Gmelina arborea and cassava peels concentrates in WAD sheep was investigated. Twelve WAD sheep aged 1-2 years old and weighting 14.00± 0.45 kg were used in a complete randomized design. Diets were formulated such that cassava peels was replaced with Gmelina arborea leaf meal at 0, 33.33, 66.67, 100% levels, designated as diets A, B, C, and D respectively. Diet without Gmelina arborea leaf meal was tagged the control diet. The concentrate feed was compounded to contain 16% CP. Diets with 33.33% inclusion level of Gmelina arborea had significantly (P<0.05) higher dry matter intake (DMI) 598.80g day-1, while the lowest DMI 425.00g day-1 was obtained in animals fed 100% inclusion level of Gmelina arborea. Crude protein intake (CPI) of animals fed diets with 33.33% inclusion levels of Gmelina arborea were significantly (P<0.05) highest, followed by 66.67% inclusion level and the least was observed in 0% inclusion level of Gmelina arborea. Dry matter digestibility (DMD) was significantly (P<0.05) different across the dietary treatments, animals placed on diets with 33.33% inclusion level had the highest DMD, followed by animals on diets with 66.67, 100 and 0% inclusion levels. CP digestibility (P<0.05) increased from 33.33% to 100% inclusion levels of Gmelina arborea leaf meal, the lowest CP digestibility was observed at 0% inclusion level. CF digestibility (P<0.05) increased from 33.33% to 100% inclusion levels of Gmelina arborea leaf meal, while the lowest CF digestibility was observed at 0% inclusion level. N intake increased significantly (P<0.05) with increase in the level of Gmelina arborea inclusion from 33.33% to 100%. N retention was significantly (P<0.05) different, diets with 33.33% Gmelina arborea inclusion had the highest value (64.36g day-1) followed by 66.67%, 100% and the least (52.64g day-1) was at 0% inclusion level of Gmelina arborea.  N balance values also followed the same trend. From the results of this study, it can be concluded that the inclusion of Gmelina arborea leaf meal in WAD rams diet was well tolerated without adverse effect on acceptability, intake and nutrient digestibility, and inclusion level of 33.33% is hereby recommended in ruminants diet for optimum performance and productivity.

scholarly journals A Grain-Based SARA Challenge Affects the Composition of Epimural and Mucosa-Associated Bacterial Communities throughout the Digestive Tract of Dairy Cows

Animals ◽  
2021 ◽  
Vol 11 (6) ◽  
pp. 1658
Author(s):  
Jan C. Plaizier ◽  
Anne-Mette Danscher ◽  
Paula A. Azevedo ◽  
Hooman Derakhshani ◽  
Pia H. Andersen ◽  
...  
Keyword(s):  
Digestive Tract ◽  
Relative Abundance ◽  
Bacterial Communities ◽  
Dry Matter ◽  
Control Diet ◽  
Rrna Gene ◽  
Ruminal Acidosis ◽  
Clustering Patterns ◽  
The 16S Rrna Gene ◽  
Distal Jejunum

The effects of a subacute ruminal acidosis (SARA) challenge on the composition of epimural and mucosa-associated bacterial communities throughout the digestive tract were determined in eight non-lactating Holstein cows. Treatments included feeding a control diet containing 19.6% dry matter (DM) starch and a SARA-challenge diet containing 33.3% DM starch for two days after a 4-day grain step-up. Subsequently, epithelial samples from the rumen and mucosa samples from the duodenum, proximal, middle and distal jejunum, ileum, cecum and colon were collected. Extracted DNA from these samples were analyzed using MiSeq Illumina sequencing of the V4 region of the 16S rRNA gene. Distinct clustering patterns for each diet existed for all sites. The SARA challenge decreased microbial diversity at all sites, with the exception of the middle jejunum. The SARA challenge also affected the relative abundances of several major phyla and genera at all sites but the magnitude of these effects differed among sites. In the rumen and colon, the largest effects were an increase in the relative abundance of Firmicutes and a reduction of Bacteroidetes. In the small intestine, the largest effect was an increase in the relative abundance of Actinobacteria. The grain-based SARA challenge conducted in this study did not only affect the composition and cause dysbiosis of epimural microbiota in the rumen, it also affected the mucosa-associated microbiota in the intestines. To assess the extent of this dysbiosis, its effects on the functionality of these microbiota must be determined in future.

The impact of fibropapillomatosis on clinical characteristics, blood gas, plasma biochemistry, and hematological profiles in juvenile green turtles ( Chelonia mydas)

2020 ◽  
Vol 96 (4) ◽  
pp. 723-734
Author(s):  
Tsung-Hsien Li ◽  
Chao-Chin Chang
Keyword(s):  
White Blood Cells ◽  
Extracellular Fluid ◽  
Chelonia Mydas ◽  
Sea Turtle ◽  
Total Carbon ◽  
Blood Gas ◽  
Green Turtles ◽  
Cell Counts ◽  
Significant Difference ◽  
The Impact

Fibropapillomatosis (FP) is a tumor- forming disease that afflicts all marine turtles and is the most common in green turtles (Chelonia mydas). In this study, the morphometric characteristics, blood gas, biochemistry, and hematological profiles of 28 (6 FP-positive and 22 FP-negative) green turtles from the coast of Taiwan were investigated. The results indicated that body weight ( P < 0.001) and curved carapace length (CCL; P < 0.001) in green turtles with FP were significantly higher than in turtles without FP. Furthermore, green turtles with FP had a significantly lower value of hemoglobin (HB; P = 0.010) and packed cell volume (PCV; P = 0.005) than turtles without FP. Blood cell counts of white blood cells (WBC; P = 0.008) and lymphocytes ( P = 0.022) were observed with significant difference; green turtles with FP had lower counts than turtles without FP. In addition, turtles with FP had significantly higher pH ( P = 0.036), base excess in extracellular fluid (BEecf; P = 0.012), bicarbonate (HCO3– ; P = 0.008), and total carbon dioxide (TCO2 ; P = 0.025) values than turtles without FP. The findings of this study provide valuable clinical parameters for the medical care of the species in sea turtle rehabilitation centers and help us to understand the physiological response of green turtles to different tumor-forming conditions.

scholarly journals Adrenergic control of vascular resistance varies in muscles composed of different fiber types: influence of the vascular endothelium

2011 ◽  
Vol 301 (3) ◽  
pp. R783-R790 ◽  
Author(s):  
Bradley J. Behnke ◽  
Robert B. Armstrong ◽  
Michael D. Delp
Keyword(s):  
Blood Flow ◽  
Vascular Resistance ◽  
Vascular Endothelium ◽  
Fold Increase ◽  
Male Rats ◽  
Type I ◽  
Fiber Types ◽  
Fast Twitch Muscle ◽  
Dose Responses ◽  
Fast Twitch

The influence of the sympathetic nervous system (SNS) upon vascular resistance is more profound in muscles comprised predominately of low-oxidative type IIB vs. high-oxidative type I fiber types. However, within muscles containing high-oxidative type IIA and IIX fibers, the role of the SNS on vasomotor tone is not well established. The purpose of this study was to examine the influence of sympathetic neural vasoconstrictor tone in muscles composed of different fiber types. In adult male rats, blood flow to the red and white portions of the gastrocnemius (GastRed and GastWhite, respectively) and the soleus muscle was measured pre- and postdenervation. Resistance arterioles from these muscles were removed, and dose responses to α1-phenylephrine or α2-clonidine adrenoreceptor agonists were determined with and without the vascular endothelium. Denervation resulted in a 2.7-fold increase in blood flow to the soleus and GastRed and an 8.7-fold increase in flow to the GastWhite. In isolated arterioles, α2-mediated vasoconstriction was greatest in GastWhite (∼50%) and less in GastRed (∼31%) and soleus (∼17%); differences among arterioles were abolished with the removal of the endothelium. There was greater sensitivity to α1-mediated vasoconstriction in the GastWhite and GastRed vs. the soleus, which was independent of whether the endothelium was present. These data indicate that 1) control of vascular resistance by the SNS in high-oxidative, fast-twitch muscle is intermediate to that of low-oxidative, fast-twitch and high-oxidative, slow-twitch muscles; and 2) the ability of the SNS to control blood flow to low-oxidative type IIB muscle appears to be mediated through postsynaptic α1- and α2-adrenoreceptors on the vascular smooth muscle.

Annual ryegrass (Lolium rigidum) seed survival and digestibility in cattle and sheep

2002 ◽  
Vol 42 (2) ◽  
pp. 111 ◽  
Author(s):  
R. Stanton ◽  
J. Piltz ◽  
J. Pratley ◽  
A. Kaiser ◽  
D. Hudson ◽  
...  
Keyword(s):  
Dry Matter ◽  
Control Diet ◽  
Basal Diet ◽  
Annual Ryegrass ◽  
Lolium Rigidum ◽  
Seed Survival ◽  
Dry Matter Digestibility ◽  
Cattle And Sheep

A trial was conducted to investigate the survival and digestibility of annual ryegrass (ARG) seed (Lolium rigidum L.) eaten by sheep and cattle. Sheep (n= 8) and cattle (n = 8) were fed a basal diet containing 1:1 lucerne chaff:oaten chaff with (ARG) or without (control) the inclusion of 20% total dry matter of annual ryegrass seed in a changeover design. Intake was restricted to 17 g/kg liveweight. Digestibility of the control diet was lower (P<0.01) for sheep than cattle. Annual ryegrass seed was present (P<0.01) in the faeces of both sheep and cattle within 24 h of first ingestion. Some 10.8 and 32.8% of seed ingested was excreted by sheep and cattle respectively, with 3.9% (sheep) and 11.9% (cattle) remaining germinable. Annual ryegrass seed continued to be excreted by both sheep and cattle up to 5 days after removal from the diet. Dry matter digestibility of the annual ryegrass diet was 53% in cattle.

scholarly journals High-speed jaw-opening exercise in training suprahyoid fast-twitch muscle fibers

2018 ◽  
Vol Volume 13 ◽  
pp. 125-131 ◽  
Author(s):  
Mariko Matsubara ◽  
Haruka Tohara ◽  
Koji Hara ◽  
Hiromichi Shinozaki ◽  
Yasuhiro Yamazaki ◽  
...  
Keyword(s):  
High Speed ◽  
Muscle Fibers ◽  
Jaw Opening ◽  
Fast Twitch Muscle ◽  
Fast Twitch

Effect of pravastatin on cisplatin-induced nephrotoxicity in rats

2010 ◽  
Vol 30 (7) ◽  
pp. 603-615 ◽  
Author(s):  
Mikiya Fujieda ◽  
Taku Morita ◽  
Keishi Naruse ◽  
Yoshihiro Hayashi ◽  
Masayuki Ishihara ◽  
...  
Keyword(s):  
Mrna Expression ◽  
Serum Lipids ◽  
Control Diet ◽  
Lipid Lowering ◽  
Tunel Staining ◽  
Tubular Damage ◽  
P53 Expression ◽  
Group 2 ◽  
Expression And Activity ◽  
Group 1

We investigated whether pravastatin ameliorates renal damage induced by cisplatin (CP). Forty-three male Wistar rats were divided into four groups: rats treated with a control diet for 19 days and saline injection on day 14 (group1), group 1 with pravastatin treatment with 19 days (group 2), group 1 with CP injection on day 14 (group 3), and group 2 with CP injection (group 4). Renal function and serum lipids, renal malondialdehyde (MDA) and glutathione (GSH) levels, glutathione peroxidase (GPx) mRNA expression and activity, and kidney triglyceride (TG) concentrations were measured. Histology was evaluated by light microscopy with immunohistochemistry for p53, p53-upregulated modulation of apoptosis (PUMA), and terminal deoxynucleotide transferase dUTP nick end-labeling (TUNEL) staining. CP induced renal tubular damage with a higher MDA level, increased PUMA expression, p53- and TUNEL-positive cells counts, elevation of serum lipids, and decreased GSH level, GPx mRNA expression, and activity. Pravastatin partially ameliorated CP-induced renal injury, based on suppression of the renal MDA and TG levels, decreased p53 expression, and apoptosis in CP-treated rats. These findings suggest that pravastatin has a partial protective effect against CP nephrotoxicity via antioxidant activity as well as attenuation of the p53 response, and lipid-lowering effects.

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