EFFECTS OF DIFFERENT DRY MATTER DETERMINATION METHODS ON CHEMICAL COMPOSITION AND IN VITRO DIGESTIBILITY OF SILAGES

1973 ◽  
Vol 53 (4) ◽  
pp. 753-760 ◽  
Author(s):  
R. E. LARSEN ◽  
G. M. JONES
Keyword(s):  
Organic Matter ◽  
Crude Protein ◽  
Dry Matter ◽  
In Vitro Digestibility ◽  
Cellulose Content ◽  
Freeze Dried ◽  
Gross Energy ◽  
Determination Methods

Dry matter (DM) contents of 13 silages, comprised of 5 corn, 4 legume–grass, and 4 corn–hay crop silage mixtures, were determined by (1) toluene distillation (TD), corrected for volatiles lost into the aqueous distillate; (2) TD, uncorrected; (3) freeze-drying (FD); (4) oven-drying (OD) at 40 C; (5) OD at 65 C; and (6) moisture-tester at 100 C. Corrected TD showed significantly higher (P < 0.01) DM levels compared with other methods. Up to 12.8% loss of DM resulted from heat-drying, depending on the method and the sample. Dried-ground silages were analyzed for nutrient composition. No significant changes in cell-wall constituents or organic matter contents were observed. Silages that were FD were highest in cellulose content (P < 0.05), whereas both FD and OD at 40 C resulted in significant increases in gross energy (P < 0.05) and crude protein (P < 0.01) content compared with other methods. FD or OD at 40 C resulted in the highest dry matter solubility and in vitro digestibility of cellulose, dry matter, and organic matter (P < 0.05). The lowest values for these analyses were obtained with silages heat-dried at 65 and 100 C. Results of these studies suggest that corrected TD should be used for determination of silage DM content and that in vitro analyses or other biochemical determinations should be conducted on silage samples that have been freeze-dried or oven-dried at 40 C.

scholarly journals Sunflower cake in diets for beef cattle: digestibility, kinetics and in vitro ruminal fermentation parameters

2018 ◽  
Vol 41 (1) ◽  
pp. 39492 ◽  
Author(s):  
Rafael Henrique de Tonissi e Buschinelli de Goes ◽  
Luiz Henrique Xavier da Silva ◽  
Tatiana García Díaz ◽  
Antonio Ferriani Branco ◽  
Ana Lúcia Teodoro ◽  
...  
Keyword(s):  
Organic Matter ◽  
Beef Cattle ◽  
Soybean Meal ◽  
Crude Protein ◽  
Dry Matter ◽  
In Vitro Digestibility ◽  
Ruminal Fermentation ◽  
Fermentation Products ◽  
Sunflower Cake

 The objective of this study was to evaluate the effect of the inclusion of sunflower cake replacing soybean meal in beef cattle diets on the in vitro digestibility of dry matter (IVDDM), organic matter (IVDOM), crude protein (IVDCP) and the ruminal fermentation kinetics and parameters. The experiment was analyzed according to a completely randomized design. The treatments consisted of four levels of sunflower cake, 0, 200, 400, 600 g kg-1, replacing soybean meal in the concentrate of beef cattle diets. The coefficients of IVDDM, IVDOM and IVDCP presented a quadratic effect with the addition of sunflower cake. The soluble fraction (fraction B) degradation rate and total gas production decreased linearly with the inclusion of sunflower cake. Values of pH in ruminal fluid were higher for levels 0, 200 and 600 g kg-1 sunflower cake. Sunflower cake can replace soybean meal by up to 280 g kg-1 in the concentrate of beef cattle diets, improving the in vitro digestibility of dry matter organic, matter and crude protein. Levels above 400 g kg-1 reduce ruminal digestion rate, digestibility and release of final fermentation products. 

scholarly journals Chemical composition and in vitro digestibility of some range plants

2021 ◽  
pp. 094-098
Author(s):  
Musa Tibin Musa Ahmed
Keyword(s):  
Organic Matter ◽  
Chemical Composition ◽  
Protein Content ◽  
Crude Protein ◽  
Dry Matter ◽  
In Vitro Digestibility ◽  
Maturity Stage ◽  
Legume Species ◽  
Significant Difference

This study was carried out to determine chemical composition and in vitro dry matter and organic matter digestibility of range plant species of the area. Samples were collected from range lands at full maturity stage, dried under shade and subjected to chemical analysis and dry matter and organic matter in vitro digestibility for Vigna sunhum, Andropogan gayanus, Pennisetum Pedicelatum, Shoenfeldia gracilis and Stylothanis flavicans were determined. The data was analyzed via analysis of variance and differences among means were determined using Least Significant Difference (LSD) test. The results showed that the legumes (Vigna sunhum and Stylothanis flavicans) were significantly higher (P≤0.01) in their crude protein content and the percentage of crude protein respectively was 13.4% and 10.00% while the grasses were significant (P≤0.05) higher in Crude Fibre (CF), ash, Acid Detergent Fibers (ADF) and Neutral Detergent Fibers (NDF). Crude fiber % values were 41.78, 43.37 and 46.07% for Pennisetum Pedicelatum, Andropogan gayanus and Shoenofeldia gracilis, respectively. The acid detergent values were 53.85, 47.74 and 53.39 for the three weeds. (P≤0.05), while the ratio of neutral detergent was significantly higher (P≤.05) in grasses than in the two legume species; 87.22, 82.35 and 88.99% for the three grasses respectively. Similarly higher in vitro dry matter and organic digestibility coefficients were obtained from legume species than grasses. The study concluded that, the studied grasses were higher in fiber content and lower in their protein content but, legumes grasses (Vigna sunhum, Pennisetum Pedicelatum and Stylothanis flavicans) had high nutritive value and in vitro digestibility which may provide ruminants with the needed protein and energy.

scholarly journals Peningkatan Kualitas Jerami Padi MenggunakanTeknologi Amoniasi Fermentasi

2019 ◽  
Vol 2 (1) ◽  
pp. 96
Author(s):  
Muhamad Amin ◽  
Sofyan Damrah Hasan ◽  
Oscar Yanuarianto ◽  
Mohammad Iqbal ◽  
I Wayan Karda
Keyword(s):  
Organic Matter ◽  
Crude Protein ◽  
Dry Matter ◽  
Crude Fiber ◽  
In Vitro Digestibility ◽  
Randomized Design ◽  
Result Show ◽  
Completely Randomized Design ◽  
Range Test

An experiment with aim to study the effect of probiotics combination level and length of fermentation on qualityimprovement of rice straw had been conducted at the Laboratory of Nutrition and Feed of Animal HusbandryFaculty, Mataram University. The research was arranged in a Completely Randomized Design in factorialarrangement 3 x 3 with 3 replications. The first factor was the level of probiotics (0, 2 and 4%), the second factorwas the period of fermentation (2, 4 and 6 weeks).The variables measured were crude protein, crude fiber, NDF,ADF, cellulose, hemicellulose, lignin, and in vitro digestibility of dry matter and organic matter. Data wereanalyzed by analysis of variance and tested further by Duncan's Multiple Range Test. The result show thattreatments combination of the probiotics level and fermentation period did not affect (P>0.05) the chemicalcomposition and digestibility. The level of probiotics increased (P<0.05) the crude protein and in vitro dry matterdigestibility. Meanwhile, the lenght of fermentation increased the crude protein and in vitro digestibility of drymatter and organic matter, but decreased the crude fiber, NDF, ADF, cellulose, and hemicellulose of Amofer ricestraw.

EFFECT OF HARVEST DATES ON YIELD AND COMPOSITION OF FORAGE KALE

1989 ◽  
Vol 69 (1) ◽  
pp. 143-149 ◽  
Author(s):  
H. T. KUNELIUS ◽  
L. J. HALLIDAY ◽  
J. B. SANDERSON ◽  
U. C. GUPTA
Keyword(s):  
Brassica Oleracea ◽  
Crude Protein ◽  
Dry Matter ◽  
Lignin Content ◽  
In Vitro Digestibility ◽  
Cellulose Content ◽  
Cell Content ◽  
Harvest Dates ◽  
Brassica Oleracea L

Forage kale (Brassica oleracea L. ’Maris Kestrel’) was sown on 27 May and harvested at 18- to 22-d intervals between 16 Sept. and 6 Dec. in 1985 and 1986. Dry matter accumulated until early November and then declined at about 19 kg ha−1 d−1 until the final harvest. In vitro digestibility of dry matter (IVDDM) ranged from 916 to 952 g kg−1 among harvest dates. Crude protein was similar for most harvest dates. Hemi-cellulose content decreased in 1985 but not in 1986 while cellulose content decreased in 1986 but not in 1985. Lignin content increased from 25 to 43 g kg−1 between mid-September and early December. The cell content peaked at 814 g kg−1 in mid-November. There was an increase in S-methylcysteine sulphoxide (SMCO) concentration of leaves from 1.4 g kg−1 to 6.2 g kg−1 between the first and last harvest dates. Potassium concentrations increased in 1985 and decreased in 1986 from September to December while concentrations of P and S were not influenced by harvest dates. With later harvests, both Ca and Mg concentrations decreased while B decreased in 1985 but not in 1986. Zinc concentrations peaked in October in 1986 while in 1985 they were similar for the five harvest dates. Iron concentrations fluctuated in the two years while Cu and Mn concentrations were not influenced by harvest dates. Forage kale provided highly digestible dry matter in late season. The mineral composition of kale was adequate with the exception of Cu, Mn and Zn which would not satisfy the dietary requirements of ruminants. The SMCO concentrations in kale leaves increased steadily from mid-September to early December but were lower than those concentrations considered deleterious to animal health.Key words: Brassica oleracea L., crude protein, hemicellulose, cellulose, SMCO, macronutrients

Laboratory analyses of the nutritional value of western Queensland browse feeds

1979 ◽  
Vol 19 (98) ◽  
pp. 344 ◽  
Author(s):  
WJF McDonald ◽  
JH Ternouth
Keyword(s):  
Organic Matter ◽  
Crude Protein ◽  
Dry Matter ◽  
In Vitro Digestibility ◽  
Crude Fibre ◽  
Dry Matter Digestibility ◽  
Nitrogen Free Extract ◽  
Organic Matter Digestibility

Seventy samples of browse feeds, collected during late autumn and spring of 1971 from 42 western Queensland shrubs and trees, were subjected to laboratory analyses of the proximate fractions and in vitro digestibility. Crude protein, crude fibre and nitrogen-free-extract fractions ranged between 65 and 242, 55 and 353 and 425 and 71 1 g kg-1 dry matter, repectively. The in vitro dry matter digestibility coefficients ranged between 0.22 and 0.86. Comparisons of 15 browse feeds collected in April-May and October indicated a higher crude protein content (P < 0.05) and in vitro digestibility (P .= 0.01) and lower crude fibre (P < 0.01) and ash (P < 0.01 ) content in spring samples for most species. Nine browse feeds of known in vivo dry matter and organic matter digestibility were subjected to in vitro digestibility analysis. Whilst the in vitro and in vivo dry matter digestibilities were similar (0.43 and 0.45, respectively) the mean in vitro organic matter digestibility was substantially lower than the corresponding in vivo digestibility (0.40 and 0.46, respectively).

scholarly journals The Effect of Nutmeg Leaves Tannin (Myristica fragrans Houtt) as Protein Protecting Agents on In Vitro Nutrient Digestibility

2020 ◽  
Vol 44 (1) ◽  
Author(s):  
Monica Canadianti ◽  
Lies Mira Yusiati ◽  
Chusnul Hanim ◽  
Budi Prasetyo Widyobroto ◽  
Andriyani Astuti
Keyword(s):  
Organic Matter ◽  
Crude Protein ◽  
Dry Matter ◽  
Nutrient Digestibility ◽  
In Vitro Digestibility ◽  
Dry Matter Digestibility ◽  
In Vitro Technique ◽  
Significant Difference ◽  
Organic Matter Digestibility

This experiment was aimed to study the effect of nutmeg leaf tannin addition on in vitro nutrient digestibility. Treatments in this experiment consisted of: P0 (control without tannin), P1 (feed + 2% tannin) and P2 (feed + 4% tannin). Feed for fermentation substrate consisted of Pennisetum purpureum and soybean meal with ratio 60:40. Fermentation was carried out using Tilley and Terry two stages in vitro technique for 48 hours. Variables measured were the digestibility of dry matter, organic matter, and crude protein in the rumen as well as the total digestive tract digestibility based on in vitro technique. The data obtained were analyzed by One Way ANOVA, and followed by the Duncan Multiple Range Test (DMRT). The results showed that rumen dry matter digestibility was lower (P<0.05) in P1 and P2 (59.03±3.24 and 57.19±1.32) compared to P0 (70.77±1.05), but did not show a significant difference (P>0.05) in the total dry matter digestibility of P0, P1, and P2 (74.88±5.28, 67.70±3.21, and 64.83±4.96). Organic matter digestibility in the rumen was also lower (P<0.05) in P1 and P2 (55.55±6.29 and 55.76±6.88) compared to P0 (75.39±0.91), but did not show significant difference (P>0.05) in total organic matter digestibility from P0, P1, and P2 (64.69±6.44, 64.33±6.34, and 61.20±5.11). The digestibility of crude protein in the rumen at P1 and P2 (45.48±5.12 and 38.47±3.44) was also significantly lower (P<0.05) compared to P0 (60.93±9.72), whereas total digestibility did not show any significant difference (P>0.05). Addition of tannin leaf nutmeg 2% optimally reduced rumen dry matter and crude protein digestibility without causing excessive negative impact on results of in vitro digestibility, so it can be used as a protective agent protein feed.

scholarly journals 127 Porcine in vitro digestibility of whole stillage predigested with multi-enzyme during different time periods

2020 ◽  
Vol 98 (Supplement_3) ◽  
pp. 49-50
Author(s):  
Kevin S Jerez Bogota ◽  
Tofuko A Woyengo
Keyword(s):  
Dry Matter ◽  
In Vitro Digestion ◽  
Enzyme Treatment ◽  
Optimal Time ◽  
In Vitro Digestibility ◽  
Percentage Points ◽  
Whole Stillage ◽  
Freeze Dried ◽  
Different Sources

Abstract A study was conducted to determine the effects of the period of predigesting whole stillage (WS; slurry material that is dried into DDGS) with multi-enzyme and composition of the multi-enzyme on porcine in vitro digestibility of dry matter (IVDDM) of the WS. Four samples of whole stillage from 4 different sources were freeze-dried and divided into 13 subsamples to give 52 sub-samples. Thirteen treatments were applied to the 48 sub-samples within source. The treatments were undigested WS (control); or pre-digested with 1 of 3 multi-enzymes (MTE1, MTE2, and MTE3) at 55 °C for 6, 12, 18 or 24 h in 3 × 4 factorial arrangement. The MTE1 contained xylanase, β-glucanase, cellulase, mannanase, protease, and amylase; MTE2 contained xylanase, α-galactosidase, and cellulase; and MTE3 contained xylanase, cellulase, β-glucanase, and mannanase. The 52 subsamples were subjected to porcine in vitro digestion. The IVDDM of untreated WS was 73.3%. The IVDDM increased (P&lt; 0.05) with an increase in the predigestion period. However, a rise in the predigestion period from 0 to 12 h resulted in greater (P&lt; 0.05) response in mean IVDDM than an increment in the predigestion period from 12 to 24 h (11 vs. 0.83 percentage points). Predigestion period and multi-enzyme type interacted on IVDDM such that the improvement in IVDDM between 0 and 12 hours of predigestion differed (P&lt; 0.05) among the 3 multi-enzyme types (13.3, 11.1, and 8.5 percentage points for MTE3, MTE2, and MTE1, respectively). The LS means by multi-enzyme treatment were modeled and resulted in unparallel curves (P&lt; 0.05). The estimated maximum response of IVDDM for MTE1, MTE2 and MTE 3 were 82.4%, 84.7% and 87.1% at 15.8, 13 and 13.1 hours, respectively. In conclusion, the optimal time of predigestion of WS with multi-enzymes (with regard to improvement in its IVDDM) was approximately 14 h.

The assessment of herbage legume varieties. II. In vitro digestibility, water soluble carbohydrate, crude protein and mineral content of primary growth of clover and lucerne

1966 ◽  
Vol 66 (3) ◽  
pp. 351-357 ◽  
Author(s):  
W. Ellis Davies ◽  
G. ap Griffith ◽  
A. Ellington
Keyword(s):  
White Clover ◽  
Crude Protein ◽  
Dry Matter ◽  
Red Clover ◽  
Water Soluble ◽  
Soluble Carbohydrates ◽  
Soluble Carbohydrate ◽  
In Vitro Digestibility ◽  
Primary Growth

The primary growth of eight varieties of three species–white clover (3), red clover (4) and lucerne (1)–was sampled at fortnightly intervals and the percentage dry matter, in vitro digestibility, crude protein, water soluble carbohydrates, P, Ca, K, Na and Mg were determined.Differences between species were nearly always significant and the general order of merit was white clover, red clover and lucerne. The exceptions were for dry-matter percentage where this order was reversed, and red clover had the lowest Na and highest Mg content.

scholarly journals Effect of Phosphate Fertilizer and Arbuscular Mycorrhizal Fungi on The Nutrient, Phosphateuptake and in Vitro Digestibility of Alfalfa

2016 ◽  
Vol 40 (3) ◽  
pp. 203 ◽  
Author(s):  
Bambang Suwignyo ◽  
Bela Putra ◽  
Nafiatul Umami ◽  
Cahyo Wulandari ◽  
Ristianto Utomo
Keyword(s):  
Organic Matter ◽  
Mycorrhizal Fungi ◽  
Dry Matter ◽  
Arbuscular Mycorrhizal ◽  
Phosphate Fertilizer ◽  
P Uptake ◽  
In Vitro Digestibility ◽  
P Fertilizer ◽  
Total P

This study aimed to determine the effect of arbuscular mycorrhizal fungi (AMF) and phosphate (P) fertilizer on the nutrient content, phosphate uptake and in vitro digestibility of alfalfa (Medicago sativa L.).The research was conducted at green house of Forage and Pastures Science Laboratory, Faculty of Animal Science Universitas Gadjah Mada. The experiment was arranged in Completely Randomized Design using 3x4 factorial patterns with four replications. The first factor was dosage of phosphate fertilizer SP 36 (0, 60, and 120 kg/ha). Second factor was the dosage of AMF (0, 0.8, 1.6, and 2.4kg/ha). The variable measured was nutrient contents (crude protein, dry matter, and organic matter), total P uptake and dry matter and organic matter in vitro digestibility. The results showed that the interaction of AMF and P fertilizer had no significant effect on crude protein and total P uptake, but highly significant effect on the parameters of dry matter, organic matter and dry matter and organic matter in vitro digestibility. 

scholarly journals The influence of the method of preservation of forages on the digestion in dairy cows. 1. Composition of the forages and digestibility of dry matter, organic matter and nitrogen.

1975 ◽  
Vol 23 (1) ◽  
pp. 3-9
Author(s):  
S. Tamminga ◽  
C.J. van der Koelen
Keyword(s):  
Organic Matter ◽  
Formic Acid ◽  
Dairy Cows ◽  
Crude Protein ◽  
Dry Matter ◽  
Mixed Diet ◽  
Apparent Digestibility

1. Grass from the same sward was ensiled without additive, with 14.6 g formic acid/100 g crude protein or 10.8 g formic acid and 10.6 g formaldehyde/100 g crude protein. Similar grass was dried and pelleted. Drying or ensiling with the mixture reduced solubility of N in the preserved grass but formic acid increased it, and ensiling without additive increased it even more. Apparent digestibility of N in the rumen of cows tended to decrease with decrease in solubility. Digestibility in vitro of the mixed diet given to the cows, calculated from digestibility of the separate components, agreed well with the values in vivo for diets with silages, but was high for that with dried grass. (Abstract retrieved from CAB Abstracts by CABI’s permission)

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