A study of scab resistance in 16 apple progenies using parents with partial scab resistance

2009 ◽  
Vol 89 (4) ◽  
pp. 693-699 ◽  
Author(s):  
C R Hampson ◽  
H A Quamme ◽  
P L Sholberg
Keyword(s):  
Combining Ability ◽  
Partial Resistance ◽  
Venturia Inaequalis ◽  
Major Gene ◽  
Block Design ◽  
Apple Scab ◽  
Field Resistance ◽  
Specific Combining Ability ◽  
Scab Resistance ◽  
Breeding Value

A study was conducted to examine the inheritance of partial resistance to apple scab [Venturia inaequalis (Cke.) Wint.] using as parents a set of apple (Malus × domestica Borkh.) cultivars that show a range of field resistance to the disease, but are not known to carry any major-gene resistance. Akane, Alkmene, Antonovka, Carola, Chehalis, Goldgelbe, Katja and Summerred were used as pollen parents, and Spartan and an unnamed selection of Splendour × Gala parentage were used as seed parents in a factorial 2 × 8 mating design. One hundred seedlings of each family were planted in a randomized complete block design at a site with heavy annual natural scab infections. Each seedling was rated for infection severity for 3 consecutive years using a six-point categorical scale. A χ2 procedure was used to estimate the contribution of each parent to field resistance (analogous to general combining ability, GCA) and the contribution of the particular male-female combination (analogous to specific combining ability, SCA). Infection severity tended to increase over the 3 yr of the study. Antonovka and Chehalis had the highest analogous GCA for resistance (defined here as absence of sporulating lesions). Specific combining ability was present in 2 of 3 yr, but was small in magnitude relative to GCA. The field resistance of a given parent tended to be a poor predictor of its breeding value. However, 13 of the 16 families had at least 30% of seedlings with a potentially useful degree of partial resistance (low severity of infection). The results are discussed in the context of breeding apples with durable scab resistance.Key words: Fruit breeding, Malus, partial resistance, Venturia inaequalis

scholarly journals Inheritance studies of apple scab resistance and identification of Rvi14, a new major gene that acts together with other broad-spectrum QTL

Genome ◽  
2008 ◽  
Vol 51 (8) ◽  
pp. 657-667 ◽  
Author(s):  
V. Soufflet-Freslon ◽  
L. Gianfranceschi ◽  
A. Patocchi ◽  
C.-E. Durel
Keyword(s):  
Broad Spectrum ◽  
Quantitative Resistance ◽  
Venturia Inaequalis ◽  
Major Gene ◽  
Durable Resistance ◽  
Apple Scab ◽  
Genomic Region ◽  
Scab Resistance ◽  
Common Disease ◽  
Breeding Programmes

Scab, caused by the fungal pathogen Venturia inaequalis , is the most common disease of cultivated apple (Malus ×domestica). The fungal races 6 and 7 have now overcome the major resistance gene Vf, which is widely used in apple breeding programmes. New breeding strategies to achieve durable resistance are thus necessary. The aim of this study was to determine the genetic basis of quantitative resistance of the apple cultivar ‘Dülmener Rosenapfel’, known to be scab resistant under different environmental conditions. An F1 progeny derived from the cross between the susceptible cultivar ‘Gala’ and ‘Dülmener Rosenapfel’ was tested in a greenhouse with a multi-isolate inoculum of V. inaequalis. Rvi14, a new major gene that conditions a chlorotic-type reaction, was mapped on linkage group (LG) 6 in a genomic region not known to be involved in disease resistance. A further three quantitative trait loci (QTL) for resistance were identified. One co-localized with Rvi14 on LG6, whereas the remaining two were detected on LG11 and LG17, in genomic regions already reported to carry broad-spectrum QTL in other genetic backgrounds. Since a selective genotyping approach was used to detect QTL, an expectation-maximization (EM) computation was used to estimate the corrected QTL contributions to phenotypic variation and was validated by entire progeny genotyping.

scholarly journals Mapping Quantitative Field Resistance Against Apple Scab in a ‘Fiesta’ × ‘Discovery’ Progeny

2003 ◽  
Vol 93 (4) ◽  
pp. 493-501 ◽  
Author(s):  
R. Liebhard ◽  
B. Koller ◽  
A. Patocchi ◽  
M. Kellerhals ◽  
W. Pfammatter ◽  
...  
Keyword(s):  
Molecular Markers ◽  
Linkage Map ◽  
Genetic Linkage ◽  
Genetic Linkage Map ◽  
Venturia Inaequalis ◽  
Apple Scab ◽  
Interval Mapping ◽  
Mapping Method ◽  
Field Resistance ◽  
Scab Resistance

Breeding of resistant apple cultivars (Malus × domestica) as a disease management strategy relies on the knowledge and understanding of the underlying genetics. The availability of molecular markers and genetic linkage maps enables the detection and the analysis of major resistance genes as well as of quantitative trait loci (QTL) contributing to the resistance of a genotype. Such a genetic linkage map was constructed, based on a segregating population of the cross between apple cvs. Fiesta (syn. Red Pippin) and Discovery. The progeny was observed for 3 years at three different sites in Switzerland and field resistance against apple scab (Venturia inaequalis) was assessed. Only a weak correlation was detected between leaf scab and fruit scab. A QTL analysis was performed, based on the genetic linkage map consisting of 804 molecular markers and covering all 17 chromosomes of apple. With the maximum likelihood-based interval mapping method, eight genomic regions were identified, six conferring resistance against leaf scab and two conferring fruit scab resistance. Although cv. Discovery showed a much stronger resistance against scab in the field, most QTL identified were attributed to the more susceptible parent ‘Fiesta’. This indicated a high degree of homozygosity at the scab resistance loci in ‘Discovery’, preventing their detection in the progeny due to the lack of segregation.

scholarly journals Molecular Markers for the Scab Resistance (Vf) Region in Apple

1998 ◽  
Vol 123 (6) ◽  
pp. 992-996 ◽  
Author(s):  
Minou Hemmat ◽  
Norman F. Weeden ◽  
Herb S. Aldwinckle ◽  
Susan K. Brown
Keyword(s):  
Rapd Markers ◽  
Bulked Segregant Analysis ◽  
Venturia Inaequalis ◽  
Apple Scab ◽  
Scab Resistance ◽  
Resistant Plant ◽  
Tight Linkage ◽  
Test Population ◽  
Rate Selection ◽  
Selection Of

Bulked segregant analysis was used to identify RAPD markers that display tight linkage to the Vf gene in apple (Malus sp.) that confers resistance to five races of apple scab [Venturia inaequalis (Cke.) Wint.]. We identified several new RAPD markers linked to Vf. The most tightly linked marker in the test population, S52500, was cloned and sequenced. A linkage map of the Vf region was developed using these markers, RAPD markers previously described by other laboratories, and the isozyme locus Pgm-1. An assay was developed for Vf by multiplexing the two markers closely flanking the Vf locus. This assay has a theoretical `escape' value (discarding a resistant plant) of 3% and an error rate (selection of a susceptible plant) of 0.02%.

scholarly journals Using scab resistant donors in apple breeding

2017 ◽  
Vol 38 (SI 2 - 6th Conf EFPP 2002) ◽  
pp. 600-603
Author(s):  
B. Gelvonauskis ◽  
D. Gelvonauskienė
Keyword(s):  
Combining Ability ◽  
Apple Scab ◽  
General Combining Ability ◽  
Scab Resistance ◽  
Mean Square ◽  
Genetic Progress ◽  
Apple Cultivars ◽  
The Mean ◽  
Half Diallel ◽  
Selection Of

Apple cultivars were crossed according to the half-diallel and topcross mating design. Apple scab resistance of seedlings was estimated by using a 0–5 scale: 0 – no infection on leaves, 5 – more than 75% of leaf area infected. Percentage of scab resistant seedlings in crosses involving cultivars Prima varied from 35.8% to 67.6%, involving cultivars Florina and Arbat – 51.9–59.5% and 53.5–72.5%, respectively. Significant differences for resistance to scab were found among the crosses. Both general combining ability (GCA) and specific combining ability (SCA) were highly significant. The significance of GCA for scab resistance shows that genetic variability exists for this trait and selection should result in genetic progress, because the mean square for GCA was larger than that calculated for SCA. Therefore, selection of parents on its phenotype should be effective for development of resistant apple cultivars. The significant SCA estimates indicate that a large number of resistant seedlings from particular crosses should be selected.

scholarly journals The Rvi15 (Vr2) Apple Scab Resistance Locus Contains Three TIR-NBS-LRR Genes

2010 ◽  
Vol 23 (5) ◽  
pp. 608-617 ◽  
Author(s):  
Paolo Galli ◽  
Andrea Patocchi ◽  
Giovanni Antonio Lodovico Broggini ◽  
Cesare Gessler
Keyword(s):  
Venturia Inaequalis ◽  
Interleukin 1 ◽  
Apple Scab ◽  
Scab Resistance ◽  
Receptor Protein ◽  
Resistance Locus ◽  
Fresh Market ◽  
High Quality ◽  
Bac Clone ◽  
Bac Contig

Scab caused by the pathogen Venturia inaequalis is considered the most important fungal disease of cultivated apple (Malus × domestica Borkh.). In all, 16 monogenic resistances against scab have been found in different Malus spp. and some of them are currently used in apple breeding for scab-resistant cultivars. However, the self incompatibility and the long generation time of Malus spp. together with the high standards of fruit quality demanded from the fresh market render the breeding of high-quality cultivars in apple a long and expensive task. Therefore, the cloning of disease resistance genes and the use of the cloned genes for the transformation of high-quality apple cultivars could be an approach to solve these drawbacks. We report the construction of a bacterial artificial chromosome (BAC) contig spanning the Rvi15 (Vr2) apple scab resistance locus using two GMAL 2473 BAC libraries. A single BAC clone of the contig was sufficient to span the resistance locus. The BAC clone was completely sequenced, allowing identification of a sequence of 48.6 kb going from the two closest markers (ARGH17 and 77G20RP) bracketing Rvi15 (Vr2). Analysis of the 48.6-kb sequence revealed the presence of three putative genes characterized by a Toll and mammalian interleukin-1 receptor protein nucleotide-binding site leucine-rich repeat structure. All three genes were found to be transcribed.

scholarly journals Identification of Resistance to Multiple Fungicides in Field Populations of Venturia inaequalis

Plant Disease ◽  
2011 ◽  
Vol 95 (8) ◽  
pp. 921-926 ◽  
Author(s):  
Kimberly S. Chapman ◽  
George W. Sundin ◽  
Janna L. Beckerman
Keyword(s):  
Fungicide Resistance ◽  
Mycelial Growth ◽  
Venturia Inaequalis ◽  
Apple Scab ◽  
Field Resistance ◽  
Single Spore ◽  
Mycelium Growth ◽  
Thiophanate Methyl ◽  
Development Of Resistance

Venturia inaequalis, the causal agent of apple scab, is controlled primarily by fungicides. Long-term, extensive fungicide use has led to the development of resistance to multiple fungicides. To assess fungicide resistance, isolates of V. inaequalis were collected from Indiana and Michigan orchards. Single-spore derived isolates were evaluated by mycelium growth assays with previously determined discriminatory doses on media containing dodine, kresoxim-methyl, myclobutanil, or thiophanate-methyl. Of 195 isolates tested, 5.2, 0.7, 57.0, and 92.6% of isolates were found to be resistant to dodine, kresoxim-methyl, myclobutanil, and thiophanate-methyl, respectively. This is the first report of kresoxim-methyl field resistance in these states. Isolates resistant or shifted to a single fungicide were often found to have multiple fungicide resistance. Of all isolates tested, 38% were identified as resistant or shifted to two fungicides, and 12% were resistant or shifted to all four fungicides tested. No fitness penalty was found for isolates resistant to multiple fungicides based on a statistical analysis of mycelial growth and conidial production.

scholarly journals Molecular Markers for the Scab Resistance (Vf) Region in Apple

HortScience ◽  
1995 ◽  
Vol 30 (4) ◽  
pp. 855C-855 ◽  
Author(s):  
Minou Hemmat ◽  
Norman F. Weeden ◽  
Susan K. Brown
Keyword(s):  
Molecular Markers ◽  
Bulk Segregant Analysis ◽  
Rapd Markers ◽  
Venturia Inaequalis ◽  
Apple Scab ◽  
Genetic Resistance ◽  
Scab Resistance ◽  
Tight Linkage ◽  
Commercially Important ◽  
The Relationship

Apple scab, Venturia inaequalis (Cke.) Wint., is one of the most damaging diseases of apples. Although fungicide sprays have been used to control the disease, genetic resistance in existing commercially important varieties would be desirable. Identification of molecular marker(s) would be helpful in devising biotechnological approaches to control the disease. We used bulk segregant analysis to identify RAPD markers that cosegregate or display a tight linkage with Vf gene in Prima × Spartan cross. Using this approach, we are saturating the region around the scab resistance gene for the purpose of bracketing the locus. We have identified several markers associated with the Vf locus. The closest markers have been isolated and sequenced to be used as SCARs. The relationship and distances of the markers with the Vf locus and other previously reported markers will be discussed.

scholarly journals Combining ability studies for yield, associated traits and quality attributes in rice for South Gujarat (Oryza sativa L.)

2017 ◽  
Vol 9 (1) ◽  
pp. 60-67
Author(s):  
Navin Chander Gahtyari ◽  
P. I. Patel ◽  
Rakesh Choudhary ◽  
Sudhir Kumar ◽  
Naveen Kumar ◽  
...  
Keyword(s):  
Oryza Sativa ◽  
Grain Yield ◽  
Plant Height ◽  
Combining Ability ◽  
Amylose Content ◽  
Oryza Sativa L ◽  
Block Design ◽  
General Combining Ability ◽  
Specific Combining Ability ◽  
Kernel Length

Line x tester analysis using a set of four females, ten males and their forty hybrids was carried out to esti-mate the general combining ability of parents and specific combining ability of hybrids for yield and ten other associ-ated components in rice (Oryza sativa L.) in a Randomized Block Design during Kharif 2011. GCA variances for females (s2f) were significant at 0.1% level of significance for plant height (40.8), no. of grains per panicle (505.9), grain yield per plant (29.1), test weight(17.9), straw yield per plant (61.3) and kernel L/B ratio (0.2) whereas specific combining ability (SCA) variances for f x m interactions were highly significant for all the characters. Non-additive gene action was prevalent in all characters (Range: 0.03 in amylose content to 0.88 in kernel length breadth ratio) except plant height (1.33) as evident by low GCA to SCA ratio. None of the parents were good general combiner for all traits, however, female IR-28 and male AMT-119 and PNR-546 were good general combiners for a maximum number of traits i.e. five traits out of eleven.. The general combining ability for grain yield per plant for female parent Gurjari (6.19) and NVSR-178 (5.29); and male parents AMT 119 (2.73) and PNR 546 (2.44) makes them a good choice for improving yields in a hybridization programme as these female and male parents are also having signifi-cant GCA effects for maximum number of yield associated traits i.e. four and five traits out of eleven for female and male parents respectively. A vis-à-vis comparison of top three specific combining ability crosses with their mean performance showed correlation and these crosses were having at least one good general combining ability parent.

ASSESSMENT OF INITIAL MATERIAL OF AN APPLE TREE GENE POOL TO SCAB RESISTANCE

1970 ◽  
pp. 49-54
Author(s):  
N. G. Krasova ◽  
А. V. Pikunova ◽  
А. M. Galasheva
Keyword(s):  
Venturia Inaequalis ◽  
Field Resistance ◽  
Scab Resistance ◽  
Sources Of Resistance ◽  
Apple Varieties ◽  
Central Russia ◽  
Russian Research Institute ◽  
Monogenic Resistance ◽  
New Varieties

Apple (Malus. Mill.) germplasm has been studied for resistance to scab (Venturia inaequalis (Ske.) in the Central Russia at VNIISPK (Russian Research institute for Fruit Crop Breeding) for many years. Apple varieties with high field resistance to scab have been identified. Variety screening for DNA markers linked to genes of resistance made it possible to find among the new varieties of VNIISPK breeding digenic ones (Vf and Vm – Poezia, Va1 and Vf –Svezhest, Vm and Va1 – Zarianka, Patriot and Sokovinka) as well as sources of Vh2 (Reka) and Va1 (Antonovka Krasnobochka, Antonovka Obyknovennaya, Patriot, Orlik, Zarianka, Bessemianka Michurinskaya, Svezhest and Sokovinka). The genotyping confirmed the Vm presence in Zarianka, Orlovim, Patriot, Sokovinka and Chistotel and the Vf presence in Afrodita, Veniaminovskoye, Zhilinskoye, Zdorovie, Imrus, Rozhdestvenskoye, Svezhest, Solnyshko, Stroevskoye and Yubilar. Apple varieties that combine polygenic and monogenic resistance to scab – Imrus, Zdorovie and Svezhest are of great interest for practical use. The involvement of new sources of resistance will allow developing varieties with long-term resistance to scab.

scholarly journals Effects of Heat Stress on Growth, Physiology of Plants, Yield and Grain Quality of Different Spring Wheat (Triticum aestivum L.) Genotypes

2021 ◽  
Vol 13 (5) ◽  
pp. 2972
Author(s):  
Muhammad Waheed Riaz ◽  
Liu Yang ◽  
Muhammad Irfan Yousaf ◽  
Abdul Sami ◽  
Xu Dong Mei ◽  
...  
Keyword(s):  
Heat Stress ◽  
Grain Yield ◽  
Combining Ability ◽  
Grain Quality ◽  
Block Design ◽  
Stress Conditions ◽  
Specific Combining Ability ◽  
Growth Stages ◽  
Wheat Genotypes ◽  
Parental Lines

Heat stress is one of the major threats to wheat production in many wheat-growing areas of the world as it causes severe yield loss at the reproductive stage. In the current study, 28 crosses were developed using 11 parental lines, including 7 female lines and 4 male testers following line × tester matting design in 2018–2019. Twenty-eight crosses along with their 11 parental lines were sown in a randomized complete block design in triplicate under optimal and heat stress conditions. Fifteen different morpho-physiological and grain quality parameters were recorded at different growth stages. Analysis of variance illustrated the presence of highly significant differences among wheat genotypes for all traits under both optimal and heat stress conditions. The results of combining ability unveiled the predominant role of non-additive gene action in the inheritance of almost all the studied traits under both conditions. Among parents, 3 parental lines WL-27, WT-39, and WL-57 showed good combining ability under both normal and heat stress conditions. Among crosses, WL-8 × WT-17, WL-37 × WT-17, WL-7 × WT-39, and WL-37 × WT-39 portrayed the highest specific combining ability effects for grain yield and its related traits under optimal as well as heat stress conditions. Biplot and cluster analysis confirmed the results of general and specific combining ability by showing that these wheat crosses belonged to a highly productive and heat tolerant cluster. Correlation analysis revealed a significantly positive correlation of grain yield with net photosynthetic rate, thousand-grain rate, and the number of grains per spike. The designated parental lines and their crosses were selected for future breeding programs in the development of heat resilient, climate-smart wheat genotypes.

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