Utilization of doubled haploid technique in Brassica rapa population improvement

2000 ◽  
Vol 80 (1) ◽  
pp. 75-82 ◽  
Author(s):  
H. Friesen ◽  
R. Scarth
Keyword(s):  
Brassica Rapa ◽  
Doubled Haploid ◽  
Rapd Analysis ◽  
Doubled Haploids ◽  
Agronomic Performance ◽  
Breeding Line ◽  
Synthetic Populations ◽  
Synthetic Population ◽  
Population Improvement ◽  
Dh Lines

The utilization of doubled haploid (DH) plants in population improvement of Brassica rapa was studied by randomly intercrossing 4, 8, 12 and 22 DH lines developed from the B. rapa cultivar Reward and the B. rapa breeding line DSC3 for two generations to constitute synthetic populations. The synthetic populations and the DH plants used in their formation were evaluated for agronomic performance at two locations in the field in 1996 and for genetic variation using random amplified polymorphic DNA (RAPD) analysis. Intercrossing as few as four DH lines from the breeding line DSC-3 produced a synthetic population with improved performance over that of the contributing DH lines. The synthetic population produced from the interpollination of eight DH lines showed an agronomic performance over that of the contributing DH lines to a level similar to the Reward donor population. RAPD analysis efficiently characterized the genotypic variation present in DH lines and synthetic populations, detecting 22–72% polymorphism between DH lines, 17–53% and 27–47% polymorphism in the first and second synthetic populations, respectively. This characterization may be useful as a tool in the reestablishment of heterogeneity and recovery of agronomic performance in B. rapa synthetic populations derived from DH lines by determining the level of genetic variability among DH lines and therefore optimal population size. Key words: Brassica rapa, synthetic population, doubled haploids, RAPD, agronomic performance

scholarly journals Doubled haploids of interspecific hybrids between Brassica napus and Brassica rapa for canola production with valuable breeding traits

OCL ◽  
2020 ◽  
Vol 27 ◽  
pp. 45
Author(s):  
Ainash Daurova ◽  
Dias Daurov ◽  
Dmitriy Volkov ◽  
Kuanysh Zhapar ◽  
Daniyar Raimbek ◽  
...  
Keyword(s):  
Brassica Napus ◽  
Seed Germination ◽  
Brassica Rapa ◽  
Doubled Haploid ◽  
Interspecific Hybrids ◽  
Nutritional Value ◽  
Doubled Haploids ◽  
Doubled Haploid Plants ◽  
Haploid Plants

Doubled haploids (DH) were obtained from two interspecific hybrids between Brassica napus and Brassica rapa. Seeds of doubled haploid plants differed in colour and size. The hybridity of the obtained doubled haploid is shown using genomic in situ hybridization (GISH) analysis. Evaluation of drought tolerance during seed germination on PEG-6000 showed the advantage of doubled haploid plants of interspecific hybrids over the parent cultivars. The oil from seeds of doubled haploid plants showed good nutritional value.

Growth and yield of doubled haploid lines of oilseed Brassica rapa

1998 ◽  
Vol 78 (4) ◽  
pp. 537-544 ◽  
Author(s):  
D. B. Dewan ◽  
G. Rakow ◽  
R. K. Downey
Keyword(s):  
Brassica Rapa ◽  
Seed Yield ◽  
Doubled Haploid ◽  
Field Tests ◽  
Field Evaluation ◽  
Growth And Yield ◽  
Consistent Performance ◽  
Delayed Flowering ◽  
Dh Lines ◽  
Number Of Seeds

The production of doubled haploid (DH) lines of Brassica rapa could be an efficient procedure for the development of inbred parents for hybrid production. A total of 162 B. rapa DH lines were evaluated in field tests at Saskatoon, Canada, in single row, replicated tests and 10 DH lines were tested in four-row plot, multilocation, replicated tests. Seed of DH lines was produced by bud selfing in the greenhouse. Approximately one-fifth of all DH lines tested were chlorophyll deficient, presumably due to the expression of recessive alleles. Inbreeding depression was evident in low seed and biological yields, low number of seeds per pod and delayed flowering. Seed yield of DH lines was positively associated with the number of seeds per pod, early flowering and a long pod-filling period. One DH line was equal in yield to its donor population (DP), suggesting that dominance deviation was the genetic basis for high seed yield in this species. The consistent performance of DH lines over years and locations indicated that DH lines may be selected after 1 year of evaluation for combining ability testing. Higher yielding DH lines of B. rapa must be selected before they can be used as parents for hybrid development. Key words: Brassica rapa, doubled haploid, field evaluation

Recurrent selection in doubled-haploid populations of barley (Hordeurn vulgare L.)

1985 ◽  
Vol 27 (2) ◽  
pp. 172-177 ◽  
Author(s):  
J. D. Patel ◽  
E. Reinbergs ◽  
S. O. Fejer
Keyword(s):  
Doubled Haploid ◽  
Recurrent Selection ◽  
Doubled Haploids ◽  
Additive Genetic Variance ◽  
Response To Selection ◽  
Hordeum Vulgare L ◽  
Doubled Haploid Lines ◽  
Days To Heading ◽  
High Selection ◽  
Dh Lines

Cycle zero (C0) of recurrent selection in barley (Hordeum vulgare L.) was initiated by diallel mating of seven highly selected parents. A total of 398 doubled-haploid (C0DH) lines were derived from 21 crosses and were evaluated along with their parents in C0 experiment. Seven doubled-haploid lines (DH) were selected from the cycle zero (C0) experiment and intercrossed to form cycle 1 (C1). From the 21 crosses of the diallel, 260 doubled-haploid lines (C1DH) were derived and were evaluated along with the C0 and C1 parents. The frequency distribution of the standardized means of the DH lines from C0 and C1 indicated a slight response to selection for seed yield. Genetic analysis of the C1DH population showed high additive genetic variance for yield per hill, plant height, and yield per spike, and a high proportion of additive × additive epistasis for spikes per hill, days to heading, and 100-seed weight. Seven doubled-haploid lines were selected from different high-yielding crosses represented by C1DH lines. High selection pressure was applied for yield per hill, yield per spike, and spikes per hill. Further response to selection is expected in later cycles. The seven selected doubled-haploid lines will be used as the parents of the next recurrent selection cycle.Key words: recurrent selection, doubled haploids, additive, epistasis, heritability, Hordeum.

scholarly journals Influence of Genotype on High Glucosinolate Synthesis Lines of Brassica rapa

2021 ◽  
Vol 22 (14) ◽  
pp. 7301
Author(s):  
Prabhakaran Soundararajan ◽  
Sin-Gi Park ◽  
So Youn Won ◽  
Mi-Sun Moon ◽  
Hyun Woo Park ◽  
...  
Keyword(s):  
Single Nucleotide Polymorphisms ◽  
Brassica Rapa ◽  
Doubled Haploid ◽  
Industrial Production ◽  
Dry Weight ◽  
Nucleotide Polymorphisms ◽  
Phenethyl Isothiocyanate ◽  
Single Nucleotide ◽  
Pak Choi ◽  
Dh Lines

This study was conducted to investigate doubled haploid (DH) lines produced between high GSL (HGSL) Brassica rapa ssp. trilocularis (yellow sarson) and low GSL (LGSL) B. rapa ssp. chinensis (pak choi) parents. In total, 161 DH lines were generated. GSL content of HGSL DH lines ranged from 44.12 to 57.04 μmol·g−1·dry weight (dw), which is within the level of high GSL B. rapa ssp. trilocularis (47.46 to 59.56 μmol g−1 dw). We resequenced five of the HGSL DH lines and three of the LGSL DH lines. Recombination blocks were formed between the parental and DH lines with 108,328 single-nucleotide polymorphisms in all chromosomes. In the measured GSL, gluconapin occurred as the major substrate in HGSL DH lines. Among the HGSL DH lines, BrYSP_DH005 had glucoraphanin levels approximately 12-fold higher than those of the HGSL mother plant. The hydrolysis capacity of GSL was analyzed in HGSL DH lines with a Korean pak choi cultivar as a control. Bioactive compounds, such as 3-butenyl isothiocyanate, 4-pentenyl isothiocyanate, 2-phenethyl isothiocyanate, and sulforaphane, were present in the HGSL DH lines at 3-fold to 6.3-fold higher levels compared to the commercial cultivar. The selected HGSL DH lines, resequencing data, and SNP identification were utilized for genome-assisted selection to develop elite GSL-enriched cultivars and the industrial production of potential anti-cancerous metabolites such as gluconapin and glucoraphanin.

scholarly journals Agronomic Performance and Selection of Doubled-Haploid Rice Lines for Rainfed Lowland Paddy Field

2020 ◽  
Vol 7 (03) ◽  
pp. 148-161
Author(s):  
Rojino Da Cunha ◽  
Bambang Sapta Purwoko ◽  
Iswari Saraswati Dewi ◽  
Willy Bayuardi Suwarno
Keyword(s):  
Doubled Haploid ◽  
Paddy Field ◽  
Block Design ◽  
Agronomic Performance ◽  
Rice Varieties ◽  
Days To Heading ◽  
Rainfed Lowland ◽  
Early Maturing ◽  
Dh Lines ◽  
Selection Of

Rainfed lowland rice cultivation is an alternative to increase national rice production. Breeding of high yielding rice varieties suitable for rainfed lowland condition can be accelerated by using doubled-haploid (DH) as genetic materials. This study aimed at obtaining information on the agronomic performance including yields in several DH rice lines and selecting DH lines suitable for rainfed lowland paddy field. The experimental design used was a Randomized Complete Block Design with 3 replications. The treatment was thirty DH lines and 4 check varieties namely RJ31 Ciherang, RJ32 Inpari 18, RJ33 Inpari 40, and RJ43 Inpari 41. The results showed that there were variability in all agronomic performances, i.e., plant height, number of tillers, days to heading and to harvest, panicle length, number of filled and empty grains, 1000-grain weight and grain yield. The DH lines, namely RJ19 DR8-43-3-1 and RJ25 DR10-14-1-1, gave the same productivity as 4 check varieties. Index selection showed that twelve DH lines with medium number of productive tillers, early maturing, and productivity of more than 4.40 tons.ha-1 were selected for further evaluation.

scholarly journals Grain size and other agronomical traits variation in a winter wheat population of doubled haploid lines

2020 ◽  
Vol 48 (3) ◽  
pp. 1369-1386
Author(s):  
Sorin CIULCA ◽  
Aurel GIURA ◽  
Adriana CIULCA
Keyword(s):  
Doubled Haploid ◽  
Doubled Haploids ◽  
Production Capacity ◽  
Wheat Breeding ◽  
Kernel Weight ◽  
Climatic Conditions ◽  
Climate Conditions ◽  
Wheat Population ◽  
Dh Lines ◽  
Agronomical Traits

In wheat, the size of the grain, respectively its dimensions as well as degree of filled, are important characteristics on which depends both the weight of the grain and yield of flour, the quality of milling and baking as well as the production capacity of the respective genotype. This paper presents the results obtained by studying for three years, under field condition, 85 doubled haploid (DH) lines obtained from the F1’s of ‘G.603-86’ (large grains genotype) × ‘F.132’ (normal grains genotype) crosses using biotechnological Zea system. The environmental conditions of the three years had an important contribution on the genotype × year interaction, which showed also a higher influence on 1000 kernel weight (TKW). The variability of plant height and ear emergence data was also affected to a similar extent by this interaction. Based on the performed results and analyses, were highlighted lines which show high and stable values of TKW (54-64 g), associated with a plants height of approximately 85-100 cm and an ear emergence from May 11 to 17, under some climatic conditions similar to the period of study. These doubled haploids lines can be considered as promising genotypes for using in wheat breeding programs in order to improve yield performances under temperate continental climate conditions.

scholarly journals Comparison of methods for obtaining doubled haploids of carrot

2017 ◽  
Vol 86 (2) ◽  
Author(s):  
Waldemar Kiszczak ◽  
Urszula Kowalska ◽  
Agata Kapuścińska ◽  
Maria Burian ◽  
Krystyna Górecka
Keyword(s):  
Plant Regeneration ◽  
Doubled Haploid ◽  
Aspartate Aminotransferase ◽  
Doubled Haploids ◽  
The Other ◽  
Plant Production ◽  
Comparison Of Methods ◽  
Anther Cultures ◽  
Induction Stage ◽  
Main Factor

Doubled haploid lines of carrot can be obtained through androgenesis in anther cultures and in isolated microspore cultures. The two methods were compared using three carrot cultivars (‘Kazan F1’, ‘Feria F1’, and ‘Narbonne F1’) at the androgenesis induction stage, during plant regeneration from embryos, and during acclimatization of androgenetic plants as well as their characterization. It was found that cultivar was the main factor affecting the efficiency at each stage of plant production in both anther and isolated microspore cultures. The efficiency of androgenesis in anther cultures of ‘Feria F1’ was considerably higher in comparison with isolated microspore cultures, and more plants were obtained from the embryos of androgenesis-cultured plants. In ‘Kazan F1’ and ‘Narbonne F1’, more acclimatized androgenetic plants were produced from anther cultures. Ploidy assessment of acclimatized plants of ‘Narbonne F1’ showed that the majority of the plants in the population derived from anther cultures had a doubled chromosome (DH) set. On the other hand, the majority of plants obtained from isolated microspore cultures were haploids. When assessing homozygosity, it was found among plants obtained in anther cultures that the percentage of homozygotes for phosphoglucose isomerase (PGI) and aspartate aminotransferase (AAT) depended on the cultivar. In contrast, the majority of plants derived from isolated microspore cultures were homozygous regardless of cultivar.

COMPARAISON ENTRE DES LIGNEES PEDIGREES ET DES LIGNEES HAPLOIDES DOUBLEES CHEZ L’ORGE (HORDEUM VULGARE L.)

1980 ◽  
Vol 60 (1) ◽  
pp. 79-85 ◽  
Author(s):  
PIERRE TURCOTTE ◽  
C. A. ST-PIERRE ◽  
KEH MING HO
Keyword(s):  
Hordeum Vulgare ◽  
Doubled Haploid ◽  
Doubled Haploids ◽  
Block Design ◽  
Pure Line ◽  
Kernel Weight ◽  
Hordeum Vulgare L ◽  
Line Production ◽  
Doubled Haploid Lines ◽  
Time Required

Pedigree and doubled haploid lines from seven crosses of barley (Hordeum vulgare L.) were compared over 2 years. The lines were tested, in a randomized complete block design, in row plots at Ste-Foy in 1977 and in hill plots at Brawley, California in 1978. There are significant differences between the two methods of pure line production for grain yield, 1000-kernel weight, plant height, resistance to lodging and date of maturity. Furthermore, these significant differences between doubled haploid and pedigree lines seem to be tied to wide crosses. After showing that doubled haploids are superior for resistance to lodging, we conclude that the use of doubled haploid lines must be seriously considered in a barley breeding program on the basis of improved selection efficiency and the short time required to get homozygous lines.

Agronomic Performance of Wheat Doubled-Haploid Lines Derived from Cultivars by Anther Culture

1989 ◽  
Vol 103 (2) ◽  
pp. 101-109 ◽  
Author(s):  
P. S. Baenziger ◽  
D. M. Wesenberg ◽  
V. M. Smail ◽  
W. L. Alexander ◽  
G. W. Schaeffer
Keyword(s):  
Anther Culture ◽  
Doubled Haploid ◽  
Agronomic Performance ◽  
Doubled Haploid Lines

Haploidy and plant breeding

1981 ◽  
Vol 292 (1062) ◽  
pp. 499-507 ◽  
Keyword(s):  
Plant Breeding ◽  
Doubled Haploids ◽  
Chromosome Complement ◽  
Basic Research ◽  
Breeding Value ◽  
Additive Variance ◽  
Ploidy Levels ◽  
Breeding Programmes ◽  
Dh Lines

A haploid is an organism that looks like a sporophyte, but has the chromosome complement of a reduced gamete. There are several ways in which haploids can occur or be induced in vivo : spontaneously, mostly associated with polyembryony, and through abnormal processes after crosses, like pseudogamy, semigamy, preferential elimination of the chromosomes of one parental species, and androgenesis. In the crops described, haploids are or are near to being used in basic research and plant breeding. The application of haploids in breeding self-pollinated crops is based on their potential for producing fully homozygous lines in one generation, which can be assessed directly in the field. Early generation testing of segregating populations is possible through haploids, because doubled haploids (DH) possess additive variance only. Haploids can also be applied in classical breeding programmes to make these more efficient through improved reliability of selection. The application of haploids in cross-pollinated crops is also based on a rapid production of DH-lines, which can be used as inbred lines for the production of hybrid varieties. By means of haploids all natural barriers to repeated selfing are bypassed. In autotetraploid crops there are two types of haploid. One cycle of haploidization leads to dihaploids; a second cycle produces monohaploids. The significance of dihaploids is in their greatly simplified genetics and breeding and in the possibility of estimation of the breeding value of tetraploid cultivars by assessing their dihaploids. The main drawback of dihaploids is their restriction to two alleles per locus. Also, after doubling, it is impossible to achieve tetra-allelism at many loci, the requirement for maximal performance of autotetraploid cultivars. Tetra-allelism can be obtained when improved dihaploids have a genetically controlled mechanism of forming highly heterozygous restitution gametes with the unreduced number of chromosomes. Monohaploids, after doubling or twice doubling, may lead to fully homozygous diploids and tetraploids. These are important for basic research, but not yet for practical application. Meiotic data of potato homozygotes at three ploidy levels are presented.

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