EFFECTS OF NITROGEN FERTILIZATION AND STALK POSITION ON CHLOROPHYLL, CAROTENOIDS, AND CERTAIN LIPIDS OF THREE TOBACCO GENOTYPES

1977 ◽  
Vol 57 (2) ◽  
pp. 525-535 ◽  
Author(s):  
C. GRUNWALD ◽  
J. L. SIMS ◽  
S. J. SHEEN
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Palmitic Acid ◽  
Free Fatty Acids ◽  
Fatty Acid Content ◽  
Total Sterol ◽  
N Fertilization ◽  
Major Fatty Acid ◽  
Fatty Acid Esters ◽  
Crude Lipid

Tobacco (Nicotiana tabacum L.) cv. Burley 21 and its breeding lines for low alkaloid, LA Burley 21, and low-polyphenol, LP Burley 21, were grown with rates of 112, 224, and 336 kg/ha of N fertilizer. Air-cured leaves from three stalk positions were analyzed for chlorophyll, carotenoid pigments, sterols, total and free fatty acids, and certain fatty acid esters. LA Burley 21 was highest in chlorophyll, carotenoids, and total sterols, while Burley 21 was lowest in chlorophyll and carotenoids and LP Burley 21 was lowest in sterols. The total fatty acid content was highest in LA Burley 21 and lowest in Burley 21. Palmitic acid was the major fatty acid of the free fatty acids and fatty acid ester groups. In general, N fertilization had only minor influences on the quantitative and qualitative composition of the lipid components. Stalk position, however, showed major effects. Crude lipid, chlorophyll, and carotenoids were higher in the top leaves than in bottom leaves. Total sterol content, however, was highest in bottom leaves, and free stigmasterol was the major component that accounted for this increase. The fatty acids in leaves from all stalk positions were similar from a quantitative and qualitative standpoint. A N fertilization × stalk position interaction was observed for total sterol and palmitic acid. Also, a genotype × stalk position interaction was found for contents of sterols, carotenoids, and chlorophyll.

scholarly journals Proximate composition and fatty acid content of the mangrove oyster Crassostrea rhizophorae along the year seasons

2004 ◽  
Vol 47 (6) ◽  
pp. 955-960 ◽  
Author(s):  
Ricardo Cavalcanti Martino ◽  
Gracinda Maria da Cruz
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Acid Content ◽  
Proximate Composition ◽  
Saturated Fatty Acids ◽  
Fatty Acid Content ◽  
Major Fatty Acid ◽  
Human Consumption ◽  
Crude Lipid ◽  
Crassostrea Rhizophorae

Mangrove oysters, Crassostrea rhizophorae were collected at the mangrove of "Barra de Guaratiba" district, Rio de Janeiro, Brazil, with the aim to determine the proximate composition and fatty acid content. Along the year seasons no statistical (P>0.05) difference was observed in the values of moisture, crude protein, crude lipid and ash. They were 82.0%; 9.7%; 1.7%; 3.2%, in average, respectively. However, glycogen was significantly (P<0.05) higher in spring (4.4%) and winter (4.2%) samples, than in summer (2.7%) and autumn (2.9%), samples. Saturated fatty acids and polyunsaturated fatty acids were respectively, the most important fatty acids in oysters, with the palmitic acid (16:0), being the major fatty acid. This study, demonstrated that this species was characterized by low fat content (< 2.0%) and also being a good source of eicosapentaenoic (20:5n-3, EPA) and docosahexaenoic (22:6n-3, DHA) fatty acids. Therefore, C. rhizophorae, in terms of lipid and fatty acids, could be recommended for human consumption.

scholarly journals Long-chain fatty acids of Sporothrix (Sporotrichum) schenckii

1976 ◽  
Vol 3 (6) ◽  
pp. 635-636
Author(s):  
R J Stretton ◽  
R K Dart
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Palmitic Acid ◽  
Acid Content ◽  
Fatty Acid Content ◽  
Sporothrix Schenckii ◽  
Major Fatty Acid ◽  
Long Chain Fatty Acids ◽  
Long Chain ◽  
Chain Fatty Acids

A number of strains purporting to belong to the species Sporothrix schenckii were examined for their fatty acid content. The majority of the strains were isolated from cases of sporotrichosis. Two strains were reputedly saprophytic. In all cases except the two saprophytic ones the major fatty acid was a C18 diene. Considerable amounts of palmitic acid and C18 monoene were found in all strains.

scholarly journals Fatty Acid Esters of Hydroxy Fatty Acids (FAHFAs) Are Associated With Diet, BMI, and Age

2021 ◽  
Vol 8 ◽  
Author(s):  
Teresa Kellerer ◽  
Karin Kleigrewe ◽  
Beate Brandl ◽  
Thomas Hofmann ◽  
Hans Hauner ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Weight Loss ◽  
Fatty Acid ◽  
Stearic Acid ◽  
Palmitic Acid ◽  
Fatty Acid Esters ◽  
Obese Patients ◽  
Hydroxy Stearic Acid ◽  
Diabetic Status ◽  
Acid Esters

Background: Fatty acid esters of hydroxy fatty acids (FAHFAs) are a group of fatty acids with potential anti-inflammatory and anti-diabetic effects. The blood levels of FAHFAs and their regulation in humans have hardly been studied.Objective: We aimed to investigate serum FAHFA levels in well-characterized human cohorts, to evaluate associations with age, sex, BMI, weight loss, diabetic status, and diet.Methods: We analyzed levels of stearic-acid-9-hydroxy-stearic-acid (9-SAHSA), oleic-acid-9-hydroxy-stearic-acid (9-OAHSA) and palmitic-acid-9-hydroxy-palmitic-acid (9-PAHPA) as well as different palmitic acid-hydroxy-stearic-acids (PAHSAs) by HPLC-MS/MS with the use of an internal standard in various cohorts: A cohort of different age groups (18–25y; 40–65y; 75–85y; Σn = 60); severely obese patients undergoing bariatric surgery and non-obese controls (Σn = 36); obese patients with and without diabetes (Σn = 20); vegetarians/vegans (n = 10) and omnivores (n = 9); and young men before and after acute overfeeding with saturated fatty acids (SFA) (n = 15).Results: Omnivores had substantially higher FAHFA levels than vegetarians/vegans [median (25th percentile; 75th percentile) tFAHFAs = 12.82 (7.57; 14.86) vs. 5.86 (5.10; 6.71) nmol/L; P &lt; 0.05]. Dietary overfeeding by supplementation of SFAs caused a significant increase within 1 week [median tFAHFAs = 4.31 (3.31; 5.27) vs. 6.96 (6.50; 7.76) nmol/L; P &lt; 0.001]. Moreover, obese patients had lower FAHFA levels than non-obese controls [median tFAHFAs = 3.24 (2.80; 4.30) vs. 5.22 (4.18; 7.46) nmol/L; P &lt; 0.01] and surgery-induced weight loss increased 9-OAHSA level while other FAHFAs were not affected. Furthermore, significant differences in some FAHFA levels were found between adolescents and adults or elderly, while no differences between sexes and between diabetic and non-diabetic individuals were detected.Conclusions: FAHFA serum levels are strongly affected by high SFA intake and reduced in severe obesity. Age also may influence FAHFA levels, whereas there was no detectable relation with sex and diabetic status. The physiological role of FAHFAs in humans remains to be better elucidated.Trial Registration: All studies referring to these analyses were registered in the German Clinical Trial Register (https://www.drks.de/drks_web/) with the numbers DRKS00009008, DRKS00010133, DRKS00006211, and DRKS00009797.

scholarly journals High rates of [1-14C]acetate incorporation into the lipid of isolated spinach chloroplasts

1976 ◽  
Vol 158 (3) ◽  
pp. 593-601 ◽  
Author(s):  
P G Roughan ◽  
C R Slack ◽  
R Holland
Keyword(s):  
Fatty Acids ◽  
Oleic Acid ◽  
Fatty Acid ◽  
Palmitic Acid ◽  
Free Fatty Acids ◽  
Acid Synthesis ◽  
Polar Lipids ◽  
Acetate Incorporation ◽  
Spinach Chloroplasts ◽  
Triton X

Spinach chloroplasts, isolated by techniques yielding preparations with high O2- evolving activity, showed rates of light-dependent acetate incorporation into lipids 3-4 fold higher than any previously reported. Incorporation rates as high as 500 nmol of acetate/h per mg of chlorophyll were measured in buffered sorbitol solutions containing only NaHCO3 and [1-14C]acetate, and as high as 800 nmol/h per mg of chlorophyll when 0.13 mM-Triton X-100 was also included in the reaction media. The fatty acids synthesized were predominantly oleic (70-80% of the total fatty acid radioactivity) and palmitic (20-25%) with only minor amounts (1-5%) of linoleic acid. Linolenic acid synthesis was not detected in the system in vitro. Free fatty acids accounted for 70-90% of the radioactivity incorporated and the remainder was shared fairly evenly between 1,2-diacylglycerols and polar lipids. Oleic acid constituted 80-90% of the free fatty acids synthesized, but the diacylglycerols and polar lipids contained slightly more palmitic acid than oleic acid. Triton X-100 stimulated the synthesis of diacylglycerols 3-6 fold, but stimulated free fatty acid synthesis only 1-1.5-fold. Added glycerol 1-phosphate stimulated both the synthesis of diacylglycerols and palmitic acid relative to oleic acid, but did not increase acetate incorporation into total chloroplast lipids. CoA and ATP, when added separately, stimulated acetate incorporation into chloroplast lipids to variable extents and had no effect on the types of lipid synthesized, but when added together resulted in 34% of the incorporated acetate appearing in long-chain acyl-CoA. Pyruvate was a much less effective precursor of chloroplast fatty acids than was acetate.

Reduction of Free Fatty Acid Content of Olive-Pomace Oil by Enzymatic Glycerolysis

2003 ◽  
Vol 9 (1) ◽  
pp. 11-15 ◽  
Author(s):  
S. Fadiloğlu ◽  
O. N. Çiftçi ◽  
F. Göğüş
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Free Fatty Acids ◽  
Acid Content ◽  
Molecular Sieve ◽  
Fatty Acid Content ◽  
Enzyme Concentration ◽  
Olive Pomace ◽  
Olive Pomace Oil ◽  
Acid Reduction

The enzymatic glycerolysis of free fatty acids in olive-pomace oil was carried out by immobilised Candida antarctica lipase. The effects of time, molecular sieve, enzyme concentration and reaction temperature on free fatty acids content were investigated. The initial acidity of the olive-pomace oil (32%) was reduced to 2.36% in the presence of 750 mg of molecular sieve in the reaction mixture. The effectiveness of glycerolysis was directly related to the amount of molecular sieve present. As the amount of molecular sieve increased, the conversion of free fatty acids also increased at a defined time. In the absence of molecular sieve, the esterification reaction forced to reverse reaction that is the hydrolysis. The greater conversion of free fatty acids into glycerides was observed at an enzyme concentration of 27.2 mg/mL within 60 min. ANOVA showed that the effects of temperature on fatty acid content was significant ( p < 0.05). Results obtained from non-linear regression analysis indicated that reaction order was 1.3 for fatty acid reduction in the olive-pomace oil. Calculated activation energy for fatty acid reduction was 32.89 kJ/mol.

Changes in free fatty acids during the ripening of Idiazabal cheese: influence of brining time and smoking

1994 ◽  
Vol 61 (2) ◽  
pp. 281-288 ◽  
Author(s):  
Ana I. Nájera ◽  
Luis J. R. Barron ◽  
Yolanda Barcina
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Free Fatty Acid ◽  
Free Fatty Acids ◽  
Free Fatty Acid Content ◽  
Acid Content ◽  
Lipolytic Activity ◽  
Fatty Acid Content ◽  
Ripening Period ◽  
Cheese Ripening

SummaryThe effect of brining time and smoking on the free fatty acid content of Idiazabal cheese during ripening was examined. The main free fatty acids considered underwent at least some increase during the first stage of ripening before day 90 and tended to level off around a constant value towards the end of the ripening period. There were significant differences in free fatty acid levels during ripening among cheeses with different brining times and between smoked and unsmoked cheeses. Brining time and smoking exerted marked effects on lipolytic activity during cheese ripening, depending upon the free fatty acid involved and ripening time. In general, brining and smoking led to increases in free fatty acid levels at the end of the ripening period; the different behaviour of butyric acid may be due to a specific lipolytic activity.

scholarly journals Oil yield and fatty acid profile of seeds of three Salvia species. A comparative study

2015 ◽  
Vol 61 (2) ◽  
pp. 14-29 ◽  
Author(s):  
Mouna Ben Farhat ◽  
Rym Chaouch -Hamada ◽  
Ahmed Landoulsi
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Polyunsaturated Fatty Acids ◽  
Comparative Study ◽  
Seed Oil ◽  
Unsaturated Fatty Acids ◽  
Fatty Acid Content ◽  
Fatty Acid Analysis ◽  
Major Fatty Acid ◽  
Oil Yield

Summary A comparative study of the oil yield and fatty acid composition of three Salvia species seeds collected in different locations has been conducted. Seed oil extraction was made using a Soxhlet-extractor and fatty acid analysis was undertaken using a GC-FID. The effect of the collecting site on oil yield, as well as the content of individual fatty acid and total fatty acid and fatty acid content was significant. Seed oil yield varied from 14.94 to 22.83% and the total fatty acids ranged from 67.36 to 82.49 mg/g DW. α-Linolenic (24.02-49.19%), linoleic (20.13-42.88%), oleic (12.97-17.81%) and palmitic (8.37-16.63%) acids were the most abundant fatty acids in all analyzed samples. α-Linolenic acid was found to be the major fatty acid in S. verbenaca and S. officinalis species, however, S. aegyptiaca was characterized by the prevalence of linoleic acid. Among the unsaturated fatty acids, which were represented in all samples in high amounts (78.16-89.34%), the polyunsaturated fatty acids (α-linolenic and linoleic acids) showed important levels ranging from 63.09 to 74.71%. Seeds of S. verbenaca were the richest in polyunsaturated fatty acids.

scholarly journals DECREASE IN FREE FATTY ACID CONTENT AND COLOR AT USED COOKING OIL WITH ACTIVATED CARBON OF REEDS (Imperata cylindrica L. Raeusch)

1970 ◽  
pp. 87-94
Author(s):  
Samsuar Samsuar
Keyword(s):  
Fatty Acids ◽  
Activated Carbon ◽  
Fatty Acid ◽  
Free Fatty Acid ◽  
Free Fatty Acids ◽  
Fatty Acid Content ◽  
Optimum Concentration ◽  
Imperata Cylindrica ◽  
Cooking Oil ◽  
Used Cooking Oil

Cooking oil is one of the foods that are needed by the community in daily life. The use of cooking oil continuously at high temperatures, produces cooking oil that is no longer feasible to use. Therefore, it’s necessary to purify used cooking oil so that it can be reused for non-foood purposes such as making soap or biodiesel fuel. This purification process is utilie the activated carbon of reeds (Imperata cylindrica L. Raeusch) as an adsorbent to reduce the levels of free fatty acids and colors in used cooking oil. Free fatty acid content test using acid base titration method and color change using Uv-Vis spectrofotometry method. This study aims to determine the optimum concentration of reeds activated carbon as an adsorbent in reducing the levels of free fatty acids and colors in used cooking oil, which consists of the concentration of reeds activated carbon which is a consentration of 2,5; 5; 7,5; 10; dan 12,5%. The results of variance analysis showed the optimum concentration of reeds activated carbon to reduce the levels of free fatty acids and colors absorbance in used cooking oil at a concentration of 7,5%. The percentage of decreasing levels of free fatty acids gorengan and pecel lele are 78.57% and 78.85%. Decrease in absorbance of gorengan color from 1,792% to 0,384% and the pecel lele color absorbance from 2,521 to 0,515. Keywords : Activated Carbon,Color, Free Fatty Acid, Reeds, and Used Cooking Oil.

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