REGRESSION ESTIMATES OF LYSINE AND OTHER AMINO ACIDS IN MANITOBA BARLEY

1990 ◽  
Vol 70 (1) ◽  
pp. 333-336 ◽  
Author(s):  
K. M. DUPCHAK ◽  
D. HICKLING
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Acid Content ◽  
Good Predictor ◽  
Crude Protein ◽  
Amino Acid Content ◽  
Prediction Equation ◽  
Regression Equations ◽  
Key Words Barley ◽  
Amino Acid Levels

Manitoba barley samples from the 1986 and 1987 crop years were analyzed for lysine and crude protein. The 1987 samples were also analyzed for other amino acids and for proximate components. Regression equations were developed to predict amino acid content. Crude protein was found to be a good predictor of amino acid levels. There was no effect of crop year on the prediction equation for lysine. Key words: Barley, lysine, prediction

Comparisons of amino acid and protein levels in oil-extracted seeds of Brassica and Sinapis species, with observations on environmental effects

2000 ◽  
Vol 80 (1) ◽  
pp. 169-174 ◽  
Author(s):  
J. M. Bell ◽  
G. Rakow ◽  
R. K. Downey
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Acid Content ◽  
Crude Protein ◽  
Amino Acid Content ◽  
Protein Levels ◽  
Protein Amino Acids ◽  
Amino Acid Levels ◽  
Lower Magnitude ◽  
Dispensable Amino Acids

Seeds from 21 selections of Brassica and Sinapis were analyzed for crude protein (CP; N × 6.25) and amino acid content following oil extraction and grinding. In exp. 1 three cultivars of B. napus, B. rapa and B. juncea were grown in four locations in 4 successive years, with duplicate samples taken from each plot. Brassica juncea contained more CP (P < 0.001) than B. napus or B. rapa (44.6 > 40.1 > 38.8%, respectively). Location affected CP level but not the ranking among cultivars. Year-to-year effects on CP levels (P < 0.001) were of lower magnitude than location effects. Treatments (cultivar, location, year) affected CP and amino acid levels differently. Amino acid levels expressed as percent of CP differed among many cultivars (P < 0.01). Threonine, valine, histidine, lysine and arginine were strongly influenced by location (P < 0.001), as were the dispensable amino acids serine, glutamine and tyrosine. In exp. 2, 10 cultivars and strains grown in one location and in three replicates resulted in greater CP levels in two B. juncea cultivars than in B. napus and B. rapa (42.9 and 43.2 vs. 39.7 and 38.8%), respectively, but lower levels of lysine and threonine in the CP (lysine: 5.4 vs. 6.3; threonine: 3.5 and 3.7 vs. 3.8 and 4.0%). Brassica napus and B. rapa ranked among the lowest in percent CP. In exp. 3, comparisons of eight samples from B. napus, B. rapa and Sinapis alba plots revealed a CP range of 38.1 to 45.0% but differences in amino acid content of the CP were relatively small. Key words: Brassica, Sinapis, protein, amino acids

scholarly journals Connection of protein and amino acid content of forage and medium quality winter wheat varieties

2009 ◽  
pp. 101-107
Author(s):  
Zoltán Mezei ◽  
Ágnes Pongrácznl Barancsi ◽  
Péter Sipos ◽  
Zoltán Győri ◽  
János Csapó
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Protein Content ◽  
Acid Content ◽  
Crude Protein ◽  
Amino Acid Content ◽  
Essential Amino Acids ◽  
Crude Protein Content ◽  
Wheat Varieties ◽  
Winter Wheat Varieties

We analysed the crude protein content, amino acid content, amino acid composition of four forage and milling III. quality winter wheat varieties (Magor, Hunor, Róna and Kondor) from their samples from five following years (2003, 2004, 2005, 2006, 2007). We found that quantity of essential and non-essential amino acids rose with increase in crude protein content. On examination of protein amino acid composition in relation to crude protein content we found that the crude protein content increased the quantities of the non-essential amino acids also rose, while those of the essential amino acids decreased as the lysine, the limiting amino acid of wheat. We also established that, as crude protein content increased, the biological value of the protein decreased.

scholarly journals Connection of crude protein content and amino acid content of forage and medium quality winter wheat varieties in the relation of quantity and quality

2008 ◽  
pp. 73-79
Author(s):  
Zoltán Mezei ◽  
Zoltán Győri ◽  
János Csapó
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Protein Content ◽  
Acid Content ◽  
Crude Protein ◽  
Amino Acid Content ◽  
Essential Amino Acids ◽  
Crude Protein Content ◽  
Wheat Varieties ◽  
Winter Wheat Varieties

We analysed the crude protein content, amino acid content, amino acid composition of four forage and milling III. quality winter wheat varieties (Magor, Hunor, Róna and Kondor) from their samples from five following years (2003, 2004, 2005, 2006, 2007). We found that quantity of essential and non-essential amino acids rose with increase in crude protein content. On examination of protein amino acid composition in relation to crude protein content we found that the crude protein content increased the quantities of the non-essential amino acids also rose, while those of the essential amino acids decreased as the lysine, the limiting amino acid of wheat. We also established that, as crude protein content increased, the biological value of the protein decreased.

scholarly journals The effect of selected cereals contained in feed ration on the amino acid composition of cows’ milk

2010 ◽  
Vol 58 (5) ◽  
pp. 369-376
Author(s):  
Markéta Šípalová ◽  
Oto Hanuš ◽  
František Buňka ◽  
Jan Pozdíšek ◽  
Vladimír Mrkvička ◽  
...  
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Dairy Cows ◽  
Nitrogen Content ◽  
Acid Content ◽  
Crude Protein ◽  
Amino Acid Content ◽  
Health Indicators ◽  
Control Group ◽  
Milk Samples

The aim of this study was to evaluate the possible effects of maize replacement in feeding rations on the amino acid content in cows´ milk. Cows were fed total mix ration based on the maize, clover silage and hay. There was a difference in the concentrate of the feeding ration. The first group (fed maize) was the control group, another two groups were experimental, one fed wheat and second fed triticale. During six weeks, totally 26 milk samples were taken from dairy cows of Czech Pied breed. Feed groups were preferably balanced in terms of milk yield, stage and number of lactations. The samples of feedstuffs as well as milk were modified for the analysis using acidic and oxidative hydrolyses. The analysis of amino acids content and composition of the sample hydrolysates was performed chromatographically by an AAA 400 analyzer, using Na-citrate buffers and ninhydrin detection. Total nitrogen content was determined according to Kjehldahl and the crude protein of the samples was determined by conversion from the nitrogen content multiplied by appropriate factor. The high content of crude protein in wheat did not influenced composition of milk from dairy cows fed this type of feedstuff. With respect to resulting amino acid content and composition of milk samples, none of the tested grains can be re­com­men­ded as a full-value maize replacement. Each feedstuff is an abundant source of several and ty­pi­cal amino acids in milk. However, triticale (cultivar Kitaro) seems to be acceptable replacement of maize owing to better crude protein efficiency, composition and health indicators of milk quality.

scholarly journals The Proximate Composition, Amino Acid Profile and Chemical Indices in Five Freshwater Fishes from Tiga Dam Reservoir, Nigeria

2019 ◽  
Vol 77 (2) ◽  
pp. 87-92
Author(s):  
Audu Michael Elaigwu
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Acid Content ◽  
Proximate Composition ◽  
Crude Protein ◽  
Amino Acid Content ◽  
Essential Amino Acids ◽  
Amino Acid Profile ◽  
High Energy ◽  
Dam Reservoir

Abstract The study assesses the proximate composition, amino acid profile and its content and chemical indices of the sun-dried Schilbe mystus, Bagrus bayad, Oreochromis niloticus, Clarias anguillaris and Petrocephalus bane bane from Tiga Dam Reservoir, Nigeria. The proximate composition varied significantly (p<0.05) in all five species of fish and these ranged as follows: Moisture (4.79 - 9.52 g/100 g), Crude Protein (42.20 - 57.71 g/100 g), Ash Content (0.90 - 12.51 g/100 g), Ether Extract (3.41 - 9.93 g/100 g), Crude Fibre (0.62 - 5.08 g/100 g), Nitrogen Free Extract (12.28 - 42.70 g/100 g) and Dry Matter (90.48 - 95.21 g/100 g). The amino acid also differed significantly (p<0.05). The nine essential amino acids found in the five species of fish were Lysine (4.21 - 6.34 g/100 g), Histidine (1.96 - 4.30 g/100 g), Arginine (5.80 - 8.21 g/100 g), Threonine (1.93 - 5.05 g/100 g), Valine (2.91 - 5.53 g/100 g), Methionine (1.74 - 3.80 g/100 g), Isoleucine (2.04 - 3.37 g/100 g), Leucine (3.64 - 7.18 g/100 g) and Phynylalanine (1.90 - 4.23 g/100 g). Whereas, the eight non-essential amino acids included: Serine (2.12 - 5.22 g/100 g), Glutamic acid (13.24 - 16.30 g/100 g), Proline (3.12 - 6.29 g/100 g), Glycine (4.20 - 9.08 g/100 g), Alanine (5.00 - 6.36 g/100 g), Cysteine (0.94 - 1.24 g/100 g), Tyrosine (2.33 - 3.33 g/100 g) and Aspartic acid (6.34 - 11.01 g/100 g). P. bane bane was first in terms of crude protein; S. mystus had the highest lipid and essential amino acid content. Also, C. anguillaris recorded the highest calorific value and best amino acid content. Thus, P. bane bane can serve as a source of animal protein to balance deficiencies in humans. Both S. mystus and C. anguillaris can be used as a nutrient base for high energy food and oil in the food industry.

scholarly journals Protein and Amino Acid Content in Four Brands of Commercial Table Eggs in Retail Markets in Relation to Human Requirements

Animals ◽  
2020 ◽  
Vol 10 (3) ◽  
pp. 406
Author(s):  
Youssef A. Attia ◽  
Mohammed A. Al-Harthi ◽  
Mohamed A. Korish ◽  
Mohamed H. Shiboob
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Acid Content ◽  
Crude Protein ◽  
Protein Quality ◽  
Amino Acid Content ◽  
Experimental Period ◽  
Essential Amino Acids ◽  
Retail Market ◽  
Edible Part

Considering the common believe that all eggs in the retail market are nutritionally similar, four different commercial sources of eggs (A, B, C, and D) available in a retail market were collected to investigate the crude protein and amino acid content, as well as the protein quality in the whole edible part of eggs (albumen + yolk), egg albumen, and egg yolk, separately. Five egg samples per source were collected four times during the experimental period, which resulted in a total number of 20 samples that were pooled to finally present five samples per source of eggs. The results show that crude protein in albumen was significantly higher in A and B than that of C and D, but the difference was found among edible parts of eggs such as yolk > whole edible part > albumen. Essential amino acids (arginine, histidine, isoleucine, lysine, methionine, methionine + cysteine, phenylalanine, phenylalanine + tyrosine, threonine, and valine) of eggs significantly differed according to the source of eggs, but eggs from different sources could provide from 17.4–26.7% of recommended daily allowance (RDA) of amino acids for adults. Essential amino acids (EAAs) were higher (p ≤ 0.05) in eggs from sources A and B than in source D, while source C exhibited intermediate values. Source B had greater (p ≤ 0.05) non-essential amino acids (NEAAs) than did sources C and D in whole edible egg, while source A displayed intermediate values. The phenylalanine + tyrosine, histidine, and lysine were the 1st, 2nd, and 3rd limiting amino acids in all sources of eggs. In conclusion, the investigated eggs showed different EAAs/NEAAs ratio and antioxidant amino acids, indicating a potential for enhancing nutritional values and extending the shelf life of eggs by different nutritional additions.

Evaluation of seed amino acid content and its correlation network analysis in wild soybean (Glycine soja) germplasm in Japan

2021 ◽  
Vol 19 (1) ◽  
pp. 35-43
Author(s):  
Awatsaya Chotekajorn ◽  
Takuyu Hashiguchi ◽  
Masatsugu Hashiguchi ◽  
Hidenori Tanaka ◽  
Ryo Akashi
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Free Amino Acid ◽  
Acid Content ◽  
Free Amino ◽  
Glycine Soja ◽  
Wild Soybean ◽  
Amino Acid Content ◽  
Soybean Seeds ◽  
Free Amino Acid Content

AbstractWild soybean (Glycine soja) is a valuable genetic resource for soybean improvement. Seed composition profiles provide beneficial information for the effective conservation and utilization of wild soybeans. Therefore, this study aimed to assess the variation in free amino acid abundance in the seeds of wild soybean germplasm collected in Japan. The free amino acid content in the seeds from 316 accessions of wild soybean ranged from 0.965 to 5.987 mg/g seed dry weight (DW), representing a 6.2-fold difference. Three amino acids had the highest coefficient of variation (CV): asparagine (1.15), histidine (0.95) and glutamine (0.94). Arginine (0.775 mg/g DW) was the predominant amino acid in wild soybean seeds, whereas the least abundant seed amino acid was glutamine (0.008 mg/g DW). A correlation network revealed significant positive relationships among most amino acids. Wild soybean seeds from different regions of origin had significantly different levels of several amino acids. In addition, a significant correlation between latitude and longitude of the collection sites and the total free amino acid content of seeds was observed. Our study reports diverse phenotypic data on the free amino acid content in seeds of wild soybean resources collected from throughout Japan. This information will be useful in conservation programmes for Japanese wild soybean and for the selection of accessions with favourable characteristics in future legume crop improvement efforts.

scholarly journals 24-Epibrassinolide Restores the Synthesis of Proteins and Amino Acids in Brassica juncea L. Leaves Under Imidacloprid Stress

2017 ◽  
Vol 25 (2) ◽  
pp. 85-90 ◽  
Author(s):  
Anket Sharma ◽  
Vinod Kumar ◽  
Ashwani Kumar Thukral ◽  
Renu Bhardwaj
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Brassica Juncea ◽  
Total Protein ◽  
Acid Content ◽  
Soil Amendment ◽  
Insect Pests ◽  
Amino Acid Content ◽  
Total Proteins ◽  
Amino Acid Contents

Abstract Pesticides are applied to protect crops from a variety of insect pests but their application cause toxicity to plants that results, among others, in reduction of protein as well as amino acid contents. The present study is aimed at observing the effect of seed pre-soaking with 24-epibrassinolide (EBL) on the protein and amino acid content in the leaves of Brassica juncea L. grown in soil that is amended with pesticide im-idacloprid (IMI). Soil amendment with IMI resulted in a decrease in the contents in leaves of total proteins and 21 amino acids studied. Seed soaking with 100 nM of EBL resulted in the recovery of total protein as well as amino acid contents in leaves, when compared with plants grown in only IMI amended soils.

The Oocytes of the Goby Pomatoschistus minutus. III. Determination of Amino Acid Component

1980 ◽  
Vol 35 (11-12) ◽  
pp. 1094-1095
Author(s):  
Rüdiger Riehl
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Glutamic Acid ◽  
Acid Content ◽  
Amino Acid Content ◽  
Acid Component ◽  
Pomatoschistus Minutus ◽  
Protein Amino Acids ◽  
Amino Acid Component

Abstract The oocytes of the marine goby Pomatoschistus minutus were analyzed for their amino acid content. Most of the amino acids exist as protein, only a little part is free or peptide-bound. Among the protein-bound amino acids, high levels of glutamic acid, proline, alanine, aspartic acid, valine and leucine were detected. These represent more than 60% of the protein amino acids. Among the free acids, glutamic acid, serine and alanine, are dominant. There are no certain proofs of the occurrence of peptide pools in the oocytes of Pomatoschistus minutus.

scholarly journals THE AMINO ACID COMPOSITION OF HYPERTENSIN II AND ITS BIOCHEMICAL RELATIONSHIP TO HYPERTENSIN I

1956 ◽  
Vol 104 (2) ◽  
pp. 183-191 ◽  
Author(s):  
Kenneth E. Lentz ◽  
Leonard T. Skeggs ◽  
Kenneth R. Woods ◽  
Joseph R. Kahn ◽  
Norman P. Shumway
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Ion Exchange ◽  
Amino Acid Composition ◽  
Acid Content ◽  
Amino Acid Content ◽  
Converting Enzyme ◽  
Terminal Sequence ◽  
Carboxyl Terminal ◽  
Hydrolysis Of

Preparations of hypertensin II, obtained from the treatment of hypertensin I by the action of the hypertensin converting enzyme of plasma and purified by countercurrent distribution, were quantitatively analyzed for their amino acid content. Chromatography on ion exchange columns showed the presence of equimolar amounts of aspartic acid, proline, valine, isoleucine, tyrosine, phenylalanine, histidine, and arginine. Hypertensin I was found to contain one mole of leucine and one mole of histidine in addition to the amino acids of hypertensin II. These two amino acids were isolated from the conversion products of hypertensin I and identified as the peptide histidylleucine. Carboxypeptidase digestion of hypertensin I showed the carboxyl terminal sequence of amino acids to be residue-phenylalanyl-histidylleucine. Similar studies of hypertensin II demonstrated residue-phenylalanine. It was concluded that the conversion of hypertensin I by the plasma hypertensin converting enzyme involved hydrolysis of the phenylalanyl-histidine bond to form hypertensin II and histidylleucine. The further removal by carboxypeptidase of phenylalanine from hypertensin II destroyed all of the vasoconstrictor activity.

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